Experiment: BHIS with Sulfamonomethoxine 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4013 and BT4014 are separated by 6 nucleotides BT4014 and BT4015 are separated by 281 nucleotides
BT4013: BT4013 - restriction endonuclease (NCBI ptt file), at 5,228,099 to 5,232,097
BT4013
BT4014: BT4014 - putative type II restriction endonuclease (NCBI ptt file), at 5,232,104 to 5,233,120
BT4014
BT4015: BT4015 - transposase (NCBI ptt file), at 5,233,402 to 5,234,610
BT4015
Position (kb) 5232
5233
5234 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 5231.352 kb on + strand, within BT4013 at 5231.442 kb on + strand, within BT4013 at 5231.442 kb on + strand, within BT4013 at 5231.448 kb on - strand, within BT4013 at 5231.497 kb on + strand, within BT4013 at 5231.497 kb on + strand, within BT4013 at 5231.497 kb on + strand, within BT4013 at 5231.593 kb on + strand, within BT4013 at 5231.652 kb on - strand, within BT4013 at 5231.885 kb on - strand at 5231.887 kb on - strand at 5231.889 kb on - strand at 5231.889 kb on - strand at 5231.889 kb on - strand at 5231.906 kb on - strand at 5232.032 kb on - strand at 5232.060 kb on + strand at 5232.110 kb on + strand at 5232.335 kb on + strand, within BT4014 at 5232.354 kb on + strand, within BT4014 at 5232.363 kb on + strand, within BT4014 at 5232.452 kb on + strand, within BT4014 at 5232.452 kb on + strand, within BT4014 at 5232.452 kb on + strand, within BT4014 at 5232.480 kb on + strand, within BT4014 at 5232.490 kb on + strand, within BT4014 at 5232.490 kb on + strand, within BT4014 at 5232.554 kb on + strand, within BT4014 at 5232.554 kb on + strand, within BT4014 at 5232.569 kb on + strand, within BT4014 at 5232.678 kb on + strand, within BT4014 at 5232.736 kb on + strand, within BT4014 at 5232.736 kb on + strand, within BT4014 at 5232.736 kb on + strand, within BT4014 at 5232.759 kb on + strand, within BT4014 at 5232.762 kb on + strand, within BT4014 at 5232.827 kb on + strand, within BT4014 at 5232.827 kb on + strand, within BT4014 at 5232.913 kb on + strand, within BT4014 at 5232.913 kb on + strand, within BT4014 at 5232.929 kb on + strand, within BT4014 at 5232.929 kb on + strand, within BT4014 at 5232.979 kb on + strand, within BT4014 at 5233.036 kb on + strand at 5233.036 kb on + strand at 5233.102 kb on + strand at 5233.123 kb on + strand at 5233.123 kb on + strand at 5233.156 kb on - strand at 5233.166 kb on - strand at 5233.202 kb on - strand at 5233.222 kb on + strand at 5233.223 kb on - strand at 5233.350 kb on + strand at 5233.353 kb on + strand at 5233.409 kb on - strand at 5233.447 kb on - strand at 5233.466 kb on + strand at 5233.467 kb on - strand at 5233.521 kb on + strand at 5233.530 kb on + strand, within BT4015 at 5233.572 kb on - strand, within BT4015 at 5233.700 kb on + strand, within BT4015 at 5233.700 kb on + strand, within BT4015 at 5233.701 kb on - strand, within BT4015 at 5233.721 kb on + strand, within BT4015 at 5233.743 kb on + strand, within BT4015 at 5233.832 kb on + strand, within BT4015 at 5233.833 kb on - strand, within BT4015 at 5233.863 kb on + strand, within BT4015 at 5233.895 kb on + strand, within BT4015 at 5233.944 kb on + strand, within BT4015 at 5234.048 kb on + strand, within BT4015 at 5234.049 kb on - strand, within BT4015 at 5234.065 kb on - strand, within BT4015 at 5234.072 kb on + strand, within BT4015 at 5234.072 kb on + strand, within BT4015 at 5234.080 kb on + strand, within BT4015 at 5234.080 kb on + strand, within BT4015 at 5234.114 kb on + strand, within BT4015
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Sulfamonomethoxine 0.2 mM remove 5,231,352 + BT4013 0.81 -1.0 5,231,442 + BT4013 0.84 +0.0 5,231,442 + BT4013 0.84 +0.0 5,231,448 - BT4013 0.84 -1.8 5,231,497 + BT4013 0.85 -2.6 5,231,497 + BT4013 0.85 +0.0 5,231,497 + BT4013 0.85 -2.0 5,231,593 + BT4013 0.87 +1.6 5,231,652 - BT4013 0.89 -3.6 5,231,885 - -1.4 5,231,887 - -0.7 5,231,889 - -1.0 5,231,889 - -1.0 5,231,889 - -2.7 5,231,906 - -0.6 5,232,032 - -1.0 5,232,060 + +0.0 5,232,110 + +0.0 5,232,335 + BT4014 0.23 +0.0 5,232,354 + BT4014 0.25 +2.3 5,232,363 + BT4014 0.25 -1.0 5,232,452 + BT4014 0.34 -1.3 5,232,452 + BT4014 0.34 -0.8 5,232,452 + BT4014 0.34 +0.8 5,232,480 + BT4014 0.37 +0.0 5,232,490 + BT4014 0.38 -1.0 5,232,490 + BT4014 0.38 +0.7 5,232,554 + BT4014 0.44 -0.5 5,232,554 + BT4014 0.44 -0.8 5,232,569 + BT4014 0.46 +1.0 5,232,678 + BT4014 0.56 -0.6 5,232,736 + BT4014 0.62 -3.0 5,232,736 + BT4014 0.62 +0.6 5,232,736 + BT4014 0.62 -0.4 5,232,759 + BT4014 0.64 -2.0 5,232,762 + BT4014 0.65 -1.5 5,232,827 + BT4014 0.71 -2.3 5,232,827 + BT4014 0.71 +2.0 5,232,913 + BT4014 0.80 +0.2 5,232,913 + BT4014 0.80 -1.6 5,232,929 + BT4014 0.81 -1.0 5,232,929 + BT4014 0.81 +1.6 5,232,979 + BT4014 0.86 +3.8 5,233,036 + -0.5 5,233,036 + +0.0 5,233,102 + -1.0 5,233,123 + +1.0 5,233,123 + -3.3 5,233,156 - +2.0 5,233,166 - -1.5 5,233,202 - -2.8 5,233,222 + +0.0 5,233,223 - +0.4 5,233,350 + -0.4 5,233,353 + +0.0 5,233,409 - +1.0 5,233,447 - -1.7 5,233,466 + +1.6 5,233,467 - +0.5 5,233,521 + -2.6 5,233,530 + BT4015 0.11 -0.6 5,233,572 - BT4015 0.14 -2.3 5,233,700 + BT4015 0.25 +1.0 5,233,700 + BT4015 0.25 -1.9 5,233,701 - BT4015 0.25 -1.7 5,233,721 + BT4015 0.26 +1.0 5,233,743 + BT4015 0.28 -1.5 5,233,832 + BT4015 0.36 -1.0 5,233,833 - BT4015 0.36 -3.0 5,233,863 + BT4015 0.38 -2.0 5,233,895 + BT4015 0.41 -1.0 5,233,944 + BT4015 0.45 -1.7 5,234,048 + BT4015 0.53 -1.0 5,234,049 - BT4015 0.54 +1.8 5,234,065 - BT4015 0.55 -1.2 5,234,072 + BT4015 0.55 -2.2 5,234,072 + BT4015 0.55 +0.0 5,234,080 + BT4015 0.56 -1.6 5,234,080 + BT4015 0.56 -0.7 5,234,114 + BT4015 0.59 -3.4
Or see this region's nucleotide sequence