Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1079

Experiment: Nitrate reduction, replicate A t1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1077 and Pf1N1B4_1078 are separated by 43 nucleotidesPf1N1B4_1078 and Pf1N1B4_1079 overlap by 1 nucleotidesPf1N1B4_1079 and Pf1N1B4_1080 are separated by 209 nucleotides Pf1N1B4_1077 - Uncharacterized protein YrbK clustered with lipopolysaccharide transporters, at 1,210,133 to 1,210,705 _1077 Pf1N1B4_1078 - LptA, protein essential for LPS transport across the periplasm, at 1,210,749 to 1,211,240 _1078 Pf1N1B4_1079 - Lipopolysaccharide ABC transporter, ATP-binding protein LptB, at 1,211,240 to 1,211,965 _1079 Pf1N1B4_1080 - RNA polymerase sigma-54 factor RpoN, at 1,212,175 to 1,213,644 _1080 Position (kb) 1211 1212Strain fitness (log2 ratio) -3 -2 -1 0 1at 1210.680 kb on + strandat 1211.964 kb on - strandat 1211.964 kb on - strandat 1211.964 kb on - strandat 1212.009 kb on - strandat 1212.027 kb on + strandat 1212.027 kb on + strandat 1212.028 kb on - strandat 1212.033 kb on + strandat 1212.033 kb on + strandat 1212.034 kb on - strandat 1212.044 kb on - strandat 1212.044 kb on - strandat 1212.044 kb on - strandat 1212.071 kb on - strandat 1212.071 kb on - strandat 1212.103 kb on + strandat 1212.131 kb on + strandat 1212.139 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1
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1,210,680 + -1.2
1,211,964 - -0.0
1,211,964 - -2.4
1,211,964 - -2.8
1,212,009 - -2.0
1,212,027 + -0.0
1,212,027 + -0.2
1,212,028 - -0.0
1,212,033 + -0.0
1,212,033 + -0.0
1,212,034 - -0.0
1,212,044 - -0.0
1,212,044 - -0.0
1,212,044 - -3.5
1,212,071 - -0.0
1,212,071 - -1.0
1,212,103 + -0.0
1,212,131 + -0.0
1,212,139 + -0.0

Or see this region's nucleotide sequence