Experiment: Nitrate reduction, replicate A t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2744 and Pf1N1B4_2745 are separated by 135 nucleotides Pf1N1B4_2745 and Pf1N1B4_2746 overlap by 11 nucleotides
Pf1N1B4_2744 - Transcriptional regulator, IclR family, at 3,082,630 to 3,083,406
_2744
Pf1N1B4_2745 - membrane protein, putative, at 3,083,542 to 3,084,483
_2745
Pf1N1B4_2746 - Hexuronate transporter, at 3,084,473 to 3,085,810
_2746
Position (kb)
3083
3084
3085 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3082.780 kb on + strand, within Pf1N1B4_2744 at 3082.781 kb on - strand, within Pf1N1B4_2744 at 3082.810 kb on + strand, within Pf1N1B4_2744 at 3082.849 kb on + strand, within Pf1N1B4_2744 at 3082.849 kb on + strand, within Pf1N1B4_2744 at 3082.849 kb on + strand, within Pf1N1B4_2744 at 3082.849 kb on + strand, within Pf1N1B4_2744 at 3082.850 kb on - strand, within Pf1N1B4_2744 at 3083.032 kb on + strand, within Pf1N1B4_2744 at 3083.032 kb on + strand, within Pf1N1B4_2744 at 3083.032 kb on + strand, within Pf1N1B4_2744 at 3083.032 kb on + strand, within Pf1N1B4_2744 at 3083.032 kb on + strand, within Pf1N1B4_2744 at 3083.032 kb on + strand, within Pf1N1B4_2744 at 3083.032 kb on + strand at 3083.032 kb on + strand, within Pf1N1B4_2744 at 3083.033 kb on - strand at 3083.033 kb on - strand, within Pf1N1B4_2744 at 3083.033 kb on - strand, within Pf1N1B4_2744 at 3083.195 kb on - strand, within Pf1N1B4_2744 at 3083.195 kb on - strand, within Pf1N1B4_2744 at 3083.400 kb on + strand at 3083.400 kb on + strand at 3083.400 kb on + strand at 3083.401 kb on - strand at 3083.401 kb on - strand at 3083.439 kb on - strand at 3083.481 kb on - strand at 3083.481 kb on - strand at 3083.482 kb on + strand at 3083.504 kb on - strand at 3083.504 kb on - strand at 3083.554 kb on - strand at 3083.653 kb on + strand, within Pf1N1B4_2745 at 3083.654 kb on - strand, within Pf1N1B4_2745 at 3083.654 kb on - strand, within Pf1N1B4_2745 at 3083.654 kb on - strand, within Pf1N1B4_2745 at 3083.654 kb on - strand, within Pf1N1B4_2745 at 3083.679 kb on + strand, within Pf1N1B4_2745 at 3083.698 kb on + strand, within Pf1N1B4_2745 at 3083.699 kb on - strand at 3083.804 kb on + strand, within Pf1N1B4_2745 at 3083.804 kb on + strand, within Pf1N1B4_2745 at 3083.805 kb on - strand, within Pf1N1B4_2745 at 3084.058 kb on + strand, within Pf1N1B4_2745 at 3084.058 kb on + strand, within Pf1N1B4_2745 at 3084.058 kb on + strand, within Pf1N1B4_2745 at 3084.058 kb on + strand, within Pf1N1B4_2745 at 3084.058 kb on + strand, within Pf1N1B4_2745 at 3084.059 kb on - strand, within Pf1N1B4_2745 at 3084.132 kb on + strand, within Pf1N1B4_2745 at 3084.132 kb on + strand, within Pf1N1B4_2745 at 3084.132 kb on + strand, within Pf1N1B4_2745 at 3084.132 kb on + strand, within Pf1N1B4_2745 at 3084.132 kb on + strand, within Pf1N1B4_2745 at 3084.132 kb on + strand, within Pf1N1B4_2745 at 3084.132 kb on + strand, within Pf1N1B4_2745 at 3084.132 kb on + strand, within Pf1N1B4_2745 at 3084.133 kb on - strand, within Pf1N1B4_2745 at 3084.133 kb on - strand, within Pf1N1B4_2745 at 3084.133 kb on - strand, within Pf1N1B4_2745 at 3084.133 kb on - strand, within Pf1N1B4_2745 at 3084.357 kb on + strand, within Pf1N1B4_2745 at 3084.474 kb on + strand at 3084.487 kb on + strand at 3084.513 kb on + strand at 3084.513 kb on + strand at 3084.513 kb on + strand at 3084.513 kb on + strand at 3084.513 kb on + strand at 3084.513 kb on + strand at 3084.513 kb on + strand at 3084.513 kb on + strand at 3084.513 kb on + strand at 3084.514 kb on - strand at 3084.514 kb on - strand at 3084.514 kb on - strand at 3084.514 kb on - strand at 3084.514 kb on - strand at 3084.514 kb on - strand at 3084.514 kb on - strand at 3084.514 kb on - strand at 3084.537 kb on + strand at 3084.537 kb on + strand at 3084.537 kb on + strand at 3084.537 kb on + strand at 3084.538 kb on - strand at 3084.538 kb on - strand at 3084.538 kb on - strand at 3084.551 kb on + strand at 3084.551 kb on + strand at 3084.552 kb on - strand at 3084.552 kb on - strand at 3084.552 kb on - strand at 3084.552 kb on - strand at 3084.563 kb on + strand at 3084.570 kb on + strand at 3084.570 kb on + strand at 3084.570 kb on + strand at 3084.570 kb on + strand at 3084.570 kb on + strand at 3084.570 kb on + strand at 3084.570 kb on + strand at 3084.570 kb on + strand at 3084.571 kb on - strand at 3084.571 kb on - strand at 3084.571 kb on - strand at 3084.571 kb on - strand at 3084.571 kb on - strand at 3084.571 kb on - strand at 3084.571 kb on - strand at 3084.571 kb on - strand at 3084.571 kb on - strand at 3084.687 kb on + strand, within Pf1N1B4_2746 at 3084.688 kb on - strand, within Pf1N1B4_2746 at 3084.688 kb on - strand, within Pf1N1B4_2746 at 3084.924 kb on + strand at 3084.925 kb on - strand, within Pf1N1B4_2746 at 3084.925 kb on - strand, within Pf1N1B4_2746 at 3084.925 kb on - strand, within Pf1N1B4_2746 at 3084.943 kb on + strand, within Pf1N1B4_2746 at 3084.944 kb on - strand, within Pf1N1B4_2746 at 3084.975 kb on + strand, within Pf1N1B4_2746 at 3084.975 kb on + strand, within Pf1N1B4_2746 at 3084.975 kb on + strand, within Pf1N1B4_2746 at 3084.975 kb on + strand, within Pf1N1B4_2746 at 3084.976 kb on - strand, within Pf1N1B4_2746 at 3084.976 kb on - strand, within Pf1N1B4_2746 at 3084.976 kb on - strand, within Pf1N1B4_2746 at 3084.976 kb on - strand, within Pf1N1B4_2746 at 3084.976 kb on - strand, within Pf1N1B4_2746 at 3085.005 kb on + strand, within Pf1N1B4_2746 at 3085.006 kb on - strand, within Pf1N1B4_2746 at 3085.006 kb on - strand, within Pf1N1B4_2746 at 3085.006 kb on - strand, within Pf1N1B4_2746 at 3085.011 kb on + strand, within Pf1N1B4_2746 at 3085.011 kb on + strand, within Pf1N1B4_2746 at 3085.011 kb on + strand, within Pf1N1B4_2746 at 3085.011 kb on + strand, within Pf1N1B4_2746 at 3085.011 kb on + strand, within Pf1N1B4_2746 at 3085.012 kb on - strand, within Pf1N1B4_2746 at 3085.012 kb on - strand, within Pf1N1B4_2746 at 3085.012 kb on - strand, within Pf1N1B4_2746 at 3085.012 kb on - strand, within Pf1N1B4_2746 at 3085.012 kb on - strand, within Pf1N1B4_2746 at 3085.012 kb on - strand, within Pf1N1B4_2746 at 3085.131 kb on + strand, within Pf1N1B4_2746 at 3085.131 kb on + strand, within Pf1N1B4_2746 at 3085.132 kb on - strand, within Pf1N1B4_2746 at 3085.132 kb on - strand, within Pf1N1B4_2746 at 3085.132 kb on - strand, within Pf1N1B4_2746 at 3085.179 kb on + strand, within Pf1N1B4_2746 at 3085.430 kb on - strand, within Pf1N1B4_2746
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1 remove 3,082,780 + Pf1N1B4_2744 0.19 +0.0 3,082,781 - Pf1N1B4_2744 0.19 +0.2 3,082,810 + Pf1N1B4_2744 0.23 +0.0 3,082,849 + Pf1N1B4_2744 0.28 +0.0 3,082,849 + Pf1N1B4_2744 0.28 +0.0 3,082,849 + Pf1N1B4_2744 0.28 -1.0 3,082,849 + Pf1N1B4_2744 0.28 +0.0 3,082,850 - Pf1N1B4_2744 0.28 +0.0 3,083,032 + Pf1N1B4_2744 0.52 +0.0 3,083,032 + Pf1N1B4_2744 0.52 -1.3 3,083,032 + Pf1N1B4_2744 0.52 +0.0 3,083,032 + Pf1N1B4_2744 0.52 -2.0 3,083,032 + Pf1N1B4_2744 0.52 -1.4 3,083,032 + Pf1N1B4_2744 0.52 +0.0 3,083,032 + -1.1 3,083,032 + Pf1N1B4_2744 0.52 +0.0 3,083,033 - -1.2 3,083,033 - Pf1N1B4_2744 0.52 +0.0 3,083,033 - Pf1N1B4_2744 0.52 +0.0 3,083,195 - Pf1N1B4_2744 0.73 -0.8 3,083,195 - Pf1N1B4_2744 0.73 +0.0 3,083,400 + +0.0 3,083,400 + +0.0 3,083,400 + +0.0 3,083,401 - -1.7 3,083,401 - -1.2 3,083,439 - +0.0 3,083,481 - +0.0 3,083,481 - -0.7 3,083,482 + +0.0 3,083,504 - +0.0 3,083,504 - +0.0 3,083,554 - -0.2 3,083,653 + Pf1N1B4_2745 0.12 -1.6 3,083,654 - Pf1N1B4_2745 0.12 -0.5 3,083,654 - Pf1N1B4_2745 0.12 +0.0 3,083,654 - Pf1N1B4_2745 0.12 +0.0 3,083,654 - Pf1N1B4_2745 0.12 +0.0 3,083,679 + Pf1N1B4_2745 0.15 +0.0 3,083,698 + Pf1N1B4_2745 0.17 +0.0 3,083,699 - +0.0 3,083,804 + Pf1N1B4_2745 0.28 -2.6 3,083,804 + Pf1N1B4_2745 0.28 +0.0 3,083,805 - Pf1N1B4_2745 0.28 +0.0 3,084,058 + Pf1N1B4_2745 0.55 +0.0 3,084,058 + Pf1N1B4_2745 0.55 -1.4 3,084,058 + Pf1N1B4_2745 0.55 +0.0 3,084,058 + Pf1N1B4_2745 0.55 +0.0 3,084,058 + Pf1N1B4_2745 0.55 -2.3 3,084,059 - Pf1N1B4_2745 0.55 -3.8 3,084,132 + Pf1N1B4_2745 0.63 -1.3 3,084,132 + Pf1N1B4_2745 0.63 +0.0 3,084,132 + Pf1N1B4_2745 0.63 +0.0 3,084,132 + Pf1N1B4_2745 0.63 +0.0 3,084,132 + Pf1N1B4_2745 0.63 +0.0 3,084,132 + Pf1N1B4_2745 0.63 +0.0 3,084,132 + Pf1N1B4_2745 0.63 +0.0 3,084,132 + Pf1N1B4_2745 0.63 +0.0 3,084,133 - Pf1N1B4_2745 0.63 +0.0 3,084,133 - Pf1N1B4_2745 0.63 +0.0 3,084,133 - Pf1N1B4_2745 0.63 +0.0 3,084,133 - Pf1N1B4_2745 0.63 +0.0 3,084,357 + Pf1N1B4_2745 0.87 -1.9 3,084,474 + +0.0 3,084,487 + -1.1 3,084,513 + +0.0 3,084,513 + +0.0 3,084,513 + -1.3 3,084,513 + -1.9 3,084,513 + +1.6 3,084,513 + +0.0 3,084,513 + +0.0 3,084,513 + +0.0 3,084,513 + -2.5 3,084,514 - -1.1 3,084,514 - -1.3 3,084,514 - -1.2 3,084,514 - -1.6 3,084,514 - +0.0 3,084,514 - +0.0 3,084,514 - -1.0 3,084,514 - +0.0 3,084,537 + +0.0 3,084,537 + +0.0 3,084,537 + +0.0 3,084,537 + +0.0 3,084,538 - +0.0 3,084,538 - +0.0 3,084,538 - -2.4 3,084,551 + -1.6 3,084,551 + +0.0 3,084,552 - -1.8 3,084,552 - +0.0 3,084,552 - +0.0 3,084,552 - +0.0 3,084,563 + +0.0 3,084,570 + +0.0 3,084,570 + -0.8 3,084,570 + +0.0 3,084,570 + +0.0 3,084,570 + -1.4 3,084,570 + -1.2 3,084,570 + -0.4 3,084,570 + +0.0 3,084,571 - +0.0 3,084,571 - +0.0 3,084,571 - +0.0 3,084,571 - +0.0 3,084,571 - -3.2 3,084,571 - +0.0 3,084,571 - +0.0 3,084,571 - -0.6 3,084,571 - +0.0 3,084,687 + Pf1N1B4_2746 0.16 -1.8 3,084,688 - Pf1N1B4_2746 0.16 +0.0 3,084,688 - Pf1N1B4_2746 0.16 +0.0 3,084,924 + -1.3 3,084,925 - Pf1N1B4_2746 0.34 +0.0 3,084,925 - Pf1N1B4_2746 0.34 +0.0 3,084,925 - Pf1N1B4_2746 0.34 +0.0 3,084,943 + Pf1N1B4_2746 0.35 -1.6 3,084,944 - Pf1N1B4_2746 0.35 +0.0 3,084,975 + Pf1N1B4_2746 0.38 +0.0 3,084,975 + Pf1N1B4_2746 0.38 -2.8 3,084,975 + Pf1N1B4_2746 0.38 +0.0 3,084,975 + Pf1N1B4_2746 0.38 -2.6 3,084,976 - Pf1N1B4_2746 0.38 +0.0 3,084,976 - Pf1N1B4_2746 0.38 -3.2 3,084,976 - Pf1N1B4_2746 0.38 -2.5 3,084,976 - Pf1N1B4_2746 0.38 +0.0 3,084,976 - Pf1N1B4_2746 0.38 -1.2 3,085,005 + Pf1N1B4_2746 0.40 +0.0 3,085,006 - Pf1N1B4_2746 0.40 +0.0 3,085,006 - Pf1N1B4_2746 0.40 -2.0 3,085,006 - Pf1N1B4_2746 0.40 +0.0 3,085,011 + Pf1N1B4_2746 0.40 +0.0 3,085,011 + Pf1N1B4_2746 0.40 +0.0 3,085,011 + Pf1N1B4_2746 0.40 -2.0 3,085,011 + Pf1N1B4_2746 0.40 +0.0 3,085,011 + Pf1N1B4_2746 0.40 +0.0 3,085,012 - Pf1N1B4_2746 0.40 -1.7 3,085,012 - Pf1N1B4_2746 0.40 -1.7 3,085,012 - Pf1N1B4_2746 0.40 +0.0 3,085,012 - Pf1N1B4_2746 0.40 -3.7 3,085,012 - Pf1N1B4_2746 0.40 -1.5 3,085,012 - Pf1N1B4_2746 0.40 -1.9 3,085,131 + Pf1N1B4_2746 0.49 +0.0 3,085,131 + Pf1N1B4_2746 0.49 +0.0 3,085,132 - Pf1N1B4_2746 0.49 +0.0 3,085,132 - Pf1N1B4_2746 0.49 +0.0 3,085,132 - Pf1N1B4_2746 0.49 +0.0 3,085,179 + Pf1N1B4_2746 0.53 +0.0 3,085,430 - Pf1N1B4_2746 0.72 +0.0
Or see this region's nucleotide sequence