Experiment: Nitrate reduction, replicate A t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4750 and Pf1N1B4_4751 are separated by 161 nucleotides
Pf1N1B4_4750 - Fe(2+)/alpha-ketoglutarate-dependent dioxygenase LpxO, at 5,247,959 to 5,248,795
_4750
Pf1N1B4_4751 - Ku domain protein, at 5,248,957 to 5,249,817
_4751
Position (kb) 5247
5248
5249 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 5246.982 kb on - strand at 5247.072 kb on + strand at 5247.073 kb on - strand at 5247.073 kb on - strand at 5247.073 kb on - strand at 5247.085 kb on + strand at 5247.086 kb on - strand at 5247.086 kb on - strand at 5247.210 kb on + strand at 5247.211 kb on - strand at 5247.320 kb on - strand at 5247.320 kb on - strand at 5247.320 kb on - strand at 5247.320 kb on - strand at 5247.326 kb on + strand at 5247.327 kb on - strand at 5247.334 kb on - strand at 5247.334 kb on - strand at 5247.334 kb on - strand at 5247.363 kb on - strand at 5247.396 kb on - strand at 5247.421 kb on - strand at 5247.421 kb on - strand at 5247.421 kb on - strand at 5247.519 kb on - strand at 5247.519 kb on - strand at 5247.519 kb on - strand at 5247.519 kb on - strand at 5247.519 kb on - strand at 5247.519 kb on - strand at 5247.519 kb on - strand at 5247.519 kb on - strand at 5247.519 kb on - strand at 5247.559 kb on - strand at 5247.559 kb on - strand at 5247.571 kb on - strand at 5247.571 kb on - strand at 5247.571 kb on - strand at 5247.592 kb on - strand at 5247.592 kb on - strand at 5247.592 kb on - strand at 5247.592 kb on - strand at 5247.611 kb on - strand at 5247.611 kb on - strand at 5247.611 kb on - strand at 5247.611 kb on - strand at 5247.668 kb on - strand at 5247.668 kb on - strand at 5247.668 kb on - strand at 5247.668 kb on - strand at 5247.668 kb on - strand at 5247.668 kb on - strand at 5247.668 kb on - strand at 5247.671 kb on + strand at 5247.672 kb on - strand at 5247.672 kb on - strand at 5247.744 kb on - strand at 5247.744 kb on - strand at 5247.747 kb on - strand at 5247.747 kb on - strand at 5247.747 kb on - strand at 5247.747 kb on - strand at 5247.747 kb on - strand at 5247.747 kb on - strand at 5247.747 kb on - strand at 5247.747 kb on - strand at 5247.747 kb on - strand at 5247.779 kb on + strand at 5247.780 kb on - strand at 5247.780 kb on - strand at 5247.780 kb on - strand at 5247.780 kb on - strand at 5247.780 kb on - strand at 5247.780 kb on - strand at 5247.780 kb on - strand at 5247.780 kb on - strand at 5247.780 kb on - strand at 5247.780 kb on - strand at 5247.780 kb on - strand at 5247.780 kb on - strand at 5247.780 kb on - strand at 5247.780 kb on - strand at 5247.783 kb on - strand at 5247.783 kb on - strand at 5247.794 kb on - strand at 5247.800 kb on - strand at 5247.905 kb on - strand at 5247.905 kb on - strand at 5247.920 kb on + strand at 5247.920 kb on + strand at 5247.921 kb on - strand at 5247.921 kb on - strand at 5247.938 kb on + strand at 5247.939 kb on - strand at 5247.973 kb on - strand at 5247.985 kb on - strand at 5248.028 kb on + strand at 5248.029 kb on - strand at 5248.029 kb on - strand at 5248.059 kb on - strand, within Pf1N1B4_4750 at 5248.173 kb on - strand, within Pf1N1B4_4750 at 5248.382 kb on + strand, within Pf1N1B4_4750 at 5248.427 kb on + strand, within Pf1N1B4_4750 at 5248.427 kb on + strand, within Pf1N1B4_4750 at 5248.427 kb on + strand, within Pf1N1B4_4750 at 5248.427 kb on + strand, within Pf1N1B4_4750 at 5248.427 kb on + strand at 5248.427 kb on + strand, within Pf1N1B4_4750 at 5248.427 kb on + strand, within Pf1N1B4_4750 at 5248.427 kb on + strand, within Pf1N1B4_4750 at 5248.427 kb on + strand, within Pf1N1B4_4750 at 5248.427 kb on + strand, within Pf1N1B4_4750 at 5248.427 kb on + strand, within Pf1N1B4_4750 at 5248.428 kb on - strand, within Pf1N1B4_4750 at 5248.428 kb on - strand, within Pf1N1B4_4750 at 5248.428 kb on - strand, within Pf1N1B4_4750 at 5248.428 kb on - strand, within Pf1N1B4_4750 at 5248.428 kb on - strand at 5248.428 kb on - strand, within Pf1N1B4_4750 at 5248.428 kb on - strand, within Pf1N1B4_4750 at 5248.428 kb on - strand, within Pf1N1B4_4750 at 5248.428 kb on - strand, within Pf1N1B4_4750 at 5248.428 kb on - strand, within Pf1N1B4_4750 at 5248.430 kb on - strand, within Pf1N1B4_4750 at 5248.451 kb on + strand, within Pf1N1B4_4750 at 5248.451 kb on + strand, within Pf1N1B4_4750 at 5248.451 kb on + strand, within Pf1N1B4_4750 at 5248.452 kb on - strand, within Pf1N1B4_4750 at 5248.502 kb on + strand, within Pf1N1B4_4750 at 5248.503 kb on - strand, within Pf1N1B4_4750 at 5248.568 kb on + strand, within Pf1N1B4_4750 at 5248.569 kb on - strand at 5248.660 kb on + strand, within Pf1N1B4_4750 at 5248.660 kb on + strand, within Pf1N1B4_4750 at 5248.661 kb on - strand, within Pf1N1B4_4750 at 5248.661 kb on - strand, within Pf1N1B4_4750 at 5248.685 kb on + strand, within Pf1N1B4_4750 at 5248.686 kb on - strand, within Pf1N1B4_4750 at 5248.686 kb on - strand, within Pf1N1B4_4750 at 5248.724 kb on - strand at 5248.737 kb on - strand at 5248.739 kb on + strand at 5248.739 kb on + strand at 5248.740 kb on - strand at 5248.860 kb on - strand at 5248.860 kb on - strand at 5248.919 kb on - strand at 5248.936 kb on + strand at 5248.936 kb on + strand at 5248.946 kb on - strand at 5248.946 kb on - strand at 5248.946 kb on - strand at 5249.071 kb on - strand, within Pf1N1B4_4751 at 5249.086 kb on - strand at 5249.450 kb on + strand at 5249.451 kb on - strand, within Pf1N1B4_4751 at 5249.451 kb on - strand at 5249.483 kb on + strand, within Pf1N1B4_4751 at 5249.484 kb on - strand, within Pf1N1B4_4751 at 5249.484 kb on - strand, within Pf1N1B4_4751 at 5249.487 kb on - strand, within Pf1N1B4_4751 at 5249.487 kb on - strand, within Pf1N1B4_4751 at 5249.603 kb on + strand, within Pf1N1B4_4751 at 5249.604 kb on - strand at 5249.619 kb on - strand, within Pf1N1B4_4751
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1 remove 5,246,982 - +0.0 5,247,072 + -2.1 5,247,073 - -0.3 5,247,073 - -1.5 5,247,073 - -1.5 5,247,085 + -1.4 5,247,086 - +0.0 5,247,086 - -1.8 5,247,210 + -2.7 5,247,211 - -2.9 5,247,320 - -1.0 5,247,320 - +0.0 5,247,320 - +0.0 5,247,320 - -1.0 5,247,326 + -1.3 5,247,327 - +0.0 5,247,334 - -0.3 5,247,334 - +0.0 5,247,334 - -1.8 5,247,363 - -1.6 5,247,396 - -1.7 5,247,421 - +0.0 5,247,421 - -0.9 5,247,421 - -1.2 5,247,519 - +0.0 5,247,519 - -1.7 5,247,519 - -1.8 5,247,519 - +0.0 5,247,519 - -0.3 5,247,519 - -2.0 5,247,519 - -2.0 5,247,519 - +0.0 5,247,519 - +0.0 5,247,559 - -1.0 5,247,559 - -1.6 5,247,571 - +0.0 5,247,571 - +0.0 5,247,571 - -1.4 5,247,592 - -0.6 5,247,592 - -2.1 5,247,592 - -1.2 5,247,592 - +0.0 5,247,611 - -1.5 5,247,611 - -1.8 5,247,611 - -1.1 5,247,611 - +1.1 5,247,668 - -1.0 5,247,668 - -1.4 5,247,668 - -1.2 5,247,668 - +0.0 5,247,668 - -1.3 5,247,668 - +0.0 5,247,668 - +0.0 5,247,671 + +0.0 5,247,672 - +0.0 5,247,672 - -1.6 5,247,744 - -2.3 5,247,744 - -0.7 5,247,747 - +0.0 5,247,747 - -2.3 5,247,747 - +0.0 5,247,747 - -1.0 5,247,747 - +0.0 5,247,747 - -2.2 5,247,747 - -0.9 5,247,747 - +0.0 5,247,747 - +0.0 5,247,779 + +0.0 5,247,780 - -1.8 5,247,780 - -2.6 5,247,780 - +0.0 5,247,780 - +0.0 5,247,780 - -0.9 5,247,780 - +0.0 5,247,780 - -1.2 5,247,780 - +0.0 5,247,780 - +0.0 5,247,780 - +0.0 5,247,780 - +0.0 5,247,780 - +0.0 5,247,780 - -0.2 5,247,780 - +0.0 5,247,783 - +0.0 5,247,783 - -1.3 5,247,794 - -0.9 5,247,800 - +0.0 5,247,905 - -1.4 5,247,905 - +0.0 5,247,920 + +0.0 5,247,920 + -1.0 5,247,921 - +0.0 5,247,921 - -1.0 5,247,938 + -1.3 5,247,939 - -1.8 5,247,973 - +0.0 5,247,985 - -2.4 5,248,028 + -2.3 5,248,029 - +0.0 5,248,029 - +0.0 5,248,059 - Pf1N1B4_4750 0.12 -0.5 5,248,173 - Pf1N1B4_4750 0.26 -1.3 5,248,382 + Pf1N1B4_4750 0.51 -0.7 5,248,427 + Pf1N1B4_4750 0.56 -2.0 5,248,427 + Pf1N1B4_4750 0.56 -0.8 5,248,427 + Pf1N1B4_4750 0.56 -1.2 5,248,427 + Pf1N1B4_4750 0.56 -1.0 5,248,427 + -1.0 5,248,427 + Pf1N1B4_4750 0.56 +0.0 5,248,427 + Pf1N1B4_4750 0.56 +0.0 5,248,427 + Pf1N1B4_4750 0.56 +0.0 5,248,427 + Pf1N1B4_4750 0.56 +0.0 5,248,427 + Pf1N1B4_4750 0.56 +0.0 5,248,427 + Pf1N1B4_4750 0.56 +0.0 5,248,428 - Pf1N1B4_4750 0.56 -1.0 5,248,428 - Pf1N1B4_4750 0.56 +0.0 5,248,428 - Pf1N1B4_4750 0.56 +0.0 5,248,428 - Pf1N1B4_4750 0.56 +0.0 5,248,428 - +0.0 5,248,428 - Pf1N1B4_4750 0.56 -1.1 5,248,428 - Pf1N1B4_4750 0.56 -1.9 5,248,428 - Pf1N1B4_4750 0.56 -1.8 5,248,428 - Pf1N1B4_4750 0.56 +0.0 5,248,428 - Pf1N1B4_4750 0.56 -0.3 5,248,430 - Pf1N1B4_4750 0.56 -1.6 5,248,451 + Pf1N1B4_4750 0.59 +0.0 5,248,451 + Pf1N1B4_4750 0.59 +0.0 5,248,451 + Pf1N1B4_4750 0.59 +0.0 5,248,452 - Pf1N1B4_4750 0.59 -1.7 5,248,502 + Pf1N1B4_4750 0.65 -0.9 5,248,503 - Pf1N1B4_4750 0.65 -1.1 5,248,568 + Pf1N1B4_4750 0.73 +0.0 5,248,569 - -0.6 5,248,660 + Pf1N1B4_4750 0.84 -0.8 5,248,660 + Pf1N1B4_4750 0.84 -1.9 5,248,661 - Pf1N1B4_4750 0.84 +0.0 5,248,661 - Pf1N1B4_4750 0.84 -1.6 5,248,685 + Pf1N1B4_4750 0.87 -1.9 5,248,686 - Pf1N1B4_4750 0.87 +0.0 5,248,686 - Pf1N1B4_4750 0.87 -1.4 5,248,724 - +0.0 5,248,737 - -1.0 5,248,739 + +0.0 5,248,739 + +0.0 5,248,740 - +0.0 5,248,860 - +0.0 5,248,860 - -0.7 5,248,919 - +0.0 5,248,936 + -2.0 5,248,936 + +0.0 5,248,946 - +0.0 5,248,946 - +0.0 5,248,946 - -2.7 5,249,071 - Pf1N1B4_4751 0.13 -1.9 5,249,086 - -0.7 5,249,450 + +0.0 5,249,451 - Pf1N1B4_4751 0.57 -4.6 5,249,451 - -1.0 5,249,483 + Pf1N1B4_4751 0.61 +0.0 5,249,484 - Pf1N1B4_4751 0.61 +0.0 5,249,484 - Pf1N1B4_4751 0.61 +0.0 5,249,487 - Pf1N1B4_4751 0.62 -1.6 5,249,487 - Pf1N1B4_4751 0.62 +0.0 5,249,603 + Pf1N1B4_4751 0.75 -2.2 5,249,604 - +0.0 5,249,619 - Pf1N1B4_4751 0.77 +0.0
Or see this region's nucleotide sequence