Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_45

Experiment: Nitrate reduction, replicate A t1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_44 and Pf1N1B4_45 are separated by 10 nucleotidesPf1N1B4_45 and Pf1N1B4_46 are separated by 55 nucleotidesPf1N1B4_46 and Pf1N1B4_47 are separated by 3 nucleotides Pf1N1B4_44 - Multicopper oxidase, at 51,784 to 53,577 _44 Pf1N1B4_45 - Copper resistance protein B, at 53,588 to 54,478 _45 Pf1N1B4_46 - Copper resistance protein C precursor, at 54,534 to 54,917 _46 Pf1N1B4_47 - Copper resistance protein D, at 54,921 to 55,850 _47 Position (kb) 53 54 55Strain fitness (log2 ratio) -3 -2 -1 0 1at 52.624 kb on + strand, within Pf1N1B4_44at 52.765 kb on + strandat 52.765 kb on + strand, within Pf1N1B4_44at 52.765 kb on + strand, within Pf1N1B4_44at 52.766 kb on - strand, within Pf1N1B4_44at 52.766 kb on - strand, within Pf1N1B4_44at 53.177 kb on - strand, within Pf1N1B4_44at 53.512 kb on + strandat 53.531 kb on - strandat 53.689 kb on + strand, within Pf1N1B4_45at 53.816 kb on + strandat 53.861 kb on + strand, within Pf1N1B4_45at 53.861 kb on + strand, within Pf1N1B4_45at 53.861 kb on + strandat 53.861 kb on + strandat 53.861 kb on + strand, within Pf1N1B4_45at 53.861 kb on + strandat 53.862 kb on - strandat 53.862 kb on - strand, within Pf1N1B4_45at 53.864 kb on + strand, within Pf1N1B4_45at 53.864 kb on + strand, within Pf1N1B4_45at 53.864 kb on + strand, within Pf1N1B4_45at 53.864 kb on + strand, within Pf1N1B4_45at 54.137 kb on + strand, within Pf1N1B4_45at 54.138 kb on - strand, within Pf1N1B4_45at 54.210 kb on - strand, within Pf1N1B4_45at 54.365 kb on + strandat 54.365 kb on + strand, within Pf1N1B4_45at 54.365 kb on + strand, within Pf1N1B4_45at 54.365 kb on + strand, within Pf1N1B4_45at 54.365 kb on + strand, within Pf1N1B4_45at 54.365 kb on + strand, within Pf1N1B4_45at 54.365 kb on + strand, within Pf1N1B4_45at 54.366 kb on - strand, within Pf1N1B4_45at 54.366 kb on - strand, within Pf1N1B4_45at 54.366 kb on - strand, within Pf1N1B4_45at 54.393 kb on - strandat 54.410 kb on + strandat 54.411 kb on - strandat 54.485 kb on + strandat 54.486 kb on - strandat 54.514 kb on - strandat 54.843 kb on + strand, within Pf1N1B4_46at 54.843 kb on + strand, within Pf1N1B4_46at 54.844 kb on - strand, within Pf1N1B4_46at 54.844 kb on - strand, within Pf1N1B4_46at 54.844 kb on - strand, within Pf1N1B4_46at 54.844 kb on - strand, within Pf1N1B4_46at 54.899 kb on + strandat 54.899 kb on + strandat 54.899 kb on + strandat 54.899 kb on + strandat 54.960 kb on + strandat 54.960 kb on + strandat 54.960 kb on + strandat 54.960 kb on + strandat 54.960 kb on + strandat 54.960 kb on + strandat 54.960 kb on + strandat 54.961 kb on - strandat 54.961 kb on - strandat 54.961 kb on - strandat 54.961 kb on - strandat 54.961 kb on - strandat 55.006 kb on - strandat 55.006 kb on - strandat 55.014 kb on - strand, within Pf1N1B4_47at 55.276 kb on + strand, within Pf1N1B4_47at 55.277 kb on - strand, within Pf1N1B4_47at 55.341 kb on - strand, within Pf1N1B4_47

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1
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52,624 + Pf1N1B4_44 0.47 -2.1
52,765 + -1.9
52,765 + Pf1N1B4_44 0.55 +0.0
52,765 + Pf1N1B4_44 0.55 +0.0
52,766 - Pf1N1B4_44 0.55 +0.0
52,766 - Pf1N1B4_44 0.55 +0.5
53,177 - Pf1N1B4_44 0.78 +0.0
53,512 + -0.9
53,531 - -1.6
53,689 + Pf1N1B4_45 0.11 -2.4
53,816 + -2.3
53,861 + Pf1N1B4_45 0.31 -1.0
53,861 + Pf1N1B4_45 0.31 -1.3
53,861 + -2.3
53,861 + +0.1
53,861 + Pf1N1B4_45 0.31 +0.0
53,861 + -1.6
53,862 - +0.0
53,862 - Pf1N1B4_45 0.31 -1.5
53,864 + Pf1N1B4_45 0.31 -1.2
53,864 + Pf1N1B4_45 0.31 -1.3
53,864 + Pf1N1B4_45 0.31 +0.0
53,864 + Pf1N1B4_45 0.31 +0.0
54,137 + Pf1N1B4_45 0.62 -2.1
54,138 - Pf1N1B4_45 0.62 +0.0
54,210 - Pf1N1B4_45 0.70 -0.6
54,365 + -2.8
54,365 + Pf1N1B4_45 0.87 +1.0
54,365 + Pf1N1B4_45 0.87 -1.3
54,365 + Pf1N1B4_45 0.87 +0.0
54,365 + Pf1N1B4_45 0.87 +0.0
54,365 + Pf1N1B4_45 0.87 +0.0
54,365 + Pf1N1B4_45 0.87 -1.4
54,366 - Pf1N1B4_45 0.87 +0.0
54,366 - Pf1N1B4_45 0.87 -0.8
54,366 - Pf1N1B4_45 0.87 -1.7
54,393 - +0.0
54,410 + -2.3
54,411 - +0.0
54,485 + -0.7
54,486 - +0.0
54,514 - -2.8
54,843 + Pf1N1B4_46 0.80 +0.0
54,843 + Pf1N1B4_46 0.80 +0.0
54,844 - Pf1N1B4_46 0.81 -1.6
54,844 - Pf1N1B4_46 0.81 -1.5
54,844 - Pf1N1B4_46 0.81 +0.0
54,844 - Pf1N1B4_46 0.81 +0.0
54,899 + +0.0
54,899 + +0.0
54,899 + +0.0
54,899 + -1.3
54,960 + +0.0
54,960 + +0.0
54,960 + -1.0
54,960 + -2.0
54,960 + +0.0
54,960 + +0.0
54,960 + -2.6
54,961 - -3.0
54,961 - +0.0
54,961 - -2.4
54,961 - +0.0
54,961 - +0.0
55,006 - -1.8
55,006 - +0.0
55,014 - Pf1N1B4_47 0.10 +0.0
55,276 + Pf1N1B4_47 0.38 +0.0
55,277 - Pf1N1B4_47 0.38 +0.0
55,341 - Pf1N1B4_47 0.45 +0.0

Or see this region's nucleotide sequence