Experiment: Nitrate reduction, replicate A t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2334 and Pf1N1B4_2335 overlap by 4 nucleotides Pf1N1B4_2335 and Pf1N1B4_2336 overlap by 4 nucleotides Pf1N1B4_2336 and Pf1N1B4_2337 are separated by 26 nucleotides
Pf1N1B4_2334 - Radical SAM family enzyme, similar to coproporphyrinogen III oxidase, oxygen-independent, clustered with nucleoside-triphosphatase RdgB, at 2,651,846 to 2,653,051
_2334
Pf1N1B4_2335 - Nucleoside 5-triphosphatase RdgB (dHAPTP, dITP, XTP-specific) (EC 3.6.1.15), at 2,653,048 to 2,653,644
_2335
Pf1N1B4_2336 - FIG003551: hypothetical protein, at 2,653,641 to 2,654,075
_2336
Pf1N1B4_2337 - Homoserine O-acetyltransferase (EC 2.3.1.31), at 2,654,102 to 2,654,722
_2337
Position (kb)
2653
2654 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2652.117 kb on + strand, within Pf1N1B4_2334 at 2652.118 kb on - strand, within Pf1N1B4_2334 at 2652.235 kb on + strand, within Pf1N1B4_2334 at 2652.279 kb on + strand at 2652.502 kb on + strand, within Pf1N1B4_2334 at 2652.822 kb on + strand, within Pf1N1B4_2334 at 2652.870 kb on + strand, within Pf1N1B4_2334 at 2652.870 kb on + strand, within Pf1N1B4_2334 at 2652.871 kb on - strand, within Pf1N1B4_2334 at 2652.921 kb on + strand, within Pf1N1B4_2334 at 2652.922 kb on - strand, within Pf1N1B4_2334 at 2652.957 kb on + strand at 2652.957 kb on + strand at 2652.957 kb on + strand at 2652.958 kb on - strand at 2652.958 kb on - strand at 2652.958 kb on - strand at 2653.125 kb on + strand, within Pf1N1B4_2335 at 2653.125 kb on + strand, within Pf1N1B4_2335 at 2653.126 kb on - strand, within Pf1N1B4_2335 at 2653.126 kb on - strand, within Pf1N1B4_2335 at 2653.126 kb on - strand, within Pf1N1B4_2335 at 2653.126 kb on - strand, within Pf1N1B4_2335 at 2653.389 kb on - strand, within Pf1N1B4_2335 at 2653.389 kb on - strand, within Pf1N1B4_2335 at 2653.389 kb on - strand, within Pf1N1B4_2335 at 2653.756 kb on + strand, within Pf1N1B4_2336 at 2653.756 kb on + strand, within Pf1N1B4_2336 at 2653.756 kb on + strand, within Pf1N1B4_2336 at 2653.756 kb on + strand at 2653.756 kb on + strand, within Pf1N1B4_2336 at 2653.756 kb on + strand, within Pf1N1B4_2336 at 2653.756 kb on + strand, within Pf1N1B4_2336 at 2653.757 kb on - strand, within Pf1N1B4_2336 at 2653.757 kb on - strand, within Pf1N1B4_2336 at 2653.759 kb on + strand, within Pf1N1B4_2336 at 2653.856 kb on + strand at 2653.857 kb on - strand, within Pf1N1B4_2336 at 2653.963 kb on + strand, within Pf1N1B4_2336 at 2653.963 kb on + strand, within Pf1N1B4_2336 at 2653.963 kb on + strand, within Pf1N1B4_2336 at 2653.963 kb on + strand, within Pf1N1B4_2336 at 2653.963 kb on + strand, within Pf1N1B4_2336 at 2653.963 kb on + strand, within Pf1N1B4_2336 at 2653.964 kb on - strand, within Pf1N1B4_2336 at 2653.964 kb on - strand, within Pf1N1B4_2336 at 2654.048 kb on + strand at 2654.049 kb on - strand at 2654.049 kb on - strand at 2654.052 kb on + strand at 2654.052 kb on + strand at 2654.053 kb on - strand at 2654.064 kb on + strand at 2654.064 kb on + strand at 2654.165 kb on + strand, within Pf1N1B4_2337 at 2654.174 kb on - strand, within Pf1N1B4_2337 at 2654.180 kb on + strand, within Pf1N1B4_2337 at 2654.180 kb on + strand, within Pf1N1B4_2337 at 2654.180 kb on + strand, within Pf1N1B4_2337 at 2654.180 kb on + strand, within Pf1N1B4_2337 at 2654.181 kb on - strand, within Pf1N1B4_2337 at 2654.181 kb on - strand, within Pf1N1B4_2337 at 2654.307 kb on + strand, within Pf1N1B4_2337 at 2654.307 kb on + strand, within Pf1N1B4_2337 at 2654.307 kb on + strand, within Pf1N1B4_2337 at 2654.308 kb on - strand, within Pf1N1B4_2337 at 2654.316 kb on + strand, within Pf1N1B4_2337 at 2654.316 kb on + strand, within Pf1N1B4_2337 at 2654.316 kb on + strand, within Pf1N1B4_2337 at 2654.316 kb on + strand, within Pf1N1B4_2337 at 2654.316 kb on + strand, within Pf1N1B4_2337 at 2654.316 kb on + strand, within Pf1N1B4_2337 at 2654.316 kb on + strand at 2654.317 kb on - strand, within Pf1N1B4_2337 at 2654.317 kb on - strand, within Pf1N1B4_2337 at 2654.451 kb on + strand, within Pf1N1B4_2337
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1 remove 2,652,117 + Pf1N1B4_2334 0.22 -0.0 2,652,118 - Pf1N1B4_2334 0.23 -0.0 2,652,235 + Pf1N1B4_2334 0.32 -0.0 2,652,279 + -0.0 2,652,502 + Pf1N1B4_2334 0.54 -2.2 2,652,822 + Pf1N1B4_2334 0.81 -0.0 2,652,870 + Pf1N1B4_2334 0.85 -2.8 2,652,870 + Pf1N1B4_2334 0.85 -0.0 2,652,871 - Pf1N1B4_2334 0.85 -0.0 2,652,921 + Pf1N1B4_2334 0.89 -2.5 2,652,922 - Pf1N1B4_2334 0.89 -0.0 2,652,957 + -0.0 2,652,957 + -0.0 2,652,957 + -0.0 2,652,958 - -1.8 2,652,958 - -2.0 2,652,958 - -0.0 2,653,125 + Pf1N1B4_2335 0.13 -0.0 2,653,125 + Pf1N1B4_2335 0.13 -2.9 2,653,126 - Pf1N1B4_2335 0.13 -0.0 2,653,126 - Pf1N1B4_2335 0.13 -0.0 2,653,126 - Pf1N1B4_2335 0.13 -0.0 2,653,126 - Pf1N1B4_2335 0.13 -0.0 2,653,389 - Pf1N1B4_2335 0.57 -2.3 2,653,389 - Pf1N1B4_2335 0.57 -3.0 2,653,389 - Pf1N1B4_2335 0.57 -0.0 2,653,756 + Pf1N1B4_2336 0.26 -1.8 2,653,756 + Pf1N1B4_2336 0.26 -0.0 2,653,756 + Pf1N1B4_2336 0.26 -0.0 2,653,756 + -0.3 2,653,756 + Pf1N1B4_2336 0.26 -0.0 2,653,756 + Pf1N1B4_2336 0.26 -0.0 2,653,756 + Pf1N1B4_2336 0.26 -0.0 2,653,757 - Pf1N1B4_2336 0.27 -0.0 2,653,757 - Pf1N1B4_2336 0.27 -0.0 2,653,759 + Pf1N1B4_2336 0.27 -1.8 2,653,856 + -0.8 2,653,857 - Pf1N1B4_2336 0.50 -0.0 2,653,963 + Pf1N1B4_2336 0.74 -0.0 2,653,963 + Pf1N1B4_2336 0.74 -2.0 2,653,963 + Pf1N1B4_2336 0.74 -2.5 2,653,963 + Pf1N1B4_2336 0.74 -0.0 2,653,963 + Pf1N1B4_2336 0.74 -0.0 2,653,963 + Pf1N1B4_2336 0.74 -0.0 2,653,964 - Pf1N1B4_2336 0.74 -1.5 2,653,964 - Pf1N1B4_2336 0.74 -1.7 2,654,048 + -0.0 2,654,049 - -2.1 2,654,049 - -1.9 2,654,052 + -1.5 2,654,052 + -0.0 2,654,053 - -0.0 2,654,064 + -0.0 2,654,064 + -0.0 2,654,165 + Pf1N1B4_2337 0.10 +0.6 2,654,174 - Pf1N1B4_2337 0.12 -0.0 2,654,180 + Pf1N1B4_2337 0.13 -2.4 2,654,180 + Pf1N1B4_2337 0.13 -3.6 2,654,180 + Pf1N1B4_2337 0.13 -1.6 2,654,180 + Pf1N1B4_2337 0.13 -0.0 2,654,181 - Pf1N1B4_2337 0.13 -3.2 2,654,181 - Pf1N1B4_2337 0.13 -0.0 2,654,307 + Pf1N1B4_2337 0.33 -2.3 2,654,307 + Pf1N1B4_2337 0.33 -0.0 2,654,307 + Pf1N1B4_2337 0.33 -0.0 2,654,308 - Pf1N1B4_2337 0.33 -0.0 2,654,316 + Pf1N1B4_2337 0.34 -0.0 2,654,316 + Pf1N1B4_2337 0.34 -0.0 2,654,316 + Pf1N1B4_2337 0.34 -0.0 2,654,316 + Pf1N1B4_2337 0.34 -0.0 2,654,316 + Pf1N1B4_2337 0.34 -0.0 2,654,316 + Pf1N1B4_2337 0.34 -0.0 2,654,316 + -0.0 2,654,317 - Pf1N1B4_2337 0.35 -1.5 2,654,317 - Pf1N1B4_2337 0.35 -3.8 2,654,451 + Pf1N1B4_2337 0.56 +1.0
Or see this region's nucleotide sequence