Experiment: Nitrate reduction, replicate A t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_20 and Pf1N1B4_21 are separated by 48 nucleotides Pf1N1B4_21 and Pf1N1B4_22 are separated by 30 nucleotides Pf1N1B4_22 and Pf1N1B4_5160 are separated by 185 nucleotides Pf1N1B4_5160 and Pf1N1B4_5161 are separated by 82 nucleotides Pf1N1B4_5161 and Pf1N1B4_5162 are separated by 56 nucleotides Pf1N1B4_5162 and Pf1N1B4_5163 are separated by 82 nucleotides Pf1N1B4_5163 and Pf1N1B4_23 are separated by 190 nucleotides
Pf1N1B4_20 - FIG002571: 4-hydroxybenzoyl-CoA thioesterase domain protein, at 23,652 to 24,101
_20
Pf1N1B4_21 - Probable hydrolase, at 24,150 to 24,983
_21
Pf1N1B4_22 - Transcriptional regulator, TetR family, at 25,014 to 25,553
_22
Pf1N1B4_5160 - tRNA-Glu-TTC, at 25,739 to 25,811
_5160
Pf1N1B4_5161 - tRNA-Ala-GGC, at 25,894 to 25,966
_5161
Pf1N1B4_5162 - tRNA-Glu-TTC, at 26,023 to 26,095
_5162
Pf1N1B4_5163 - tRNA-Ala-GGC, at 26,178 to 26,250
_5163
Pf1N1B4_23 - Glutamyl-tRNA synthetase (EC 6.1.1.17), at 26,441 to 26,560
_23
Pf1N1B4_24 - Glutamyl-tRNA synthetase (EC 6.1.1.17), at 26,536 to 27,849
_24
Position (kb) 25
26 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 24.121 kb on + strand at 24.122 kb on - strand at 24.122 kb on - strand at 24.122 kb on - strand at 24.252 kb on + strand, within Pf1N1B4_21 at 24.253 kb on - strand, within Pf1N1B4_21 at 24.365 kb on - strand, within Pf1N1B4_21 at 24.423 kb on - strand, within Pf1N1B4_21 at 24.423 kb on - strand at 24.458 kb on - strand, within Pf1N1B4_21 at 24.458 kb on - strand, within Pf1N1B4_21 at 24.458 kb on - strand, within Pf1N1B4_21 at 24.458 kb on - strand, within Pf1N1B4_21 at 24.478 kb on - strand, within Pf1N1B4_21 at 24.775 kb on - strand, within Pf1N1B4_21 at 25.285 kb on + strand, within Pf1N1B4_22 at 25.286 kb on - strand, within Pf1N1B4_22 at 25.286 kb on - strand, within Pf1N1B4_22 at 25.368 kb on + strand, within Pf1N1B4_22 at 25.369 kb on - strand, within Pf1N1B4_22 at 25.369 kb on - strand, within Pf1N1B4_22 at 25.369 kb on - strand, within Pf1N1B4_22 at 25.420 kb on + strand, within Pf1N1B4_22 at 25.420 kb on + strand, within Pf1N1B4_22 at 25.421 kb on - strand, within Pf1N1B4_22 at 25.421 kb on - strand, within Pf1N1B4_22 at 25.421 kb on - strand, within Pf1N1B4_22 at 25.421 kb on - strand at 26.171 kb on - strand at 26.185 kb on - strand at 26.185 kb on - strand at 26.185 kb on - strand at 26.339 kb on - strand at 26.339 kb on - strand at 26.350 kb on - strand at 26.423 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1 remove 24,121 + +0.0 24,122 - +0.0 24,122 - +0.0 24,122 - -1.3 24,252 + Pf1N1B4_21 0.12 +0.0 24,253 - Pf1N1B4_21 0.12 +0.0 24,365 - Pf1N1B4_21 0.26 +0.0 24,423 - Pf1N1B4_21 0.33 +0.0 24,423 - -4.5 24,458 - Pf1N1B4_21 0.37 -1.8 24,458 - Pf1N1B4_21 0.37 +0.0 24,458 - Pf1N1B4_21 0.37 -2.8 24,458 - Pf1N1B4_21 0.37 -1.1 24,478 - Pf1N1B4_21 0.39 -1.8 24,775 - Pf1N1B4_21 0.75 -1.0 25,285 + Pf1N1B4_22 0.50 -2.9 25,286 - Pf1N1B4_22 0.50 -3.4 25,286 - Pf1N1B4_22 0.50 -2.0 25,368 + Pf1N1B4_22 0.66 +0.0 25,369 - Pf1N1B4_22 0.66 -2.8 25,369 - Pf1N1B4_22 0.66 -2.8 25,369 - Pf1N1B4_22 0.66 -2.8 25,420 + Pf1N1B4_22 0.75 -3.9 25,420 + Pf1N1B4_22 0.75 +0.0 25,421 - Pf1N1B4_22 0.75 +0.0 25,421 - Pf1N1B4_22 0.75 -3.0 25,421 - Pf1N1B4_22 0.75 -3.5 25,421 - -2.5 26,171 - -0.7 26,185 - -0.7 26,185 - +0.0 26,185 - +0.0 26,339 - -1.0 26,339 - -1.0 26,350 - +0.0 26,423 - +0.0
Or see this region's nucleotide sequence