Experiment: BG11 with Chlorate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt psaJ and psaF are separated by 25 nucleotides psaF and Synpcc7942_1251 are separated by 177 nucleotides Synpcc7942_1251 and Synpcc7942_1252 are separated by 68 nucleotides
Synpcc7942_1249: psaJ - photosystem I reaction center subunit IX, at 1,273,621 to 1,273,755
psaJ
Synpcc7942_1250: psaF - photosystem I reaction center subunit III precursor, at 1,273,781 to 1,274,260
psaF
Synpcc7942_1251: Synpcc7942_1251 - O-sialoglycoprotein endopeptidase, at 1,274,438 to 1,275,487
_1251
Synpcc7942_1252: Synpcc7942_1252 - DNA repair protein RAD32-like, at 1,275,556 to 1,276,833
_1252
Position (kb)
1274
1275
1276 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1273.469 kb on + strand at 1273.587 kb on - strand at 1273.962 kb on - strand, within psaF at 1274.169 kb on - strand, within psaF at 1274.193 kb on + strand, within psaF at 1274.218 kb on + strand at 1274.271 kb on + strand at 1274.285 kb on + strand at 1274.285 kb on + strand at 1274.293 kb on - strand at 1275.466 kb on + strand at 1275.482 kb on + strand at 1275.488 kb on - strand at 1275.490 kb on + strand at 1275.492 kb on + strand at 1275.499 kb on - strand at 1275.500 kb on - strand at 1275.500 kb on - strand at 1275.500 kb on - strand at 1275.505 kb on - strand at 1275.507 kb on + strand at 1275.524 kb on + strand at 1275.530 kb on - strand at 1275.643 kb on + strand at 1275.683 kb on - strand at 1275.760 kb on + strand, within Synpcc7942_1252 at 1275.761 kb on + strand, within Synpcc7942_1252 at 1275.762 kb on + strand, within Synpcc7942_1252 at 1275.768 kb on - strand, within Synpcc7942_1252 at 1275.781 kb on + strand, within Synpcc7942_1252 at 1275.789 kb on - strand, within Synpcc7942_1252 at 1275.833 kb on - strand, within Synpcc7942_1252 at 1275.833 kb on - strand, within Synpcc7942_1252 at 1275.833 kb on - strand, within Synpcc7942_1252 at 1275.852 kb on + strand, within Synpcc7942_1252 at 1275.929 kb on - strand, within Synpcc7942_1252 at 1275.970 kb on - strand, within Synpcc7942_1252 at 1275.980 kb on - strand, within Synpcc7942_1252 at 1276.070 kb on + strand, within Synpcc7942_1252 at 1276.070 kb on + strand, within Synpcc7942_1252 at 1276.070 kb on + strand, within Synpcc7942_1252 at 1276.070 kb on + strand, within Synpcc7942_1252 at 1276.070 kb on + strand, within Synpcc7942_1252 at 1276.070 kb on + strand, within Synpcc7942_1252 at 1276.078 kb on - strand, within Synpcc7942_1252 at 1276.078 kb on - strand, within Synpcc7942_1252 at 1276.078 kb on - strand, within Synpcc7942_1252 at 1276.078 kb on - strand, within Synpcc7942_1252 at 1276.078 kb on - strand, within Synpcc7942_1252 at 1276.078 kb on - strand, within Synpcc7942_1252 at 1276.124 kb on + strand, within Synpcc7942_1252 at 1276.135 kb on + strand, within Synpcc7942_1252 at 1276.158 kb on - strand, within Synpcc7942_1252 at 1276.169 kb on + strand, within Synpcc7942_1252 at 1276.179 kb on + strand, within Synpcc7942_1252 at 1276.204 kb on - strand, within Synpcc7942_1252 at 1276.273 kb on + strand, within Synpcc7942_1252 at 1276.281 kb on - strand, within Synpcc7942_1252 at 1276.295 kb on - strand, within Synpcc7942_1252 at 1276.299 kb on + strand, within Synpcc7942_1252 at 1276.311 kb on + strand, within Synpcc7942_1252 at 1276.321 kb on + strand, within Synpcc7942_1252 at 1276.321 kb on + strand, within Synpcc7942_1252 at 1276.321 kb on + strand, within Synpcc7942_1252 at 1276.329 kb on - strand, within Synpcc7942_1252 at 1276.367 kb on + strand, within Synpcc7942_1252 at 1276.484 kb on + strand, within Synpcc7942_1252
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with Chlorate 20 mM remove 1,273,469 + -0.9 1,273,587 - +0.6 1,273,962 - psaF Synpcc7942_1250 0.38 +0.4 1,274,169 - psaF Synpcc7942_1250 0.81 -0.6 1,274,193 + psaF Synpcc7942_1250 0.86 -1.4 1,274,218 + -0.6 1,274,271 + -1.1 1,274,285 + -1.4 1,274,285 + +0.9 1,274,293 - +0.1 1,275,466 + +0.9 1,275,482 + -0.1 1,275,488 - +0.4 1,275,490 + -0.1 1,275,492 + +1.5 1,275,499 - -0.4 1,275,500 - -0.4 1,275,500 - -1.3 1,275,500 - +0.4 1,275,505 - -1.1 1,275,507 + +0.1 1,275,524 + +0.7 1,275,530 - +0.9 1,275,643 + -0.6 1,275,683 - -0.1 1,275,760 + Synpcc7942_1252 0.16 -0.9 1,275,761 + Synpcc7942_1252 0.16 -1.6 1,275,762 + Synpcc7942_1252 0.16 -0.4 1,275,768 - Synpcc7942_1252 0.17 -1.6 1,275,781 + Synpcc7942_1252 0.18 -0.6 1,275,789 - Synpcc7942_1252 0.18 -0.9 1,275,833 - Synpcc7942_1252 0.22 -1.6 1,275,833 - Synpcc7942_1252 0.22 -0.8 1,275,833 - Synpcc7942_1252 0.22 -0.6 1,275,852 + Synpcc7942_1252 0.23 -2.1 1,275,929 - Synpcc7942_1252 0.29 -1.0 1,275,970 - Synpcc7942_1252 0.32 -1.2 1,275,980 - Synpcc7942_1252 0.33 +0.6 1,276,070 + Synpcc7942_1252 0.40 -1.8 1,276,070 + Synpcc7942_1252 0.40 -1.9 1,276,070 + Synpcc7942_1252 0.40 -1.4 1,276,070 + Synpcc7942_1252 0.40 -2.3 1,276,070 + Synpcc7942_1252 0.40 -0.4 1,276,070 + Synpcc7942_1252 0.40 -1.6 1,276,078 - Synpcc7942_1252 0.41 -2.3 1,276,078 - Synpcc7942_1252 0.41 -3.0 1,276,078 - Synpcc7942_1252 0.41 -1.3 1,276,078 - Synpcc7942_1252 0.41 +0.1 1,276,078 - Synpcc7942_1252 0.41 -0.6 1,276,078 - Synpcc7942_1252 0.41 -2.6 1,276,124 + Synpcc7942_1252 0.44 -1.4 1,276,135 + Synpcc7942_1252 0.45 -1.1 1,276,158 - Synpcc7942_1252 0.47 -1.5 1,276,169 + Synpcc7942_1252 0.48 +0.4 1,276,179 + Synpcc7942_1252 0.49 -1.7 1,276,204 - Synpcc7942_1252 0.51 -0.4 1,276,273 + Synpcc7942_1252 0.56 -1.8 1,276,281 - Synpcc7942_1252 0.57 -3.0 1,276,295 - Synpcc7942_1252 0.58 -1.9 1,276,299 + Synpcc7942_1252 0.58 -1.1 1,276,311 + Synpcc7942_1252 0.59 -1.9 1,276,321 + Synpcc7942_1252 0.60 -1.8 1,276,321 + Synpcc7942_1252 0.60 -0.9 1,276,321 + Synpcc7942_1252 0.60 -1.2 1,276,329 - Synpcc7942_1252 0.60 -2.3 1,276,367 + Synpcc7942_1252 0.63 -1.4 1,276,484 + Synpcc7942_1252 0.73 -0.4
Or see this region's nucleotide sequence