Experiment: Nitrate reduction, replicate A t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4580 and Pf1N1B4_4581 are separated by 4 nucleotides Pf1N1B4_4581 and Pf1N1B4_4582 are separated by 176 nucleotides Pf1N1B4_4582 and Pf1N1B4_4583 are separated by 142 nucleotides Pf1N1B4_4583 and Pf1N1B4_4584 are separated by 24 nucleotides
Pf1N1B4_4580 - Fe2+-dicitrate sensor, membrane component, at 5,051,945 to 5,052,931
_4580
Pf1N1B4_4581 - RNA polymerase sigma-70 factor, ECF subfamily, at 5,052,936 to 5,053,460
_4581
Pf1N1B4_4582 - hypothetical protein, at 5,053,637 to 5,053,819
_4582
Pf1N1B4_4583 - Isoquinoline 1-oxidoreductase alpha subunit (EC 1.3.99.16), at 5,053,962 to 5,054,417
_4583
Pf1N1B4_4584 - Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16), at 5,054,442 to 5,056,610
_4584
Position (kb) 5053
5054 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5052.714 kb on - strand, within Pf1N1B4_4580 at 5052.774 kb on + strand, within Pf1N1B4_4580 at 5052.845 kb on + strand at 5052.871 kb on - strand at 5053.003 kb on + strand, within Pf1N1B4_4581 at 5053.082 kb on + strand at 5053.327 kb on - strand at 5053.421 kb on + strand at 5053.421 kb on + strand at 5053.422 kb on - strand at 5053.423 kb on + strand at 5053.598 kb on + strand at 5053.598 kb on + strand at 5053.650 kb on - strand at 5053.660 kb on + strand, within Pf1N1B4_4582 at 5053.660 kb on + strand, within Pf1N1B4_4582 at 5053.660 kb on + strand, within Pf1N1B4_4582 at 5053.774 kb on - strand, within Pf1N1B4_4582 at 5053.973 kb on + strand at 5053.973 kb on + strand at 5053.977 kb on - strand at 5054.104 kb on + strand, within Pf1N1B4_4583 at 5054.105 kb on - strand, within Pf1N1B4_4583
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1 remove 5,052,714 - Pf1N1B4_4580 0.78 -1.0 5,052,774 + Pf1N1B4_4580 0.84 +0.0 5,052,845 + +0.0 5,052,871 - +0.0 5,053,003 + Pf1N1B4_4581 0.13 +0.0 5,053,082 + +0.0 5,053,327 - +0.0 5,053,421 + +0.0 5,053,421 + -1.5 5,053,422 - +0.0 5,053,423 + +0.0 5,053,598 + +0.0 5,053,598 + -0.6 5,053,650 - +0.0 5,053,660 + Pf1N1B4_4582 0.13 +0.0 5,053,660 + Pf1N1B4_4582 0.13 +0.0 5,053,660 + Pf1N1B4_4582 0.13 -3.2 5,053,774 - Pf1N1B4_4582 0.75 +0.0 5,053,973 + +0.0 5,053,973 + +0.0 5,053,977 - -3.4 5,054,104 + Pf1N1B4_4583 0.31 -0.2 5,054,105 - Pf1N1B4_4583 0.31 +0.0
Or see this region's nucleotide sequence