Experiment: Nitrate reduction, replicate A t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2280 and Pf1N1B4_2281 are separated by 39 nucleotides Pf1N1B4_2281 and Pf1N1B4_2282 are separated by 54 nucleotides Pf1N1B4_2282 and Pf1N1B4_2283 are separated by 143 nucleotides
Pf1N1B4_2280 - Threonine dehydratase biosynthetic (EC 4.3.1.19), at 2,595,078 to 2,596,592
_2280
Pf1N1B4_2281 - Predicted integral membrane protein, at 2,596,632 to 2,597,123
_2281
Pf1N1B4_2282 - Phosphoserine phosphatase (EC 3.1.3.3), at 2,597,178 to 2,597,834
_2282
Pf1N1B4_2283 - Adenosine (5')-pentaphospho-(5'')-adenosine pyrophosphohydrolase (EC 3.6.1.-), at 2,597,978 to 2,598,457
_2283
Position (kb)
2596
2597
2598 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2595.651 kb on + strand, within Pf1N1B4_2280 at 2595.651 kb on + strand, within Pf1N1B4_2280 at 2595.651 kb on + strand, within Pf1N1B4_2280 at 2595.651 kb on + strand, within Pf1N1B4_2280 at 2595.652 kb on - strand, within Pf1N1B4_2280 at 2595.652 kb on - strand, within Pf1N1B4_2280 at 2595.819 kb on + strand, within Pf1N1B4_2280 at 2595.819 kb on + strand, within Pf1N1B4_2280 at 2595.819 kb on + strand, within Pf1N1B4_2280 at 2595.820 kb on - strand, within Pf1N1B4_2280 at 2595.820 kb on - strand, within Pf1N1B4_2280 at 2595.824 kb on - strand, within Pf1N1B4_2280 at 2595.876 kb on - strand, within Pf1N1B4_2280 at 2595.879 kb on + strand, within Pf1N1B4_2280 at 2595.880 kb on - strand, within Pf1N1B4_2280 at 2595.942 kb on + strand, within Pf1N1B4_2280 at 2595.942 kb on + strand, within Pf1N1B4_2280 at 2595.942 kb on + strand, within Pf1N1B4_2280 at 2595.942 kb on + strand, within Pf1N1B4_2280 at 2595.942 kb on + strand, within Pf1N1B4_2280 at 2595.942 kb on + strand, within Pf1N1B4_2280 at 2595.942 kb on + strand, within Pf1N1B4_2280 at 2595.942 kb on + strand, within Pf1N1B4_2280 at 2595.942 kb on + strand, within Pf1N1B4_2280 at 2595.942 kb on + strand, within Pf1N1B4_2280 at 2595.943 kb on - strand, within Pf1N1B4_2280 at 2595.943 kb on - strand, within Pf1N1B4_2280 at 2595.943 kb on - strand, within Pf1N1B4_2280 at 2595.943 kb on - strand at 2595.943 kb on - strand, within Pf1N1B4_2280 at 2596.152 kb on + strand, within Pf1N1B4_2280 at 2596.152 kb on + strand, within Pf1N1B4_2280 at 2596.159 kb on - strand, within Pf1N1B4_2280 at 2596.310 kb on + strand, within Pf1N1B4_2280 at 2596.310 kb on + strand, within Pf1N1B4_2280 at 2596.471 kb on + strand at 2596.471 kb on + strand at 2596.546 kb on - strand at 2596.546 kb on - strand at 2596.573 kb on - strand at 2596.573 kb on - strand at 2596.665 kb on - strand at 2596.665 kb on - strand at 2596.665 kb on - strand at 2596.760 kb on + strand, within Pf1N1B4_2281 at 2596.761 kb on - strand, within Pf1N1B4_2281 at 2596.761 kb on - strand, within Pf1N1B4_2281 at 2596.761 kb on - strand, within Pf1N1B4_2281 at 2596.761 kb on - strand, within Pf1N1B4_2281 at 2596.900 kb on - strand, within Pf1N1B4_2281 at 2596.900 kb on - strand, within Pf1N1B4_2281 at 2596.982 kb on + strand, within Pf1N1B4_2281 at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.125 kb on + strand at 2597.125 kb on + strand at 2597.126 kb on - strand at 2597.126 kb on - strand at 2597.126 kb on - strand at 2597.126 kb on - strand at 2597.126 kb on - strand at 2597.126 kb on - strand at 2597.126 kb on - strand at 2597.126 kb on - strand at 2597.305 kb on + strand, within Pf1N1B4_2282 at 2597.305 kb on + strand, within Pf1N1B4_2282 at 2597.305 kb on + strand, within Pf1N1B4_2282 at 2597.306 kb on - strand, within Pf1N1B4_2282 at 2597.306 kb on - strand, within Pf1N1B4_2282 at 2597.306 kb on - strand, within Pf1N1B4_2282 at 2597.306 kb on - strand, within Pf1N1B4_2282 at 2597.311 kb on + strand, within Pf1N1B4_2282 at 2597.311 kb on + strand, within Pf1N1B4_2282 at 2597.311 kb on + strand, within Pf1N1B4_2282 at 2597.311 kb on + strand, within Pf1N1B4_2282 at 2597.311 kb on + strand, within Pf1N1B4_2282 at 2597.311 kb on + strand, within Pf1N1B4_2282 at 2597.311 kb on + strand, within Pf1N1B4_2282 at 2597.311 kb on + strand, within Pf1N1B4_2282 at 2597.312 kb on - strand, within Pf1N1B4_2282 at 2597.312 kb on - strand, within Pf1N1B4_2282 at 2597.312 kb on - strand, within Pf1N1B4_2282 at 2597.312 kb on - strand, within Pf1N1B4_2282 at 2597.312 kb on - strand, within Pf1N1B4_2282 at 2597.312 kb on - strand, within Pf1N1B4_2282 at 2597.312 kb on - strand, within Pf1N1B4_2282 at 2597.312 kb on - strand, within Pf1N1B4_2282 at 2597.312 kb on - strand, within Pf1N1B4_2282 at 2597.596 kb on + strand, within Pf1N1B4_2282 at 2597.596 kb on + strand, within Pf1N1B4_2282 at 2597.596 kb on + strand, within Pf1N1B4_2282 at 2597.596 kb on + strand, within Pf1N1B4_2282 at 2597.596 kb on + strand, within Pf1N1B4_2282 at 2597.596 kb on + strand, within Pf1N1B4_2282 at 2597.597 kb on - strand, within Pf1N1B4_2282 at 2597.597 kb on - strand, within Pf1N1B4_2282 at 2597.665 kb on + strand, within Pf1N1B4_2282 at 2597.665 kb on + strand, within Pf1N1B4_2282 at 2597.695 kb on + strand, within Pf1N1B4_2282 at 2597.695 kb on + strand, within Pf1N1B4_2282 at 2597.695 kb on + strand, within Pf1N1B4_2282 at 2597.695 kb on + strand, within Pf1N1B4_2282 at 2597.696 kb on - strand, within Pf1N1B4_2282 at 2597.696 kb on - strand, within Pf1N1B4_2282 at 2597.704 kb on + strand, within Pf1N1B4_2282 at 2597.704 kb on + strand, within Pf1N1B4_2282 at 2597.704 kb on + strand, within Pf1N1B4_2282 at 2597.705 kb on - strand, within Pf1N1B4_2282 at 2597.728 kb on + strand, within Pf1N1B4_2282 at 2597.728 kb on + strand, within Pf1N1B4_2282 at 2597.728 kb on + strand, within Pf1N1B4_2282 at 2597.728 kb on + strand, within Pf1N1B4_2282 at 2597.728 kb on + strand, within Pf1N1B4_2282 at 2597.729 kb on - strand, within Pf1N1B4_2282 at 2597.764 kb on + strand, within Pf1N1B4_2282 at 2597.764 kb on + strand, within Pf1N1B4_2282 at 2597.764 kb on + strand, within Pf1N1B4_2282 at 2597.765 kb on - strand, within Pf1N1B4_2282 at 2597.765 kb on - strand, within Pf1N1B4_2282 at 2597.820 kb on + strand at 2597.835 kb on + strand at 2597.835 kb on + strand at 2597.835 kb on + strand at 2597.841 kb on + strand at 2597.842 kb on - strand at 2597.901 kb on + strand at 2597.901 kb on + strand at 2597.901 kb on + strand at 2597.901 kb on + strand at 2597.901 kb on + strand at 2597.901 kb on + strand at 2597.901 kb on + strand at 2597.901 kb on + strand at 2597.901 kb on + strand at 2597.901 kb on + strand at 2597.901 kb on + strand at 2597.901 kb on + strand at 2597.901 kb on + strand at 2597.901 kb on + strand at 2597.901 kb on + strand at 2597.901 kb on + strand at 2597.901 kb on + strand at 2597.901 kb on + strand at 2597.901 kb on + strand at 2597.901 kb on + strand at 2597.901 kb on + strand at 2597.901 kb on + strand at 2597.902 kb on - strand at 2597.902 kb on - strand at 2597.902 kb on - strand at 2597.902 kb on - strand at 2597.906 kb on + strand at 2597.906 kb on + strand at 2597.906 kb on + strand at 2597.906 kb on + strand at 2597.906 kb on + strand at 2597.906 kb on + strand at 2597.906 kb on + strand at 2597.906 kb on + strand at 2597.906 kb on + strand at 2597.906 kb on + strand at 2597.906 kb on + strand at 2597.906 kb on + strand at 2597.906 kb on + strand at 2597.906 kb on + strand at 2597.906 kb on + strand at 2597.906 kb on + strand at 2597.906 kb on + strand at 2597.906 kb on + strand at 2597.906 kb on + strand at 2597.906 kb on + strand at 2597.906 kb on + strand at 2597.906 kb on + strand at 2597.906 kb on + strand at 2597.906 kb on + strand at 2597.906 kb on + strand at 2597.906 kb on + strand at 2597.906 kb on + strand at 2597.906 kb on + strand at 2597.906 kb on + strand at 2597.907 kb on - strand at 2597.907 kb on - strand at 2597.907 kb on - strand at 2597.907 kb on - strand at 2597.907 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1 remove 2,595,651 + Pf1N1B4_2280 0.38 -3.6 2,595,651 + Pf1N1B4_2280 0.38 -0.0 2,595,651 + Pf1N1B4_2280 0.38 -0.0 2,595,651 + Pf1N1B4_2280 0.38 -0.0 2,595,652 - Pf1N1B4_2280 0.38 -1.6 2,595,652 - Pf1N1B4_2280 0.38 -0.0 2,595,819 + Pf1N1B4_2280 0.49 -5.0 2,595,819 + Pf1N1B4_2280 0.49 -3.7 2,595,819 + Pf1N1B4_2280 0.49 -0.0 2,595,820 - Pf1N1B4_2280 0.49 -0.0 2,595,820 - Pf1N1B4_2280 0.49 -3.8 2,595,824 - Pf1N1B4_2280 0.49 -0.0 2,595,876 - Pf1N1B4_2280 0.53 -3.4 2,595,879 + Pf1N1B4_2280 0.53 -3.9 2,595,880 - Pf1N1B4_2280 0.53 -0.0 2,595,942 + Pf1N1B4_2280 0.57 -0.0 2,595,942 + Pf1N1B4_2280 0.57 -0.0 2,595,942 + Pf1N1B4_2280 0.57 -0.0 2,595,942 + Pf1N1B4_2280 0.57 -0.0 2,595,942 + Pf1N1B4_2280 0.57 -3.5 2,595,942 + Pf1N1B4_2280 0.57 -0.0 2,595,942 + Pf1N1B4_2280 0.57 -0.0 2,595,942 + Pf1N1B4_2280 0.57 -3.6 2,595,942 + Pf1N1B4_2280 0.57 -2.8 2,595,942 + Pf1N1B4_2280 0.57 -3.8 2,595,943 - Pf1N1B4_2280 0.57 -0.0 2,595,943 - Pf1N1B4_2280 0.57 -0.0 2,595,943 - Pf1N1B4_2280 0.57 -0.0 2,595,943 - -0.0 2,595,943 - Pf1N1B4_2280 0.57 -0.0 2,596,152 + Pf1N1B4_2280 0.71 -0.0 2,596,152 + Pf1N1B4_2280 0.71 -3.6 2,596,159 - Pf1N1B4_2280 0.71 -0.0 2,596,310 + Pf1N1B4_2280 0.81 -0.0 2,596,310 + Pf1N1B4_2280 0.81 -0.0 2,596,471 + -0.0 2,596,471 + -2.9 2,596,546 - -0.0 2,596,546 - -0.0 2,596,573 - -3.6 2,596,573 - -2.9 2,596,665 - -0.0 2,596,665 - -2.9 2,596,665 - -0.0 2,596,760 + Pf1N1B4_2281 0.26 -0.0 2,596,761 - Pf1N1B4_2281 0.26 -0.0 2,596,761 - Pf1N1B4_2281 0.26 -2.7 2,596,761 - Pf1N1B4_2281 0.26 -2.0 2,596,761 - Pf1N1B4_2281 0.26 -0.0 2,596,900 - Pf1N1B4_2281 0.54 -0.0 2,596,900 - Pf1N1B4_2281 0.54 -2.9 2,596,982 + Pf1N1B4_2281 0.71 -0.0 2,597,121 + -3.9 2,597,121 + -0.0 2,597,121 + -0.0 2,597,121 + -0.0 2,597,121 + -0.0 2,597,121 + -0.0 2,597,121 + -3.6 2,597,121 + -0.0 2,597,121 + -0.0 2,597,121 + -0.0 2,597,121 + -0.0 2,597,121 + -1.4 2,597,121 + -0.0 2,597,121 + -0.0 2,597,121 + -3.0 2,597,121 + -0.0 2,597,121 + -0.0 2,597,121 + -0.0 2,597,121 + -0.0 2,597,121 + -0.5 2,597,121 + -1.5 2,597,121 + -0.7 2,597,121 + -0.0 2,597,121 + -0.0 2,597,121 + -0.0 2,597,121 + -1.4 2,597,121 + -0.0 2,597,122 - -0.0 2,597,122 - -0.0 2,597,122 - -1.6 2,597,122 - -0.0 2,597,122 - -0.0 2,597,122 - -0.0 2,597,122 - -3.0 2,597,122 - -0.0 2,597,122 - -1.5 2,597,122 - -0.0 2,597,122 - -0.0 2,597,122 - -1.0 2,597,122 - -1.0 2,597,122 - -0.0 2,597,122 - -0.0 2,597,122 - -0.0 2,597,122 - -0.0 2,597,122 - -0.0 2,597,122 - -0.0 2,597,122 - -0.0 2,597,122 - -0.0 2,597,122 - -0.0 2,597,122 - -0.0 2,597,122 - -0.0 2,597,122 - -0.0 2,597,122 - -0.0 2,597,125 + -1.5 2,597,125 + -0.0 2,597,126 - -0.0 2,597,126 - -2.4 2,597,126 - -1.1 2,597,126 - -0.0 2,597,126 - -0.0 2,597,126 - -0.0 2,597,126 - -0.0 2,597,126 - -1.4 2,597,305 + Pf1N1B4_2282 0.19 -0.0 2,597,305 + Pf1N1B4_2282 0.19 -2.5 2,597,305 + Pf1N1B4_2282 0.19 -0.0 2,597,306 - Pf1N1B4_2282 0.19 -0.0 2,597,306 - Pf1N1B4_2282 0.19 -0.0 2,597,306 - Pf1N1B4_2282 0.19 -1.7 2,597,306 - Pf1N1B4_2282 0.19 -5.2 2,597,311 + Pf1N1B4_2282 0.20 -0.0 2,597,311 + Pf1N1B4_2282 0.20 -0.0 2,597,311 + Pf1N1B4_2282 0.20 -0.0 2,597,311 + Pf1N1B4_2282 0.20 -0.0 2,597,311 + Pf1N1B4_2282 0.20 -3.4 2,597,311 + Pf1N1B4_2282 0.20 -0.0 2,597,311 + Pf1N1B4_2282 0.20 -4.4 2,597,311 + Pf1N1B4_2282 0.20 -0.0 2,597,312 - Pf1N1B4_2282 0.20 -1.9 2,597,312 - Pf1N1B4_2282 0.20 -0.0 2,597,312 - Pf1N1B4_2282 0.20 -0.0 2,597,312 - Pf1N1B4_2282 0.20 -0.0 2,597,312 - Pf1N1B4_2282 0.20 -0.0 2,597,312 - Pf1N1B4_2282 0.20 -1.3 2,597,312 - Pf1N1B4_2282 0.20 -2.0 2,597,312 - Pf1N1B4_2282 0.20 -2.6 2,597,312 - Pf1N1B4_2282 0.20 -3.0 2,597,596 + Pf1N1B4_2282 0.64 -0.0 2,597,596 + Pf1N1B4_2282 0.64 -0.0 2,597,596 + Pf1N1B4_2282 0.64 -0.0 2,597,596 + Pf1N1B4_2282 0.64 -0.0 2,597,596 + Pf1N1B4_2282 0.64 -2.6 2,597,596 + Pf1N1B4_2282 0.64 -0.0 2,597,597 - Pf1N1B4_2282 0.64 -0.0 2,597,597 - Pf1N1B4_2282 0.64 -2.1 2,597,665 + Pf1N1B4_2282 0.74 -0.0 2,597,665 + Pf1N1B4_2282 0.74 -2.9 2,597,695 + Pf1N1B4_2282 0.79 -3.3 2,597,695 + Pf1N1B4_2282 0.79 -0.0 2,597,695 + Pf1N1B4_2282 0.79 -0.0 2,597,695 + Pf1N1B4_2282 0.79 -0.0 2,597,696 - Pf1N1B4_2282 0.79 -0.0 2,597,696 - Pf1N1B4_2282 0.79 -1.9 2,597,704 + Pf1N1B4_2282 0.80 -2.8 2,597,704 + Pf1N1B4_2282 0.80 -0.0 2,597,704 + Pf1N1B4_2282 0.80 -0.0 2,597,705 - Pf1N1B4_2282 0.80 -0.0 2,597,728 + Pf1N1B4_2282 0.84 -0.0 2,597,728 + Pf1N1B4_2282 0.84 -3.0 2,597,728 + Pf1N1B4_2282 0.84 -2.2 2,597,728 + Pf1N1B4_2282 0.84 -0.0 2,597,728 + Pf1N1B4_2282 0.84 -3.1 2,597,729 - Pf1N1B4_2282 0.84 -0.0 2,597,764 + Pf1N1B4_2282 0.89 -5.5 2,597,764 + Pf1N1B4_2282 0.89 -0.0 2,597,764 + Pf1N1B4_2282 0.89 -0.0 2,597,765 - Pf1N1B4_2282 0.89 -0.0 2,597,765 - Pf1N1B4_2282 0.89 -0.0 2,597,820 + -3.2 2,597,835 + -0.0 2,597,835 + -0.0 2,597,835 + -0.0 2,597,841 + -1.6 2,597,842 - -0.4 2,597,901 + -0.0 2,597,901 + -0.0 2,597,901 + -2.9 2,597,901 + -2.0 2,597,901 + -0.0 2,597,901 + -0.0 2,597,901 + -2.5 2,597,901 + -0.0 2,597,901 + -1.8 2,597,901 + -0.0 2,597,901 + -0.0 2,597,901 + -4.0 2,597,901 + -0.0 2,597,901 + -0.0 2,597,901 + -0.0 2,597,901 + -4.4 2,597,901 + -0.0 2,597,901 + -0.0 2,597,901 + -0.0 2,597,901 + -0.0 2,597,901 + -0.0 2,597,901 + -5.2 2,597,902 - -0.0 2,597,902 - -0.0 2,597,902 - -0.0 2,597,902 - -0.0 2,597,906 + -1.2 2,597,906 + -2.4 2,597,906 + -1.9 2,597,906 + -0.0 2,597,906 + -0.0 2,597,906 + -0.0 2,597,906 + -3.6 2,597,906 + -0.0 2,597,906 + -0.0 2,597,906 + -0.0 2,597,906 + -2.6 2,597,906 + -0.0 2,597,906 + -0.0 2,597,906 + -2.8 2,597,906 + -2.1 2,597,906 + -0.0 2,597,906 + -0.0 2,597,906 + -3.7 2,597,906 + -0.0 2,597,906 + -0.0 2,597,906 + -0.0 2,597,906 + -2.6 2,597,906 + -2.5 2,597,906 + -0.0 2,597,906 + -3.0 2,597,906 + -2.3 2,597,906 + -0.0 2,597,906 + -2.8 2,597,906 + -0.0 2,597,907 - -0.4 2,597,907 - -1.3 2,597,907 - -0.0 2,597,907 - -0.0 2,597,907 - -0.0
Or see this region's nucleotide sequence