Experiment: MoLS4 with N2 gas (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gcvPA and gcvH are separated by 14 nucleotides gcvH and DVU1427 are separated by 191 nucleotides DVU1427 and pgm are separated by 107 nucleotides
DVU1425: gcvPA - glycine cleavage system P protein, subunit 1 (TIGR), at 1,499,359 to 1,500,690
gcvPA
DVU1426: gcvH - glycine cleavage system H protein (TIGR), at 1,500,705 to 1,501,088
gcvH
DVU1427: DVU1427 - response regulator (TIGR), at 1,501,280 to 1,501,693
DVU1427
DVU1428: pgm - phosphoglucomutase, alpha-D-glucose phosphate-specific (TIGR), at 1,501,801 to 1,503,456
pgm
Position (kb)
1500
1501
1502 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1499.707 kb on + strand, within gcvPA at 1499.712 kb on + strand, within gcvPA at 1499.778 kb on + strand, within gcvPA at 1499.798 kb on + strand, within gcvPA at 1500.030 kb on + strand, within gcvPA at 1500.078 kb on - strand, within gcvPA at 1500.265 kb on + strand, within gcvPA at 1500.327 kb on - strand, within gcvPA at 1500.345 kb on - strand, within gcvPA at 1500.379 kb on - strand, within gcvPA at 1500.394 kb on - strand, within gcvPA at 1500.394 kb on - strand, within gcvPA at 1500.404 kb on - strand, within gcvPA at 1500.413 kb on + strand, within gcvPA at 1500.413 kb on + strand, within gcvPA at 1500.413 kb on + strand, within gcvPA at 1500.413 kb on + strand, within gcvPA at 1500.415 kb on + strand, within gcvPA at 1500.415 kb on + strand, within gcvPA at 1500.421 kb on - strand, within gcvPA at 1500.421 kb on - strand, within gcvPA at 1500.421 kb on - strand, within gcvPA at 1500.421 kb on - strand, within gcvPA at 1500.449 kb on + strand, within gcvPA at 1500.457 kb on - strand, within gcvPA at 1500.457 kb on - strand, within gcvPA at 1500.493 kb on + strand, within gcvPA at 1500.560 kb on + strand at 1500.560 kb on + strand at 1500.560 kb on + strand at 1500.568 kb on - strand at 1500.568 kb on - strand at 1500.578 kb on + strand at 1500.592 kb on - strand at 1500.651 kb on - strand at 1500.685 kb on + strand at 1500.685 kb on + strand at 1500.693 kb on - strand at 1500.766 kb on - strand, within gcvH at 1500.788 kb on + strand, within gcvH at 1500.797 kb on + strand, within gcvH at 1500.802 kb on + strand, within gcvH at 1500.814 kb on + strand, within gcvH at 1500.918 kb on + strand, within gcvH at 1500.943 kb on - strand, within gcvH at 1500.962 kb on + strand, within gcvH at 1501.091 kb on - strand at 1501.096 kb on + strand at 1501.099 kb on + strand at 1501.102 kb on - strand at 1501.148 kb on - strand at 1501.250 kb on - strand at 1501.254 kb on + strand at 1501.776 kb on - strand at 1501.854 kb on + strand at 1501.998 kb on + strand, within pgm at 1502.006 kb on - strand, within pgm at 1502.006 kb on - strand, within pgm at 1502.045 kb on + strand, within pgm at 1502.053 kb on - strand, within pgm at 1502.053 kb on - strand, within pgm
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with N2 gas (N) remove 1,499,707 + gcvPA DVU1425 0.26 -2.6 1,499,712 + gcvPA DVU1425 0.27 -1.3 1,499,778 + gcvPA DVU1425 0.31 -0.1 1,499,798 + gcvPA DVU1425 0.33 -1.2 1,500,030 + gcvPA DVU1425 0.50 -1.7 1,500,078 - gcvPA DVU1425 0.54 -2.2 1,500,265 + gcvPA DVU1425 0.68 -1.3 1,500,327 - gcvPA DVU1425 0.73 -0.3 1,500,345 - gcvPA DVU1425 0.74 -1.4 1,500,379 - gcvPA DVU1425 0.77 -0.5 1,500,394 - gcvPA DVU1425 0.78 -1.6 1,500,394 - gcvPA DVU1425 0.78 -1.7 1,500,404 - gcvPA DVU1425 0.78 -1.9 1,500,413 + gcvPA DVU1425 0.79 -1.4 1,500,413 + gcvPA DVU1425 0.79 +0.4 1,500,413 + gcvPA DVU1425 0.79 -3.0 1,500,413 + gcvPA DVU1425 0.79 -1.4 1,500,415 + gcvPA DVU1425 0.79 -1.8 1,500,415 + gcvPA DVU1425 0.79 -0.5 1,500,421 - gcvPA DVU1425 0.80 -2.0 1,500,421 - gcvPA DVU1425 0.80 -1.2 1,500,421 - gcvPA DVU1425 0.80 -3.3 1,500,421 - gcvPA DVU1425 0.80 -1.3 1,500,449 + gcvPA DVU1425 0.82 -1.6 1,500,457 - gcvPA DVU1425 0.82 -0.6 1,500,457 - gcvPA DVU1425 0.82 -1.6 1,500,493 + gcvPA DVU1425 0.85 -3.1 1,500,560 + -1.5 1,500,560 + -1.5 1,500,560 + +0.3 1,500,568 - -1.8 1,500,568 - -1.7 1,500,578 + -0.9 1,500,592 - -1.0 1,500,651 - -1.1 1,500,685 + -1.9 1,500,685 + -1.0 1,500,693 - -0.5 1,500,766 - gcvH DVU1426 0.16 -1.5 1,500,788 + gcvH DVU1426 0.22 -2.2 1,500,797 + gcvH DVU1426 0.24 -1.9 1,500,802 + gcvH DVU1426 0.25 -1.6 1,500,814 + gcvH DVU1426 0.28 -0.7 1,500,918 + gcvH DVU1426 0.55 -1.4 1,500,943 - gcvH DVU1426 0.62 -1.7 1,500,962 + gcvH DVU1426 0.67 -2.5 1,501,091 - -1.0 1,501,096 + -0.5 1,501,099 + -1.0 1,501,102 - -0.6 1,501,148 - +0.9 1,501,250 - -1.8 1,501,254 + -0.9 1,501,776 - -0.2 1,501,854 + +0.2 1,501,998 + pgm DVU1428 0.12 +0.0 1,502,006 - pgm DVU1428 0.12 +0.3 1,502,006 - pgm DVU1428 0.12 +0.2 1,502,045 + pgm DVU1428 0.15 -3.3 1,502,053 - pgm DVU1428 0.15 +0.5 1,502,053 - pgm DVU1428 0.15 +0.1
Or see this region's nucleotide sequence