Experiment: MoLS4 with N2 gas (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lipA and lipB overlap by 71 nucleotides lipB and DVU0907 overlap by 14 nucleotides DVU0907 and DVU0908 are separated by 11 nucleotides
DVU0905: lipA - lipoic acid synthetase (TIGR), at 997,479 to 998,375
lipA
DVU0906: lipB - lipoate-protein ligase B (TIGR), at 998,305 to 998,961
lipB
DVU0907: DVU0907 - conserved domain protein (TIGR), at 998,948 to 1,001,887
DVU0907
DVU0908: DVU0908 - ATP-dependent reduction of co(II)balamin (from data) , at 1,001,899 to 1,003,530
DVU0908
Position (kb)
998
999
1000
1001
1002 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 997.968 kb on + strand, within lipA at 997.976 kb on - strand, within lipA at 997.986 kb on - strand, within lipA at 997.986 kb on - strand, within lipA at 998.021 kb on - strand, within lipA at 998.069 kb on + strand, within lipA at 998.252 kb on - strand, within lipA at 998.264 kb on - strand, within lipA at 998.277 kb on - strand, within lipA at 998.277 kb on - strand, within lipA at 998.277 kb on - strand, within lipA at 998.383 kb on - strand, within lipB at 998.554 kb on - strand, within lipB at 998.587 kb on + strand, within lipB at 998.601 kb on + strand, within lipB at 998.859 kb on + strand, within lipB at 998.923 kb on - strand at 998.927 kb on + strand at 999.021 kb on + strand at 999.039 kb on + strand at 999.051 kb on + strand at 999.116 kb on + strand at 999.124 kb on - strand at 999.182 kb on - strand at 1000.172 kb on + strand, within DVU0907 at 1000.295 kb on + strand, within DVU0907 at 1000.766 kb on + strand, within DVU0907 at 1000.827 kb on + strand, within DVU0907 at 1000.827 kb on + strand, within DVU0907 at 1000.827 kb on + strand, within DVU0907 at 1000.827 kb on + strand, within DVU0907 at 1000.835 kb on - strand, within DVU0907 at 1000.835 kb on - strand, within DVU0907 at 1000.835 kb on - strand, within DVU0907 at 1000.874 kb on + strand, within DVU0907 at 1000.881 kb on - strand, within DVU0907 at 1000.881 kb on - strand, within DVU0907 at 1000.936 kb on - strand, within DVU0907 at 1000.993 kb on + strand, within DVU0907 at 1001.008 kb on - strand, within DVU0907 at 1001.139 kb on + strand, within DVU0907 at 1001.142 kb on - strand, within DVU0907 at 1001.201 kb on - strand, within DVU0907 at 1001.269 kb on - strand, within DVU0907 at 1001.269 kb on - strand, within DVU0907 at 1001.269 kb on - strand, within DVU0907 at 1001.326 kb on - strand, within DVU0907 at 1001.397 kb on + strand, within DVU0907 at 1001.407 kb on - strand, within DVU0907 at 1001.458 kb on - strand, within DVU0907 at 1001.493 kb on + strand, within DVU0907 at 1001.506 kb on - strand, within DVU0907 at 1001.547 kb on + strand, within DVU0907 at 1001.547 kb on + strand, within DVU0907 at 1001.557 kb on + strand, within DVU0907 at 1001.605 kb on + strand at 1001.605 kb on + strand at 1001.605 kb on + strand at 1001.610 kb on + strand at 1001.672 kb on - strand at 1001.777 kb on - strand at 1001.793 kb on + strand at 1001.829 kb on + strand at 1001.972 kb on - strand at 1001.997 kb on - strand at 1002.047 kb on + strand at 1002.055 kb on - strand at 1002.087 kb on + strand, within DVU0908 at 1002.102 kb on - strand, within DVU0908 at 1002.109 kb on - strand, within DVU0908 at 1002.183 kb on - strand, within DVU0908 at 1002.319 kb on + strand, within DVU0908 at 1002.319 kb on + strand, within DVU0908 at 1002.326 kb on + strand, within DVU0908 at 1002.327 kb on - strand, within DVU0908 at 1002.529 kb on - strand, within DVU0908 at 1002.561 kb on + strand, within DVU0908 at 1002.562 kb on - strand, within DVU0908 at 1002.593 kb on - strand, within DVU0908 at 1002.610 kb on + strand, within DVU0908 at 1002.620 kb on + strand, within DVU0908 at 1002.670 kb on - strand, within DVU0908 at 1002.680 kb on - strand, within DVU0908 at 1002.680 kb on - strand, within DVU0908 at 1002.701 kb on + strand, within DVU0908 at 1002.709 kb on - strand, within DVU0908 at 1002.709 kb on - strand, within DVU0908 at 1002.720 kb on + strand, within DVU0908 at 1002.762 kb on - strand, within DVU0908 at 1002.807 kb on - strand, within DVU0908
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with N2 gas (N) remove 997,968 + lipA DVU0905 0.55 -2.1 997,976 - lipA DVU0905 0.55 -1.1 997,986 - lipA DVU0905 0.57 -3.9 997,986 - lipA DVU0905 0.57 -0.5 998,021 - lipA DVU0905 0.60 -1.4 998,069 + lipA DVU0905 0.66 -1.6 998,252 - lipA DVU0905 0.86 -0.6 998,264 - lipA DVU0905 0.88 -1.6 998,277 - lipA DVU0905 0.89 -1.4 998,277 - lipA DVU0905 0.89 -0.6 998,277 - lipA DVU0905 0.89 -0.8 998,383 - lipB DVU0906 0.12 -0.8 998,554 - lipB DVU0906 0.38 -1.9 998,587 + lipB DVU0906 0.43 -1.8 998,601 + lipB DVU0906 0.45 -2.0 998,859 + lipB DVU0906 0.84 -0.8 998,923 - -1.1 998,927 + -1.8 999,021 + +0.5 999,039 + -0.2 999,051 + -0.3 999,116 + -0.3 999,124 - +0.1 999,182 - +0.8 1,000,172 + DVU0907 0.42 +0.0 1,000,295 + DVU0907 0.46 +0.6 1,000,766 + DVU0907 0.62 +0.3 1,000,827 + DVU0907 0.64 +1.8 1,000,827 + DVU0907 0.64 +0.1 1,000,827 + DVU0907 0.64 +0.8 1,000,827 + DVU0907 0.64 +0.5 1,000,835 - DVU0907 0.64 -0.4 1,000,835 - DVU0907 0.64 +0.6 1,000,835 - DVU0907 0.64 -0.1 1,000,874 + DVU0907 0.66 -0.2 1,000,881 - DVU0907 0.66 +0.9 1,000,881 - DVU0907 0.66 +0.6 1,000,936 - DVU0907 0.68 +0.5 1,000,993 + DVU0907 0.70 -0.0 1,001,008 - DVU0907 0.70 -0.0 1,001,139 + DVU0907 0.75 -0.0 1,001,142 - DVU0907 0.75 -0.2 1,001,201 - DVU0907 0.77 +0.9 1,001,269 - DVU0907 0.79 +0.4 1,001,269 - DVU0907 0.79 +0.3 1,001,269 - DVU0907 0.79 +0.5 1,001,326 - DVU0907 0.81 +0.4 1,001,397 + DVU0907 0.83 +0.2 1,001,407 - DVU0907 0.84 -2.6 1,001,458 - DVU0907 0.85 +0.2 1,001,493 + DVU0907 0.87 +0.2 1,001,506 - DVU0907 0.87 +1.3 1,001,547 + DVU0907 0.88 +0.0 1,001,547 + DVU0907 0.88 -0.5 1,001,557 + DVU0907 0.89 -0.4 1,001,605 + +1.2 1,001,605 + -0.3 1,001,605 + -0.6 1,001,610 + +0.4 1,001,672 - +0.3 1,001,777 - +0.1 1,001,793 + -0.3 1,001,829 + -0.6 1,001,972 - -2.6 1,001,997 - -3.0 1,002,047 + -2.3 1,002,055 - -3.5 1,002,087 + DVU0908 0.12 -2.9 1,002,102 - DVU0908 0.12 -4.3 1,002,109 - DVU0908 0.13 -3.3 1,002,183 - DVU0908 0.17 -3.6 1,002,319 + DVU0908 0.26 -0.4 1,002,319 + DVU0908 0.26 -4.4 1,002,326 + DVU0908 0.26 -4.8 1,002,327 - DVU0908 0.26 -2.2 1,002,529 - DVU0908 0.39 -1.5 1,002,561 + DVU0908 0.41 -5.0 1,002,562 - DVU0908 0.41 -2.8 1,002,593 - DVU0908 0.43 -2.7 1,002,610 + DVU0908 0.44 -5.1 1,002,620 + DVU0908 0.44 -3.1 1,002,670 - DVU0908 0.47 -3.4 1,002,680 - DVU0908 0.48 -3.2 1,002,680 - DVU0908 0.48 -2.8 1,002,701 + DVU0908 0.49 -3.5 1,002,709 - DVU0908 0.50 -4.2 1,002,709 - DVU0908 0.50 -2.4 1,002,720 + DVU0908 0.50 -4.8 1,002,762 - DVU0908 0.53 -4.5 1,002,807 - DVU0908 0.56 -4.3
Or see this region's nucleotide sequence