Experiment: Nitrate reduction, replicate A t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1724 and Pf1N1B4_1725 are separated by 3 nucleotides Pf1N1B4_1725 and Pf1N1B4_1726 are separated by 85 nucleotides Pf1N1B4_1726 and Pf1N1B4_1727 are separated by 204 nucleotides
Pf1N1B4_1724 - Sulfate and thiosulfate import ATP-binding protein CysA (EC 3.6.3.25), at 1,970,644 to 1,971,633
_1724
Pf1N1B4_1725 - Sulfate transport system permease protein CysW, at 1,971,637 to 1,972,434
_1725
Pf1N1B4_1726 - Sulfate transport system permease protein CysT, at 1,972,520 to 1,973,338
_1726
Pf1N1B4_1727 - Sulfate and thiosulfate binding protein CysP, at 1,973,543 to 1,974,556
_1727
Position (kb) 1972
1973
1974 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 1971.638 kb on + strand at 1971.638 kb on + strand at 1971.638 kb on + strand at 1971.638 kb on + strand at 1971.638 kb on + strand at 1971.638 kb on + strand at 1971.639 kb on - strand at 1971.639 kb on - strand at 1971.639 kb on - strand at 1971.639 kb on - strand at 1971.639 kb on - strand at 1971.673 kb on - strand at 1971.761 kb on + strand, within Pf1N1B4_1725 at 1971.761 kb on + strand, within Pf1N1B4_1725 at 1971.761 kb on + strand, within Pf1N1B4_1725 at 1971.762 kb on - strand, within Pf1N1B4_1725 at 1971.762 kb on - strand, within Pf1N1B4_1725 at 1971.762 kb on - strand, within Pf1N1B4_1725 at 1971.770 kb on + strand, within Pf1N1B4_1725 at 1971.770 kb on + strand, within Pf1N1B4_1725 at 1971.770 kb on + strand, within Pf1N1B4_1725 at 1971.770 kb on + strand, within Pf1N1B4_1725 at 1971.771 kb on - strand, within Pf1N1B4_1725 at 1971.771 kb on - strand, within Pf1N1B4_1725 at 1971.771 kb on - strand, within Pf1N1B4_1725 at 1971.771 kb on - strand, within Pf1N1B4_1725 at 1971.771 kb on - strand, within Pf1N1B4_1725 at 1971.884 kb on + strand, within Pf1N1B4_1725 at 1971.884 kb on + strand, within Pf1N1B4_1725 at 1971.884 kb on + strand, within Pf1N1B4_1725 at 1971.884 kb on + strand, within Pf1N1B4_1725 at 1971.884 kb on + strand, within Pf1N1B4_1725 at 1971.885 kb on - strand, within Pf1N1B4_1725 at 1971.885 kb on - strand, within Pf1N1B4_1725 at 1971.885 kb on - strand, within Pf1N1B4_1725 at 1972.132 kb on + strand, within Pf1N1B4_1725 at 1972.132 kb on + strand, within Pf1N1B4_1725 at 1972.132 kb on + strand, within Pf1N1B4_1725 at 1972.132 kb on + strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.217 kb on + strand, within Pf1N1B4_1725 at 1972.218 kb on - strand, within Pf1N1B4_1725 at 1972.218 kb on - strand, within Pf1N1B4_1725 at 1972.491 kb on + strand at 1972.491 kb on + strand at 1972.492 kb on - strand at 1972.592 kb on + strand at 1972.592 kb on + strand at 1972.592 kb on + strand at 1972.593 kb on - strand at 1972.593 kb on - strand at 1972.617 kb on + strand, within Pf1N1B4_1726 at 1972.618 kb on - strand, within Pf1N1B4_1726 at 1972.623 kb on + strand, within Pf1N1B4_1726 at 1972.624 kb on - strand, within Pf1N1B4_1726 at 1972.797 kb on - strand, within Pf1N1B4_1726 at 1972.856 kb on + strand, within Pf1N1B4_1726 at 1972.870 kb on + strand, within Pf1N1B4_1726 at 1972.871 kb on - strand, within Pf1N1B4_1726 at 1972.871 kb on - strand at 1972.871 kb on - strand, within Pf1N1B4_1726 at 1972.874 kb on + strand, within Pf1N1B4_1726 at 1972.874 kb on + strand, within Pf1N1B4_1726 at 1972.875 kb on - strand, within Pf1N1B4_1726 at 1972.878 kb on - strand, within Pf1N1B4_1726 at 1972.878 kb on - strand, within Pf1N1B4_1726 at 1972.892 kb on + strand, within Pf1N1B4_1726 at 1972.892 kb on + strand, within Pf1N1B4_1726 at 1972.929 kb on + strand, within Pf1N1B4_1726 at 1972.929 kb on + strand, within Pf1N1B4_1726 at 1972.986 kb on + strand, within Pf1N1B4_1726 at 1972.986 kb on + strand, within Pf1N1B4_1726 at 1972.987 kb on - strand, within Pf1N1B4_1726 at 1973.079 kb on + strand, within Pf1N1B4_1726 at 1973.136 kb on + strand, within Pf1N1B4_1726 at 1973.137 kb on - strand, within Pf1N1B4_1726 at 1973.137 kb on - strand, within Pf1N1B4_1726 at 1973.216 kb on - strand, within Pf1N1B4_1726 at 1973.274 kb on + strand at 1973.274 kb on + strand at 1973.274 kb on + strand at 1973.275 kb on - strand at 1973.275 kb on - strand at 1973.275 kb on - strand at 1973.275 kb on - strand at 1973.275 kb on - strand at 1973.275 kb on - strand at 1973.275 kb on - strand at 1973.286 kb on + strand at 1973.326 kb on + strand at 1973.327 kb on - strand at 1973.327 kb on - strand at 1973.338 kb on + strand at 1973.338 kb on + strand at 1973.338 kb on + strand at 1973.338 kb on + strand at 1973.338 kb on + strand at 1973.338 kb on + strand at 1973.338 kb on + strand at 1973.338 kb on + strand at 1973.338 kb on + strand at 1973.338 kb on + strand at 1973.338 kb on + strand at 1973.338 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.411 kb on - strand at 1973.411 kb on - strand at 1973.411 kb on - strand at 1973.411 kb on - strand at 1973.411 kb on - strand at 1973.411 kb on - strand at 1973.411 kb on - strand at 1973.411 kb on - strand at 1973.418 kb on - strand at 1973.534 kb on + strand at 1973.534 kb on + strand at 1973.534 kb on + strand at 1973.535 kb on - strand at 1973.535 kb on - strand at 1973.535 kb on - strand at 1973.535 kb on - strand at 1973.535 kb on - strand at 1973.556 kb on + strand at 1973.556 kb on + strand at 1973.557 kb on - strand at 1973.557 kb on - strand at 1973.557 kb on - strand at 1973.700 kb on + strand, within Pf1N1B4_1727 at 1973.701 kb on - strand, within Pf1N1B4_1727 at 1973.740 kb on - strand, within Pf1N1B4_1727 at 1973.740 kb on - strand, within Pf1N1B4_1727 at 1973.754 kb on + strand, within Pf1N1B4_1727 at 1974.009 kb on + strand, within Pf1N1B4_1727 at 1974.009 kb on + strand, within Pf1N1B4_1727 at 1974.009 kb on + strand, within Pf1N1B4_1727 at 1974.009 kb on + strand, within Pf1N1B4_1727 at 1974.009 kb on + strand, within Pf1N1B4_1727 at 1974.009 kb on + strand, within Pf1N1B4_1727 at 1974.010 kb on - strand, within Pf1N1B4_1727 at 1974.010 kb on - strand, within Pf1N1B4_1727 at 1974.010 kb on - strand, within Pf1N1B4_1727 at 1974.054 kb on + strand, within Pf1N1B4_1727 at 1974.055 kb on - strand, within Pf1N1B4_1727 at 1974.204 kb on + strand, within Pf1N1B4_1727 at 1974.204 kb on + strand, within Pf1N1B4_1727 at 1974.204 kb on + strand, within Pf1N1B4_1727 at 1974.204 kb on + strand, within Pf1N1B4_1727 at 1974.204 kb on + strand, within Pf1N1B4_1727 at 1974.205 kb on - strand, within Pf1N1B4_1727
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1 remove 1,971,638 + +1.5 1,971,638 + -2.9 1,971,638 + -0.1 1,971,638 + -0.1 1,971,638 + -0.1 1,971,638 + -2.8 1,971,639 - -0.1 1,971,639 - -0.1 1,971,639 - -0.1 1,971,639 - -0.1 1,971,639 - -0.1 1,971,673 - -0.1 1,971,761 + Pf1N1B4_1725 0.16 -3.0 1,971,761 + Pf1N1B4_1725 0.16 -0.1 1,971,761 + Pf1N1B4_1725 0.16 -1.7 1,971,762 - Pf1N1B4_1725 0.16 -0.1 1,971,762 - Pf1N1B4_1725 0.16 -0.1 1,971,762 - Pf1N1B4_1725 0.16 -0.1 1,971,770 + Pf1N1B4_1725 0.17 -3.0 1,971,770 + Pf1N1B4_1725 0.17 -0.1 1,971,770 + Pf1N1B4_1725 0.17 -3.4 1,971,770 + Pf1N1B4_1725 0.17 -2.7 1,971,771 - Pf1N1B4_1725 0.17 -0.1 1,971,771 - Pf1N1B4_1725 0.17 -2.7 1,971,771 - Pf1N1B4_1725 0.17 -0.1 1,971,771 - Pf1N1B4_1725 0.17 -0.1 1,971,771 - Pf1N1B4_1725 0.17 -0.1 1,971,884 + Pf1N1B4_1725 0.31 -0.1 1,971,884 + Pf1N1B4_1725 0.31 -3.3 1,971,884 + Pf1N1B4_1725 0.31 -4.2 1,971,884 + Pf1N1B4_1725 0.31 -3.0 1,971,884 + Pf1N1B4_1725 0.31 -0.1 1,971,885 - Pf1N1B4_1725 0.31 -0.1 1,971,885 - Pf1N1B4_1725 0.31 -0.9 1,971,885 - Pf1N1B4_1725 0.31 -0.1 1,972,132 + Pf1N1B4_1725 0.62 -3.7 1,972,132 + Pf1N1B4_1725 0.62 -0.1 1,972,132 + Pf1N1B4_1725 0.62 -0.1 1,972,132 + Pf1N1B4_1725 0.62 -2.8 1,972,133 - Pf1N1B4_1725 0.62 -0.1 1,972,133 - Pf1N1B4_1725 0.62 -3.2 1,972,133 - Pf1N1B4_1725 0.62 -0.1 1,972,133 - Pf1N1B4_1725 0.62 -3.1 1,972,133 - Pf1N1B4_1725 0.62 -0.1 1,972,133 - Pf1N1B4_1725 0.62 -2.6 1,972,133 - Pf1N1B4_1725 0.62 -0.1 1,972,133 - Pf1N1B4_1725 0.62 -3.5 1,972,133 - Pf1N1B4_1725 0.62 -2.3 1,972,133 - -0.1 1,972,133 - Pf1N1B4_1725 0.62 -3.6 1,972,133 - Pf1N1B4_1725 0.62 -3.2 1,972,133 - Pf1N1B4_1725 0.62 -0.1 1,972,133 - Pf1N1B4_1725 0.62 -0.1 1,972,133 - Pf1N1B4_1725 0.62 -0.1 1,972,133 - Pf1N1B4_1725 0.62 -2.0 1,972,133 - Pf1N1B4_1725 0.62 -2.5 1,972,133 - Pf1N1B4_1725 0.62 -0.1 1,972,133 - Pf1N1B4_1725 0.62 -3.2 1,972,136 + Pf1N1B4_1725 0.63 -0.1 1,972,136 + Pf1N1B4_1725 0.63 -2.9 1,972,136 + Pf1N1B4_1725 0.63 -0.1 1,972,136 + Pf1N1B4_1725 0.63 -0.1 1,972,136 + Pf1N1B4_1725 0.63 -0.1 1,972,136 + Pf1N1B4_1725 0.63 -0.1 1,972,136 + Pf1N1B4_1725 0.63 -0.1 1,972,136 + Pf1N1B4_1725 0.63 -3.7 1,972,136 + Pf1N1B4_1725 0.63 -0.1 1,972,137 - Pf1N1B4_1725 0.63 -0.1 1,972,137 - Pf1N1B4_1725 0.63 -0.1 1,972,137 - Pf1N1B4_1725 0.63 -0.1 1,972,137 - Pf1N1B4_1725 0.63 -2.8 1,972,137 - Pf1N1B4_1725 0.63 -0.1 1,972,137 - Pf1N1B4_1725 0.63 -3.2 1,972,137 - Pf1N1B4_1725 0.63 -0.1 1,972,137 - Pf1N1B4_1725 0.63 -0.1 1,972,137 - Pf1N1B4_1725 0.63 -2.9 1,972,137 - Pf1N1B4_1725 0.63 -3.5 1,972,217 + Pf1N1B4_1725 0.73 -0.1 1,972,218 - Pf1N1B4_1725 0.73 -0.1 1,972,218 - Pf1N1B4_1725 0.73 -2.9 1,972,491 + -0.1 1,972,491 + -0.1 1,972,492 - -0.1 1,972,592 + -0.1 1,972,592 + -0.1 1,972,592 + -0.1 1,972,593 - -4.3 1,972,593 - -0.1 1,972,617 + Pf1N1B4_1726 0.12 -0.1 1,972,618 - Pf1N1B4_1726 0.12 -0.1 1,972,623 + Pf1N1B4_1726 0.13 -0.1 1,972,624 - Pf1N1B4_1726 0.13 -3.5 1,972,797 - Pf1N1B4_1726 0.34 -0.1 1,972,856 + Pf1N1B4_1726 0.41 -0.1 1,972,870 + Pf1N1B4_1726 0.43 -0.1 1,972,871 - Pf1N1B4_1726 0.43 -0.1 1,972,871 - -0.1 1,972,871 - Pf1N1B4_1726 0.43 -2.7 1,972,874 + Pf1N1B4_1726 0.43 -0.1 1,972,874 + Pf1N1B4_1726 0.43 -0.1 1,972,875 - Pf1N1B4_1726 0.43 -0.1 1,972,878 - Pf1N1B4_1726 0.44 -0.1 1,972,878 - Pf1N1B4_1726 0.44 -0.1 1,972,892 + Pf1N1B4_1726 0.45 -0.1 1,972,892 + Pf1N1B4_1726 0.45 -0.1 1,972,929 + Pf1N1B4_1726 0.50 -0.1 1,972,929 + Pf1N1B4_1726 0.50 -0.1 1,972,986 + Pf1N1B4_1726 0.57 -0.1 1,972,986 + Pf1N1B4_1726 0.57 -2.9 1,972,987 - Pf1N1B4_1726 0.57 -3.6 1,973,079 + Pf1N1B4_1726 0.68 -3.4 1,973,136 + Pf1N1B4_1726 0.75 -3.5 1,973,137 - Pf1N1B4_1726 0.75 -0.1 1,973,137 - Pf1N1B4_1726 0.75 -0.1 1,973,216 - Pf1N1B4_1726 0.85 -1.8 1,973,274 + -0.1 1,973,274 + -0.1 1,973,274 + -3.1 1,973,275 - -0.1 1,973,275 - -0.1 1,973,275 - -2.6 1,973,275 - -0.1 1,973,275 - -0.1 1,973,275 - -0.1 1,973,275 - -4.1 1,973,286 + -0.1 1,973,326 + -0.1 1,973,327 - -0.1 1,973,327 - -0.1 1,973,338 + -0.1 1,973,338 + -0.1 1,973,338 + -0.1 1,973,338 + -3.3 1,973,338 + -3.5 1,973,338 + -0.1 1,973,338 + -0.1 1,973,338 + -0.1 1,973,338 + -2.6 1,973,338 + -0.1 1,973,338 + -0.1 1,973,338 + -0.1 1,973,410 + -0.1 1,973,410 + -0.1 1,973,410 + -0.1 1,973,410 + -2.5 1,973,410 + -0.1 1,973,410 + -0.1 1,973,410 + -2.5 1,973,410 + -0.1 1,973,410 + -0.1 1,973,410 + -0.1 1,973,410 + -0.1 1,973,410 + -0.1 1,973,410 + -2.1 1,973,410 + -0.1 1,973,411 - -1.7 1,973,411 - -0.1 1,973,411 - -0.1 1,973,411 - -2.2 1,973,411 - -0.1 1,973,411 - -5.0 1,973,411 - -0.1 1,973,411 - -0.1 1,973,418 - -0.1 1,973,534 + -0.1 1,973,534 + -2.2 1,973,534 + -2.8 1,973,535 - -3.6 1,973,535 - -2.8 1,973,535 - -1.7 1,973,535 - -0.8 1,973,535 - -0.1 1,973,556 + -0.1 1,973,556 + -0.1 1,973,557 - -0.1 1,973,557 - -0.1 1,973,557 - -0.1 1,973,700 + Pf1N1B4_1727 0.15 -0.1 1,973,701 - Pf1N1B4_1727 0.16 -0.1 1,973,740 - Pf1N1B4_1727 0.19 -3.0 1,973,740 - Pf1N1B4_1727 0.19 -0.1 1,973,754 + Pf1N1B4_1727 0.21 -0.1 1,974,009 + Pf1N1B4_1727 0.46 -0.1 1,974,009 + Pf1N1B4_1727 0.46 -0.1 1,974,009 + Pf1N1B4_1727 0.46 -2.0 1,974,009 + Pf1N1B4_1727 0.46 -0.4 1,974,009 + Pf1N1B4_1727 0.46 -0.1 1,974,009 + Pf1N1B4_1727 0.46 -0.1 1,974,010 - Pf1N1B4_1727 0.46 -0.1 1,974,010 - Pf1N1B4_1727 0.46 -0.1 1,974,010 - Pf1N1B4_1727 0.46 -1.5 1,974,054 + Pf1N1B4_1727 0.50 -0.1 1,974,055 - Pf1N1B4_1727 0.50 -0.1 1,974,204 + Pf1N1B4_1727 0.65 -0.1 1,974,204 + Pf1N1B4_1727 0.65 -0.1 1,974,204 + Pf1N1B4_1727 0.65 -0.1 1,974,204 + Pf1N1B4_1727 0.65 -2.6 1,974,204 + Pf1N1B4_1727 0.65 -0.1 1,974,205 - Pf1N1B4_1727 0.65 -1.1
Or see this region's nucleotide sequence