Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_257

Experiment: Nitrate reduction, replicate A t1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_256 and Pf1N1B4_257 are separated by 111 nucleotidesPf1N1B4_257 and Pf1N1B4_258 overlap by 17 nucleotidesPf1N1B4_258 and Pf1N1B4_259 overlap by 4 nucleotides Pf1N1B4_255 - Free methionine-(R)-sulfoxide reductase, contains GAF domain, at 314,997 to 315,479 _255 Pf1N1B4_256 - putative ATP/GTP-binding protein, at 315,476 to 316,366 _256 Pf1N1B4_257 - sensor histidine kinase/response regulator, at 316,478 to 318,841 _257 Pf1N1B4_258 - Organic hydroperoxide resistance transcriptional regulator, at 318,825 to 319,286 _258 Pf1N1B4_259 - Glutathione peroxidase family protein, at 319,283 to 319,768 _259 Position (kb) 316 317 318 319Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 315.529 kb on - strandat 315.685 kb on - strand, within Pf1N1B4_256at 315.685 kb on - strand, within Pf1N1B4_256at 315.685 kb on - strand, within Pf1N1B4_256at 315.832 kb on - strand, within Pf1N1B4_256at 315.832 kb on - strand, within Pf1N1B4_256at 315.832 kb on - strand, within Pf1N1B4_256at 315.844 kb on + strand, within Pf1N1B4_256at 315.844 kb on + strand, within Pf1N1B4_256at 316.392 kb on + strandat 316.392 kb on + strandat 316.392 kb on + strandat 316.393 kb on - strandat 316.393 kb on - strandat 316.393 kb on - strandat 316.393 kb on - strandat 316.393 kb on - strandat 316.393 kb on - strandat 316.427 kb on - strandat 316.434 kb on + strandat 316.434 kb on + strandat 316.435 kb on - strandat 316.435 kb on - strandat 316.477 kb on + strandat 316.477 kb on + strandat 316.477 kb on + strandat 316.477 kb on + strandat 316.478 kb on - strandat 316.551 kb on - strandat 316.551 kb on - strandat 316.551 kb on - strandat 316.551 kb on - strandat 316.618 kb on - strandat 316.618 kb on - strandat 316.628 kb on + strandat 316.629 kb on - strandat 316.662 kb on + strandat 316.663 kb on - strandat 316.663 kb on - strandat 316.901 kb on + strand, within Pf1N1B4_257at 316.958 kb on + strand, within Pf1N1B4_257at 316.958 kb on + strand, within Pf1N1B4_257at 316.959 kb on - strandat 316.959 kb on - strand, within Pf1N1B4_257at 316.959 kb on - strand, within Pf1N1B4_257at 316.959 kb on - strand, within Pf1N1B4_257at 316.959 kb on - strand, within Pf1N1B4_257at 316.959 kb on - strand, within Pf1N1B4_257at 316.959 kb on - strand, within Pf1N1B4_257at 316.959 kb on - strand, within Pf1N1B4_257at 316.959 kb on - strand, within Pf1N1B4_257at 317.057 kb on + strand, within Pf1N1B4_257at 317.058 kb on - strandat 317.058 kb on - strand, within Pf1N1B4_257at 317.214 kb on + strand, within Pf1N1B4_257at 317.215 kb on - strand, within Pf1N1B4_257at 317.215 kb on - strand, within Pf1N1B4_257at 317.924 kb on - strand, within Pf1N1B4_257at 317.966 kb on + strand, within Pf1N1B4_257at 317.966 kb on + strand, within Pf1N1B4_257at 317.966 kb on + strand, within Pf1N1B4_257at 317.966 kb on + strand, within Pf1N1B4_257at 317.966 kb on + strand, within Pf1N1B4_257at 317.966 kb on + strand, within Pf1N1B4_257at 317.967 kb on - strandat 317.967 kb on - strand, within Pf1N1B4_257at 317.968 kb on + strand, within Pf1N1B4_257at 317.968 kb on + strand, within Pf1N1B4_257at 317.969 kb on - strand, within Pf1N1B4_257at 318.266 kb on - strandat 318.384 kb on - strand, within Pf1N1B4_257at 318.384 kb on - strand, within Pf1N1B4_257at 318.645 kb on - strandat 318.826 kb on + strandat 318.913 kb on - strand, within Pf1N1B4_258at 319.157 kb on - strand, within Pf1N1B4_258at 319.191 kb on - strand, within Pf1N1B4_258at 319.191 kb on - strand, within Pf1N1B4_258at 319.191 kb on - strand, within Pf1N1B4_258at 319.191 kb on - strand, within Pf1N1B4_258at 319.191 kb on - strand, within Pf1N1B4_258at 319.191 kb on - strand, within Pf1N1B4_258at 319.191 kb on - strand, within Pf1N1B4_258at 319.191 kb on - strand, within Pf1N1B4_258at 319.191 kb on - strand, within Pf1N1B4_258at 319.191 kb on - strand, within Pf1N1B4_258at 319.199 kb on - strand, within Pf1N1B4_258at 319.199 kb on - strand, within Pf1N1B4_258at 319.199 kb on - strand, within Pf1N1B4_258at 319.199 kb on - strand, within Pf1N1B4_258at 319.229 kb on - strand, within Pf1N1B4_258at 319.229 kb on - strand, within Pf1N1B4_258at 319.229 kb on - strand, within Pf1N1B4_258at 319.229 kb on - strand, within Pf1N1B4_258at 319.229 kb on - strand, within Pf1N1B4_258at 319.280 kb on + strandat 319.281 kb on - strandat 319.281 kb on - strandat 319.281 kb on - strandat 319.281 kb on - strandat 319.281 kb on - strandat 319.281 kb on - strandat 319.281 kb on - strandat 319.509 kb on + strand, within Pf1N1B4_259at 319.611 kb on + strand, within Pf1N1B4_259at 319.611 kb on + strand, within Pf1N1B4_259at 319.611 kb on + strand, within Pf1N1B4_259at 319.612 kb on - strand, within Pf1N1B4_259at 319.641 kb on + strand, within Pf1N1B4_259at 319.664 kb on - strand, within Pf1N1B4_259

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1
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315,529 - -1.3
315,685 - Pf1N1B4_256 0.23 +1.0
315,685 - Pf1N1B4_256 0.23 -0.0
315,685 - Pf1N1B4_256 0.23 -1.5
315,832 - Pf1N1B4_256 0.40 -0.0
315,832 - Pf1N1B4_256 0.40 -1.2
315,832 - Pf1N1B4_256 0.40 -0.0
315,844 + Pf1N1B4_256 0.41 -1.9
315,844 + Pf1N1B4_256 0.41 -0.0
316,392 + -0.5
316,392 + -0.0
316,392 + -0.8
316,393 - -0.0
316,393 - -3.3
316,393 - -0.0
316,393 - -3.6
316,393 - -0.0
316,393 - -2.9
316,427 - -0.0
316,434 + -0.0
316,434 + -0.0
316,435 - -3.5
316,435 - -3.4
316,477 + -0.0
316,477 + +0.7
316,477 + -0.4
316,477 + -0.0
316,478 - -0.0
316,551 - -0.0
316,551 - -1.3
316,551 - -0.0
316,551 - -3.2
316,618 - -3.5
316,618 - -0.0
316,628 + -0.0
316,629 - -0.0
316,662 + -0.0
316,663 - -3.8
316,663 - -0.0
316,901 + Pf1N1B4_257 0.18 -3.4
316,958 + Pf1N1B4_257 0.20 -1.9
316,958 + Pf1N1B4_257 0.20 -0.0
316,959 - -0.0
316,959 - Pf1N1B4_257 0.20 -3.5
316,959 - Pf1N1B4_257 0.20 -0.0
316,959 - Pf1N1B4_257 0.20 -4.0
316,959 - Pf1N1B4_257 0.20 -0.0
316,959 - Pf1N1B4_257 0.20 -0.0
316,959 - Pf1N1B4_257 0.20 -0.0
316,959 - Pf1N1B4_257 0.20 -0.0
316,959 - Pf1N1B4_257 0.20 -0.0
317,057 + Pf1N1B4_257 0.24 -0.0
317,058 - -4.0
317,058 - Pf1N1B4_257 0.25 -0.0
317,214 + Pf1N1B4_257 0.31 -0.0
317,215 - Pf1N1B4_257 0.31 -1.0
317,215 - Pf1N1B4_257 0.31 -0.0
317,924 - Pf1N1B4_257 0.61 -2.9
317,966 + Pf1N1B4_257 0.63 -0.0
317,966 + Pf1N1B4_257 0.63 -0.0
317,966 + Pf1N1B4_257 0.63 -0.0
317,966 + Pf1N1B4_257 0.63 -0.0
317,966 + Pf1N1B4_257 0.63 -3.9
317,966 + Pf1N1B4_257 0.63 -0.0
317,967 - -5.4
317,967 - Pf1N1B4_257 0.63 -0.0
317,968 + Pf1N1B4_257 0.63 -0.0
317,968 + Pf1N1B4_257 0.63 -0.0
317,969 - Pf1N1B4_257 0.63 -0.0
318,266 - -0.0
318,384 - Pf1N1B4_257 0.81 -0.0
318,384 - Pf1N1B4_257 0.81 -0.0
318,645 - -0.0
318,826 + -0.0
318,913 - Pf1N1B4_258 0.19 -0.0
319,157 - Pf1N1B4_258 0.72 -0.0
319,191 - Pf1N1B4_258 0.79 -0.0
319,191 - Pf1N1B4_258 0.79 -0.0
319,191 - Pf1N1B4_258 0.79 -0.0
319,191 - Pf1N1B4_258 0.79 -2.9
319,191 - Pf1N1B4_258 0.79 -0.9
319,191 - Pf1N1B4_258 0.79 -0.0
319,191 - Pf1N1B4_258 0.79 -0.0
319,191 - Pf1N1B4_258 0.79 -0.0
319,191 - Pf1N1B4_258 0.79 -0.0
319,191 - Pf1N1B4_258 0.79 -0.0
319,199 - Pf1N1B4_258 0.81 -0.3
319,199 - Pf1N1B4_258 0.81 -0.0
319,199 - Pf1N1B4_258 0.81 -0.0
319,199 - Pf1N1B4_258 0.81 -2.8
319,229 - Pf1N1B4_258 0.87 -0.0
319,229 - Pf1N1B4_258 0.87 -2.5
319,229 - Pf1N1B4_258 0.87 -0.0
319,229 - Pf1N1B4_258 0.87 -0.0
319,229 - Pf1N1B4_258 0.87 -1.4
319,280 + -0.9
319,281 - -0.0
319,281 - -0.0
319,281 - -2.0
319,281 - -2.0
319,281 - -1.5
319,281 - -0.0
319,281 - -0.0
319,509 + Pf1N1B4_259 0.47 -0.0
319,611 + Pf1N1B4_259 0.67 -1.6
319,611 + Pf1N1B4_259 0.67 -0.0
319,611 + Pf1N1B4_259 0.67 -0.0
319,612 - Pf1N1B4_259 0.68 -0.0
319,641 + Pf1N1B4_259 0.74 -0.0
319,664 - Pf1N1B4_259 0.78 -1.8

Or see this region's nucleotide sequence