Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1533

Experiment: Nitrate reduction, replicate A t1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1532 and Pf1N1B4_1533 are separated by 246 nucleotidesPf1N1B4_1533 and Pf1N1B4_1534 are separated by 124 nucleotides Pf1N1B4_1532 - Uroporphyrinogen III decarboxylase (EC 4.1.1.37), at 1,763,850 to 1,764,917 _1532 Pf1N1B4_1533 - Glutamate synthase [NADPH] small chain (EC 1.4.1.13), at 1,765,164 to 1,766,582 _1533 Pf1N1B4_1534 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 1,766,707 to 1,771,086 _1534 Position (kb) 1765 1766 1767Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 1765.160 kb on - strandat 1765.160 kb on - strandat 1765.177 kb on + strandat 1765.178 kb on - strandat 1765.295 kb on - strandat 1765.664 kb on + strand, within Pf1N1B4_1533at 1765.664 kb on + strand, within Pf1N1B4_1533at 1765.664 kb on + strand, within Pf1N1B4_1533at 1765.664 kb on + strand, within Pf1N1B4_1533at 1765.664 kb on + strand, within Pf1N1B4_1533at 1765.664 kb on + strand, within Pf1N1B4_1533at 1765.665 kb on - strand, within Pf1N1B4_1533at 1765.665 kb on - strand, within Pf1N1B4_1533at 1765.665 kb on - strand, within Pf1N1B4_1533at 1765.665 kb on - strand, within Pf1N1B4_1533at 1765.665 kb on - strand, within Pf1N1B4_1533at 1765.843 kb on + strand, within Pf1N1B4_1533at 1765.843 kb on + strand, within Pf1N1B4_1533at 1765.843 kb on + strand, within Pf1N1B4_1533at 1765.843 kb on + strand, within Pf1N1B4_1533at 1765.843 kb on + strand, within Pf1N1B4_1533at 1765.843 kb on + strand, within Pf1N1B4_1533at 1765.843 kb on + strand, within Pf1N1B4_1533at 1765.843 kb on + strand, within Pf1N1B4_1533at 1765.844 kb on - strand, within Pf1N1B4_1533at 1765.844 kb on - strand, within Pf1N1B4_1533at 1765.844 kb on - strand, within Pf1N1B4_1533at 1765.844 kb on - strand, within Pf1N1B4_1533at 1765.844 kb on - strand, within Pf1N1B4_1533at 1765.844 kb on - strand, within Pf1N1B4_1533at 1765.844 kb on - strand, within Pf1N1B4_1533at 1765.844 kb on - strand, within Pf1N1B4_1533at 1765.844 kb on - strand, within Pf1N1B4_1533at 1765.844 kb on - strand, within Pf1N1B4_1533at 1765.844 kb on - strand, within Pf1N1B4_1533at 1765.844 kb on - strand, within Pf1N1B4_1533at 1765.849 kb on + strand, within Pf1N1B4_1533at 1765.849 kb on + strand, within Pf1N1B4_1533at 1765.849 kb on + strand, within Pf1N1B4_1533at 1765.849 kb on + strand, within Pf1N1B4_1533at 1765.849 kb on + strand, within Pf1N1B4_1533at 1765.849 kb on + strand, within Pf1N1B4_1533at 1765.849 kb on + strand, within Pf1N1B4_1533at 1765.849 kb on + strand, within Pf1N1B4_1533at 1765.849 kb on + strand, within Pf1N1B4_1533at 1765.849 kb on + strand, within Pf1N1B4_1533at 1765.849 kb on + strand, within Pf1N1B4_1533at 1765.849 kb on + strand, within Pf1N1B4_1533at 1765.850 kb on - strand, within Pf1N1B4_1533at 1765.850 kb on - strand, within Pf1N1B4_1533at 1765.850 kb on - strand, within Pf1N1B4_1533at 1765.850 kb on - strand, within Pf1N1B4_1533at 1765.850 kb on - strand, within Pf1N1B4_1533at 1765.850 kb on - strand, within Pf1N1B4_1533at 1765.850 kb on - strandat 1765.850 kb on - strand, within Pf1N1B4_1533at 1765.879 kb on + strand, within Pf1N1B4_1533at 1765.879 kb on + strand, within Pf1N1B4_1533at 1766.200 kb on + strand, within Pf1N1B4_1533at 1766.200 kb on + strand, within Pf1N1B4_1533at 1766.200 kb on + strand, within Pf1N1B4_1533at 1766.200 kb on + strand, within Pf1N1B4_1533at 1766.201 kb on - strand, within Pf1N1B4_1533at 1766.201 kb on - strand, within Pf1N1B4_1533at 1766.201 kb on - strand, within Pf1N1B4_1533at 1766.201 kb on - strand, within Pf1N1B4_1533at 1766.201 kb on - strand, within Pf1N1B4_1533at 1766.201 kb on - strand, within Pf1N1B4_1533at 1766.479 kb on + strandat 1766.480 kb on - strandat 1766.480 kb on - strandat 1766.480 kb on - strandat 1766.586 kb on + strandat 1766.587 kb on - strandat 1766.587 kb on - strandat 1766.587 kb on - strandat 1766.587 kb on - strandat 1766.681 kb on - strandat 1766.681 kb on - strandat 1766.728 kb on - strandat 1766.728 kb on - strandat 1766.728 kb on - strandat 1766.761 kb on - strandat 1766.789 kb on + strandat 1766.789 kb on + strandat 1766.789 kb on + strandat 1766.790 kb on - strandat 1766.846 kb on + strandat 1766.846 kb on + strandat 1766.846 kb on + strandat 1766.847 kb on - strandat 1766.847 kb on - strandat 1766.847 kb on - strandat 1767.005 kb on + strandat 1767.005 kb on + strandat 1767.006 kb on - strandat 1767.022 kb on + strandat 1767.023 kb on - strandat 1767.047 kb on + strandat 1767.047 kb on + strandat 1767.048 kb on - strandat 1767.048 kb on - strandat 1767.048 kb on - strandat 1767.099 kb on + strandat 1767.099 kb on + strandat 1767.099 kb on + strandat 1767.099 kb on + strandat 1767.203 kb on + strand, within Pf1N1B4_1534at 1767.203 kb on + strand, within Pf1N1B4_1534at 1767.203 kb on + strand, within Pf1N1B4_1534at 1767.203 kb on + strand, within Pf1N1B4_1534at 1767.203 kb on + strand, within Pf1N1B4_1534at 1767.204 kb on - strand, within Pf1N1B4_1534at 1767.204 kb on - strand, within Pf1N1B4_1534at 1767.204 kb on - strand, within Pf1N1B4_1534at 1767.204 kb on - strand, within Pf1N1B4_1534at 1767.204 kb on - strand, within Pf1N1B4_1534at 1767.204 kb on - strand, within Pf1N1B4_1534at 1767.204 kb on - strand, within Pf1N1B4_1534at 1767.204 kb on - strand, within Pf1N1B4_1534at 1767.204 kb on - strand, within Pf1N1B4_1534at 1767.204 kb on - strand, within Pf1N1B4_1534at 1767.204 kb on - strand, within Pf1N1B4_1534at 1767.204 kb on - strand, within Pf1N1B4_1534at 1767.204 kb on - strand, within Pf1N1B4_1534at 1767.204 kb on - strand, within Pf1N1B4_1534at 1767.204 kb on - strand, within Pf1N1B4_1534at 1767.207 kb on + strand, within Pf1N1B4_1534at 1767.207 kb on + strand, within Pf1N1B4_1534at 1767.207 kb on + strand, within Pf1N1B4_1534at 1767.208 kb on - strand, within Pf1N1B4_1534at 1767.208 kb on - strand, within Pf1N1B4_1534at 1767.208 kb on - strand, within Pf1N1B4_1534at 1767.263 kb on + strand, within Pf1N1B4_1534at 1767.263 kb on + strand, within Pf1N1B4_1534at 1767.264 kb on - strand, within Pf1N1B4_1534at 1767.264 kb on - strand, within Pf1N1B4_1534at 1767.264 kb on - strand, within Pf1N1B4_1534at 1767.264 kb on - strand, within Pf1N1B4_1534at 1767.287 kb on + strand, within Pf1N1B4_1534at 1767.287 kb on + strand, within Pf1N1B4_1534at 1767.287 kb on + strandat 1767.287 kb on + strand, within Pf1N1B4_1534at 1767.287 kb on + strand, within Pf1N1B4_1534at 1767.287 kb on + strand, within Pf1N1B4_1534at 1767.287 kb on + strandat 1767.287 kb on + strand, within Pf1N1B4_1534at 1767.288 kb on - strand, within Pf1N1B4_1534at 1767.288 kb on - strand, within Pf1N1B4_1534at 1767.288 kb on - strand, within Pf1N1B4_1534at 1767.288 kb on - strand, within Pf1N1B4_1534at 1767.288 kb on - strand, within Pf1N1B4_1534

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1
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1,765,160 - -0.0
1,765,160 - -1.6
1,765,177 + -0.0
1,765,178 - -3.7
1,765,295 - -2.4
1,765,664 + Pf1N1B4_1533 0.35 -0.0
1,765,664 + Pf1N1B4_1533 0.35 -0.0
1,765,664 + Pf1N1B4_1533 0.35 -0.0
1,765,664 + Pf1N1B4_1533 0.35 -0.0
1,765,664 + Pf1N1B4_1533 0.35 -0.0
1,765,664 + Pf1N1B4_1533 0.35 -0.0
1,765,665 - Pf1N1B4_1533 0.35 -0.0
1,765,665 - Pf1N1B4_1533 0.35 -0.0
1,765,665 - Pf1N1B4_1533 0.35 -0.0
1,765,665 - Pf1N1B4_1533 0.35 -3.3
1,765,665 - Pf1N1B4_1533 0.35 -0.0
1,765,843 + Pf1N1B4_1533 0.48 -0.0
1,765,843 + Pf1N1B4_1533 0.48 -1.9
1,765,843 + Pf1N1B4_1533 0.48 -0.0
1,765,843 + Pf1N1B4_1533 0.48 -0.0
1,765,843 + Pf1N1B4_1533 0.48 -3.5
1,765,843 + Pf1N1B4_1533 0.48 -4.7
1,765,843 + Pf1N1B4_1533 0.48 -0.0
1,765,843 + Pf1N1B4_1533 0.48 -4.8
1,765,844 - Pf1N1B4_1533 0.48 -0.0
1,765,844 - Pf1N1B4_1533 0.48 -0.0
1,765,844 - Pf1N1B4_1533 0.48 -0.0
1,765,844 - Pf1N1B4_1533 0.48 -2.8
1,765,844 - Pf1N1B4_1533 0.48 -5.7
1,765,844 - Pf1N1B4_1533 0.48 -0.0
1,765,844 - Pf1N1B4_1533 0.48 -2.9
1,765,844 - Pf1N1B4_1533 0.48 -0.0
1,765,844 - Pf1N1B4_1533 0.48 -0.0
1,765,844 - Pf1N1B4_1533 0.48 -4.1
1,765,844 - Pf1N1B4_1533 0.48 -0.0
1,765,844 - Pf1N1B4_1533 0.48 -0.0
1,765,849 + Pf1N1B4_1533 0.48 -3.4
1,765,849 + Pf1N1B4_1533 0.48 -1.0
1,765,849 + Pf1N1B4_1533 0.48 -0.0
1,765,849 + Pf1N1B4_1533 0.48 -3.5
1,765,849 + Pf1N1B4_1533 0.48 -1.8
1,765,849 + Pf1N1B4_1533 0.48 -0.0
1,765,849 + Pf1N1B4_1533 0.48 -0.0
1,765,849 + Pf1N1B4_1533 0.48 -0.0
1,765,849 + Pf1N1B4_1533 0.48 -0.0
1,765,849 + Pf1N1B4_1533 0.48 -0.0
1,765,849 + Pf1N1B4_1533 0.48 -0.0
1,765,849 + Pf1N1B4_1533 0.48 -0.0
1,765,850 - Pf1N1B4_1533 0.48 -0.0
1,765,850 - Pf1N1B4_1533 0.48 -3.5
1,765,850 - Pf1N1B4_1533 0.48 -4.2
1,765,850 - Pf1N1B4_1533 0.48 -0.0
1,765,850 - Pf1N1B4_1533 0.48 -3.0
1,765,850 - Pf1N1B4_1533 0.48 -0.0
1,765,850 - -0.0
1,765,850 - Pf1N1B4_1533 0.48 -0.0
1,765,879 + Pf1N1B4_1533 0.50 -0.0
1,765,879 + Pf1N1B4_1533 0.50 -0.0
1,766,200 + Pf1N1B4_1533 0.73 -0.0
1,766,200 + Pf1N1B4_1533 0.73 -0.0
1,766,200 + Pf1N1B4_1533 0.73 -3.4
1,766,200 + Pf1N1B4_1533 0.73 -3.7
1,766,201 - Pf1N1B4_1533 0.73 -0.0
1,766,201 - Pf1N1B4_1533 0.73 -0.0
1,766,201 - Pf1N1B4_1533 0.73 -0.0
1,766,201 - Pf1N1B4_1533 0.73 -0.0
1,766,201 - Pf1N1B4_1533 0.73 -1.4
1,766,201 - Pf1N1B4_1533 0.73 -0.0
1,766,479 + -2.9
1,766,480 - -0.0
1,766,480 - -4.5
1,766,480 - -4.9
1,766,586 + -0.0
1,766,587 - -0.0
1,766,587 - -3.3
1,766,587 - -0.0
1,766,587 - -3.0
1,766,681 - -0.0
1,766,681 - -2.7
1,766,728 - -0.0
1,766,728 - -0.0
1,766,728 - -1.9
1,766,761 - -0.0
1,766,789 + -0.0
1,766,789 + -2.8
1,766,789 + -0.0
1,766,790 - -3.1
1,766,846 + -0.0
1,766,846 + -0.0
1,766,846 + -0.0
1,766,847 - -0.0
1,766,847 - -4.1
1,766,847 - -3.5
1,767,005 + -3.2
1,767,005 + -0.0
1,767,006 - -0.9
1,767,022 + -3.0
1,767,023 - -3.0
1,767,047 + -0.0
1,767,047 + -0.0
1,767,048 - -0.0
1,767,048 - -0.0
1,767,048 - -3.3
1,767,099 + -0.0
1,767,099 + -0.0
1,767,099 + -2.5
1,767,099 + -2.5
1,767,203 + Pf1N1B4_1534 0.11 -3.0
1,767,203 + Pf1N1B4_1534 0.11 -0.0
1,767,203 + Pf1N1B4_1534 0.11 -0.0
1,767,203 + Pf1N1B4_1534 0.11 -3.2
1,767,203 + Pf1N1B4_1534 0.11 -0.0
1,767,204 - Pf1N1B4_1534 0.11 -0.0
1,767,204 - Pf1N1B4_1534 0.11 -3.3
1,767,204 - Pf1N1B4_1534 0.11 -0.0
1,767,204 - Pf1N1B4_1534 0.11 -0.0
1,767,204 - Pf1N1B4_1534 0.11 -0.0
1,767,204 - Pf1N1B4_1534 0.11 -0.0
1,767,204 - Pf1N1B4_1534 0.11 -3.9
1,767,204 - Pf1N1B4_1534 0.11 -2.1
1,767,204 - Pf1N1B4_1534 0.11 -0.0
1,767,204 - Pf1N1B4_1534 0.11 -0.0
1,767,204 - Pf1N1B4_1534 0.11 -0.0
1,767,204 - Pf1N1B4_1534 0.11 -0.0
1,767,204 - Pf1N1B4_1534 0.11 -0.0
1,767,204 - Pf1N1B4_1534 0.11 -0.0
1,767,204 - Pf1N1B4_1534 0.11 -3.9
1,767,207 + Pf1N1B4_1534 0.11 -2.6
1,767,207 + Pf1N1B4_1534 0.11 -0.0
1,767,207 + Pf1N1B4_1534 0.11 -4.1
1,767,208 - Pf1N1B4_1534 0.11 -0.0
1,767,208 - Pf1N1B4_1534 0.11 -0.0
1,767,208 - Pf1N1B4_1534 0.11 -0.0
1,767,263 + Pf1N1B4_1534 0.13 -1.3
1,767,263 + Pf1N1B4_1534 0.13 -0.0
1,767,264 - Pf1N1B4_1534 0.13 -3.0
1,767,264 - Pf1N1B4_1534 0.13 -0.0
1,767,264 - Pf1N1B4_1534 0.13 -0.0
1,767,264 - Pf1N1B4_1534 0.13 -0.0
1,767,287 + Pf1N1B4_1534 0.13 -3.8
1,767,287 + Pf1N1B4_1534 0.13 -2.2
1,767,287 + -0.0
1,767,287 + Pf1N1B4_1534 0.13 -4.1
1,767,287 + Pf1N1B4_1534 0.13 -0.0
1,767,287 + Pf1N1B4_1534 0.13 -0.0
1,767,287 + -1.0
1,767,287 + Pf1N1B4_1534 0.13 -4.4
1,767,288 - Pf1N1B4_1534 0.13 -0.0
1,767,288 - Pf1N1B4_1534 0.13 -0.0
1,767,288 - Pf1N1B4_1534 0.13 -0.0
1,767,288 - Pf1N1B4_1534 0.13 -2.9
1,767,288 - Pf1N1B4_1534 0.13 -0.0

Or see this region's nucleotide sequence