Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2744

Experiment: Nitrate reduction, replicate A t1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2743 and Pf1N1B4_2744 are separated by 235 nucleotidesPf1N1B4_2744 and Pf1N1B4_2745 are separated by 135 nucleotides Pf1N1B4_2743 - YpfJ protein, zinc metalloprotease superfamily, at 3,081,501 to 3,082,394 _2743 Pf1N1B4_2744 - Transcriptional regulator, IclR family, at 3,082,630 to 3,083,406 _2744 Pf1N1B4_2745 - membrane protein, putative, at 3,083,542 to 3,084,483 _2745 Position (kb) 3082 3083 3084Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3081.882 kb on + strand, within Pf1N1B4_2743at 3081.883 kb on - strand, within Pf1N1B4_2743at 3081.883 kb on - strand, within Pf1N1B4_2743at 3081.883 kb on - strand, within Pf1N1B4_2743at 3081.883 kb on - strand, within Pf1N1B4_2743at 3081.883 kb on - strand, within Pf1N1B4_2743at 3081.886 kb on + strand, within Pf1N1B4_2743at 3081.887 kb on - strand, within Pf1N1B4_2743at 3081.887 kb on - strandat 3081.887 kb on - strand, within Pf1N1B4_2743at 3081.955 kb on + strand, within Pf1N1B4_2743at 3081.955 kb on + strand, within Pf1N1B4_2743at 3081.955 kb on + strand, within Pf1N1B4_2743at 3081.955 kb on + strand, within Pf1N1B4_2743at 3081.955 kb on + strand, within Pf1N1B4_2743at 3081.956 kb on - strand, within Pf1N1B4_2743at 3081.956 kb on - strand, within Pf1N1B4_2743at 3081.956 kb on - strand, within Pf1N1B4_2743at 3081.979 kb on + strand, within Pf1N1B4_2743at 3081.979 kb on + strand, within Pf1N1B4_2743at 3081.979 kb on + strand, within Pf1N1B4_2743at 3081.979 kb on + strand, within Pf1N1B4_2743at 3081.979 kb on + strand, within Pf1N1B4_2743at 3081.979 kb on + strand, within Pf1N1B4_2743at 3081.980 kb on - strand, within Pf1N1B4_2743at 3082.058 kb on + strand, within Pf1N1B4_2743at 3082.058 kb on + strand, within Pf1N1B4_2743at 3082.058 kb on + strand, within Pf1N1B4_2743at 3082.059 kb on - strand, within Pf1N1B4_2743at 3082.059 kb on - strand, within Pf1N1B4_2743at 3082.059 kb on - strand, within Pf1N1B4_2743at 3082.059 kb on - strand, within Pf1N1B4_2743at 3082.059 kb on - strand, within Pf1N1B4_2743at 3082.059 kb on - strand, within Pf1N1B4_2743at 3082.059 kb on - strand, within Pf1N1B4_2743at 3082.059 kb on - strand, within Pf1N1B4_2743at 3082.059 kb on - strand, within Pf1N1B4_2743at 3082.059 kb on - strand, within Pf1N1B4_2743at 3082.059 kb on - strand, within Pf1N1B4_2743at 3082.059 kb on - strand, within Pf1N1B4_2743at 3082.061 kb on - strand, within Pf1N1B4_2743at 3082.061 kb on - strand, within Pf1N1B4_2743at 3082.451 kb on + strandat 3082.451 kb on + strandat 3082.452 kb on - strandat 3082.452 kb on - strandat 3082.452 kb on - strandat 3082.452 kb on - strandat 3082.452 kb on - strandat 3082.452 kb on - strandat 3082.452 kb on - strandat 3082.452 kb on - strandat 3082.455 kb on + strandat 3082.456 kb on - strandat 3082.456 kb on - strandat 3082.456 kb on - strandat 3082.456 kb on - strandat 3082.505 kb on - strandat 3082.505 kb on - strandat 3082.780 kb on + strand, within Pf1N1B4_2744at 3082.781 kb on - strand, within Pf1N1B4_2744at 3082.810 kb on + strand, within Pf1N1B4_2744at 3082.849 kb on + strand, within Pf1N1B4_2744at 3082.849 kb on + strand, within Pf1N1B4_2744at 3082.849 kb on + strand, within Pf1N1B4_2744at 3082.849 kb on + strand, within Pf1N1B4_2744at 3082.850 kb on - strand, within Pf1N1B4_2744at 3083.032 kb on + strand, within Pf1N1B4_2744at 3083.032 kb on + strand, within Pf1N1B4_2744at 3083.032 kb on + strand, within Pf1N1B4_2744at 3083.032 kb on + strand, within Pf1N1B4_2744at 3083.032 kb on + strand, within Pf1N1B4_2744at 3083.032 kb on + strand, within Pf1N1B4_2744at 3083.032 kb on + strandat 3083.032 kb on + strand, within Pf1N1B4_2744at 3083.033 kb on - strandat 3083.033 kb on - strand, within Pf1N1B4_2744at 3083.033 kb on - strand, within Pf1N1B4_2744at 3083.195 kb on - strand, within Pf1N1B4_2744at 3083.195 kb on - strand, within Pf1N1B4_2744at 3083.400 kb on + strandat 3083.400 kb on + strandat 3083.400 kb on + strandat 3083.401 kb on - strandat 3083.401 kb on - strandat 3083.439 kb on - strandat 3083.481 kb on - strandat 3083.481 kb on - strandat 3083.482 kb on + strandat 3083.504 kb on - strandat 3083.504 kb on - strandat 3083.554 kb on - strandat 3083.653 kb on + strand, within Pf1N1B4_2745at 3083.654 kb on - strand, within Pf1N1B4_2745at 3083.654 kb on - strand, within Pf1N1B4_2745at 3083.654 kb on - strand, within Pf1N1B4_2745at 3083.654 kb on - strand, within Pf1N1B4_2745at 3083.679 kb on + strand, within Pf1N1B4_2745at 3083.698 kb on + strand, within Pf1N1B4_2745at 3083.699 kb on - strandat 3083.804 kb on + strand, within Pf1N1B4_2745at 3083.804 kb on + strand, within Pf1N1B4_2745at 3083.805 kb on - strand, within Pf1N1B4_2745at 3084.058 kb on + strand, within Pf1N1B4_2745at 3084.058 kb on + strand, within Pf1N1B4_2745at 3084.058 kb on + strand, within Pf1N1B4_2745at 3084.058 kb on + strand, within Pf1N1B4_2745at 3084.058 kb on + strand, within Pf1N1B4_2745at 3084.059 kb on - strand, within Pf1N1B4_2745at 3084.132 kb on + strand, within Pf1N1B4_2745at 3084.132 kb on + strand, within Pf1N1B4_2745at 3084.132 kb on + strand, within Pf1N1B4_2745at 3084.132 kb on + strand, within Pf1N1B4_2745at 3084.132 kb on + strand, within Pf1N1B4_2745at 3084.132 kb on + strand, within Pf1N1B4_2745at 3084.132 kb on + strand, within Pf1N1B4_2745at 3084.132 kb on + strand, within Pf1N1B4_2745at 3084.133 kb on - strand, within Pf1N1B4_2745at 3084.133 kb on - strand, within Pf1N1B4_2745at 3084.133 kb on - strand, within Pf1N1B4_2745at 3084.133 kb on - strand, within Pf1N1B4_2745at 3084.357 kb on + strand, within Pf1N1B4_2745

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1
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3,081,882 + Pf1N1B4_2743 0.43 +0.0
3,081,883 - Pf1N1B4_2743 0.43 -0.8
3,081,883 - Pf1N1B4_2743 0.43 -0.6
3,081,883 - Pf1N1B4_2743 0.43 +0.0
3,081,883 - Pf1N1B4_2743 0.43 +0.0
3,081,883 - Pf1N1B4_2743 0.43 +0.0
3,081,886 + Pf1N1B4_2743 0.43 +0.0
3,081,887 - Pf1N1B4_2743 0.43 +0.0
3,081,887 - -2.1
3,081,887 - Pf1N1B4_2743 0.43 +0.0
3,081,955 + Pf1N1B4_2743 0.51 +0.0
3,081,955 + Pf1N1B4_2743 0.51 +0.0
3,081,955 + Pf1N1B4_2743 0.51 +0.0
3,081,955 + Pf1N1B4_2743 0.51 +0.0
3,081,955 + Pf1N1B4_2743 0.51 +0.0
3,081,956 - Pf1N1B4_2743 0.51 +0.0
3,081,956 - Pf1N1B4_2743 0.51 +0.0
3,081,956 - Pf1N1B4_2743 0.51 +0.0
3,081,979 + Pf1N1B4_2743 0.53 +0.0
3,081,979 + Pf1N1B4_2743 0.53 -0.9
3,081,979 + Pf1N1B4_2743 0.53 -0.4
3,081,979 + Pf1N1B4_2743 0.53 +0.0
3,081,979 + Pf1N1B4_2743 0.53 +0.0
3,081,979 + Pf1N1B4_2743 0.53 +0.0
3,081,980 - Pf1N1B4_2743 0.54 -4.5
3,082,058 + Pf1N1B4_2743 0.62 +0.0
3,082,058 + Pf1N1B4_2743 0.62 +0.0
3,082,058 + Pf1N1B4_2743 0.62 +0.0
3,082,059 - Pf1N1B4_2743 0.62 +0.0
3,082,059 - Pf1N1B4_2743 0.62 +0.0
3,082,059 - Pf1N1B4_2743 0.62 -0.9
3,082,059 - Pf1N1B4_2743 0.62 +0.0
3,082,059 - Pf1N1B4_2743 0.62 -1.0
3,082,059 - Pf1N1B4_2743 0.62 +0.0
3,082,059 - Pf1N1B4_2743 0.62 +0.0
3,082,059 - Pf1N1B4_2743 0.62 +0.0
3,082,059 - Pf1N1B4_2743 0.62 -1.1
3,082,059 - Pf1N1B4_2743 0.62 +0.0
3,082,059 - Pf1N1B4_2743 0.62 +0.0
3,082,059 - Pf1N1B4_2743 0.62 -1.1
3,082,061 - Pf1N1B4_2743 0.63 +0.0
3,082,061 - Pf1N1B4_2743 0.63 +0.0
3,082,451 + -1.9
3,082,451 + -1.6
3,082,452 - +0.0
3,082,452 - -1.6
3,082,452 - -1.5
3,082,452 - +0.0
3,082,452 - +0.0
3,082,452 - +0.0
3,082,452 - +0.0
3,082,452 - +0.0
3,082,455 + +0.0
3,082,456 - +0.0
3,082,456 - -2.3
3,082,456 - +0.0
3,082,456 - +0.0
3,082,505 - +0.0
3,082,505 - -1.6
3,082,780 + Pf1N1B4_2744 0.19 +0.0
3,082,781 - Pf1N1B4_2744 0.19 +0.2
3,082,810 + Pf1N1B4_2744 0.23 +0.0
3,082,849 + Pf1N1B4_2744 0.28 +0.0
3,082,849 + Pf1N1B4_2744 0.28 +0.0
3,082,849 + Pf1N1B4_2744 0.28 -1.0
3,082,849 + Pf1N1B4_2744 0.28 +0.0
3,082,850 - Pf1N1B4_2744 0.28 +0.0
3,083,032 + Pf1N1B4_2744 0.52 +0.0
3,083,032 + Pf1N1B4_2744 0.52 -1.3
3,083,032 + Pf1N1B4_2744 0.52 +0.0
3,083,032 + Pf1N1B4_2744 0.52 -2.0
3,083,032 + Pf1N1B4_2744 0.52 -1.4
3,083,032 + Pf1N1B4_2744 0.52 +0.0
3,083,032 + -1.1
3,083,032 + Pf1N1B4_2744 0.52 +0.0
3,083,033 - -1.2
3,083,033 - Pf1N1B4_2744 0.52 +0.0
3,083,033 - Pf1N1B4_2744 0.52 +0.0
3,083,195 - Pf1N1B4_2744 0.73 -0.8
3,083,195 - Pf1N1B4_2744 0.73 +0.0
3,083,400 + +0.0
3,083,400 + +0.0
3,083,400 + +0.0
3,083,401 - -1.7
3,083,401 - -1.2
3,083,439 - +0.0
3,083,481 - +0.0
3,083,481 - -0.7
3,083,482 + +0.0
3,083,504 - +0.0
3,083,504 - +0.0
3,083,554 - -0.2
3,083,653 + Pf1N1B4_2745 0.12 -1.6
3,083,654 - Pf1N1B4_2745 0.12 -0.5
3,083,654 - Pf1N1B4_2745 0.12 +0.0
3,083,654 - Pf1N1B4_2745 0.12 +0.0
3,083,654 - Pf1N1B4_2745 0.12 +0.0
3,083,679 + Pf1N1B4_2745 0.15 +0.0
3,083,698 + Pf1N1B4_2745 0.17 +0.0
3,083,699 - +0.0
3,083,804 + Pf1N1B4_2745 0.28 -2.6
3,083,804 + Pf1N1B4_2745 0.28 +0.0
3,083,805 - Pf1N1B4_2745 0.28 +0.0
3,084,058 + Pf1N1B4_2745 0.55 +0.0
3,084,058 + Pf1N1B4_2745 0.55 -1.4
3,084,058 + Pf1N1B4_2745 0.55 +0.0
3,084,058 + Pf1N1B4_2745 0.55 +0.0
3,084,058 + Pf1N1B4_2745 0.55 -2.3
3,084,059 - Pf1N1B4_2745 0.55 -3.8
3,084,132 + Pf1N1B4_2745 0.63 -1.3
3,084,132 + Pf1N1B4_2745 0.63 +0.0
3,084,132 + Pf1N1B4_2745 0.63 +0.0
3,084,132 + Pf1N1B4_2745 0.63 +0.0
3,084,132 + Pf1N1B4_2745 0.63 +0.0
3,084,132 + Pf1N1B4_2745 0.63 +0.0
3,084,132 + Pf1N1B4_2745 0.63 +0.0
3,084,132 + Pf1N1B4_2745 0.63 +0.0
3,084,133 - Pf1N1B4_2745 0.63 +0.0
3,084,133 - Pf1N1B4_2745 0.63 +0.0
3,084,133 - Pf1N1B4_2745 0.63 +0.0
3,084,133 - Pf1N1B4_2745 0.63 +0.0
3,084,357 + Pf1N1B4_2745 0.87 -1.9

Or see this region's nucleotide sequence