Experiment: Nitrate reduction, replicate A t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_26 and Pf1N1B4_27 are separated by 337 nucleotides Pf1N1B4_27 and Pf1N1B4_28 are separated by 235 nucleotides Pf1N1B4_28 and Pf1N1B4_29 overlap by 4 nucleotides Pf1N1B4_29 and Pf1N1B4_5164 are separated by 225 nucleotides
Pf1N1B4_26 - Excinuclease ABC subunit B, at 28,200 to 30,215
_26
Pf1N1B4_27 - Biosynthetic Aromatic amino acid aminotransferase alpha (EC 2.6.1.57) @ Aromatic-amino-acid aminotransferase (EC 2.6.1.57), at 30,553 to 31,749
_27
Pf1N1B4_28 - FIG00958186: hypothetical protein, at 31,985 to 32,185
_28
Pf1N1B4_29 - FIG00967200: hypothetical protein, at 32,182 to 32,412
_29
Pf1N1B4_5164 - tRNA-Asn-GTT, at 32,638 to 32,710
_5164
Position (kb)
30
31
32 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 29.616 kb on - strand at 29.677 kb on + strand, within Pf1N1B4_26 at 29.677 kb on + strand, within Pf1N1B4_26 at 29.677 kb on + strand, within Pf1N1B4_26 at 29.678 kb on - strand, within Pf1N1B4_26 at 29.758 kb on + strand, within Pf1N1B4_26 at 29.759 kb on - strand, within Pf1N1B4_26 at 29.782 kb on + strand, within Pf1N1B4_26 at 29.918 kb on - strand, within Pf1N1B4_26 at 29.936 kb on - strand, within Pf1N1B4_26 at 29.944 kb on + strand, within Pf1N1B4_26 at 29.945 kb on - strand, within Pf1N1B4_26 at 29.957 kb on - strand, within Pf1N1B4_26 at 29.998 kb on + strand, within Pf1N1B4_26 at 29.999 kb on - strand, within Pf1N1B4_26 at 29.999 kb on - strand, within Pf1N1B4_26 at 30.198 kb on + strand at 30.215 kb on + strand at 30.215 kb on + strand at 30.216 kb on - strand at 30.216 kb on - strand at 30.216 kb on - strand at 30.216 kb on - strand at 30.216 kb on - strand at 30.216 kb on - strand at 30.216 kb on - strand at 30.216 kb on - strand at 30.216 kb on - strand at 30.219 kb on + strand at 30.219 kb on + strand at 30.219 kb on + strand at 30.220 kb on - strand at 30.220 kb on - strand at 30.220 kb on - strand at 30.220 kb on - strand at 30.220 kb on - strand at 30.220 kb on - strand at 30.220 kb on - strand at 30.220 kb on - strand at 30.220 kb on - strand at 30.355 kb on - strand at 30.464 kb on + strand at 30.465 kb on - strand at 30.466 kb on + strand at 30.467 kb on - strand at 30.467 kb on - strand at 30.467 kb on - strand at 30.467 kb on - strand at 30.469 kb on + strand at 30.469 kb on + strand at 30.469 kb on + strand at 30.469 kb on + strand at 30.469 kb on + strand at 30.469 kb on + strand at 30.469 kb on + strand at 30.470 kb on - strand at 30.494 kb on - strand at 30.551 kb on + strand at 30.664 kb on + strand at 30.778 kb on + strand, within Pf1N1B4_27 at 31.006 kb on + strand, within Pf1N1B4_27 at 31.007 kb on - strand at 31.405 kb on + strand, within Pf1N1B4_27 at 31.405 kb on + strand, within Pf1N1B4_27 at 31.405 kb on + strand, within Pf1N1B4_27 at 31.405 kb on + strand, within Pf1N1B4_27 at 31.406 kb on - strand, within Pf1N1B4_27 at 31.606 kb on + strand, within Pf1N1B4_27 at 31.631 kb on + strand at 31.631 kb on + strand at 31.660 kb on + strand at 31.660 kb on + strand at 31.660 kb on + strand at 31.660 kb on + strand at 31.660 kb on + strand at 31.661 kb on - strand at 31.661 kb on - strand at 31.661 kb on - strand at 31.661 kb on - strand at 31.762 kb on + strand at 31.781 kb on - strand at 31.797 kb on + strand at 31.798 kb on - strand at 31.810 kb on + strand at 31.892 kb on - strand at 31.952 kb on + strand at 31.952 kb on + strand at 31.958 kb on + strand at 31.958 kb on + strand at 31.959 kb on - strand at 31.959 kb on - strand at 31.959 kb on - strand at 31.959 kb on - strand at 31.959 kb on - strand at 32.001 kb on + strand at 32.002 kb on - strand at 32.144 kb on + strand, within Pf1N1B4_28 at 32.170 kb on + strand at 32.171 kb on - strand at 32.171 kb on - strand at 32.223 kb on - strand, within Pf1N1B4_29 at 32.223 kb on - strand, within Pf1N1B4_29 at 32.232 kb on - strand, within Pf1N1B4_29 at 32.232 kb on - strand, within Pf1N1B4_29 at 32.232 kb on - strand, within Pf1N1B4_29 at 32.463 kb on + strand at 32.517 kb on + strand at 32.526 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1 remove 29,616 - +0.0 29,677 + Pf1N1B4_26 0.73 -0.7 29,677 + Pf1N1B4_26 0.73 -2.1 29,677 + Pf1N1B4_26 0.73 +0.0 29,678 - Pf1N1B4_26 0.73 +0.0 29,758 + Pf1N1B4_26 0.77 -1.8 29,759 - Pf1N1B4_26 0.77 +0.0 29,782 + Pf1N1B4_26 0.78 -3.9 29,918 - Pf1N1B4_26 0.85 -0.5 29,936 - Pf1N1B4_26 0.86 -2.1 29,944 + Pf1N1B4_26 0.87 -1.9 29,945 - Pf1N1B4_26 0.87 +0.0 29,957 - Pf1N1B4_26 0.87 +0.6 29,998 + Pf1N1B4_26 0.89 +0.0 29,999 - Pf1N1B4_26 0.89 +0.0 29,999 - Pf1N1B4_26 0.89 -1.8 30,198 + -1.0 30,215 + -1.9 30,215 + +0.0 30,216 - -2.0 30,216 - -2.4 30,216 - -2.2 30,216 - +0.0 30,216 - +0.0 30,216 - +0.0 30,216 - -1.1 30,216 - +0.0 30,216 - -2.0 30,219 + -2.2 30,219 + +0.0 30,219 + -0.5 30,220 - -1.0 30,220 - -0.6 30,220 - +0.0 30,220 - +0.0 30,220 - +0.0 30,220 - +0.0 30,220 - +0.0 30,220 - -2.4 30,220 - +0.0 30,355 - +0.0 30,464 + -1.0 30,465 - -1.1 30,466 + -1.4 30,467 - +0.0 30,467 - +0.0 30,467 - -2.5 30,467 - +0.0 30,469 + +0.0 30,469 + +0.0 30,469 + +0.0 30,469 + +0.0 30,469 + -1.8 30,469 + -1.9 30,469 + -1.6 30,470 - -0.7 30,494 - +0.0 30,551 + +0.0 30,664 + -3.2 30,778 + Pf1N1B4_27 0.19 +0.0 31,006 + Pf1N1B4_27 0.38 +0.0 31,007 - -2.1 31,405 + Pf1N1B4_27 0.71 -3.4 31,405 + Pf1N1B4_27 0.71 +0.0 31,405 + Pf1N1B4_27 0.71 +0.0 31,405 + Pf1N1B4_27 0.71 -2.4 31,406 - Pf1N1B4_27 0.71 +0.0 31,606 + Pf1N1B4_27 0.88 +0.0 31,631 + +0.0 31,631 + +0.0 31,660 + +0.0 31,660 + -2.9 31,660 + -2.6 31,660 + +0.0 31,660 + +0.0 31,661 - +0.0 31,661 - +0.0 31,661 - -2.4 31,661 - +0.0 31,762 + +0.0 31,781 - +0.0 31,797 + -1.9 31,798 - -1.0 31,810 + +0.0 31,892 - +0.0 31,952 + -2.7 31,952 + -1.5 31,958 + +0.0 31,958 + -1.3 31,959 - -3.0 31,959 - -2.0 31,959 - -2.0 31,959 - -2.4 31,959 - +0.0 32,001 + +0.0 32,002 - -4.0 32,144 + Pf1N1B4_28 0.79 +0.0 32,170 + +0.0 32,171 - -2.2 32,171 - +0.0 32,223 - Pf1N1B4_29 0.18 -1.9 32,223 - Pf1N1B4_29 0.18 -1.5 32,232 - Pf1N1B4_29 0.22 -2.3 32,232 - Pf1N1B4_29 0.22 +0.0 32,232 - Pf1N1B4_29 0.22 +0.0 32,463 + -2.4 32,517 + +0.0 32,526 - -2.5
Or see this region's nucleotide sequence