Experiment: Nitrate reduction, replicate A t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5618 and Pf1N1B4_5619 are separated by 2 nucleotides Pf1N1B4_5619 and Pf1N1B4_5620 are separated by 184 nucleotides Pf1N1B4_5620 and Pf1N1B4_5621 are separated by 0 nucleotides
Pf1N1B4_5618 - Excinuclease ABC subunit C, at 44,031 to 45,854
_5618
Pf1N1B4_5619 - BarA-associated response regulator UvrY (= GacA = SirA), at 45,857 to 46,441
_5619
Pf1N1B4_5620 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 46,626 to 47,069
_5620
Pf1N1B4_5621 - Hydrolase, carbon-nitrogen family, at 47,070 to 47,828
_5621
Position (kb) 46
47
48 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 45.718 kb on + strand at 45.761 kb on - strand at 45.761 kb on - strand at 45.955 kb on + strand, within Pf1N1B4_5619 at 45.955 kb on + strand, within Pf1N1B4_5619 at 46.128 kb on + strand at 46.128 kb on + strand, within Pf1N1B4_5619 at 46.129 kb on - strand, within Pf1N1B4_5619 at 46.485 kb on - strand at 46.493 kb on - strand at 46.522 kb on + strand at 46.580 kb on + strand at 46.647 kb on - strand at 46.837 kb on - strand, within Pf1N1B4_5620 at 46.856 kb on - strand, within Pf1N1B4_5620 at 46.889 kb on - strand, within Pf1N1B4_5620 at 47.135 kb on + strand at 47.136 kb on - strand at 47.273 kb on - strand, within Pf1N1B4_5621 at 47.766 kb on - strand at 47.891 kb on + strand at 47.958 kb on + strand at 47.958 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1 remove 45,718 + +0.0 45,761 - -1.3 45,761 - -1.3 45,955 + Pf1N1B4_5619 0.17 +3.0 45,955 + Pf1N1B4_5619 0.17 +0.0 46,128 + +0.0 46,128 + Pf1N1B4_5619 0.46 +0.0 46,129 - Pf1N1B4_5619 0.46 +2.5 46,485 - +2.7 46,493 - +0.0 46,522 + +0.0 46,580 + +0.0 46,647 - -1.1 46,837 - Pf1N1B4_5620 0.48 +0.0 46,856 - Pf1N1B4_5620 0.52 -2.6 46,889 - Pf1N1B4_5620 0.59 -2.2 47,135 + +0.0 47,136 - +0.0 47,273 - Pf1N1B4_5621 0.27 +0.0 47,766 - -2.4 47,891 + -1.0 47,958 + -3.1 47,958 + +0.0
Or see this region's nucleotide sequence