Experiment: Nitrate reduction, replicate A t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2272 and Pf1N1B4_2273 are separated by 27 nucleotides Pf1N1B4_2273 and Pf1N1B4_2274 are separated by 63 nucleotides
Pf1N1B4_2272 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 2,588,243 to 2,589,472
_2272
Pf1N1B4_2273 - hypothetical protein, at 2,589,500 to 2,589,613
_2273
Pf1N1B4_2274 - D-2-hydroxyglutarate dehydrogenase, at 2,589,677 to 2,591,071
_2274
Position (kb)
2589
2590 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2588.515 kb on + strand, within Pf1N1B4_2272 at 2588.530 kb on - strand, within Pf1N1B4_2272 at 2588.678 kb on + strand, within Pf1N1B4_2272 at 2588.678 kb on + strand, within Pf1N1B4_2272 at 2588.679 kb on - strand, within Pf1N1B4_2272 at 2588.679 kb on - strand, within Pf1N1B4_2272 at 2588.934 kb on + strand, within Pf1N1B4_2272 at 2588.935 kb on - strand, within Pf1N1B4_2272 at 2588.935 kb on - strand, within Pf1N1B4_2272 at 2588.935 kb on - strand, within Pf1N1B4_2272 at 2588.935 kb on - strand at 2588.940 kb on + strand, within Pf1N1B4_2272 at 2588.940 kb on + strand, within Pf1N1B4_2272 at 2588.941 kb on - strand, within Pf1N1B4_2272 at 2588.941 kb on - strand, within Pf1N1B4_2272 at 2588.941 kb on - strand, within Pf1N1B4_2272 at 2588.941 kb on - strand, within Pf1N1B4_2272 at 2588.941 kb on - strand, within Pf1N1B4_2272 at 2588.941 kb on - strand, within Pf1N1B4_2272 at 2589.363 kb on + strand at 2589.363 kb on + strand at 2589.363 kb on + strand at 2589.451 kb on + strand at 2589.479 kb on - strand at 2589.573 kb on + strand, within Pf1N1B4_2273 at 2589.574 kb on - strand, within Pf1N1B4_2273 at 2589.594 kb on + strand, within Pf1N1B4_2273 at 2589.599 kb on + strand, within Pf1N1B4_2273 at 2589.599 kb on + strand, within Pf1N1B4_2273 at 2589.599 kb on + strand, within Pf1N1B4_2273 at 2589.599 kb on + strand, within Pf1N1B4_2273 at 2589.599 kb on + strand, within Pf1N1B4_2273 at 2589.599 kb on + strand, within Pf1N1B4_2273 at 2589.600 kb on - strand, within Pf1N1B4_2273 at 2589.600 kb on - strand, within Pf1N1B4_2273 at 2589.600 kb on - strand, within Pf1N1B4_2273 at 2589.600 kb on - strand, within Pf1N1B4_2273 at 2589.600 kb on - strand, within Pf1N1B4_2273 at 2589.600 kb on - strand at 2589.600 kb on - strand, within Pf1N1B4_2273 at 2589.600 kb on - strand, within Pf1N1B4_2273 at 2589.600 kb on - strand, within Pf1N1B4_2273 at 2589.600 kb on - strand, within Pf1N1B4_2273 at 2589.600 kb on - strand, within Pf1N1B4_2273 at 2589.761 kb on + strand at 2589.762 kb on - strand at 2589.766 kb on + strand at 2589.953 kb on + strand, within Pf1N1B4_2274 at 2589.953 kb on + strand, within Pf1N1B4_2274 at 2589.953 kb on + strand, within Pf1N1B4_2274 at 2589.954 kb on - strand, within Pf1N1B4_2274 at 2589.954 kb on - strand, within Pf1N1B4_2274 at 2589.954 kb on - strand, within Pf1N1B4_2274 at 2589.954 kb on - strand, within Pf1N1B4_2274 at 2589.954 kb on - strand, within Pf1N1B4_2274 at 2590.059 kb on - strand, within Pf1N1B4_2274 at 2590.059 kb on - strand, within Pf1N1B4_2274 at 2590.216 kb on + strand, within Pf1N1B4_2274 at 2590.473 kb on + strand, within Pf1N1B4_2274 at 2590.473 kb on + strand, within Pf1N1B4_2274 at 2590.505 kb on + strand, within Pf1N1B4_2274
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1 remove 2,588,515 + Pf1N1B4_2272 0.22 -2.9 2,588,530 - Pf1N1B4_2272 0.23 -0.0 2,588,678 + Pf1N1B4_2272 0.35 -3.3 2,588,678 + Pf1N1B4_2272 0.35 -0.0 2,588,679 - Pf1N1B4_2272 0.35 -0.0 2,588,679 - Pf1N1B4_2272 0.35 -2.2 2,588,934 + Pf1N1B4_2272 0.56 -3.5 2,588,935 - Pf1N1B4_2272 0.56 -0.0 2,588,935 - Pf1N1B4_2272 0.56 -0.0 2,588,935 - Pf1N1B4_2272 0.56 -5.6 2,588,935 - -2.0 2,588,940 + Pf1N1B4_2272 0.57 -3.6 2,588,940 + Pf1N1B4_2272 0.57 -0.0 2,588,941 - Pf1N1B4_2272 0.57 -0.0 2,588,941 - Pf1N1B4_2272 0.57 -0.0 2,588,941 - Pf1N1B4_2272 0.57 -0.0 2,588,941 - Pf1N1B4_2272 0.57 -0.0 2,588,941 - Pf1N1B4_2272 0.57 -0.0 2,588,941 - Pf1N1B4_2272 0.57 -0.0 2,589,363 + -0.0 2,589,363 + -0.0 2,589,363 + -0.0 2,589,451 + -3.0 2,589,479 - -3.6 2,589,573 + Pf1N1B4_2273 0.64 -3.1 2,589,574 - Pf1N1B4_2273 0.65 -2.0 2,589,594 + Pf1N1B4_2273 0.82 -3.9 2,589,599 + Pf1N1B4_2273 0.87 -0.0 2,589,599 + Pf1N1B4_2273 0.87 -2.9 2,589,599 + Pf1N1B4_2273 0.87 -0.0 2,589,599 + Pf1N1B4_2273 0.87 -2.5 2,589,599 + Pf1N1B4_2273 0.87 -3.1 2,589,599 + Pf1N1B4_2273 0.87 -0.0 2,589,600 - Pf1N1B4_2273 0.88 -0.0 2,589,600 - Pf1N1B4_2273 0.88 -0.0 2,589,600 - Pf1N1B4_2273 0.88 -0.0 2,589,600 - Pf1N1B4_2273 0.88 -0.0 2,589,600 - Pf1N1B4_2273 0.88 -0.0 2,589,600 - -0.0 2,589,600 - Pf1N1B4_2273 0.88 -0.0 2,589,600 - Pf1N1B4_2273 0.88 -0.0 2,589,600 - Pf1N1B4_2273 0.88 -1.0 2,589,600 - Pf1N1B4_2273 0.88 -3.4 2,589,600 - Pf1N1B4_2273 0.88 -0.0 2,589,761 + -2.0 2,589,762 - -1.7 2,589,766 + -0.0 2,589,953 + Pf1N1B4_2274 0.20 -0.8 2,589,953 + Pf1N1B4_2274 0.20 -0.5 2,589,953 + Pf1N1B4_2274 0.20 -2.0 2,589,954 - Pf1N1B4_2274 0.20 -3.6 2,589,954 - Pf1N1B4_2274 0.20 -0.0 2,589,954 - Pf1N1B4_2274 0.20 -0.0 2,589,954 - Pf1N1B4_2274 0.20 -0.0 2,589,954 - Pf1N1B4_2274 0.20 -0.0 2,590,059 - Pf1N1B4_2274 0.27 -0.0 2,590,059 - Pf1N1B4_2274 0.27 -1.9 2,590,216 + Pf1N1B4_2274 0.39 -1.3 2,590,473 + Pf1N1B4_2274 0.57 -0.0 2,590,473 + Pf1N1B4_2274 0.57 -0.0 2,590,505 + Pf1N1B4_2274 0.59 -0.0
Or see this region's nucleotide sequence