Experiment: Nitrate reduction, replicate A t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4431 and Pf1N1B4_4432 are separated by 61 nucleotides Pf1N1B4_4432 and Pf1N1B4_4433 are separated by 47 nucleotides Pf1N1B4_4433 and Pf1N1B4_4434 are separated by 18 nucleotides Pf1N1B4_4434 and Pf1N1B4_4435 overlap by 4 nucleotides Pf1N1B4_4435 and Pf1N1B4_4436 are separated by 229 nucleotides
Pf1N1B4_4431 - Permeases of the major facilitator superfamily, at 4,892,674 to 4,893,861
_4431
Pf1N1B4_4432 - Dienelactone hydrolase family, at 4,893,923 to 4,894,621
_4432
Pf1N1B4_4433 - FIG035246: DoxX family protein, at 4,894,669 to 4,895,184
_4433
Pf1N1B4_4434 - hypothetical protein, at 4,895,203 to 4,895,325
_4434
Pf1N1B4_4435 - Inner membrane protein, at 4,895,322 to 4,895,951
_4435
Pf1N1B4_4436 - Glutaminase (EC 3.5.1.2), at 4,896,181 to 4,897,089
_4436
Position (kb)
4894
4895
4896 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4893.761 kb on - strand at 4893.823 kb on - strand at 4893.896 kb on + strand at 4893.896 kb on + strand at 4893.897 kb on - strand at 4893.897 kb on - strand at 4893.925 kb on - strand at 4893.952 kb on + strand at 4893.952 kb on + strand at 4893.953 kb on - strand at 4893.953 kb on - strand at 4893.987 kb on + strand at 4893.987 kb on + strand at 4893.988 kb on - strand at 4894.233 kb on + strand, within Pf1N1B4_4432 at 4894.467 kb on + strand, within Pf1N1B4_4432 at 4894.467 kb on + strand, within Pf1N1B4_4432 at 4894.468 kb on - strand, within Pf1N1B4_4432 at 4894.468 kb on - strand, within Pf1N1B4_4432 at 4894.634 kb on - strand at 4894.634 kb on - strand at 4894.634 kb on - strand at 4894.637 kb on + strand at 4894.637 kb on + strand at 4894.637 kb on + strand at 4894.637 kb on + strand at 4894.637 kb on + strand at 4894.638 kb on - strand at 4894.638 kb on - strand at 4894.767 kb on - strand, within Pf1N1B4_4433 at 4894.767 kb on - strand, within Pf1N1B4_4433 at 4894.801 kb on - strand, within Pf1N1B4_4433 at 4895.178 kb on + strand at 4895.178 kb on + strand at 4895.179 kb on - strand at 4895.179 kb on - strand at 4895.429 kb on - strand at 4895.429 kb on - strand, within Pf1N1B4_4435 at 4895.429 kb on - strand, within Pf1N1B4_4435 at 4895.429 kb on - strand, within Pf1N1B4_4435 at 4895.429 kb on - strand, within Pf1N1B4_4435 at 4895.653 kb on + strand, within Pf1N1B4_4435 at 4895.659 kb on + strand, within Pf1N1B4_4435 at 4895.660 kb on - strand, within Pf1N1B4_4435 at 4895.660 kb on - strand at 4895.660 kb on - strand, within Pf1N1B4_4435 at 4895.660 kb on - strand, within Pf1N1B4_4435 at 4896.087 kb on + strand at 4896.087 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1 remove 4,893,761 - -1.6 4,893,823 - +0.0 4,893,896 + +0.0 4,893,896 + +0.0 4,893,897 - +0.0 4,893,897 - +0.0 4,893,925 - -1.4 4,893,952 + -1.2 4,893,952 + -1.5 4,893,953 - +0.0 4,893,953 - -1.3 4,893,987 + +0.0 4,893,987 + +0.0 4,893,988 - +0.0 4,894,233 + Pf1N1B4_4432 0.44 +0.0 4,894,467 + Pf1N1B4_4432 0.78 -1.5 4,894,467 + Pf1N1B4_4432 0.78 -1.1 4,894,468 - Pf1N1B4_4432 0.78 -1.3 4,894,468 - Pf1N1B4_4432 0.78 -1.4 4,894,634 - +0.0 4,894,634 - +0.0 4,894,634 - +0.0 4,894,637 + +0.0 4,894,637 + +0.0 4,894,637 + +0.0 4,894,637 + -2.0 4,894,637 + -0.8 4,894,638 - +0.0 4,894,638 - +0.0 4,894,767 - Pf1N1B4_4433 0.19 +0.0 4,894,767 - Pf1N1B4_4433 0.19 +0.0 4,894,801 - Pf1N1B4_4433 0.26 -2.8 4,895,178 + +0.0 4,895,178 + -2.4 4,895,179 - -1.9 4,895,179 - -1.8 4,895,429 - -1.1 4,895,429 - Pf1N1B4_4435 0.17 -1.9 4,895,429 - Pf1N1B4_4435 0.17 -1.2 4,895,429 - Pf1N1B4_4435 0.17 -4.1 4,895,429 - Pf1N1B4_4435 0.17 -2.2 4,895,653 + Pf1N1B4_4435 0.53 -1.2 4,895,659 + Pf1N1B4_4435 0.53 +0.0 4,895,660 - Pf1N1B4_4435 0.54 -1.6 4,895,660 - +0.0 4,895,660 - Pf1N1B4_4435 0.54 +0.0 4,895,660 - Pf1N1B4_4435 0.54 +0.0 4,896,087 + +0.0 4,896,087 + +0.0
Or see this region's nucleotide sequence