Experiment: Nitrate reduction, replicate A t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5726 and Pf1N1B4_5727 are separated by 15 nucleotides Pf1N1B4_5727 and Pf1N1B4_5728 are separated by 5 nucleotides Pf1N1B4_5728 and Pf1N1B4_5729 are separated by 32 nucleotides
Pf1N1B4_5726 - Ferredoxin, at 165,339 to 166,667
_5726
Pf1N1B4_5727 - Ribonucleotide reductase of class III (anaerobic), activating protein (EC 1.97.1.4), at 166,683 to 167,369
_5727
Pf1N1B4_5728 - hypothetical protein, at 167,375 to 167,542
_5728
Pf1N1B4_5729 - Ribonucleotide reductase of class III (anaerobic), large subunit (EC 1.17.4.2), at 167,575 to 169,590
_5729
Position (kb) 167
168 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 166.511 kb on - strand, within Pf1N1B4_5726 at 166.511 kb on - strand, within Pf1N1B4_5726 at 166.906 kb on + strand, within Pf1N1B4_5727 at 167.781 kb on - strand, within Pf1N1B4_5729 at 167.781 kb on - strand, within Pf1N1B4_5729 at 167.781 kb on - strand at 167.781 kb on - strand, within Pf1N1B4_5729 at 167.891 kb on + strand, within Pf1N1B4_5729 at 167.891 kb on + strand, within Pf1N1B4_5729 at 167.891 kb on + strand, within Pf1N1B4_5729 at 167.894 kb on + strand, within Pf1N1B4_5729 at 167.999 kb on + strand at 168.094 kb on - strand, within Pf1N1B4_5729 at 168.194 kb on + strand, within Pf1N1B4_5729 at 168.194 kb on + strand, within Pf1N1B4_5729 at 168.320 kb on + strand, within Pf1N1B4_5729 at 168.320 kb on + strand, within Pf1N1B4_5729
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1 remove 166,511 - Pf1N1B4_5726 0.88 +0.0 166,511 - Pf1N1B4_5726 0.88 -2.4 166,906 + Pf1N1B4_5727 0.32 +0.0 167,781 - Pf1N1B4_5729 0.10 +0.0 167,781 - Pf1N1B4_5729 0.10 +0.0 167,781 - +0.0 167,781 - Pf1N1B4_5729 0.10 -5.0 167,891 + Pf1N1B4_5729 0.16 +0.0 167,891 + Pf1N1B4_5729 0.16 +0.0 167,891 + Pf1N1B4_5729 0.16 -2.7 167,894 + Pf1N1B4_5729 0.16 +0.0 167,999 + +0.0 168,094 - Pf1N1B4_5729 0.26 +0.0 168,194 + Pf1N1B4_5729 0.31 -3.6 168,194 + Pf1N1B4_5729 0.31 -3.9 168,320 + Pf1N1B4_5729 0.37 +0.0 168,320 + Pf1N1B4_5729 0.37 -2.8
Or see this region's nucleotide sequence