Experiment: Nitrate reduction, replicate A t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_336 and Pf1N1B4_337 overlap by 11 nucleotides Pf1N1B4_337 and Pf1N1B4_338 are separated by 117 nucleotides Pf1N1B4_338 and Pf1N1B4_339 are separated by 359 nucleotides
Pf1N1B4_336 - Teichoic acid export ATP-binding protein TagH (EC 3.6.3.40), at 399,494 to 400,861
_336
Pf1N1B4_337 - O-antigen export system permease protein RfbD, at 400,851 to 401,669
_337
Pf1N1B4_338 - Alpha-L-Rha alpha-1,3-L-rhamnosyltransferase (EC 2.4.1.-), at 401,787 to 402,794
_338
Pf1N1B4_339 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 403,154 to 404,116
_339
Position (kb) 401
402
403 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 400.806 kb on + strand at 400.807 kb on - strand at 400.807 kb on - strand at 400.810 kb on + strand at 400.810 kb on + strand at 400.811 kb on - strand at 400.849 kb on + strand at 400.850 kb on - strand at 400.850 kb on - strand at 400.850 kb on - strand at 400.850 kb on - strand at 400.850 kb on - strand at 400.852 kb on + strand at 400.874 kb on + strand at 400.928 kb on + strand at 400.929 kb on - strand at 400.930 kb on + strand at 400.931 kb on - strand at 400.931 kb on - strand at 400.931 kb on - strand at 400.931 kb on - strand at 400.931 kb on - strand at 400.931 kb on - strand at 400.931 kb on - strand at 400.931 kb on - strand at 400.931 kb on - strand at 400.933 kb on + strand, within Pf1N1B4_337 at 400.933 kb on + strand, within Pf1N1B4_337 at 400.934 kb on - strand, within Pf1N1B4_337 at 400.934 kb on - strand at 400.934 kb on - strand, within Pf1N1B4_337 at 400.934 kb on - strand, within Pf1N1B4_337 at 400.960 kb on - strand, within Pf1N1B4_337 at 400.960 kb on - strand, within Pf1N1B4_337 at 401.216 kb on + strand, within Pf1N1B4_337 at 401.217 kb on - strand, within Pf1N1B4_337 at 401.217 kb on - strand, within Pf1N1B4_337 at 401.264 kb on + strand, within Pf1N1B4_337 at 401.316 kb on + strand, within Pf1N1B4_337 at 401.344 kb on - strand, within Pf1N1B4_337 at 401.386 kb on - strand, within Pf1N1B4_337 at 401.405 kb on + strand, within Pf1N1B4_337 at 401.406 kb on - strand, within Pf1N1B4_337 at 401.406 kb on - strand, within Pf1N1B4_337 at 401.432 kb on + strand, within Pf1N1B4_337 at 401.432 kb on + strand, within Pf1N1B4_337 at 401.655 kb on - strand at 401.675 kb on - strand at 401.735 kb on + strand at 402.084 kb on - strand, within Pf1N1B4_338 at 402.086 kb on - strand, within Pf1N1B4_338 at 402.086 kb on - strand, within Pf1N1B4_338 at 402.145 kb on + strand, within Pf1N1B4_338 at 402.198 kb on + strand, within Pf1N1B4_338 at 402.443 kb on - strand, within Pf1N1B4_338 at 402.474 kb on - strand at 402.554 kb on - strand, within Pf1N1B4_338 at 402.881 kb on - strand at 402.930 kb on - strand at 403.004 kb on - strand at 403.016 kb on + strand at 403.017 kb on - strand at 403.017 kb on - strand at 403.017 kb on - strand at 403.017 kb on - strand at 403.148 kb on + strand at 403.148 kb on + strand at 403.149 kb on - strand at 403.159 kb on + strand at 403.493 kb on + strand, within Pf1N1B4_339 at 403.765 kb on + strand, within Pf1N1B4_339
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1 remove 400,806 + -2.5 400,807 - -0.0 400,807 - -0.0 400,810 + -4.0 400,810 + -4.6 400,811 - -0.0 400,849 + -0.0 400,850 - -0.0 400,850 - -1.1 400,850 - -0.8 400,850 - -2.1 400,850 - -0.0 400,852 + -1.3 400,874 + -3.9 400,928 + -0.0 400,929 - -1.0 400,930 + -0.0 400,931 - -3.2 400,931 - -0.0 400,931 - -1.7 400,931 - -1.3 400,931 - -3.7 400,931 - -0.0 400,931 - -0.0 400,931 - -0.0 400,931 - -0.0 400,933 + Pf1N1B4_337 0.10 -0.0 400,933 + Pf1N1B4_337 0.10 -0.0 400,934 - Pf1N1B4_337 0.10 -1.8 400,934 - -2.8 400,934 - Pf1N1B4_337 0.10 -2.3 400,934 - Pf1N1B4_337 0.10 -2.6 400,960 - Pf1N1B4_337 0.13 -3.1 400,960 - Pf1N1B4_337 0.13 -0.0 401,216 + Pf1N1B4_337 0.45 -0.0 401,217 - Pf1N1B4_337 0.45 -4.6 401,217 - Pf1N1B4_337 0.45 -0.0 401,264 + Pf1N1B4_337 0.50 -2.7 401,316 + Pf1N1B4_337 0.57 -3.2 401,344 - Pf1N1B4_337 0.60 -0.0 401,386 - Pf1N1B4_337 0.65 -0.0 401,405 + Pf1N1B4_337 0.68 -0.0 401,406 - Pf1N1B4_337 0.68 -0.0 401,406 - Pf1N1B4_337 0.68 -2.3 401,432 + Pf1N1B4_337 0.71 -0.0 401,432 + Pf1N1B4_337 0.71 -0.9 401,655 - -0.0 401,675 - -0.0 401,735 + -0.0 402,084 - Pf1N1B4_338 0.29 -0.0 402,086 - Pf1N1B4_338 0.30 -0.0 402,086 - Pf1N1B4_338 0.30 -2.6 402,145 + Pf1N1B4_338 0.36 -2.5 402,198 + Pf1N1B4_338 0.41 -0.0 402,443 - Pf1N1B4_338 0.65 -0.0 402,474 - +0.5 402,554 - Pf1N1B4_338 0.76 -0.0 402,881 - -1.6 402,930 - -3.8 403,004 - -0.0 403,016 + -0.0 403,017 - -0.3 403,017 - -0.9 403,017 - -1.6 403,017 - -3.1 403,148 + -0.0 403,148 + -5.3 403,149 - -0.0 403,159 + -0.9 403,493 + Pf1N1B4_339 0.35 -2.7 403,765 + Pf1N1B4_339 0.63 -0.6
Or see this region's nucleotide sequence