Experiment: Nitrate reduction, replicate A t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2984 and Pf1N1B4_2985 are separated by 166 nucleotides Pf1N1B4_2985 and Pf1N1B4_2986 overlap by 4 nucleotides Pf1N1B4_2986 and Pf1N1B4_2987 are separated by 150 nucleotides Pf1N1B4_2987 and Pf1N1B4_2988 overlap by 4 nucleotides
Pf1N1B4_2984 - VacJ-like lipoprotein precursor, at 3,351,561 to 3,352,352
_2984
Pf1N1B4_2985 - DNA-binding response regulator GltR, controls specific porins for the entry of glucose, at 3,352,519 to 3,353,229
_2985
Pf1N1B4_2986 - sensor histidine kinase, at 3,353,226 to 3,354,611
_2986
Pf1N1B4_2987 - NAD-dependent formate dehydrogenase delta subunit, at 3,354,762 to 3,354,983
_2987
Pf1N1B4_2988 - NAD-dependent formate dehydrogenase alpha subunit, at 3,354,980 to 3,357,862
_2988
Position (kb) 3353
3354
3355 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 3352.226 kb on - strand, within Pf1N1B4_2984 at 3352.421 kb on - strand at 3352.455 kb on + strand at 3352.455 kb on + strand at 3352.455 kb on + strand at 3352.455 kb on + strand at 3352.455 kb on + strand at 3352.455 kb on + strand at 3352.456 kb on - strand at 3352.456 kb on - strand at 3352.456 kb on - strand at 3352.456 kb on - strand at 3352.456 kb on - strand at 3352.456 kb on - strand at 3352.456 kb on - strand at 3352.457 kb on + strand at 3352.458 kb on - strand at 3352.458 kb on - strand at 3352.458 kb on - strand at 3352.482 kb on + strand at 3352.482 kb on + strand at 3352.482 kb on + strand at 3352.483 kb on - strand at 3352.483 kb on - strand at 3352.483 kb on - strand at 3352.483 kb on - strand at 3352.506 kb on + strand at 3352.507 kb on - strand at 3352.507 kb on - strand at 3352.532 kb on + strand at 3352.533 kb on - strand at 3352.565 kb on + strand at 3352.566 kb on - strand at 3352.810 kb on + strand, within Pf1N1B4_2985 at 3352.811 kb on - strand, within Pf1N1B4_2985 at 3352.811 kb on - strand, within Pf1N1B4_2985 at 3353.206 kb on - strand at 3353.206 kb on - strand at 3353.270 kb on + strand at 3353.270 kb on + strand at 3353.271 kb on - strand at 3353.359 kb on + strand at 3353.360 kb on - strand at 3353.360 kb on - strand at 3353.360 kb on - strand at 3353.610 kb on + strand, within Pf1N1B4_2986 at 3353.610 kb on + strand, within Pf1N1B4_2986 at 3353.611 kb on - strand, within Pf1N1B4_2986 at 3353.611 kb on - strand, within Pf1N1B4_2986 at 3353.739 kb on + strand, within Pf1N1B4_2986 at 3353.740 kb on - strand, within Pf1N1B4_2986 at 3353.941 kb on + strand, within Pf1N1B4_2986 at 3353.941 kb on + strand, within Pf1N1B4_2986 at 3353.941 kb on + strand, within Pf1N1B4_2986 at 3353.942 kb on - strand, within Pf1N1B4_2986 at 3353.942 kb on - strand, within Pf1N1B4_2986 at 3353.942 kb on - strand, within Pf1N1B4_2986 at 3354.192 kb on + strand, within Pf1N1B4_2986 at 3354.215 kb on + strand, within Pf1N1B4_2986 at 3354.215 kb on + strand, within Pf1N1B4_2986 at 3354.215 kb on + strand, within Pf1N1B4_2986 at 3354.215 kb on + strand, within Pf1N1B4_2986 at 3354.216 kb on - strand, within Pf1N1B4_2986 at 3354.216 kb on - strand, within Pf1N1B4_2986 at 3354.243 kb on + strand, within Pf1N1B4_2986 at 3354.243 kb on + strand, within Pf1N1B4_2986 at 3354.243 kb on + strand, within Pf1N1B4_2986 at 3354.244 kb on - strand, within Pf1N1B4_2986 at 3354.244 kb on - strand, within Pf1N1B4_2986 at 3354.266 kb on + strand, within Pf1N1B4_2986 at 3354.267 kb on - strand, within Pf1N1B4_2986 at 3354.278 kb on + strand, within Pf1N1B4_2986 at 3354.306 kb on + strand, within Pf1N1B4_2986 at 3354.306 kb on + strand, within Pf1N1B4_2986 at 3354.307 kb on - strand, within Pf1N1B4_2986 at 3354.364 kb on + strand, within Pf1N1B4_2986 at 3354.385 kb on + strand, within Pf1N1B4_2986 at 3354.386 kb on - strand, within Pf1N1B4_2986 at 3354.562 kb on + strand at 3354.563 kb on - strand at 3354.604 kb on + strand at 3354.604 kb on + strand at 3354.605 kb on - strand at 3354.605 kb on - strand at 3354.697 kb on + strand at 3354.697 kb on + strand at 3354.698 kb on - strand at 3354.698 kb on - strand at 3354.747 kb on + strand at 3354.747 kb on + strand at 3354.747 kb on + strand at 3354.747 kb on + strand at 3354.748 kb on - strand at 3354.748 kb on - strand at 3354.763 kb on + strand at 3354.763 kb on + strand at 3354.763 kb on + strand at 3354.763 kb on + strand at 3354.764 kb on - strand at 3354.764 kb on - strand at 3354.874 kb on + strand, within Pf1N1B4_2987 at 3354.874 kb on + strand, within Pf1N1B4_2987 at 3354.874 kb on + strand, within Pf1N1B4_2987 at 3354.874 kb on + strand at 3354.875 kb on - strand, within Pf1N1B4_2987 at 3354.875 kb on - strand, within Pf1N1B4_2987 at 3354.875 kb on - strand, within Pf1N1B4_2987 at 3354.875 kb on - strand, within Pf1N1B4_2987 at 3354.937 kb on + strand, within Pf1N1B4_2987 at 3354.937 kb on + strand, within Pf1N1B4_2987 at 3354.937 kb on + strand, within Pf1N1B4_2987 at 3354.938 kb on - strand, within Pf1N1B4_2987 at 3354.938 kb on - strand, within Pf1N1B4_2987 at 3354.990 kb on - strand at 3355.089 kb on + strand at 3355.089 kb on + strand at 3355.090 kb on - strand at 3355.122 kb on + strand at 3355.123 kb on - strand at 3355.123 kb on - strand at 3355.123 kb on - strand at 3355.203 kb on + strand at 3355.203 kb on + strand at 3355.203 kb on + strand at 3355.203 kb on + strand at 3355.203 kb on + strand at 3355.203 kb on + strand at 3355.203 kb on + strand at 3355.204 kb on - strand at 3355.204 kb on - strand at 3355.204 kb on - strand at 3355.204 kb on - strand at 3355.204 kb on - strand at 3355.204 kb on - strand at 3355.247 kb on + strand at 3355.392 kb on + strand, within Pf1N1B4_2988 at 3355.392 kb on + strand, within Pf1N1B4_2988 at 3355.392 kb on + strand, within Pf1N1B4_2988 at 3355.392 kb on + strand, within Pf1N1B4_2988 at 3355.393 kb on - strand, within Pf1N1B4_2988 at 3355.393 kb on - strand, within Pf1N1B4_2988 at 3355.587 kb on + strand, within Pf1N1B4_2988 at 3355.588 kb on - strand, within Pf1N1B4_2988
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1 remove 3,352,226 - Pf1N1B4_2984 0.84 -1.3 3,352,421 - +0.0 3,352,455 + +0.0 3,352,455 + +0.0 3,352,455 + +0.0 3,352,455 + +0.0 3,352,455 + +0.0 3,352,455 + -2.5 3,352,456 - -1.2 3,352,456 - -1.7 3,352,456 - +0.0 3,352,456 - +0.0 3,352,456 - +0.0 3,352,456 - -1.7 3,352,456 - -1.0 3,352,457 + +0.0 3,352,458 - +0.0 3,352,458 - -1.7 3,352,458 - -1.0 3,352,482 + -3.1 3,352,482 + -2.0 3,352,482 + +0.0 3,352,483 - +0.0 3,352,483 - +0.0 3,352,483 - +0.0 3,352,483 - -1.3 3,352,506 + +0.0 3,352,507 - -1.9 3,352,507 - +0.0 3,352,532 + -1.2 3,352,533 - -2.8 3,352,565 + +0.0 3,352,566 - +0.0 3,352,810 + Pf1N1B4_2985 0.41 +0.0 3,352,811 - Pf1N1B4_2985 0.41 +0.0 3,352,811 - Pf1N1B4_2985 0.41 +0.0 3,353,206 - -2.1 3,353,206 - +0.0 3,353,270 + -1.7 3,353,270 + -1.4 3,353,271 - +0.0 3,353,359 + +0.0 3,353,360 - +0.0 3,353,360 - -1.5 3,353,360 - -2.6 3,353,610 + Pf1N1B4_2986 0.28 +0.0 3,353,610 + Pf1N1B4_2986 0.28 +0.0 3,353,611 - Pf1N1B4_2986 0.28 +0.0 3,353,611 - Pf1N1B4_2986 0.28 +0.0 3,353,739 + Pf1N1B4_2986 0.37 -2.2 3,353,740 - Pf1N1B4_2986 0.37 +0.0 3,353,941 + Pf1N1B4_2986 0.52 +0.0 3,353,941 + Pf1N1B4_2986 0.52 +0.0 3,353,941 + Pf1N1B4_2986 0.52 +0.0 3,353,942 - Pf1N1B4_2986 0.52 +0.0 3,353,942 - Pf1N1B4_2986 0.52 +0.0 3,353,942 - Pf1N1B4_2986 0.52 +0.0 3,354,192 + Pf1N1B4_2986 0.70 +0.0 3,354,215 + Pf1N1B4_2986 0.71 -1.2 3,354,215 + Pf1N1B4_2986 0.71 +0.0 3,354,215 + Pf1N1B4_2986 0.71 +0.0 3,354,215 + Pf1N1B4_2986 0.71 -2.3 3,354,216 - Pf1N1B4_2986 0.71 -2.2 3,354,216 - Pf1N1B4_2986 0.71 -2.4 3,354,243 + Pf1N1B4_2986 0.73 +0.0 3,354,243 + Pf1N1B4_2986 0.73 +0.0 3,354,243 + Pf1N1B4_2986 0.73 +0.0 3,354,244 - Pf1N1B4_2986 0.73 +0.0 3,354,244 - Pf1N1B4_2986 0.73 +0.0 3,354,266 + Pf1N1B4_2986 0.75 -3.2 3,354,267 - Pf1N1B4_2986 0.75 -0.1 3,354,278 + Pf1N1B4_2986 0.76 +0.0 3,354,306 + Pf1N1B4_2986 0.78 -3.2 3,354,306 + Pf1N1B4_2986 0.78 -2.9 3,354,307 - Pf1N1B4_2986 0.78 +0.0 3,354,364 + Pf1N1B4_2986 0.82 +0.0 3,354,385 + Pf1N1B4_2986 0.84 -2.8 3,354,386 - Pf1N1B4_2986 0.84 +0.0 3,354,562 + -0.9 3,354,563 - +0.0 3,354,604 + -1.7 3,354,604 + +0.0 3,354,605 - -1.5 3,354,605 - -1.2 3,354,697 + +0.0 3,354,697 + +0.0 3,354,698 - +0.0 3,354,698 - +0.0 3,354,747 + +0.0 3,354,747 + -3.4 3,354,747 + +0.0 3,354,747 + -1.0 3,354,748 - +1.0 3,354,748 - -1.6 3,354,763 + +0.0 3,354,763 + -2.5 3,354,763 + +0.0 3,354,763 + +0.0 3,354,764 - +0.0 3,354,764 - +0.0 3,354,874 + Pf1N1B4_2987 0.50 +0.0 3,354,874 + Pf1N1B4_2987 0.50 -1.9 3,354,874 + Pf1N1B4_2987 0.50 +0.0 3,354,874 + -2.5 3,354,875 - Pf1N1B4_2987 0.51 -3.3 3,354,875 - Pf1N1B4_2987 0.51 -3.3 3,354,875 - Pf1N1B4_2987 0.51 +0.0 3,354,875 - Pf1N1B4_2987 0.51 +0.0 3,354,937 + Pf1N1B4_2987 0.79 +0.0 3,354,937 + Pf1N1B4_2987 0.79 +0.0 3,354,937 + Pf1N1B4_2987 0.79 +0.0 3,354,938 - Pf1N1B4_2987 0.79 -0.9 3,354,938 - Pf1N1B4_2987 0.79 +0.0 3,354,990 - -2.1 3,355,089 + +0.0 3,355,089 + -2.5 3,355,090 - -1.2 3,355,122 + +0.0 3,355,123 - +0.0 3,355,123 - -1.1 3,355,123 - +0.0 3,355,203 + +0.0 3,355,203 + +0.0 3,355,203 + -1.6 3,355,203 + +0.0 3,355,203 + -0.9 3,355,203 + -1.2 3,355,203 + +0.0 3,355,204 - +0.0 3,355,204 - +0.0 3,355,204 - +0.0 3,355,204 - +0.0 3,355,204 - -0.8 3,355,204 - -1.1 3,355,247 + +0.0 3,355,392 + Pf1N1B4_2988 0.14 +0.0 3,355,392 + Pf1N1B4_2988 0.14 +0.0 3,355,392 + Pf1N1B4_2988 0.14 +0.0 3,355,392 + Pf1N1B4_2988 0.14 +0.0 3,355,393 - Pf1N1B4_2988 0.14 -1.6 3,355,393 - Pf1N1B4_2988 0.14 +0.0 3,355,587 + Pf1N1B4_2988 0.21 -1.6 3,355,588 - Pf1N1B4_2988 0.21 -2.0
Or see this region's nucleotide sequence