Experiment: Nitrate reduction, replicate A t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5663 and Pf1N1B4_5664 are separated by 191 nucleotides Pf1N1B4_5664 and Pf1N1B4_5665 are separated by 291 nucleotides Pf1N1B4_5665 and Pf1N1B4_5666 overlap by 17 nucleotides Pf1N1B4_5666 and Pf1N1B4_5667 are separated by 44 nucleotides
Pf1N1B4_5663 - Periplasmic sugar-binding domain protein, at 97,139 to 98,149
_5663
Pf1N1B4_5664 - hypothetical protein, at 98,341 to 98,454
_5664
Pf1N1B4_5665 - Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2), at 98,746 to 100,404
_5665
Pf1N1B4_5666 - Oxidoreductase probably involved in sulfite reduction, at 100,388 to 100,882
_5666
Pf1N1B4_5667 - hypothetical protein, at 100,927 to 104,862
_5667
Position (kb) 98
99
100
101 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 97.789 kb on - strand, within Pf1N1B4_5663 at 97.789 kb on - strand, within Pf1N1B4_5663 at 97.789 kb on - strand, within Pf1N1B4_5663 at 97.837 kb on - strand, within Pf1N1B4_5663 at 97.950 kb on + strand, within Pf1N1B4_5663 at 97.950 kb on + strand, within Pf1N1B4_5663 at 98.103 kb on + strand at 98.192 kb on + strand at 98.208 kb on + strand at 98.209 kb on - strand at 98.209 kb on - strand at 98.260 kb on - strand at 98.260 kb on - strand at 98.342 kb on + strand at 98.343 kb on - strand at 98.609 kb on - strand at 98.622 kb on + strand at 98.623 kb on - strand at 98.623 kb on - strand at 98.709 kb on + strand at 98.710 kb on - strand at 98.710 kb on - strand at 98.743 kb on + strand at 98.743 kb on + strand at 98.743 kb on + strand at 98.743 kb on + strand at 98.743 kb on + strand at 98.743 kb on + strand at 98.743 kb on + strand at 98.743 kb on + strand at 98.743 kb on + strand at 98.744 kb on - strand at 98.744 kb on - strand at 98.744 kb on - strand at 98.744 kb on - strand at 98.744 kb on - strand at 98.744 kb on - strand at 98.744 kb on - strand at 98.744 kb on - strand at 98.744 kb on - strand at 98.745 kb on + strand at 98.745 kb on + strand at 98.745 kb on + strand at 98.745 kb on + strand at 98.745 kb on + strand at 98.745 kb on + strand at 98.745 kb on + strand at 98.745 kb on + strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.749 kb on + strand at 98.749 kb on + strand at 98.749 kb on + strand at 98.750 kb on - strand at 98.750 kb on - strand at 98.750 kb on - strand at 98.750 kb on - strand at 98.750 kb on - strand at 98.750 kb on - strand at 98.750 kb on - strand at 98.750 kb on - strand at 98.753 kb on + strand at 98.753 kb on + strand at 98.753 kb on + strand at 98.754 kb on - strand at 98.754 kb on - strand at 98.754 kb on - strand at 98.754 kb on - strand at 98.754 kb on - strand at 98.755 kb on + strand at 98.756 kb on - strand at 98.756 kb on - strand at 98.764 kb on + strand at 98.881 kb on + strand at 98.881 kb on + strand at 98.882 kb on - strand at 98.882 kb on - strand at 98.882 kb on - strand at 98.882 kb on - strand at 99.238 kb on - strand, within Pf1N1B4_5665 at 99.430 kb on + strand, within Pf1N1B4_5665 at 99.430 kb on + strand, within Pf1N1B4_5665 at 99.430 kb on + strand, within Pf1N1B4_5665 at 99.431 kb on - strand, within Pf1N1B4_5665 at 99.455 kb on - strand, within Pf1N1B4_5665 at 99.484 kb on + strand, within Pf1N1B4_5665 at 99.484 kb on + strand at 99.485 kb on - strand, within Pf1N1B4_5665 at 99.485 kb on - strand, within Pf1N1B4_5665 at 99.745 kb on + strand, within Pf1N1B4_5665 at 100.075 kb on + strand, within Pf1N1B4_5665 at 100.076 kb on - strand, within Pf1N1B4_5665 at 100.192 kb on + strand, within Pf1N1B4_5665 at 100.192 kb on + strand, within Pf1N1B4_5665 at 100.192 kb on + strand, within Pf1N1B4_5665 at 100.306 kb on + strand at 100.306 kb on + strand at 100.306 kb on + strand at 100.306 kb on + strand at 100.306 kb on + strand at 100.306 kb on + strand at 100.348 kb on + strand at 100.348 kb on + strand at 100.348 kb on + strand at 100.348 kb on + strand at 100.348 kb on + strand at 100.349 kb on - strand at 100.388 kb on - strand at 100.399 kb on - strand at 100.399 kb on - strand at 100.402 kb on + strand at 100.402 kb on + strand at 100.402 kb on + strand at 100.403 kb on - strand at 100.522 kb on + strand, within Pf1N1B4_5666 at 100.655 kb on + strand, within Pf1N1B4_5666 at 100.655 kb on + strand, within Pf1N1B4_5666 at 100.655 kb on + strand, within Pf1N1B4_5666 at 100.742 kb on + strand, within Pf1N1B4_5666 at 100.742 kb on + strand, within Pf1N1B4_5666 at 100.742 kb on + strand, within Pf1N1B4_5666 at 100.796 kb on + strand, within Pf1N1B4_5666 at 100.835 kb on + strand at 100.835 kb on + strand at 100.928 kb on + strand at 100.929 kb on - strand at 101.136 kb on + strand at 101.136 kb on + strand at 101.136 kb on + strand at 101.137 kb on - strand at 101.137 kb on - strand at 101.139 kb on - strand at 101.139 kb on - strand at 101.139 kb on - strand at 101.270 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1 remove 97,789 - Pf1N1B4_5663 0.64 +0.0 97,789 - Pf1N1B4_5663 0.64 +0.0 97,789 - Pf1N1B4_5663 0.64 +0.0 97,837 - Pf1N1B4_5663 0.69 +0.0 97,950 + Pf1N1B4_5663 0.80 +0.0 97,950 + Pf1N1B4_5663 0.80 +0.0 98,103 + +0.0 98,192 + -1.1 98,208 + +0.0 98,209 - +0.0 98,209 - +0.0 98,260 - -3.6 98,260 - -1.3 98,342 + +0.0 98,343 - -1.0 98,609 - -5.6 98,622 + -0.8 98,623 - +0.0 98,623 - -3.9 98,709 + +0.0 98,710 - +0.0 98,710 - -5.3 98,743 + -2.3 98,743 + +0.0 98,743 + +0.0 98,743 + +0.0 98,743 + +0.0 98,743 + -2.2 98,743 + +0.0 98,743 + -3.3 98,743 + -3.0 98,744 - -2.8 98,744 - -3.2 98,744 - +0.0 98,744 - +0.0 98,744 - +0.0 98,744 - -4.9 98,744 - -0.4 98,744 - -4.1 98,744 - +0.0 98,745 + +0.0 98,745 + -3.0 98,745 + +0.0 98,745 + +0.0 98,745 + -2.8 98,745 + +0.0 98,745 + +0.0 98,745 + -3.5 98,746 - +0.0 98,746 - +0.0 98,746 - +0.0 98,746 - +0.0 98,746 - -3.3 98,746 - +0.0 98,746 - -1.8 98,746 - -2.8 98,746 - +0.0 98,746 - -3.6 98,746 - +0.0 98,746 - -3.7 98,746 - +0.0 98,746 - -3.0 98,746 - -3.5 98,749 + -4.4 98,749 + -3.2 98,749 + -2.1 98,750 - -3.3 98,750 - -2.2 98,750 - +0.0 98,750 - -5.2 98,750 - -4.1 98,750 - -3.6 98,750 - -3.1 98,750 - +0.0 98,753 + -3.0 98,753 + -3.1 98,753 + +0.0 98,754 - +0.0 98,754 - -1.5 98,754 - +0.0 98,754 - +0.0 98,754 - +0.0 98,755 + -3.4 98,756 - +0.0 98,756 - +0.0 98,764 + -1.0 98,881 + -3.9 98,881 + +0.0 98,882 - +0.0 98,882 - +0.0 98,882 - +0.0 98,882 - -2.3 99,238 - Pf1N1B4_5665 0.30 +0.0 99,430 + Pf1N1B4_5665 0.41 -4.0 99,430 + Pf1N1B4_5665 0.41 -2.1 99,430 + Pf1N1B4_5665 0.41 +0.0 99,431 - Pf1N1B4_5665 0.41 +1.0 99,455 - Pf1N1B4_5665 0.43 -3.2 99,484 + Pf1N1B4_5665 0.44 -3.7 99,484 + +0.0 99,485 - Pf1N1B4_5665 0.45 +0.0 99,485 - Pf1N1B4_5665 0.45 +0.0 99,745 + Pf1N1B4_5665 0.60 -3.4 100,075 + Pf1N1B4_5665 0.80 +0.0 100,076 - Pf1N1B4_5665 0.80 -4.7 100,192 + Pf1N1B4_5665 0.87 -3.6 100,192 + Pf1N1B4_5665 0.87 -2.0 100,192 + Pf1N1B4_5665 0.87 +0.0 100,306 + +0.0 100,306 + -3.0 100,306 + +0.0 100,306 + -1.9 100,306 + -2.6 100,306 + -3.0 100,348 + -4.5 100,348 + +0.0 100,348 + +0.0 100,348 + -3.0 100,348 + -4.7 100,349 - +0.0 100,388 - -2.2 100,399 - -2.7 100,399 - +0.0 100,402 + -3.6 100,402 + +0.0 100,402 + +0.0 100,403 - +0.0 100,522 + Pf1N1B4_5666 0.27 +0.0 100,655 + Pf1N1B4_5666 0.54 +0.0 100,655 + Pf1N1B4_5666 0.54 +0.0 100,655 + Pf1N1B4_5666 0.54 -2.2 100,742 + Pf1N1B4_5666 0.72 -3.1 100,742 + Pf1N1B4_5666 0.72 +0.0 100,742 + Pf1N1B4_5666 0.72 +0.0 100,796 + Pf1N1B4_5666 0.82 -4.6 100,835 + -2.4 100,835 + +0.0 100,928 + +0.0 100,929 - +0.0 101,136 + -4.0 101,136 + +0.0 101,136 + -1.4 101,137 - +0.0 101,137 - +0.0 101,139 - -1.5 101,139 - +0.0 101,139 - -2.2 101,270 - -2.0
Or see this region's nucleotide sequence