Experiment: Nitrate reduction, replicate A t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1078 and Pf1N1B4_1079 overlap by 1 nucleotides Pf1N1B4_1079 and Pf1N1B4_1080 are separated by 209 nucleotides Pf1N1B4_1080 and Pf1N1B4_1081 are separated by 79 nucleotides Pf1N1B4_1081 and Pf1N1B4_1082 are separated by 54 nucleotides Pf1N1B4_1082 and Pf1N1B4_1083 are separated by 2 nucleotides
Pf1N1B4_1078 - LptA, protein essential for LPS transport across the periplasm, at 1,210,749 to 1,211,240
_1078
Pf1N1B4_1079 - Lipopolysaccharide ABC transporter, ATP-binding protein LptB, at 1,211,240 to 1,211,965
_1079
Pf1N1B4_1080 - RNA polymerase sigma-54 factor RpoN, at 1,212,175 to 1,213,644
_1080
Pf1N1B4_1081 - Ribosome hibernation protein YhbH, at 1,213,724 to 1,214,032
_1081
Pf1N1B4_1082 - PTS IIA-like nitrogen-regulatory protein PtsN, at 1,214,087 to 1,214,509
_1082
Pf1N1B4_1083 - Hypothetical ATP-binding protein UPF0042, contains P-loop, at 1,214,512 to 1,215,369
_1083
Position (kb) 1212
1213
1214 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1211.964 kb on - strand at 1211.964 kb on - strand at 1211.964 kb on - strand at 1212.009 kb on - strand at 1212.027 kb on + strand at 1212.027 kb on + strand at 1212.028 kb on - strand at 1212.033 kb on + strand at 1212.033 kb on + strand at 1212.034 kb on - strand at 1212.044 kb on - strand at 1212.044 kb on - strand at 1212.044 kb on - strand at 1212.071 kb on - strand at 1212.071 kb on - strand at 1212.103 kb on + strand at 1212.131 kb on + strand at 1212.139 kb on + strand at 1213.696 kb on + strand at 1213.696 kb on + strand at 1213.723 kb on + strand at 1213.723 kb on + strand at 1213.723 kb on + strand at 1213.778 kb on + strand at 1213.778 kb on + strand, within Pf1N1B4_1081 at 1213.778 kb on + strand, within Pf1N1B4_1081 at 1213.888 kb on + strand, within Pf1N1B4_1081 at 1213.888 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand at 1213.934 kb on + strand at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand at 1213.934 kb on + strand, within Pf1N1B4_1081
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1 remove 1,211,964 - -0.0 1,211,964 - -2.4 1,211,964 - -2.8 1,212,009 - -2.0 1,212,027 + -0.0 1,212,027 + -0.2 1,212,028 - -0.0 1,212,033 + -0.0 1,212,033 + -0.0 1,212,034 - -0.0 1,212,044 - -0.0 1,212,044 - -0.0 1,212,044 - -3.5 1,212,071 - -0.0 1,212,071 - -1.0 1,212,103 + -0.0 1,212,131 + -0.0 1,212,139 + -0.0 1,213,696 + -0.0 1,213,696 + -0.0 1,213,723 + -4.1 1,213,723 + -0.0 1,213,723 + -3.3 1,213,778 + -0.0 1,213,778 + Pf1N1B4_1081 0.17 -0.0 1,213,778 + Pf1N1B4_1081 0.17 -3.8 1,213,888 + Pf1N1B4_1081 0.53 -0.0 1,213,888 + Pf1N1B4_1081 0.53 -0.0 1,213,934 + -0.0 1,213,934 + Pf1N1B4_1081 0.68 -0.0 1,213,934 + Pf1N1B4_1081 0.68 -2.2 1,213,934 + Pf1N1B4_1081 0.68 -0.0 1,213,934 + Pf1N1B4_1081 0.68 -0.0 1,213,934 + Pf1N1B4_1081 0.68 -2.8 1,213,934 + Pf1N1B4_1081 0.68 -0.0 1,213,934 + Pf1N1B4_1081 0.68 -0.0 1,213,934 + Pf1N1B4_1081 0.68 -0.0 1,213,934 + Pf1N1B4_1081 0.68 -0.0 1,213,934 + Pf1N1B4_1081 0.68 -0.0 1,213,934 + Pf1N1B4_1081 0.68 -0.0 1,213,934 + Pf1N1B4_1081 0.68 -0.0 1,213,934 + Pf1N1B4_1081 0.68 -4.1 1,213,934 + -3.6 1,213,934 + -0.0 1,213,934 + Pf1N1B4_1081 0.68 -2.4 1,213,934 + Pf1N1B4_1081 0.68 -0.0 1,213,934 + Pf1N1B4_1081 0.68 -0.0 1,213,934 + Pf1N1B4_1081 0.68 -0.0 1,213,934 + Pf1N1B4_1081 0.68 -0.0 1,213,934 + Pf1N1B4_1081 0.68 -0.0 1,213,934 + -0.0 1,213,934 + Pf1N1B4_1081 0.68 -3.5
Or see this region's nucleotide sequence