Experiment: Nitrate reduction, replicate A t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5056 and Pf1N1B4_5057 are separated by 68 nucleotides Pf1N1B4_5057 and Pf1N1B4_5058 overlap by 4 nucleotides
Pf1N1B4_5056 - Uncharacterized glutathione S-transferase-like protein, at 5,600,320 to 5,601,006
_5056
Pf1N1B4_5057 - mandelate racemase family protein Pfl_3283, at 5,601,075 to 5,602,349
_5057
Pf1N1B4_5058 - D-galactarate permease, at 5,602,346 to 5,603,743
_5058
Position (kb)
5601
5602
5603 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5600.195 kb on + strand at 5600.195 kb on + strand at 5600.242 kb on + strand at 5600.335 kb on + strand at 5600.338 kb on + strand at 5600.339 kb on - strand at 5600.369 kb on + strand at 5600.539 kb on + strand, within Pf1N1B4_5056 at 5600.635 kb on + strand, within Pf1N1B4_5056 at 5600.639 kb on + strand, within Pf1N1B4_5056 at 5600.639 kb on + strand, within Pf1N1B4_5056 at 5600.640 kb on - strand at 5600.640 kb on - strand, within Pf1N1B4_5056 at 5600.728 kb on + strand, within Pf1N1B4_5056 at 5600.821 kb on + strand, within Pf1N1B4_5056 at 5600.821 kb on + strand, within Pf1N1B4_5056 at 5600.826 kb on + strand at 5600.872 kb on + strand, within Pf1N1B4_5056 at 5601.163 kb on + strand at 5601.164 kb on - strand at 5601.619 kb on + strand, within Pf1N1B4_5057 at 5601.620 kb on - strand at 5601.871 kb on + strand, within Pf1N1B4_5057 at 5601.872 kb on - strand, within Pf1N1B4_5057 at 5601.872 kb on - strand, within Pf1N1B4_5057 at 5602.836 kb on + strand, within Pf1N1B4_5058 at 5602.836 kb on + strand, within Pf1N1B4_5058 at 5602.837 kb on - strand at 5602.947 kb on + strand, within Pf1N1B4_5058 at 5602.948 kb on - strand at 5603.340 kb on + strand at 5603.341 kb on - strand, within Pf1N1B4_5058 at 5603.343 kb on + strand, within Pf1N1B4_5058 at 5603.344 kb on - strand, within Pf1N1B4_5058
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1 remove 5,600,195 + +0.0 5,600,195 + +0.0 5,600,242 + -1.9 5,600,335 + -1.6 5,600,338 + +0.0 5,600,339 - -1.6 5,600,369 + -1.8 5,600,539 + Pf1N1B4_5056 0.32 +0.0 5,600,635 + Pf1N1B4_5056 0.46 -2.2 5,600,639 + Pf1N1B4_5056 0.46 -1.7 5,600,639 + Pf1N1B4_5056 0.46 -1.9 5,600,640 - -2.3 5,600,640 - Pf1N1B4_5056 0.47 +0.0 5,600,728 + Pf1N1B4_5056 0.59 -2.6 5,600,821 + Pf1N1B4_5056 0.73 -1.9 5,600,821 + Pf1N1B4_5056 0.73 +0.0 5,600,826 + +0.0 5,600,872 + Pf1N1B4_5056 0.80 +0.0 5,601,163 + -2.0 5,601,164 - +0.0 5,601,619 + Pf1N1B4_5057 0.43 -2.6 5,601,620 - -1.1 5,601,871 + Pf1N1B4_5057 0.62 +0.0 5,601,872 - Pf1N1B4_5057 0.63 -2.2 5,601,872 - Pf1N1B4_5057 0.63 -2.8 5,602,836 + Pf1N1B4_5058 0.35 -2.3 5,602,836 + Pf1N1B4_5058 0.35 +0.0 5,602,837 - -1.4 5,602,947 + Pf1N1B4_5058 0.43 -1.1 5,602,948 - -1.5 5,603,340 + -1.5 5,603,341 - Pf1N1B4_5058 0.71 +0.0 5,603,343 + Pf1N1B4_5058 0.71 -1.1 5,603,344 - Pf1N1B4_5058 0.71 +0.0
Or see this region's nucleotide sequence