Experiment: Nitrate reduction, replicate C t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_581 and Pf1N1B4_582 are separated by 157 nucleotides Pf1N1B4_582 and Pf1N1B4_583 are separated by 201 nucleotides Pf1N1B4_583 and Pf1N1B4_584 are separated by 25 nucleotides
Pf1N1B4_581 - membrane protein, putative, at 672,568 to 674,556
_581
Pf1N1B4_582 - FIG00959696: hypothetical protein, at 674,714 to 675,181
_582
Pf1N1B4_583 - FIG00957748: hypothetical protein, at 675,383 to 675,622
_583
Pf1N1B4_584 - Isopenicillin N epimerase (EC 5.1.1.17), at 675,648 to 676,829
_584
Position (kb)
674
675
676 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 673.946 kb on - strand, within Pf1N1B4_581 at 673.946 kb on - strand, within Pf1N1B4_581 at 673.955 kb on - strand, within Pf1N1B4_581 at 673.963 kb on - strand, within Pf1N1B4_581 at 674.161 kb on - strand, within Pf1N1B4_581 at 674.213 kb on + strand, within Pf1N1B4_581 at 674.251 kb on - strand, within Pf1N1B4_581 at 674.410 kb on - strand at 674.558 kb on + strand at 674.559 kb on - strand at 674.559 kb on - strand at 674.559 kb on - strand at 674.559 kb on - strand at 674.559 kb on - strand at 674.634 kb on + strand at 674.656 kb on - strand at 674.715 kb on + strand at 674.715 kb on + strand at 674.715 kb on + strand at 674.715 kb on + strand at 674.715 kb on + strand at 674.716 kb on - strand at 674.716 kb on - strand at 674.716 kb on - strand at 674.716 kb on - strand at 674.716 kb on - strand at 674.716 kb on - strand at 674.716 kb on - strand at 674.716 kb on - strand at 674.716 kb on - strand at 674.781 kb on + strand, within Pf1N1B4_582 at 674.850 kb on + strand, within Pf1N1B4_582 at 674.851 kb on - strand, within Pf1N1B4_582 at 674.851 kb on - strand, within Pf1N1B4_582 at 674.851 kb on - strand, within Pf1N1B4_582 at 674.851 kb on - strand, within Pf1N1B4_582 at 674.851 kb on - strand, within Pf1N1B4_582 at 674.851 kb on - strand, within Pf1N1B4_582 at 675.056 kb on - strand, within Pf1N1B4_582 at 675.056 kb on - strand, within Pf1N1B4_582 at 675.056 kb on - strand, within Pf1N1B4_582 at 675.149 kb on + strand at 675.150 kb on - strand at 675.150 kb on - strand at 675.166 kb on + strand at 675.167 kb on - strand at 675.167 kb on - strand at 675.169 kb on + strand at 675.215 kb on + strand at 675.215 kb on + strand at 675.215 kb on + strand at 675.216 kb on - strand at 675.216 kb on - strand at 675.220 kb on - strand at 675.441 kb on - strand, within Pf1N1B4_583 at 675.441 kb on - strand, within Pf1N1B4_583 at 675.441 kb on - strand, within Pf1N1B4_583 at 675.441 kb on - strand, within Pf1N1B4_583 at 675.784 kb on - strand, within Pf1N1B4_584 at 675.960 kb on + strand, within Pf1N1B4_584 at 675.960 kb on + strand, within Pf1N1B4_584 at 675.961 kb on - strand, within Pf1N1B4_584 at 675.961 kb on - strand, within Pf1N1B4_584 at 675.961 kb on - strand, within Pf1N1B4_584 at 675.961 kb on - strand, within Pf1N1B4_584 at 675.961 kb on - strand at 675.995 kb on - strand at 676.008 kb on + strand, within Pf1N1B4_584
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t1 remove 673,946 - Pf1N1B4_581 0.69 -0.0 673,946 - Pf1N1B4_581 0.69 -2.9 673,955 - Pf1N1B4_581 0.70 -0.0 673,963 - Pf1N1B4_581 0.70 -2.0 674,161 - Pf1N1B4_581 0.80 -3.0 674,213 + Pf1N1B4_581 0.83 -3.5 674,251 - Pf1N1B4_581 0.85 -2.8 674,410 - -0.0 674,558 + -0.0 674,559 - -0.0 674,559 - -2.9 674,559 - -3.2 674,559 - -0.0 674,559 - -0.0 674,634 + -1.8 674,656 - -0.0 674,715 + -0.0 674,715 + -0.0 674,715 + -0.0 674,715 + -0.0 674,715 + -0.0 674,716 - -0.0 674,716 - -0.0 674,716 - -0.0 674,716 - -0.0 674,716 - -0.0 674,716 - -1.6 674,716 - -0.0 674,716 - -2.4 674,716 - -0.0 674,781 + Pf1N1B4_582 0.14 -0.0 674,850 + Pf1N1B4_582 0.29 -3.7 674,851 - Pf1N1B4_582 0.29 -3.2 674,851 - Pf1N1B4_582 0.29 -0.0 674,851 - Pf1N1B4_582 0.29 -0.0 674,851 - Pf1N1B4_582 0.29 -0.0 674,851 - Pf1N1B4_582 0.29 -2.6 674,851 - Pf1N1B4_582 0.29 -2.7 675,056 - Pf1N1B4_582 0.73 -0.0 675,056 - Pf1N1B4_582 0.73 -0.0 675,056 - Pf1N1B4_582 0.73 -4.2 675,149 + -0.0 675,150 - -0.0 675,150 - -0.0 675,166 + -2.1 675,167 - -0.0 675,167 - -0.0 675,169 + -0.0 675,215 + -0.0 675,215 + -1.4 675,215 + -1.7 675,216 - -0.0 675,216 - -1.5 675,220 - -2.7 675,441 - Pf1N1B4_583 0.24 -0.0 675,441 - Pf1N1B4_583 0.24 -2.6 675,441 - Pf1N1B4_583 0.24 -0.0 675,441 - Pf1N1B4_583 0.24 -1.6 675,784 - Pf1N1B4_584 0.12 -3.0 675,960 + Pf1N1B4_584 0.26 -0.7 675,960 + Pf1N1B4_584 0.26 -0.0 675,961 - Pf1N1B4_584 0.26 -0.0 675,961 - Pf1N1B4_584 0.26 -0.0 675,961 - Pf1N1B4_584 0.26 -0.0 675,961 - Pf1N1B4_584 0.26 -0.0 675,961 - -0.8 675,995 - -3.4 676,008 + Pf1N1B4_584 0.30 -0.0
Or see this region's nucleotide sequence