Experiment: Nitrate reduction, replicate A t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5027 and Pf1N1B4_5028 overlap by 22 nucleotides Pf1N1B4_5028 and Pf1N1B4_5029 are separated by 216 nucleotides Pf1N1B4_5029 and Pf1N1B4_5030 are separated by 0 nucleotides Pf1N1B4_5030 and Pf1N1B4_5031 are separated by 3 nucleotides
Pf1N1B4_5027 - Enoyl-[acyl-carrier-protein] reductase [FMN] (EC 1.3.1.9), at 5,574,195 to 5,574,779
_5027
Pf1N1B4_5028 - Enoyl-[acyl-carrier-protein] reductase [FMN] (EC 1.3.1.9), at 5,574,758 to 5,575,258
_5028
Pf1N1B4_5029 - Molybdenum ABC transporter, periplasmic molybdenum-binding protein ModA (TC 3.A.1.8.1), at 5,575,475 to 5,576,233
_5029
Pf1N1B4_5030 - Molybdenum transport system permease protein ModB (TC 3.A.1.8.1), at 5,576,234 to 5,576,914
_5030
Pf1N1B4_5031 - Molybdenum transport ATP-binding protein ModC (TC 3.A.1.8.1), at 5,576,918 to 5,577,997
_5031
Position (kb)
5575
5576
5577 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 5574.483 kb on + strand, within Pf1N1B4_5027 at 5574.484 kb on - strand, within Pf1N1B4_5027 at 5574.789 kb on + strand at 5574.926 kb on + strand, within Pf1N1B4_5028 at 5574.926 kb on + strand, within Pf1N1B4_5028 at 5574.926 kb on + strand, within Pf1N1B4_5028 at 5574.926 kb on + strand, within Pf1N1B4_5028 at 5574.926 kb on + strand, within Pf1N1B4_5028 at 5574.927 kb on - strand, within Pf1N1B4_5028 at 5575.256 kb on + strand at 5575.256 kb on + strand at 5575.412 kb on + strand at 5575.429 kb on - strand at 5575.431 kb on - strand at 5575.431 kb on - strand at 5575.778 kb on + strand, within Pf1N1B4_5029 at 5575.820 kb on + strand, within Pf1N1B4_5029 at 5575.820 kb on + strand, within Pf1N1B4_5029 at 5575.820 kb on + strand at 5575.821 kb on - strand, within Pf1N1B4_5029 at 5576.049 kb on - strand, within Pf1N1B4_5029 at 5576.138 kb on - strand, within Pf1N1B4_5029 at 5576.177 kb on + strand at 5576.231 kb on + strand at 5576.232 kb on - strand at 5576.426 kb on + strand, within Pf1N1B4_5030 at 5576.427 kb on - strand, within Pf1N1B4_5030 at 5576.427 kb on - strand, within Pf1N1B4_5030 at 5576.427 kb on - strand at 5576.537 kb on + strand, within Pf1N1B4_5030 at 5576.730 kb on + strand, within Pf1N1B4_5030 at 5576.731 kb on - strand, within Pf1N1B4_5030 at 5576.733 kb on + strand, within Pf1N1B4_5030 at 5576.733 kb on + strand, within Pf1N1B4_5030 at 5576.734 kb on - strand at 5576.789 kb on + strand, within Pf1N1B4_5030 at 5576.789 kb on + strand, within Pf1N1B4_5030 at 5576.790 kb on - strand, within Pf1N1B4_5030 at 5576.790 kb on - strand, within Pf1N1B4_5030 at 5576.890 kb on + strand at 5576.946 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1 remove 5,574,483 + Pf1N1B4_5027 0.49 -2.0 5,574,484 - Pf1N1B4_5027 0.49 +0.0 5,574,789 + +0.0 5,574,926 + Pf1N1B4_5028 0.34 -1.3 5,574,926 + Pf1N1B4_5028 0.34 -1.5 5,574,926 + Pf1N1B4_5028 0.34 +0.0 5,574,926 + Pf1N1B4_5028 0.34 +0.0 5,574,926 + Pf1N1B4_5028 0.34 -1.5 5,574,927 - Pf1N1B4_5028 0.34 -1.7 5,575,256 + +0.0 5,575,256 + -1.4 5,575,412 + +0.0 5,575,429 - +0.0 5,575,431 - +0.0 5,575,431 - +0.0 5,575,778 + Pf1N1B4_5029 0.40 +0.0 5,575,820 + Pf1N1B4_5029 0.45 -3.3 5,575,820 + Pf1N1B4_5029 0.45 -2.6 5,575,820 + +0.0 5,575,821 - Pf1N1B4_5029 0.46 -3.1 5,576,049 - Pf1N1B4_5029 0.76 +0.0 5,576,138 - Pf1N1B4_5029 0.87 +0.0 5,576,177 + +0.0 5,576,231 + -2.1 5,576,232 - -2.6 5,576,426 + Pf1N1B4_5030 0.28 +0.0 5,576,427 - Pf1N1B4_5030 0.28 -1.7 5,576,427 - Pf1N1B4_5030 0.28 +0.0 5,576,427 - +0.0 5,576,537 + Pf1N1B4_5030 0.44 -4.0 5,576,730 + Pf1N1B4_5030 0.73 +0.0 5,576,731 - Pf1N1B4_5030 0.73 -3.3 5,576,733 + Pf1N1B4_5030 0.73 +0.0 5,576,733 + Pf1N1B4_5030 0.73 -1.7 5,576,734 - -4.0 5,576,789 + Pf1N1B4_5030 0.81 -4.3 5,576,789 + Pf1N1B4_5030 0.81 -3.1 5,576,790 - Pf1N1B4_5030 0.82 -3.2 5,576,790 - Pf1N1B4_5030 0.82 -4.6 5,576,890 + +0.0 5,576,946 - -4.0
Or see this region's nucleotide sequence