Experiment: Nitrate reduction, replicate A t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3764 and Pf1N1B4_3765 are separated by 88 nucleotides Pf1N1B4_3765 and Pf1N1B4_3766 overlap by 4 nucleotides Pf1N1B4_3766 and Pf1N1B4_3767 are separated by 459 nucleotides
Pf1N1B4_3764 - Allantoicase (EC 3.5.3.4), at 4,175,252 to 4,176,247
_3764
Pf1N1B4_3765 - 2-oxo-4-hydroxy-4-carboxy--5-ureidoimidazoline (OHCU) decarboxylase, at 4,176,336 to 4,176,851
_3765
Pf1N1B4_3766 - Uricase (urate oxidase) (EC 1.7.3.3), at 4,176,848 to 4,177,774
_3766
Pf1N1B4_3767 - 5-Hydroxyisourate Hydrolase (HIUase) (EC 3.5.2.17), at 4,178,234 to 4,178,563
_3767
Position (kb) 4176
4177
4178 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4176.237 kb on + strand at 4176.237 kb on + strand at 4176.264 kb on + strand at 4176.265 kb on - strand at 4176.268 kb on + strand at 4176.268 kb on + strand at 4176.268 kb on + strand at 4176.268 kb on + strand at 4176.269 kb on - strand at 4176.339 kb on + strand at 4176.339 kb on + strand at 4176.340 kb on - strand at 4176.466 kb on - strand, within Pf1N1B4_3765 at 4176.502 kb on + strand, within Pf1N1B4_3765 at 4176.503 kb on - strand, within Pf1N1B4_3765 at 4176.503 kb on - strand, within Pf1N1B4_3765 at 4176.503 kb on - strand at 4176.778 kb on + strand, within Pf1N1B4_3765 at 4176.778 kb on + strand, within Pf1N1B4_3765 at 4176.778 kb on + strand, within Pf1N1B4_3765 at 4176.778 kb on + strand, within Pf1N1B4_3765 at 4176.778 kb on + strand, within Pf1N1B4_3765 at 4176.778 kb on + strand, within Pf1N1B4_3765 at 4176.778 kb on + strand, within Pf1N1B4_3765 at 4176.778 kb on + strand, within Pf1N1B4_3765 at 4176.778 kb on + strand, within Pf1N1B4_3765 at 4176.779 kb on - strand, within Pf1N1B4_3765 at 4176.779 kb on - strand, within Pf1N1B4_3765 at 4176.779 kb on - strand, within Pf1N1B4_3765 at 4176.779 kb on - strand, within Pf1N1B4_3765 at 4176.779 kb on - strand, within Pf1N1B4_3765 at 4176.937 kb on + strand at 4177.044 kb on + strand, within Pf1N1B4_3766 at 4177.044 kb on + strand, within Pf1N1B4_3766 at 4177.045 kb on - strand, within Pf1N1B4_3766 at 4177.144 kb on - strand at 4177.209 kb on + strand, within Pf1N1B4_3766 at 4177.209 kb on + strand, within Pf1N1B4_3766 at 4177.209 kb on + strand, within Pf1N1B4_3766 at 4177.210 kb on - strand, within Pf1N1B4_3766 at 4177.210 kb on - strand, within Pf1N1B4_3766 at 4177.284 kb on + strand, within Pf1N1B4_3766 at 4177.285 kb on - strand, within Pf1N1B4_3766 at 4177.371 kb on + strand, within Pf1N1B4_3766 at 4177.371 kb on + strand, within Pf1N1B4_3766 at 4177.371 kb on + strand, within Pf1N1B4_3766 at 4177.371 kb on + strand, within Pf1N1B4_3766 at 4177.372 kb on - strand, within Pf1N1B4_3766 at 4177.372 kb on - strand, within Pf1N1B4_3766 at 4177.377 kb on + strand, within Pf1N1B4_3766 at 4177.545 kb on + strand, within Pf1N1B4_3766 at 4177.545 kb on + strand, within Pf1N1B4_3766 at 4177.671 kb on + strand, within Pf1N1B4_3766 at 4177.671 kb on + strand, within Pf1N1B4_3766 at 4177.672 kb on - strand, within Pf1N1B4_3766 at 4177.672 kb on - strand, within Pf1N1B4_3766 at 4177.672 kb on - strand, within Pf1N1B4_3766 at 4177.887 kb on + strand at 4177.889 kb on + strand at 4177.890 kb on - strand at 4177.900 kb on + strand at 4177.983 kb on + strand at 4177.983 kb on + strand at 4177.983 kb on + strand at 4177.983 kb on + strand at 4177.983 kb on + strand at 4177.984 kb on - strand at 4178.001 kb on + strand at 4178.001 kb on + strand at 4178.001 kb on + strand at 4178.001 kb on + strand at 4178.002 kb on - strand at 4178.002 kb on - strand at 4178.002 kb on - strand at 4178.068 kb on - strand at 4178.225 kb on - strand at 4178.282 kb on + strand, within Pf1N1B4_3767 at 4178.282 kb on + strand, within Pf1N1B4_3767 at 4178.390 kb on + strand, within Pf1N1B4_3767 at 4178.390 kb on + strand, within Pf1N1B4_3767 at 4178.390 kb on + strand, within Pf1N1B4_3767 at 4178.417 kb on + strand, within Pf1N1B4_3767 at 4178.417 kb on + strand, within Pf1N1B4_3767 at 4178.417 kb on + strand, within Pf1N1B4_3767 at 4178.417 kb on + strand, within Pf1N1B4_3767 at 4178.418 kb on - strand, within Pf1N1B4_3767 at 4178.507 kb on + strand, within Pf1N1B4_3767 at 4178.534 kb on + strand at 4178.534 kb on + strand at 4178.535 kb on - strand at 4178.540 kb on + strand at 4178.540 kb on + strand at 4178.540 kb on + strand at 4178.540 kb on + strand at 4178.540 kb on + strand at 4178.540 kb on + strand at 4178.540 kb on + strand at 4178.540 kb on + strand at 4178.541 kb on - strand at 4178.541 kb on - strand at 4178.549 kb on + strand at 4178.603 kb on + strand at 4178.603 kb on + strand at 4178.603 kb on + strand at 4178.603 kb on + strand at 4178.603 kb on + strand at 4178.603 kb on + strand at 4178.603 kb on + strand at 4178.604 kb on - strand at 4178.604 kb on - strand at 4178.604 kb on - strand at 4178.605 kb on + strand at 4178.605 kb on + strand at 4178.605 kb on + strand at 4178.605 kb on + strand at 4178.605 kb on + strand at 4178.605 kb on + strand at 4178.605 kb on + strand at 4178.605 kb on + strand at 4178.605 kb on + strand at 4178.605 kb on + strand at 4178.605 kb on + strand at 4178.605 kb on + strand at 4178.605 kb on + strand at 4178.605 kb on + strand at 4178.606 kb on - strand at 4178.606 kb on - strand at 4178.606 kb on - strand at 4178.606 kb on - strand at 4178.606 kb on - strand at 4178.606 kb on - strand at 4178.606 kb on - strand at 4178.606 kb on - strand at 4178.606 kb on - strand at 4178.606 kb on - strand at 4178.606 kb on - strand at 4178.725 kb on - strand at 4178.725 kb on - strand at 4178.725 kb on - strand at 4178.729 kb on - strand at 4178.729 kb on - strand at 4178.729 kb on - strand at 4178.729 kb on - strand at 4178.729 kb on - strand at 4178.729 kb on - strand at 4178.729 kb on - strand at 4178.729 kb on - strand at 4178.729 kb on - strand at 4178.729 kb on - strand at 4178.734 kb on - strand at 4178.734 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1 remove 4,176,237 + -1.6 4,176,237 + -1.0 4,176,264 + -0.0 4,176,265 - -0.8 4,176,268 + -1.6 4,176,268 + -0.0 4,176,268 + -0.0 4,176,268 + -0.0 4,176,269 - -2.2 4,176,339 + -0.0 4,176,339 + -0.0 4,176,340 - -0.0 4,176,466 - Pf1N1B4_3765 0.25 -1.6 4,176,502 + Pf1N1B4_3765 0.32 -0.0 4,176,503 - Pf1N1B4_3765 0.32 -0.0 4,176,503 - Pf1N1B4_3765 0.32 -0.0 4,176,503 - -0.0 4,176,778 + Pf1N1B4_3765 0.86 -0.0 4,176,778 + Pf1N1B4_3765 0.86 -1.5 4,176,778 + Pf1N1B4_3765 0.86 -3.0 4,176,778 + Pf1N1B4_3765 0.86 -0.0 4,176,778 + Pf1N1B4_3765 0.86 -3.0 4,176,778 + Pf1N1B4_3765 0.86 -0.0 4,176,778 + Pf1N1B4_3765 0.86 -1.5 4,176,778 + Pf1N1B4_3765 0.86 -0.0 4,176,778 + Pf1N1B4_3765 0.86 -1.9 4,176,779 - Pf1N1B4_3765 0.86 -0.0 4,176,779 - Pf1N1B4_3765 0.86 -0.9 4,176,779 - Pf1N1B4_3765 0.86 -1.8 4,176,779 - Pf1N1B4_3765 0.86 -1.3 4,176,779 - Pf1N1B4_3765 0.86 -3.1 4,176,937 + -0.0 4,177,044 + Pf1N1B4_3766 0.21 -0.0 4,177,044 + Pf1N1B4_3766 0.21 -0.0 4,177,045 - Pf1N1B4_3766 0.21 -0.0 4,177,144 - -0.0 4,177,209 + Pf1N1B4_3766 0.39 -0.0 4,177,209 + Pf1N1B4_3766 0.39 -2.6 4,177,209 + Pf1N1B4_3766 0.39 -0.0 4,177,210 - Pf1N1B4_3766 0.39 -0.0 4,177,210 - Pf1N1B4_3766 0.39 -0.0 4,177,284 + Pf1N1B4_3766 0.47 -0.0 4,177,285 - Pf1N1B4_3766 0.47 -2.8 4,177,371 + Pf1N1B4_3766 0.56 -3.5 4,177,371 + Pf1N1B4_3766 0.56 -0.0 4,177,371 + Pf1N1B4_3766 0.56 -2.2 4,177,371 + Pf1N1B4_3766 0.56 -3.4 4,177,372 - Pf1N1B4_3766 0.57 -0.0 4,177,372 - Pf1N1B4_3766 0.57 -2.8 4,177,377 + Pf1N1B4_3766 0.57 -3.3 4,177,545 + Pf1N1B4_3766 0.75 -0.0 4,177,545 + Pf1N1B4_3766 0.75 -0.0 4,177,671 + Pf1N1B4_3766 0.89 -0.0 4,177,671 + Pf1N1B4_3766 0.89 -0.0 4,177,672 - Pf1N1B4_3766 0.89 -0.0 4,177,672 - Pf1N1B4_3766 0.89 -0.6 4,177,672 - Pf1N1B4_3766 0.89 -0.0 4,177,887 + -0.0 4,177,889 + -0.0 4,177,890 - -0.0 4,177,900 + -0.0 4,177,983 + -0.0 4,177,983 + -1.0 4,177,983 + -1.0 4,177,983 + +1.1 4,177,983 + -1.7 4,177,984 - -0.0 4,178,001 + -1.7 4,178,001 + -0.0 4,178,001 + -2.1 4,178,001 + -0.0 4,178,002 - -0.0 4,178,002 - -0.4 4,178,002 - -0.0 4,178,068 - -0.4 4,178,225 - -0.0 4,178,282 + Pf1N1B4_3767 0.15 -0.0 4,178,282 + Pf1N1B4_3767 0.15 -1.6 4,178,390 + Pf1N1B4_3767 0.47 -1.2 4,178,390 + Pf1N1B4_3767 0.47 -0.0 4,178,390 + Pf1N1B4_3767 0.47 -2.3 4,178,417 + Pf1N1B4_3767 0.55 -0.0 4,178,417 + Pf1N1B4_3767 0.55 -1.2 4,178,417 + Pf1N1B4_3767 0.55 -0.0 4,178,417 + Pf1N1B4_3767 0.55 -1.9 4,178,418 - Pf1N1B4_3767 0.56 -0.0 4,178,507 + Pf1N1B4_3767 0.83 -1.3 4,178,534 + -1.6 4,178,534 + -0.0 4,178,535 - -0.0 4,178,540 + -0.9 4,178,540 + -1.0 4,178,540 + -0.0 4,178,540 + -1.2 4,178,540 + -0.2 4,178,540 + -2.2 4,178,540 + -0.0 4,178,540 + -0.0 4,178,541 - -1.7 4,178,541 - -1.8 4,178,549 + -2.8 4,178,603 + -0.0 4,178,603 + -1.6 4,178,603 + -0.0 4,178,603 + -0.0 4,178,603 + -1.9 4,178,603 + -0.0 4,178,603 + -0.0 4,178,604 - -1.9 4,178,604 - -0.0 4,178,604 - -0.0 4,178,605 + -3.0 4,178,605 + -1.7 4,178,605 + -2.4 4,178,605 + -0.0 4,178,605 + -0.0 4,178,605 + -0.0 4,178,605 + -0.0 4,178,605 + -1.4 4,178,605 + -0.0 4,178,605 + -0.0 4,178,605 + -1.5 4,178,605 + -0.0 4,178,605 + -1.4 4,178,605 + -2.2 4,178,606 - -2.6 4,178,606 - -0.0 4,178,606 - -1.9 4,178,606 - -0.0 4,178,606 - -0.0 4,178,606 - -1.2 4,178,606 - -0.0 4,178,606 - -1.8 4,178,606 - -0.0 4,178,606 - -2.2 4,178,606 - -1.2 4,178,725 - -0.0 4,178,725 - -0.0 4,178,725 - -0.0 4,178,729 - -1.9 4,178,729 - -1.2 4,178,729 - -0.0 4,178,729 - -2.1 4,178,729 - -1.1 4,178,729 - -0.0 4,178,729 - -0.0 4,178,729 - -1.6 4,178,729 - -0.0 4,178,729 - -0.0 4,178,734 - -0.0 4,178,734 - -0.8
Or see this region's nucleotide sequence