Experiment: Nitrate reduction, replicate A t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4747 and Pf1N1B4_4748 are separated by 121 nucleotides Pf1N1B4_4748 and Pf1N1B4_4749 are separated by 246 nucleotides
Pf1N1B4_4747 - Probable lipoprotein signal peptide, at 5,242,185 to 5,243,222
_4747
Pf1N1B4_4748 - transporter, putative, at 5,243,344 to 5,244,531
_4748
Pf1N1B4_4749 - ABC transporter ATP-binding protein uup, at 5,244,778 to 5,246,343
_4749
Position (kb) 5243
5244
5245 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5242.901 kb on + strand, within Pf1N1B4_4747 at 5242.902 kb on - strand at 5243.047 kb on + strand, within Pf1N1B4_4747 at 5243.048 kb on - strand at 5243.220 kb on + strand at 5243.281 kb on + strand at 5243.282 kb on - strand at 5243.282 kb on - strand at 5243.288 kb on - strand at 5243.288 kb on - strand at 5243.288 kb on - strand at 5243.288 kb on - strand at 5243.346 kb on - strand at 5243.346 kb on - strand at 5243.502 kb on - strand, within Pf1N1B4_4748 at 5243.772 kb on - strand, within Pf1N1B4_4748 at 5243.801 kb on + strand, within Pf1N1B4_4748 at 5243.801 kb on + strand, within Pf1N1B4_4748 at 5243.801 kb on + strand, within Pf1N1B4_4748 at 5243.802 kb on - strand, within Pf1N1B4_4748 at 5243.802 kb on - strand, within Pf1N1B4_4748 at 5243.802 kb on - strand, within Pf1N1B4_4748 at 5243.805 kb on - strand, within Pf1N1B4_4748 at 5243.814 kb on + strand, within Pf1N1B4_4748 at 5243.815 kb on - strand, within Pf1N1B4_4748 at 5243.815 kb on - strand, within Pf1N1B4_4748 at 5243.820 kb on + strand at 5243.821 kb on - strand, within Pf1N1B4_4748 at 5244.461 kb on + strand at 5244.461 kb on + strand at 5244.462 kb on - strand at 5244.462 kb on - strand at 5244.462 kb on - strand at 5244.462 kb on - strand at 5244.587 kb on + strand at 5244.780 kb on - strand at 5244.780 kb on - strand at 5244.780 kb on - strand at 5244.780 kb on - strand at 5244.807 kb on - strand at 5244.815 kb on + strand at 5244.815 kb on + strand at 5244.816 kb on - strand at 5245.209 kb on - strand, within Pf1N1B4_4749 at 5245.212 kb on - strand, within Pf1N1B4_4749
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1 remove 5,242,901 + Pf1N1B4_4747 0.69 +0.0 5,242,902 - -2.8 5,243,047 + Pf1N1B4_4747 0.83 +0.0 5,243,048 - +0.0 5,243,220 + -1.6 5,243,281 + +0.0 5,243,282 - +0.0 5,243,282 - -1.6 5,243,288 - +0.3 5,243,288 - +0.0 5,243,288 - +0.0 5,243,288 - -1.7 5,243,346 - -1.0 5,243,346 - +0.0 5,243,502 - Pf1N1B4_4748 0.13 -2.1 5,243,772 - Pf1N1B4_4748 0.36 -0.6 5,243,801 + Pf1N1B4_4748 0.38 -0.5 5,243,801 + Pf1N1B4_4748 0.38 +0.0 5,243,801 + Pf1N1B4_4748 0.38 -1.9 5,243,802 - Pf1N1B4_4748 0.39 +0.0 5,243,802 - Pf1N1B4_4748 0.39 +0.0 5,243,802 - Pf1N1B4_4748 0.39 +0.0 5,243,805 - Pf1N1B4_4748 0.39 +0.0 5,243,814 + Pf1N1B4_4748 0.40 +0.0 5,243,815 - Pf1N1B4_4748 0.40 +0.0 5,243,815 - Pf1N1B4_4748 0.40 +0.0 5,243,820 + -1.0 5,243,821 - Pf1N1B4_4748 0.40 +0.0 5,244,461 + +0.0 5,244,461 + -3.2 5,244,462 - +0.0 5,244,462 - +0.0 5,244,462 - +0.0 5,244,462 - -2.8 5,244,587 + +0.0 5,244,780 - -1.8 5,244,780 - -2.1 5,244,780 - +0.0 5,244,780 - +0.2 5,244,807 - -1.5 5,244,815 + +0.0 5,244,815 + -2.5 5,244,816 - +0.0 5,245,209 - Pf1N1B4_4749 0.28 +0.0 5,245,212 - Pf1N1B4_4749 0.28 -1.6
Or see this region's nucleotide sequence