Experiment: Nitrate reduction, replicate A t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3888 and Pf1N1B4_3889 are separated by 60 nucleotides Pf1N1B4_3889 and Pf1N1B4_3890 overlap by 21 nucleotides
Pf1N1B4_3888 - Aconitate hydratase (EC 4.2.1.3), at 4,317,018 to 4,319,759
_3888
Pf1N1B4_3889 - hypothetical protein, at 4,319,820 to 4,320,002
_3889
Pf1N1B4_3890 - LSU rRNA 2'-O-methyl-C2498 methyltransferase RlmM, at 4,319,982 to 4,321,055
_3890
Position (kb) 4319
4320
4321 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4319.155 kb on + strand, within Pf1N1B4_3888 at 4319.155 kb on + strand, within Pf1N1B4_3888 at 4319.155 kb on + strand, within Pf1N1B4_3888 at 4319.155 kb on + strand, within Pf1N1B4_3888 at 4319.155 kb on + strand, within Pf1N1B4_3888 at 4319.155 kb on + strand, within Pf1N1B4_3888 at 4319.156 kb on - strand, within Pf1N1B4_3888 at 4319.156 kb on - strand, within Pf1N1B4_3888 at 4319.156 kb on - strand, within Pf1N1B4_3888 at 4319.156 kb on - strand, within Pf1N1B4_3888 at 4319.156 kb on - strand, within Pf1N1B4_3888 at 4319.156 kb on - strand, within Pf1N1B4_3888 at 4319.156 kb on - strand, within Pf1N1B4_3888 at 4319.201 kb on - strand, within Pf1N1B4_3888 at 4319.201 kb on - strand, within Pf1N1B4_3888 at 4319.259 kb on - strand, within Pf1N1B4_3888 at 4319.353 kb on + strand at 4319.353 kb on + strand, within Pf1N1B4_3888 at 4319.354 kb on - strand, within Pf1N1B4_3888 at 4319.354 kb on - strand, within Pf1N1B4_3888 at 4319.502 kb on + strand at 4319.701 kb on + strand at 4319.701 kb on + strand at 4319.701 kb on + strand at 4319.702 kb on - strand at 4319.721 kb on + strand at 4319.722 kb on - strand at 4319.774 kb on + strand at 4319.774 kb on + strand at 4319.774 kb on + strand at 4319.775 kb on - strand at 4319.816 kb on - strand at 4319.816 kb on - strand at 4319.831 kb on + strand at 4319.833 kb on + strand at 4319.833 kb on + strand at 4319.833 kb on + strand at 4319.834 kb on - strand at 4319.847 kb on + strand, within Pf1N1B4_3889 at 4319.859 kb on - strand, within Pf1N1B4_3889 at 4319.977 kb on + strand, within Pf1N1B4_3889 at 4319.977 kb on + strand, within Pf1N1B4_3889 at 4319.996 kb on + strand at 4320.900 kb on + strand, within Pf1N1B4_3890 at 4320.900 kb on + strand, within Pf1N1B4_3890 at 4320.900 kb on + strand, within Pf1N1B4_3890 at 4320.929 kb on + strand, within Pf1N1B4_3890 at 4320.987 kb on + strand at 4320.987 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1 remove 4,319,155 + Pf1N1B4_3888 0.78 -1.6 4,319,155 + Pf1N1B4_3888 0.78 -0.0 4,319,155 + Pf1N1B4_3888 0.78 -0.0 4,319,155 + Pf1N1B4_3888 0.78 -0.0 4,319,155 + Pf1N1B4_3888 0.78 -1.7 4,319,155 + Pf1N1B4_3888 0.78 -0.0 4,319,156 - Pf1N1B4_3888 0.78 -0.0 4,319,156 - Pf1N1B4_3888 0.78 -0.0 4,319,156 - Pf1N1B4_3888 0.78 -0.0 4,319,156 - Pf1N1B4_3888 0.78 -0.0 4,319,156 - Pf1N1B4_3888 0.78 -1.5 4,319,156 - Pf1N1B4_3888 0.78 -0.7 4,319,156 - Pf1N1B4_3888 0.78 -0.0 4,319,201 - Pf1N1B4_3888 0.80 -1.7 4,319,201 - Pf1N1B4_3888 0.80 -3.0 4,319,259 - Pf1N1B4_3888 0.82 -0.0 4,319,353 + -0.0 4,319,353 + Pf1N1B4_3888 0.85 -0.0 4,319,354 - Pf1N1B4_3888 0.85 -0.0 4,319,354 - Pf1N1B4_3888 0.85 -0.0 4,319,502 + -1.0 4,319,701 + -0.0 4,319,701 + -0.0 4,319,701 + -1.3 4,319,702 - -0.0 4,319,721 + -0.0 4,319,722 - -1.3 4,319,774 + -0.0 4,319,774 + -0.0 4,319,774 + -2.0 4,319,775 - -0.0 4,319,816 - -0.0 4,319,816 - -0.0 4,319,831 + -0.9 4,319,833 + -2.6 4,319,833 + -0.9 4,319,833 + -2.3 4,319,834 - -0.0 4,319,847 + Pf1N1B4_3889 0.15 -0.0 4,319,859 - Pf1N1B4_3889 0.21 -0.0 4,319,977 + Pf1N1B4_3889 0.86 -0.0 4,319,977 + Pf1N1B4_3889 0.86 -2.6 4,319,996 + -0.0 4,320,900 + Pf1N1B4_3890 0.85 -0.0 4,320,900 + Pf1N1B4_3890 0.85 -0.0 4,320,900 + Pf1N1B4_3890 0.85 -0.0 4,320,929 + Pf1N1B4_3890 0.88 -0.0 4,320,987 + -0.0 4,320,987 + -0.0
Or see this region's nucleotide sequence