Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_196

Experiment: Nitrate reduction, replicate A t1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_195 and Pf1N1B4_196 are separated by 335 nucleotidesPf1N1B4_196 and Pf1N1B4_197 overlap by 4 nucleotidesPf1N1B4_197 and Pf1N1B4_198 overlap by 4 nucleotidesPf1N1B4_198 and Pf1N1B4_199 overlap by 4 nucleotides Pf1N1B4_195 - PhnB protein; putative DNA binding 3-demethylubiquinone-9 3-methyltransferase domain protein, at 243,777 to 244,235 _195 Pf1N1B4_196 - FIG137594: Putative iron-regulated membrane protein, at 244,571 to 245,776 _196 Pf1N1B4_197 - FIG139991: Putative thiamine pyrophosphate-requiring enzyme, at 245,773 to 246,315 _197 Pf1N1B4_198 - FIG137877: Hypothetical protein in pyoverdin gene cluster, at 246,312 to 246,632 _198 Pf1N1B4_199 - FIG049111: Hypothetical protein in pyoverdin gene cluster, at 246,629 to 247,204 _199 Position (kb) 244 245 246Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 243.617 kb on + strandat 243.713 kb on + strandat 243.713 kb on + strandat 243.713 kb on + strandat 243.713 kb on + strandat 243.713 kb on + strandat 243.713 kb on + strandat 243.713 kb on + strandat 243.715 kb on + strandat 243.715 kb on + strandat 243.715 kb on + strandat 243.715 kb on + strandat 243.715 kb on + strandat 243.719 kb on + strandat 243.719 kb on + strandat 243.784 kb on + strandat 243.804 kb on + strandat 243.822 kb on + strandat 243.826 kb on + strand, within Pf1N1B4_195at 243.826 kb on + strand, within Pf1N1B4_195at 243.938 kb on + strand, within Pf1N1B4_195at 243.938 kb on + strand, within Pf1N1B4_195at 243.939 kb on - strand, within Pf1N1B4_195at 244.029 kb on + strand, within Pf1N1B4_195at 244.029 kb on + strand, within Pf1N1B4_195at 244.029 kb on + strand, within Pf1N1B4_195at 244.029 kb on + strand, within Pf1N1B4_195at 244.030 kb on - strand, within Pf1N1B4_195at 244.030 kb on - strand, within Pf1N1B4_195at 244.030 kb on - strand, within Pf1N1B4_195at 244.030 kb on - strand, within Pf1N1B4_195at 244.055 kb on + strand, within Pf1N1B4_195at 244.111 kb on - strand, within Pf1N1B4_195at 244.153 kb on + strand, within Pf1N1B4_195at 244.153 kb on + strandat 244.153 kb on + strand, within Pf1N1B4_195at 244.153 kb on + strandat 244.153 kb on + strand, within Pf1N1B4_195at 244.153 kb on + strand, within Pf1N1B4_195at 244.153 kb on + strand, within Pf1N1B4_195at 244.154 kb on - strand, within Pf1N1B4_195at 244.154 kb on - strand, within Pf1N1B4_195at 244.223 kb on + strandat 244.224 kb on - strandat 244.224 kb on - strandat 244.260 kb on + strandat 244.261 kb on - strandat 244.295 kb on + strandat 244.295 kb on + strandat 244.400 kb on + strandat 244.418 kb on - strandat 244.453 kb on + strandat 244.454 kb on - strandat 244.464 kb on + strandat 244.567 kb on - strandat 244.612 kb on + strandat 244.613 kb on - strandat 244.644 kb on + strandat 244.757 kb on + strand, within Pf1N1B4_196at 244.758 kb on - strand, within Pf1N1B4_196at 244.889 kb on + strand, within Pf1N1B4_196at 244.889 kb on + strand, within Pf1N1B4_196at 244.889 kb on + strand, within Pf1N1B4_196at 244.890 kb on - strandat 244.890 kb on - strand, within Pf1N1B4_196at 244.890 kb on - strand, within Pf1N1B4_196at 245.255 kb on - strand, within Pf1N1B4_196at 245.414 kb on + strand, within Pf1N1B4_196at 245.414 kb on + strand, within Pf1N1B4_196at 245.414 kb on + strand, within Pf1N1B4_196at 245.483 kb on + strand, within Pf1N1B4_196at 245.483 kb on + strand, within Pf1N1B4_196at 245.484 kb on - strand, within Pf1N1B4_196at 245.887 kb on + strand, within Pf1N1B4_197at 245.888 kb on - strand, within Pf1N1B4_197at 245.888 kb on - strand, within Pf1N1B4_197at 246.025 kb on + strand, within Pf1N1B4_197at 246.025 kb on + strandat 246.337 kb on + strandat 246.338 kb on - strandat 246.338 kb on - strandat 246.338 kb on - strandat 246.341 kb on - strandat 246.564 kb on + strand, within Pf1N1B4_198at 246.564 kb on + strand, within Pf1N1B4_198at 246.564 kb on + strandat 246.564 kb on + strand, within Pf1N1B4_198at 246.565 kb on - strand, within Pf1N1B4_198at 246.565 kb on - strand, within Pf1N1B4_198at 246.565 kb on - strand, within Pf1N1B4_198at 246.565 kb on - strand, within Pf1N1B4_198at 246.634 kb on + strandat 246.634 kb on + strandat 246.635 kb on - strandat 246.635 kb on - strandat 246.635 kb on - strandat 246.686 kb on + strandat 246.686 kb on + strandat 246.686 kb on + strandat 246.686 kb on + strandat 246.686 kb on + strandat 246.686 kb on + strandat 246.686 kb on + strandat 246.687 kb on - strand, within Pf1N1B4_199at 246.687 kb on - strand, within Pf1N1B4_199at 246.687 kb on - strand, within Pf1N1B4_199at 246.687 kb on - strand, within Pf1N1B4_199at 246.687 kb on - strand, within Pf1N1B4_199at 246.687 kb on - strand, within Pf1N1B4_199at 246.687 kb on - strand, within Pf1N1B4_199

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1
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243,617 + +0.0
243,713 + +0.0
243,713 + +0.0
243,713 + -2.0
243,713 + +0.0
243,713 + +0.0
243,713 + +0.0
243,713 + -2.4
243,715 + -2.5
243,715 + +0.0
243,715 + +0.0
243,715 + -1.4
243,715 + -1.4
243,719 + +0.0
243,719 + +0.0
243,784 + +0.0
243,804 + -2.9
243,822 + +0.0
243,826 + Pf1N1B4_195 0.11 -1.7
243,826 + Pf1N1B4_195 0.11 +0.0
243,938 + Pf1N1B4_195 0.35 +0.0
243,938 + Pf1N1B4_195 0.35 +0.0
243,939 - Pf1N1B4_195 0.35 +0.0
244,029 + Pf1N1B4_195 0.55 +0.0
244,029 + Pf1N1B4_195 0.55 -1.3
244,029 + Pf1N1B4_195 0.55 -2.0
244,029 + Pf1N1B4_195 0.55 +0.0
244,030 - Pf1N1B4_195 0.55 +0.0
244,030 - Pf1N1B4_195 0.55 +0.0
244,030 - Pf1N1B4_195 0.55 -1.3
244,030 - Pf1N1B4_195 0.55 +0.0
244,055 + Pf1N1B4_195 0.61 +0.0
244,111 - Pf1N1B4_195 0.73 +0.0
244,153 + Pf1N1B4_195 0.82 -2.6
244,153 + +0.0
244,153 + Pf1N1B4_195 0.82 +0.0
244,153 + +1.0
244,153 + Pf1N1B4_195 0.82 -3.0
244,153 + Pf1N1B4_195 0.82 -1.8
244,153 + Pf1N1B4_195 0.82 +0.0
244,154 - Pf1N1B4_195 0.82 -2.3
244,154 - Pf1N1B4_195 0.82 +0.0
244,223 + -0.5
244,224 - -1.8
244,224 - -1.6
244,260 + +0.0
244,261 - +0.0
244,295 + -2.5
244,295 + +0.0
244,400 + +0.0
244,418 - +0.0
244,453 + +0.0
244,454 - +0.0
244,464 + +0.0
244,567 - +0.0
244,612 + +0.0
244,613 - -1.1
244,644 + -0.5
244,757 + Pf1N1B4_196 0.15 -5.1
244,758 - Pf1N1B4_196 0.16 +0.0
244,889 + Pf1N1B4_196 0.26 -2.5
244,889 + Pf1N1B4_196 0.26 +0.0
244,889 + Pf1N1B4_196 0.26 +0.0
244,890 - +0.0
244,890 - Pf1N1B4_196 0.26 +0.0
244,890 - Pf1N1B4_196 0.26 +0.0
245,255 - Pf1N1B4_196 0.57 -2.7
245,414 + Pf1N1B4_196 0.70 -2.4
245,414 + Pf1N1B4_196 0.70 -1.0
245,414 + Pf1N1B4_196 0.70 -2.8
245,483 + Pf1N1B4_196 0.76 -2.5
245,483 + Pf1N1B4_196 0.76 +0.0
245,484 - Pf1N1B4_196 0.76 +0.0
245,887 + Pf1N1B4_197 0.21 +0.0
245,888 - Pf1N1B4_197 0.21 -1.2
245,888 - Pf1N1B4_197 0.21 +0.0
246,025 + Pf1N1B4_197 0.46 +0.0
246,025 + +0.0
246,337 + +0.0
246,338 - -0.8
246,338 - -1.1
246,338 - -2.5
246,341 - +0.0
246,564 + Pf1N1B4_198 0.79 -2.8
246,564 + Pf1N1B4_198 0.79 +0.0
246,564 + +0.0
246,564 + Pf1N1B4_198 0.79 +0.0
246,565 - Pf1N1B4_198 0.79 +0.0
246,565 - Pf1N1B4_198 0.79 +0.0
246,565 - Pf1N1B4_198 0.79 +0.0
246,565 - Pf1N1B4_198 0.79 +0.0
246,634 + +0.0
246,634 + -0.6
246,635 - +0.1
246,635 - +0.0
246,635 - +0.0
246,686 + -1.6
246,686 + +0.0
246,686 + +0.0
246,686 + -2.7
246,686 + +0.0
246,686 + +0.0
246,686 + -0.3
246,687 - Pf1N1B4_199 0.10 +0.0
246,687 - Pf1N1B4_199 0.10 +0.0
246,687 - Pf1N1B4_199 0.10 -1.9
246,687 - Pf1N1B4_199 0.10 +0.0
246,687 - Pf1N1B4_199 0.10 +0.0
246,687 - Pf1N1B4_199 0.10 +0.0
246,687 - Pf1N1B4_199 0.10 +0.0

Or see this region's nucleotide sequence