Experiment: Nitrate reduction, replicate A t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2336 and Pf1N1B4_2337 are separated by 26 nucleotides Pf1N1B4_2337 and Pf1N1B4_2338 are separated by 7 nucleotides Pf1N1B4_2338 and Pf1N1B4_2339 are separated by 183 nucleotides Pf1N1B4_2339 and Pf1N1B4_2340 overlap by 1 nucleotides
Pf1N1B4_2336 - FIG003551: hypothetical protein, at 2,653,641 to 2,654,075
_2336
Pf1N1B4_2337 - Homoserine O-acetyltransferase (EC 2.3.1.31), at 2,654,102 to 2,654,722
_2337
Pf1N1B4_2338 - Homoserine O-acetyltransferase (EC 2.3.1.31), at 2,654,730 to 2,655,869
_2338
Pf1N1B4_2339 - COG1872, at 2,656,053 to 2,656,349
_2339
Pf1N1B4_2340 - Integral membrane protein YggT, involved in response to extracytoplasmic stress (osmotic shock), at 2,656,349 to 2,656,939
_2340
Position (kb)
2654
2655
2656 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2653.756 kb on + strand, within Pf1N1B4_2336 at 2653.756 kb on + strand, within Pf1N1B4_2336 at 2653.756 kb on + strand, within Pf1N1B4_2336 at 2653.756 kb on + strand at 2653.756 kb on + strand, within Pf1N1B4_2336 at 2653.756 kb on + strand, within Pf1N1B4_2336 at 2653.756 kb on + strand, within Pf1N1B4_2336 at 2653.757 kb on - strand, within Pf1N1B4_2336 at 2653.757 kb on - strand, within Pf1N1B4_2336 at 2653.759 kb on + strand, within Pf1N1B4_2336 at 2653.856 kb on + strand at 2653.857 kb on - strand, within Pf1N1B4_2336 at 2653.963 kb on + strand, within Pf1N1B4_2336 at 2653.963 kb on + strand, within Pf1N1B4_2336 at 2653.963 kb on + strand, within Pf1N1B4_2336 at 2653.963 kb on + strand, within Pf1N1B4_2336 at 2653.963 kb on + strand, within Pf1N1B4_2336 at 2653.963 kb on + strand, within Pf1N1B4_2336 at 2653.964 kb on - strand, within Pf1N1B4_2336 at 2653.964 kb on - strand, within Pf1N1B4_2336 at 2654.048 kb on + strand at 2654.049 kb on - strand at 2654.049 kb on - strand at 2654.052 kb on + strand at 2654.052 kb on + strand at 2654.053 kb on - strand at 2654.064 kb on + strand at 2654.064 kb on + strand at 2654.165 kb on + strand, within Pf1N1B4_2337 at 2654.174 kb on - strand, within Pf1N1B4_2337 at 2654.180 kb on + strand, within Pf1N1B4_2337 at 2654.180 kb on + strand, within Pf1N1B4_2337 at 2654.180 kb on + strand, within Pf1N1B4_2337 at 2654.180 kb on + strand, within Pf1N1B4_2337 at 2654.181 kb on - strand, within Pf1N1B4_2337 at 2654.181 kb on - strand, within Pf1N1B4_2337 at 2654.307 kb on + strand, within Pf1N1B4_2337 at 2654.307 kb on + strand, within Pf1N1B4_2337 at 2654.307 kb on + strand, within Pf1N1B4_2337 at 2654.308 kb on - strand, within Pf1N1B4_2337 at 2654.316 kb on + strand, within Pf1N1B4_2337 at 2654.316 kb on + strand, within Pf1N1B4_2337 at 2654.316 kb on + strand, within Pf1N1B4_2337 at 2654.316 kb on + strand, within Pf1N1B4_2337 at 2654.316 kb on + strand, within Pf1N1B4_2337 at 2654.316 kb on + strand, within Pf1N1B4_2337 at 2654.316 kb on + strand at 2654.317 kb on - strand, within Pf1N1B4_2337 at 2654.317 kb on - strand, within Pf1N1B4_2337 at 2654.451 kb on + strand, within Pf1N1B4_2337 at 2654.739 kb on + strand at 2654.739 kb on + strand at 2654.741 kb on + strand at 2654.741 kb on + strand at 2654.742 kb on - strand at 2654.752 kb on + strand at 2654.752 kb on + strand at 2654.752 kb on + strand at 2654.752 kb on + strand at 2654.752 kb on + strand at 2654.752 kb on + strand at 2654.752 kb on + strand at 2654.752 kb on + strand at 2654.752 kb on + strand at 2654.753 kb on - strand at 2654.753 kb on - strand at 2654.773 kb on + strand at 2654.773 kb on + strand at 2654.773 kb on + strand at 2654.773 kb on + strand at 2654.993 kb on - strand, within Pf1N1B4_2338 at 2654.993 kb on - strand, within Pf1N1B4_2338 at 2654.993 kb on - strand, within Pf1N1B4_2338 at 2655.019 kb on + strand, within Pf1N1B4_2338 at 2655.019 kb on + strand, within Pf1N1B4_2338 at 2655.019 kb on + strand, within Pf1N1B4_2338 at 2655.019 kb on + strand, within Pf1N1B4_2338 at 2655.019 kb on + strand, within Pf1N1B4_2338 at 2655.020 kb on - strand, within Pf1N1B4_2338 at 2655.020 kb on - strand, within Pf1N1B4_2338 at 2655.062 kb on - strand, within Pf1N1B4_2338 at 2655.070 kb on + strand, within Pf1N1B4_2338 at 2655.070 kb on + strand, within Pf1N1B4_2338 at 2655.070 kb on + strand, within Pf1N1B4_2338 at 2655.070 kb on + strand, within Pf1N1B4_2338 at 2655.071 kb on - strand, within Pf1N1B4_2338 at 2655.071 kb on - strand, within Pf1N1B4_2338 at 2655.106 kb on + strand, within Pf1N1B4_2338 at 2655.106 kb on + strand, within Pf1N1B4_2338 at 2655.107 kb on - strand, within Pf1N1B4_2338 at 2655.107 kb on - strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.170 kb on - strand, within Pf1N1B4_2338 at 2655.170 kb on - strand, within Pf1N1B4_2338 at 2655.170 kb on - strand, within Pf1N1B4_2338 at 2655.170 kb on - strand, within Pf1N1B4_2338 at 2655.569 kb on + strand, within Pf1N1B4_2338 at 2655.569 kb on + strand at 2655.569 kb on + strand, within Pf1N1B4_2338 at 2655.570 kb on - strand, within Pf1N1B4_2338 at 2655.745 kb on + strand, within Pf1N1B4_2338 at 2655.745 kb on + strand, within Pf1N1B4_2338 at 2655.746 kb on - strand, within Pf1N1B4_2338 at 2655.754 kb on + strand, within Pf1N1B4_2338 at 2655.754 kb on + strand, within Pf1N1B4_2338 at 2655.754 kb on + strand, within Pf1N1B4_2338 at 2655.766 kb on + strand at 2655.766 kb on + strand at 2655.766 kb on + strand at 2655.766 kb on + strand at 2655.766 kb on + strand at 2655.767 kb on - strand at 2655.767 kb on - strand at 2655.897 kb on + strand at 2655.897 kb on + strand at 2655.897 kb on + strand at 2655.897 kb on + strand at 2655.898 kb on - strand at 2655.898 kb on - strand at 2655.903 kb on + strand at 2655.903 kb on + strand at 2656.201 kb on + strand, within Pf1N1B4_2339 at 2656.202 kb on - strand, within Pf1N1B4_2339 at 2656.202 kb on - strand, within Pf1N1B4_2339 at 2656.202 kb on - strand, within Pf1N1B4_2339 at 2656.342 kb on + strand at 2656.342 kb on + strand at 2656.342 kb on + strand at 2656.343 kb on - strand at 2656.343 kb on - strand at 2656.343 kb on - strand at 2656.343 kb on - strand at 2656.386 kb on + strand at 2656.386 kb on + strand at 2656.386 kb on + strand at 2656.387 kb on - strand at 2656.387 kb on - strand at 2656.387 kb on - strand at 2656.387 kb on - strand at 2656.387 kb on - strand at 2656.387 kb on - strand at 2656.389 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1 remove 2,653,756 + Pf1N1B4_2336 0.26 -1.8 2,653,756 + Pf1N1B4_2336 0.26 -0.0 2,653,756 + Pf1N1B4_2336 0.26 -0.0 2,653,756 + -0.3 2,653,756 + Pf1N1B4_2336 0.26 -0.0 2,653,756 + Pf1N1B4_2336 0.26 -0.0 2,653,756 + Pf1N1B4_2336 0.26 -0.0 2,653,757 - Pf1N1B4_2336 0.27 -0.0 2,653,757 - Pf1N1B4_2336 0.27 -0.0 2,653,759 + Pf1N1B4_2336 0.27 -1.8 2,653,856 + -0.8 2,653,857 - Pf1N1B4_2336 0.50 -0.0 2,653,963 + Pf1N1B4_2336 0.74 -0.0 2,653,963 + Pf1N1B4_2336 0.74 -2.0 2,653,963 + Pf1N1B4_2336 0.74 -2.5 2,653,963 + Pf1N1B4_2336 0.74 -0.0 2,653,963 + Pf1N1B4_2336 0.74 -0.0 2,653,963 + Pf1N1B4_2336 0.74 -0.0 2,653,964 - Pf1N1B4_2336 0.74 -1.5 2,653,964 - Pf1N1B4_2336 0.74 -1.7 2,654,048 + -0.0 2,654,049 - -2.1 2,654,049 - -1.9 2,654,052 + -1.5 2,654,052 + -0.0 2,654,053 - -0.0 2,654,064 + -0.0 2,654,064 + -0.0 2,654,165 + Pf1N1B4_2337 0.10 +0.6 2,654,174 - Pf1N1B4_2337 0.12 -0.0 2,654,180 + Pf1N1B4_2337 0.13 -2.4 2,654,180 + Pf1N1B4_2337 0.13 -3.6 2,654,180 + Pf1N1B4_2337 0.13 -1.6 2,654,180 + Pf1N1B4_2337 0.13 -0.0 2,654,181 - Pf1N1B4_2337 0.13 -3.2 2,654,181 - Pf1N1B4_2337 0.13 -0.0 2,654,307 + Pf1N1B4_2337 0.33 -2.3 2,654,307 + Pf1N1B4_2337 0.33 -0.0 2,654,307 + Pf1N1B4_2337 0.33 -0.0 2,654,308 - Pf1N1B4_2337 0.33 -0.0 2,654,316 + Pf1N1B4_2337 0.34 -0.0 2,654,316 + Pf1N1B4_2337 0.34 -0.0 2,654,316 + Pf1N1B4_2337 0.34 -0.0 2,654,316 + Pf1N1B4_2337 0.34 -0.0 2,654,316 + Pf1N1B4_2337 0.34 -0.0 2,654,316 + Pf1N1B4_2337 0.34 -0.0 2,654,316 + -0.0 2,654,317 - Pf1N1B4_2337 0.35 -1.5 2,654,317 - Pf1N1B4_2337 0.35 -3.8 2,654,451 + Pf1N1B4_2337 0.56 +1.0 2,654,739 + -0.0 2,654,739 + -0.0 2,654,741 + -0.0 2,654,741 + -3.4 2,654,742 - -0.0 2,654,752 + -5.1 2,654,752 + -1.0 2,654,752 + -0.0 2,654,752 + -0.0 2,654,752 + -4.6 2,654,752 + -3.6 2,654,752 + -0.0 2,654,752 + -3.8 2,654,752 + -3.8 2,654,753 - -0.0 2,654,753 - -0.0 2,654,773 + -0.0 2,654,773 + -3.8 2,654,773 + -0.0 2,654,773 + -0.0 2,654,993 - Pf1N1B4_2338 0.23 -3.3 2,654,993 - Pf1N1B4_2338 0.23 -0.0 2,654,993 - Pf1N1B4_2338 0.23 -0.0 2,655,019 + Pf1N1B4_2338 0.25 -4.6 2,655,019 + Pf1N1B4_2338 0.25 -0.0 2,655,019 + Pf1N1B4_2338 0.25 -0.0 2,655,019 + Pf1N1B4_2338 0.25 -0.0 2,655,019 + Pf1N1B4_2338 0.25 -1.4 2,655,020 - Pf1N1B4_2338 0.25 -0.0 2,655,020 - Pf1N1B4_2338 0.25 -4.4 2,655,062 - Pf1N1B4_2338 0.29 -0.0 2,655,070 + Pf1N1B4_2338 0.30 -0.0 2,655,070 + Pf1N1B4_2338 0.30 -0.0 2,655,070 + Pf1N1B4_2338 0.30 -0.0 2,655,070 + Pf1N1B4_2338 0.30 -0.0 2,655,071 - Pf1N1B4_2338 0.30 -0.0 2,655,071 - Pf1N1B4_2338 0.30 -0.0 2,655,106 + Pf1N1B4_2338 0.33 -0.0 2,655,106 + Pf1N1B4_2338 0.33 -3.7 2,655,107 - Pf1N1B4_2338 0.33 -3.1 2,655,107 - Pf1N1B4_2338 0.33 -0.0 2,655,169 + Pf1N1B4_2338 0.39 -0.0 2,655,169 + Pf1N1B4_2338 0.39 -0.0 2,655,169 + Pf1N1B4_2338 0.39 -0.0 2,655,169 + Pf1N1B4_2338 0.39 -0.0 2,655,169 + Pf1N1B4_2338 0.39 -0.0 2,655,169 + Pf1N1B4_2338 0.39 -4.4 2,655,169 + Pf1N1B4_2338 0.39 -0.0 2,655,169 + Pf1N1B4_2338 0.39 -0.0 2,655,169 + Pf1N1B4_2338 0.39 -0.0 2,655,170 - Pf1N1B4_2338 0.39 -1.6 2,655,170 - Pf1N1B4_2338 0.39 -3.8 2,655,170 - Pf1N1B4_2338 0.39 -0.0 2,655,170 - Pf1N1B4_2338 0.39 -0.0 2,655,569 + Pf1N1B4_2338 0.74 -0.0 2,655,569 + -0.0 2,655,569 + Pf1N1B4_2338 0.74 -1.0 2,655,570 - Pf1N1B4_2338 0.74 -5.4 2,655,745 + Pf1N1B4_2338 0.89 -0.0 2,655,745 + Pf1N1B4_2338 0.89 -0.0 2,655,746 - Pf1N1B4_2338 0.89 -0.0 2,655,754 + Pf1N1B4_2338 0.90 -0.0 2,655,754 + Pf1N1B4_2338 0.90 -0.0 2,655,754 + Pf1N1B4_2338 0.90 -0.0 2,655,766 + -0.0 2,655,766 + -0.0 2,655,766 + -0.0 2,655,766 + -0.0 2,655,766 + -0.0 2,655,767 - -0.0 2,655,767 - -0.0 2,655,897 + -0.0 2,655,897 + -3.3 2,655,897 + -0.0 2,655,897 + -0.0 2,655,898 - -1.0 2,655,898 - -2.0 2,655,903 + -0.0 2,655,903 + -0.0 2,656,201 + Pf1N1B4_2339 0.50 -0.0 2,656,202 - Pf1N1B4_2339 0.50 -0.0 2,656,202 - Pf1N1B4_2339 0.50 -0.7 2,656,202 - Pf1N1B4_2339 0.50 -0.0 2,656,342 + -0.0 2,656,342 + -0.0 2,656,342 + -1.0 2,656,343 - -0.0 2,656,343 - -0.9 2,656,343 - -1.2 2,656,343 - -0.0 2,656,386 + -0.0 2,656,386 + -1.6 2,656,386 + -0.4 2,656,387 - -0.0 2,656,387 - -0.0 2,656,387 - -0.0 2,656,387 - -0.0 2,656,387 - -0.0 2,656,387 - -0.0 2,656,389 + -1.8
Or see this region's nucleotide sequence