Experiment: L-Methionine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c21250 and PGA1_c21260 are separated by 14 nucleotides PGA1_c21260 and PGA1_c21270 are separated by 250 nucleotides PGA1_c21270 and PGA1_c21280 are separated by 172 nucleotides
PGA1_c21250: PGA1_c21250 - aspartate aminotransferase AatA, at 2,198,608 to 2,199,810
_c21250
PGA1_c21260: PGA1_c21260 - hypothetical protein, at 2,199,825 to 2,200,247
_c21260
PGA1_c21270: PGA1_c21270 - hypothetical protein, at 2,200,498 to 2,201,364
_c21270
PGA1_c21280: PGA1_c21280 - transcriptional regulator, LysR family, at 2,201,537 to 2,202,451
_c21280
Position (kb)
2200
2201
2202 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2199.517 kb on - strand, within PGA1_c21250 at 2199.522 kb on - strand, within PGA1_c21250 at 2199.548 kb on - strand, within PGA1_c21250 at 2199.621 kb on + strand, within PGA1_c21250 at 2199.807 kb on - strand at 2199.807 kb on - strand at 2199.809 kb on + strand at 2199.817 kb on - strand at 2199.817 kb on - strand at 2199.817 kb on - strand at 2199.817 kb on - strand at 2199.842 kb on - strand at 2199.845 kb on - strand at 2199.996 kb on - strand, within PGA1_c21260 at 2200.052 kb on + strand, within PGA1_c21260 at 2200.084 kb on + strand, within PGA1_c21260 at 2200.107 kb on + strand, within PGA1_c21260 at 2200.120 kb on - strand, within PGA1_c21260 at 2200.168 kb on + strand, within PGA1_c21260 at 2200.175 kb on + strand, within PGA1_c21260 at 2200.185 kb on + strand, within PGA1_c21260 at 2200.241 kb on + strand at 2200.241 kb on + strand at 2200.309 kb on + strand at 2200.309 kb on + strand at 2200.317 kb on - strand at 2200.317 kb on - strand at 2200.328 kb on - strand at 2200.340 kb on + strand at 2200.377 kb on - strand at 2200.436 kb on - strand at 2200.436 kb on - strand at 2200.445 kb on + strand at 2200.445 kb on + strand at 2200.445 kb on + strand at 2200.445 kb on + strand at 2200.453 kb on - strand at 2200.453 kb on - strand at 2200.453 kb on - strand at 2200.468 kb on + strand at 2200.470 kb on + strand at 2200.478 kb on - strand at 2200.497 kb on - strand at 2200.534 kb on + strand at 2200.534 kb on + strand at 2200.534 kb on + strand at 2200.539 kb on + strand at 2200.542 kb on - strand at 2200.626 kb on - strand, within PGA1_c21270 at 2200.737 kb on + strand, within PGA1_c21270 at 2200.748 kb on - strand, within PGA1_c21270 at 2200.813 kb on + strand, within PGA1_c21270 at 2200.818 kb on + strand, within PGA1_c21270 at 2200.819 kb on + strand, within PGA1_c21270 at 2200.821 kb on - strand, within PGA1_c21270 at 2200.821 kb on - strand, within PGA1_c21270 at 2200.978 kb on - strand, within PGA1_c21270 at 2201.040 kb on + strand, within PGA1_c21270 at 2201.048 kb on - strand, within PGA1_c21270 at 2201.061 kb on + strand, within PGA1_c21270 at 2201.069 kb on - strand, within PGA1_c21270 at 2201.155 kb on - strand, within PGA1_c21270 at 2201.162 kb on + strand, within PGA1_c21270 at 2201.170 kb on - strand, within PGA1_c21270 at 2201.185 kb on + strand, within PGA1_c21270 at 2201.219 kb on + strand, within PGA1_c21270 at 2201.225 kb on - strand, within PGA1_c21270 at 2201.227 kb on - strand, within PGA1_c21270 at 2201.232 kb on + strand, within PGA1_c21270 at 2201.331 kb on - strand at 2201.461 kb on + strand at 2201.471 kb on + strand at 2201.471 kb on + strand at 2201.479 kb on - strand at 2201.479 kb on - strand at 2201.497 kb on - strand at 2201.523 kb on - strand at 2201.543 kb on - strand at 2201.550 kb on - strand at 2201.560 kb on - strand at 2201.563 kb on - strand at 2201.570 kb on + strand at 2201.676 kb on - strand, within PGA1_c21280 at 2201.708 kb on + strand, within PGA1_c21280 at 2201.726 kb on - strand, within PGA1_c21280 at 2201.749 kb on + strand, within PGA1_c21280 at 2201.772 kb on + strand, within PGA1_c21280 at 2201.781 kb on - strand, within PGA1_c21280 at 2201.782 kb on + strand, within PGA1_c21280 at 2201.807 kb on + strand, within PGA1_c21280 at 2201.829 kb on + strand, within PGA1_c21280 at 2201.855 kb on + strand, within PGA1_c21280 at 2201.863 kb on - strand, within PGA1_c21280 at 2201.863 kb on - strand, within PGA1_c21280 at 2201.934 kb on + strand, within PGA1_c21280 at 2201.934 kb on + strand, within PGA1_c21280 at 2201.942 kb on + strand, within PGA1_c21280 at 2201.942 kb on - strand, within PGA1_c21280 at 2201.948 kb on - strand, within PGA1_c21280 at 2202.032 kb on - strand, within PGA1_c21280 at 2202.117 kb on - strand, within PGA1_c21280 at 2202.190 kb on - strand, within PGA1_c21280 at 2202.208 kb on + strand, within PGA1_c21280 at 2202.244 kb on - strand, within PGA1_c21280 at 2202.310 kb on + strand, within PGA1_c21280 at 2202.337 kb on + strand, within PGA1_c21280 at 2202.340 kb on - strand, within PGA1_c21280 at 2202.342 kb on + strand, within PGA1_c21280 at 2202.345 kb on - strand, within PGA1_c21280 at 2202.363 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (N) remove 2,199,517 - PGA1_c21250 0.76 -0.6 2,199,522 - PGA1_c21250 0.76 +0.8 2,199,548 - PGA1_c21250 0.78 -0.7 2,199,621 + PGA1_c21250 0.84 -1.7 2,199,807 - -2.3 2,199,807 - -1.7 2,199,809 + -1.5 2,199,817 - -1.4 2,199,817 - -0.6 2,199,817 - +0.0 2,199,817 - +0.4 2,199,842 - -0.0 2,199,845 - +0.6 2,199,996 - PGA1_c21260 0.40 +0.4 2,200,052 + PGA1_c21260 0.54 +0.0 2,200,084 + PGA1_c21260 0.61 -2.3 2,200,107 + PGA1_c21260 0.67 +0.7 2,200,120 - PGA1_c21260 0.70 -0.7 2,200,168 + PGA1_c21260 0.81 +1.3 2,200,175 + PGA1_c21260 0.83 +0.0 2,200,185 + PGA1_c21260 0.85 -0.6 2,200,241 + +0.8 2,200,241 + -0.7 2,200,309 + -0.2 2,200,309 + +0.4 2,200,317 - +0.2 2,200,317 - +1.1 2,200,328 - +0.6 2,200,340 + +0.8 2,200,377 - -1.4 2,200,436 - +1.2 2,200,436 - +0.4 2,200,445 + +0.0 2,200,445 + +0.4 2,200,445 + +0.6 2,200,445 + +0.7 2,200,453 - -0.3 2,200,453 - +0.3 2,200,453 - +0.1 2,200,468 + +1.5 2,200,470 + +0.2 2,200,478 - -0.4 2,200,497 - +1.3 2,200,534 + -0.6 2,200,534 + -0.4 2,200,534 + +0.2 2,200,539 + +0.1 2,200,542 - +0.7 2,200,626 - PGA1_c21270 0.15 +0.4 2,200,737 + PGA1_c21270 0.28 +0.4 2,200,748 - PGA1_c21270 0.29 -0.6 2,200,813 + PGA1_c21270 0.36 +0.6 2,200,818 + PGA1_c21270 0.37 +1.0 2,200,819 + PGA1_c21270 0.37 -0.7 2,200,821 - PGA1_c21270 0.37 +0.4 2,200,821 - PGA1_c21270 0.37 +0.3 2,200,978 - PGA1_c21270 0.55 -0.2 2,201,040 + PGA1_c21270 0.63 -0.3 2,201,048 - PGA1_c21270 0.63 +0.1 2,201,061 + PGA1_c21270 0.65 +1.4 2,201,069 - PGA1_c21270 0.66 +0.8 2,201,155 - PGA1_c21270 0.76 -0.6 2,201,162 + PGA1_c21270 0.77 +0.8 2,201,170 - PGA1_c21270 0.78 +0.5 2,201,185 + PGA1_c21270 0.79 -0.7 2,201,219 + PGA1_c21270 0.83 +0.1 2,201,225 - PGA1_c21270 0.84 +1.0 2,201,227 - PGA1_c21270 0.84 +1.4 2,201,232 + PGA1_c21270 0.85 +0.3 2,201,331 - -1.4 2,201,461 + +0.9 2,201,471 + +0.2 2,201,471 + +0.0 2,201,479 - -1.8 2,201,479 - +0.2 2,201,497 - -1.4 2,201,523 - +3.2 2,201,543 - +0.9 2,201,550 - +0.0 2,201,560 - -0.3 2,201,563 - -0.3 2,201,570 + +0.2 2,201,676 - PGA1_c21280 0.15 -0.2 2,201,708 + PGA1_c21280 0.19 -0.9 2,201,726 - PGA1_c21280 0.21 +0.6 2,201,749 + PGA1_c21280 0.23 -1.2 2,201,772 + PGA1_c21280 0.26 +0.9 2,201,781 - PGA1_c21280 0.27 +0.2 2,201,782 + PGA1_c21280 0.27 +0.9 2,201,807 + PGA1_c21280 0.30 +0.8 2,201,829 + PGA1_c21280 0.32 +1.0 2,201,855 + PGA1_c21280 0.35 +0.1 2,201,863 - PGA1_c21280 0.36 -0.4 2,201,863 - PGA1_c21280 0.36 +0.0 2,201,934 + PGA1_c21280 0.43 -1.6 2,201,934 + PGA1_c21280 0.43 -0.3 2,201,942 + PGA1_c21280 0.44 -0.5 2,201,942 - PGA1_c21280 0.44 +1.0 2,201,948 - PGA1_c21280 0.45 +1.6 2,202,032 - PGA1_c21280 0.54 +0.1 2,202,117 - PGA1_c21280 0.63 +0.4 2,202,190 - PGA1_c21280 0.71 -0.3 2,202,208 + PGA1_c21280 0.73 +0.5 2,202,244 - PGA1_c21280 0.77 +0.0 2,202,310 + PGA1_c21280 0.84 +0.7 2,202,337 + PGA1_c21280 0.87 +0.2 2,202,340 - PGA1_c21280 0.88 -1.3 2,202,342 + PGA1_c21280 0.88 +0.4 2,202,345 - PGA1_c21280 0.88 -0.3 2,202,363 + -0.7
Or see this region's nucleotide sequence