Experiment: Nitrate reduction, replicate C t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5770 and Pf1N1B4_5771 overlap by 26 nucleotides Pf1N1B4_5771 and Pf1N1B4_5772 are separated by 198 nucleotides Pf1N1B4_5772 and Pf1N1B4_5773 are separated by 70 nucleotides Pf1N1B4_5773 and Pf1N1B4_5774 overlap by 4 nucleotides
Pf1N1B4_5770 - Heme d1 biosynthesis protein NirG, at 207,646 to 208,089
_5770
Pf1N1B4_5771 - Heme d1 biosynthesis protein NirH, at 208,064 to 208,579
_5771
Pf1N1B4_5772 - Heme d1 biosynthesis protein NirJ, at 208,778 to 209,956
_5772
Pf1N1B4_5773 - Uroporphyrinogen-III methyltransferase (EC 2.1.1.107), at 210,027 to 210,866
_5773
Pf1N1B4_5774 - Nitrite reductase associated c-type cytochorome NirN, at 210,863 to 212,356
_5774
Position (kb) 208
209
210 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 207.929 kb on - strand, within Pf1N1B4_5770 at 207.929 kb on - strand, within Pf1N1B4_5770 at 208.060 kb on + strand at 208.060 kb on + strand at 208.061 kb on - strand at 208.076 kb on - strand at 208.284 kb on - strand, within Pf1N1B4_5771 at 208.548 kb on + strand at 208.618 kb on + strand at 208.665 kb on - strand at 208.732 kb on + strand at 208.957 kb on + strand, within Pf1N1B4_5772 at 208.958 kb on - strand, within Pf1N1B4_5772 at 209.166 kb on - strand, within Pf1N1B4_5772 at 209.373 kb on - strand, within Pf1N1B4_5772 at 209.373 kb on - strand, within Pf1N1B4_5772 at 209.391 kb on - strand, within Pf1N1B4_5772 at 209.681 kb on + strand, within Pf1N1B4_5772 at 209.681 kb on + strand, within Pf1N1B4_5772 at 209.681 kb on + strand, within Pf1N1B4_5772 at 210.378 kb on + strand, within Pf1N1B4_5773 at 210.579 kb on + strand, within Pf1N1B4_5773 at 210.580 kb on - strand, within Pf1N1B4_5773
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t1 remove 207,929 - Pf1N1B4_5770 0.64 +0.0 207,929 - Pf1N1B4_5770 0.64 +0.0 208,060 + +0.3 208,060 + -1.3 208,061 - +0.4 208,076 - +0.0 208,284 - Pf1N1B4_5771 0.43 +0.0 208,548 + -2.1 208,618 + +0.0 208,665 - -1.3 208,732 + -1.4 208,957 + Pf1N1B4_5772 0.15 -1.0 208,958 - Pf1N1B4_5772 0.15 +0.0 209,166 - Pf1N1B4_5772 0.33 +0.0 209,373 - Pf1N1B4_5772 0.50 +0.3 209,373 - Pf1N1B4_5772 0.50 +0.0 209,391 - Pf1N1B4_5772 0.52 +0.0 209,681 + Pf1N1B4_5772 0.77 +0.0 209,681 + Pf1N1B4_5772 0.77 +0.6 209,681 + Pf1N1B4_5772 0.77 +1.1 210,378 + Pf1N1B4_5773 0.42 +0.0 210,579 + Pf1N1B4_5773 0.66 -4.7 210,580 - Pf1N1B4_5773 0.66 +0.0
Or see this region's nucleotide sequence