Experiment: Nitrate reduction, replicate A t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5510 and Pf1N1B4_5511 are separated by 910 nucleotides Pf1N1B4_5511 and Pf1N1B4_5512 are separated by 342 nucleotides Pf1N1B4_5512 and Pf1N1B4_5513 are separated by 75 nucleotides Pf1N1B4_5513 and Pf1N1B4_5514 are separated by 69 nucleotides
Pf1N1B4_5510 - hypothetical protein, at 259,450 to 259,563
_5510
Pf1N1B4_5511 - hypothetical protein, at 260,474 to 260,755
_5511
Pf1N1B4_5512 - hypothetical protein, at 261,098 to 261,244
_5512
Pf1N1B4_5513 - hypothetical protein, at 261,320 to 261,517
_5513
Pf1N1B4_5514 - RecA protein, at 261,587 to 262,636
_5514
Position (kb) 260
261 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 259.514 kb on + strand, within Pf1N1B4_5510 at 259.571 kb on + strand at 259.571 kb on + strand at 259.575 kb on + strand at 259.575 kb on + strand at 259.575 kb on + strand at 259.575 kb on + strand at 259.575 kb on + strand at 259.575 kb on + strand at 259.576 kb on - strand at 259.576 kb on - strand at 259.617 kb on + strand at 259.707 kb on - strand at 259.729 kb on + strand at 259.730 kb on - strand at 259.730 kb on - strand at 259.730 kb on - strand at 259.730 kb on - strand at 259.733 kb on + strand at 259.733 kb on + strand at 259.733 kb on + strand at 259.734 kb on - strand at 259.734 kb on - strand at 259.734 kb on - strand at 259.734 kb on - strand at 259.758 kb on + strand at 259.758 kb on + strand at 259.758 kb on + strand at 259.797 kb on + strand at 259.855 kb on - strand at 259.919 kb on - strand at 259.960 kb on + strand at 259.960 kb on + strand at 259.961 kb on - strand at 259.984 kb on + strand at 259.984 kb on + strand at 259.985 kb on - strand at 260.018 kb on - strand at 260.099 kb on + strand at 260.100 kb on - strand at 260.100 kb on - strand at 260.165 kb on + strand at 260.166 kb on - strand at 260.166 kb on - strand at 260.252 kb on + strand at 260.253 kb on - strand at 260.383 kb on + strand at 260.384 kb on - strand at 260.408 kb on + strand at 260.413 kb on + strand at 260.413 kb on + strand at 260.413 kb on + strand at 260.413 kb on + strand at 260.414 kb on - strand at 260.414 kb on - strand at 260.414 kb on - strand at 260.414 kb on - strand at 260.414 kb on - strand at 260.414 kb on - strand at 260.414 kb on - strand at 260.414 kb on - strand at 260.476 kb on - strand at 260.476 kb on - strand at 260.476 kb on - strand at 260.499 kb on + strand at 260.499 kb on + strand at 260.500 kb on - strand at 260.510 kb on + strand, within Pf1N1B4_5511 at 260.511 kb on - strand, within Pf1N1B4_5511 at 260.511 kb on - strand, within Pf1N1B4_5511 at 260.511 kb on - strand, within Pf1N1B4_5511 at 260.511 kb on - strand, within Pf1N1B4_5511 at 260.575 kb on - strand, within Pf1N1B4_5511 at 260.639 kb on + strand, within Pf1N1B4_5511 at 260.639 kb on + strand, within Pf1N1B4_5511 at 260.640 kb on - strand, within Pf1N1B4_5511 at 260.640 kb on - strand, within Pf1N1B4_5511 at 260.691 kb on - strand, within Pf1N1B4_5511 at 260.786 kb on + strand at 260.786 kb on + strand at 260.787 kb on - strand at 260.787 kb on - strand at 260.787 kb on - strand at 260.787 kb on - strand at 260.790 kb on + strand at 260.920 kb on + strand at 260.920 kb on + strand at 261.018 kb on + strand at 261.018 kb on + strand at 261.164 kb on - strand, within Pf1N1B4_5512 at 261.164 kb on - strand, within Pf1N1B4_5512 at 261.166 kb on - strand, within Pf1N1B4_5512 at 261.222 kb on - strand, within Pf1N1B4_5512 at 261.260 kb on + strand at 261.260 kb on + strand at 261.261 kb on - strand at 261.261 kb on - strand at 261.265 kb on + strand at 261.265 kb on + strand at 261.266 kb on - strand at 261.266 kb on - strand at 261.287 kb on - strand at 261.287 kb on - strand at 261.296 kb on - strand at 261.316 kb on + strand at 261.316 kb on + strand at 261.317 kb on - strand at 261.317 kb on - strand at 261.321 kb on + strand at 261.321 kb on + strand at 261.321 kb on + strand at 261.322 kb on - strand at 261.322 kb on - strand at 261.322 kb on - strand at 261.322 kb on - strand at 261.458 kb on + strand, within Pf1N1B4_5513 at 261.458 kb on + strand, within Pf1N1B4_5513 at 261.459 kb on - strand, within Pf1N1B4_5513 at 261.459 kb on - strand, within Pf1N1B4_5513 at 261.459 kb on - strand, within Pf1N1B4_5513 at 261.468 kb on + strand, within Pf1N1B4_5513 at 261.534 kb on + strand at 261.535 kb on - strand at 261.550 kb on - strand at 261.555 kb on - strand at 261.668 kb on - strand at 261.712 kb on - strand, within Pf1N1B4_5514 at 261.712 kb on - strand, within Pf1N1B4_5514 at 261.712 kb on - strand, within Pf1N1B4_5514 at 261.712 kb on - strand, within Pf1N1B4_5514 at 261.712 kb on - strand, within Pf1N1B4_5514 at 261.712 kb on - strand, within Pf1N1B4_5514 at 261.712 kb on - strand, within Pf1N1B4_5514
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1 remove 259,514 + Pf1N1B4_5510 0.56 -0.0 259,571 + -0.0 259,571 + -0.0 259,575 + -0.0 259,575 + -0.0 259,575 + -2.0 259,575 + -1.6 259,575 + -0.6 259,575 + -0.2 259,576 - -3.2 259,576 - -0.0 259,617 + -0.0 259,707 - -0.0 259,729 + -0.0 259,730 - -2.4 259,730 - -0.0 259,730 - -0.0 259,730 - -0.0 259,733 + -0.0 259,733 + -0.0 259,733 + -2.9 259,734 - -1.9 259,734 - -0.0 259,734 - -0.0 259,734 - -1.5 259,758 + -0.0 259,758 + -0.0 259,758 + -0.0 259,797 + -0.0 259,855 - -2.6 259,919 - -2.4 259,960 + -1.0 259,960 + -0.0 259,961 - -0.0 259,984 + -2.2 259,984 + -0.0 259,985 - -0.0 260,018 - -0.0 260,099 + -2.9 260,100 - -0.0 260,100 - -3.2 260,165 + -1.9 260,166 - -1.4 260,166 - -1.3 260,252 + -0.0 260,253 - -0.0 260,383 + -0.0 260,384 - -0.0 260,408 + -0.0 260,413 + -0.0 260,413 + -0.0 260,413 + -0.0 260,413 + -0.0 260,414 - -1.3 260,414 - -2.0 260,414 - -1.7 260,414 - -2.2 260,414 - -1.2 260,414 - -1.9 260,414 - -0.0 260,414 - -0.0 260,476 - -0.0 260,476 - -0.0 260,476 - -1.9 260,499 + -0.0 260,499 + -0.0 260,500 - -2.5 260,510 + Pf1N1B4_5511 0.13 -0.0 260,511 - Pf1N1B4_5511 0.13 -0.0 260,511 - Pf1N1B4_5511 0.13 -0.0 260,511 - Pf1N1B4_5511 0.13 -0.0 260,511 - Pf1N1B4_5511 0.13 -0.0 260,575 - Pf1N1B4_5511 0.36 -3.9 260,639 + Pf1N1B4_5511 0.59 -0.0 260,639 + Pf1N1B4_5511 0.59 -3.6 260,640 - Pf1N1B4_5511 0.59 -0.0 260,640 - Pf1N1B4_5511 0.59 -0.0 260,691 - Pf1N1B4_5511 0.77 -0.0 260,786 + -1.8 260,786 + -2.7 260,787 - -0.0 260,787 - -2.2 260,787 - -0.6 260,787 - -0.0 260,790 + -0.5 260,920 + -1.0 260,920 + -0.0 261,018 + -1.2 261,018 + -1.2 261,164 - Pf1N1B4_5512 0.45 -0.0 261,164 - Pf1N1B4_5512 0.45 -2.1 261,166 - Pf1N1B4_5512 0.46 -0.0 261,222 - Pf1N1B4_5512 0.84 -1.0 261,260 + -1.5 261,260 + -0.0 261,261 - -0.0 261,261 - -0.0 261,265 + -0.0 261,265 + -1.2 261,266 - -0.0 261,266 - -0.0 261,287 - -1.7 261,287 - -0.0 261,296 - -1.0 261,316 + -0.0 261,316 + -0.0 261,317 - -0.0 261,317 - -0.0 261,321 + -0.0 261,321 + -0.0 261,321 + -0.0 261,322 - -0.0 261,322 - -2.1 261,322 - -2.8 261,322 - -0.0 261,458 + Pf1N1B4_5513 0.70 -1.5 261,458 + Pf1N1B4_5513 0.70 -0.0 261,459 - Pf1N1B4_5513 0.70 -0.0 261,459 - Pf1N1B4_5513 0.70 -2.2 261,459 - Pf1N1B4_5513 0.70 -0.0 261,468 + Pf1N1B4_5513 0.75 -2.0 261,534 + -0.0 261,535 - -0.0 261,550 - -0.0 261,555 - -0.0 261,668 - -0.0 261,712 - Pf1N1B4_5514 0.12 -0.0 261,712 - Pf1N1B4_5514 0.12 -0.0 261,712 - Pf1N1B4_5514 0.12 -2.2 261,712 - Pf1N1B4_5514 0.12 -1.8 261,712 - Pf1N1B4_5514 0.12 -0.0 261,712 - Pf1N1B4_5514 0.12 -0.0 261,712 - Pf1N1B4_5514 0.12 +0.6
Or see this region's nucleotide sequence