Experiment: Nitrate reduction, replicate A t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4073 and Pf1N1B4_4074 are separated by 2 nucleotides Pf1N1B4_4074 and Pf1N1B4_4075 are separated by 162 nucleotides Pf1N1B4_4075 and Pf1N1B4_4076 are separated by 113 nucleotides Pf1N1B4_4076 and Pf1N1B4_4077 are separated by 147 nucleotides Pf1N1B4_4077 and Pf1N1B4_4078 are separated by 100 nucleotides
Pf1N1B4_4073 - FIG00957702: hypothetical protein, at 4,525,254 to 4,525,583
_4073
Pf1N1B4_4074 - hypothetical protein, at 4,525,586 to 4,525,840
_4074
Pf1N1B4_4075 - hypothetical protein, at 4,526,003 to 4,526,353
_4075
Pf1N1B4_4076 - Histone acetyltransferase HPA2 and related acetyltransferases, at 4,526,467 to 4,526,895
_4076
Pf1N1B4_4077 - FIG00962160: hypothetical protein, at 4,527,043 to 4,527,480
_4077
Pf1N1B4_4078 - GDP-mannose pyrophosphatase YffH, at 4,527,581 to 4,528,174
_4078
Position (kb)
4526
4527 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 4525.594 kb on + strand at 4525.717 kb on - strand, within Pf1N1B4_4074 at 4525.818 kb on + strand at 4525.818 kb on + strand at 4525.821 kb on + strand at 4525.822 kb on - strand at 4525.931 kb on + strand at 4525.931 kb on + strand at 4525.932 kb on - strand at 4525.971 kb on + strand at 4525.971 kb on + strand at 4525.972 kb on - strand at 4526.090 kb on + strand, within Pf1N1B4_4075 at 4526.189 kb on + strand, within Pf1N1B4_4075 at 4526.190 kb on - strand, within Pf1N1B4_4075 at 4526.190 kb on - strand, within Pf1N1B4_4075 at 4526.190 kb on - strand, within Pf1N1B4_4075 at 4526.225 kb on + strand, within Pf1N1B4_4075 at 4526.225 kb on + strand, within Pf1N1B4_4075 at 4526.225 kb on + strand, within Pf1N1B4_4075 at 4526.225 kb on + strand, within Pf1N1B4_4075 at 4526.225 kb on + strand, within Pf1N1B4_4075 at 4526.225 kb on + strand, within Pf1N1B4_4075 at 4526.225 kb on + strand at 4526.226 kb on - strand, within Pf1N1B4_4075 at 4526.251 kb on - strand, within Pf1N1B4_4075 at 4526.288 kb on + strand, within Pf1N1B4_4075 at 4526.288 kb on + strand, within Pf1N1B4_4075 at 4526.288 kb on + strand, within Pf1N1B4_4075 at 4526.288 kb on + strand at 4526.289 kb on - strand, within Pf1N1B4_4075 at 4526.291 kb on + strand, within Pf1N1B4_4075 at 4526.352 kb on - strand at 4526.352 kb on - strand at 4526.352 kb on - strand at 4526.352 kb on - strand at 4526.352 kb on - strand at 4526.352 kb on - strand at 4526.411 kb on + strand at 4526.411 kb on + strand at 4526.412 kb on - strand at 4526.412 kb on - strand at 4526.412 kb on - strand at 4526.412 kb on - strand at 4526.464 kb on - strand at 4526.464 kb on - strand at 4526.464 kb on - strand at 4526.469 kb on - strand at 4526.469 kb on - strand at 4526.536 kb on + strand, within Pf1N1B4_4076 at 4526.562 kb on - strand, within Pf1N1B4_4076 at 4526.718 kb on - strand, within Pf1N1B4_4076 at 4526.733 kb on - strand, within Pf1N1B4_4076 at 4526.818 kb on - strand, within Pf1N1B4_4076 at 4526.820 kb on - strand at 4526.820 kb on - strand, within Pf1N1B4_4076 at 4526.820 kb on - strand, within Pf1N1B4_4076 at 4526.820 kb on - strand, within Pf1N1B4_4076 at 4526.895 kb on + strand at 4526.896 kb on - strand at 4526.896 kb on - strand at 4526.900 kb on + strand at 4526.901 kb on - strand at 4526.901 kb on - strand at 4526.998 kb on - strand at 4527.145 kb on + strand, within Pf1N1B4_4077 at 4527.146 kb on - strand, within Pf1N1B4_4077 at 4527.202 kb on + strand, within Pf1N1B4_4077 at 4527.203 kb on - strand at 4527.241 kb on - strand, within Pf1N1B4_4077 at 4527.448 kb on + strand at 4527.460 kb on + strand at 4527.461 kb on - strand at 4527.461 kb on - strand at 4527.565 kb on + strand at 4527.566 kb on - strand at 4527.641 kb on + strand, within Pf1N1B4_4078 at 4527.641 kb on + strand, within Pf1N1B4_4078 at 4527.669 kb on - strand, within Pf1N1B4_4078 at 4527.713 kb on + strand, within Pf1N1B4_4078
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1 remove 4,525,594 + -0.0 4,525,717 - Pf1N1B4_4074 0.51 -3.6 4,525,818 + -1.6 4,525,818 + -1.6 4,525,821 + -0.0 4,525,822 - -2.4 4,525,931 + -0.8 4,525,931 + -0.0 4,525,932 - -2.8 4,525,971 + -0.0 4,525,971 + -1.5 4,525,972 - -2.1 4,526,090 + Pf1N1B4_4075 0.25 -0.0 4,526,189 + Pf1N1B4_4075 0.53 -1.7 4,526,190 - Pf1N1B4_4075 0.53 -0.0 4,526,190 - Pf1N1B4_4075 0.53 -1.2 4,526,190 - Pf1N1B4_4075 0.53 -5.4 4,526,225 + Pf1N1B4_4075 0.63 -0.6 4,526,225 + Pf1N1B4_4075 0.63 -0.0 4,526,225 + Pf1N1B4_4075 0.63 -1.6 4,526,225 + Pf1N1B4_4075 0.63 -0.0 4,526,225 + Pf1N1B4_4075 0.63 -1.5 4,526,225 + Pf1N1B4_4075 0.63 -0.0 4,526,225 + -0.0 4,526,226 - Pf1N1B4_4075 0.64 -1.6 4,526,251 - Pf1N1B4_4075 0.71 -0.0 4,526,288 + Pf1N1B4_4075 0.81 -3.2 4,526,288 + Pf1N1B4_4075 0.81 -0.0 4,526,288 + Pf1N1B4_4075 0.81 -1.4 4,526,288 + -0.0 4,526,289 - Pf1N1B4_4075 0.81 -0.6 4,526,291 + Pf1N1B4_4075 0.82 -1.6 4,526,352 - -2.5 4,526,352 - -0.0 4,526,352 - -0.0 4,526,352 - -0.0 4,526,352 - -1.7 4,526,352 - -0.1 4,526,411 + -0.0 4,526,411 + -0.0 4,526,412 - -0.0 4,526,412 - -1.0 4,526,412 - -2.2 4,526,412 - -0.0 4,526,464 - -2.1 4,526,464 - -1.4 4,526,464 - -0.0 4,526,469 - -0.0 4,526,469 - -2.2 4,526,536 + Pf1N1B4_4076 0.16 -1.9 4,526,562 - Pf1N1B4_4076 0.22 -0.0 4,526,718 - Pf1N1B4_4076 0.59 -0.0 4,526,733 - Pf1N1B4_4076 0.62 -0.0 4,526,818 - Pf1N1B4_4076 0.82 -0.0 4,526,820 - -0.0 4,526,820 - Pf1N1B4_4076 0.82 -0.0 4,526,820 - Pf1N1B4_4076 0.82 -3.3 4,526,820 - Pf1N1B4_4076 0.82 -2.7 4,526,895 + -0.0 4,526,896 - -0.0 4,526,896 - -0.0 4,526,900 + -2.1 4,526,901 - -0.0 4,526,901 - -1.8 4,526,998 - -0.0 4,527,145 + Pf1N1B4_4077 0.23 -0.8 4,527,146 - Pf1N1B4_4077 0.24 -0.0 4,527,202 + Pf1N1B4_4077 0.36 -0.0 4,527,203 - -1.4 4,527,241 - Pf1N1B4_4077 0.45 -0.0 4,527,448 + -1.3 4,527,460 + -0.0 4,527,461 - -1.9 4,527,461 - -0.0 4,527,565 + -0.0 4,527,566 - -1.5 4,527,641 + Pf1N1B4_4078 0.10 -2.7 4,527,641 + Pf1N1B4_4078 0.10 -1.0 4,527,669 - Pf1N1B4_4078 0.15 -0.0 4,527,713 + Pf1N1B4_4078 0.22 -1.2
Or see this region's nucleotide sequence