Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5767

Experiment: Nitrate reduction, replicate C t1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5764 and Pf1N1B4_5765 are separated by 24 nucleotidesPf1N1B4_5765 and Pf1N1B4_5766 are separated by 16 nucleotidesPf1N1B4_5766 and Pf1N1B4_5767 overlap by 4 nucleotidesPf1N1B4_5767 and Pf1N1B4_5768 are separated by 1 nucleotidesPf1N1B4_5768 and Pf1N1B4_5769 overlap by 4 nucleotidesPf1N1B4_5769 and Pf1N1B4_5770 overlap by 8 nucleotides Pf1N1B4_5764 - Cytochrome cd1 nitrite reductase (EC:1.7.2.1), at 203,133 to 204,824 _5764 Pf1N1B4_5765 - membrane c-type cytochrome cy, at 204,849 to 205,163 _5765 Pf1N1B4_5766 - Cytochrome c55X precursor NirC, at 205,180 to 205,506 _5766 Pf1N1B4_5767 - Heme d1 biosynthesis protein NirF, at 205,503 to 206,681 _5767 Pf1N1B4_5768 - Heme d1 biosynthesis protein NirD, at 206,683 to 207,141 _5768 Pf1N1B4_5769 - Heme d1 biosynthesis protein NirL, at 207,138 to 207,653 _5769 Pf1N1B4_5770 - Heme d1 biosynthesis protein NirG, at 207,646 to 208,089 _5770 Position (kb) 205 206 207Strain fitness (log2 ratio) -2 -1 0 1at 204.648 kb on + strand, within Pf1N1B4_5764at 204.648 kb on + strand, within Pf1N1B4_5764at 204.648 kb on + strand, within Pf1N1B4_5764at 204.754 kb on + strandat 204.755 kb on - strandat 204.819 kb on + strandat 204.819 kb on + strandat 204.819 kb on + strandat 204.819 kb on + strandat 204.820 kb on - strandat 204.820 kb on - strandat 204.820 kb on - strandat 204.820 kb on - strandat 204.857 kb on + strandat 205.201 kb on - strandat 205.526 kb on - strandat 205.725 kb on + strand, within Pf1N1B4_5767at 205.725 kb on + strand, within Pf1N1B4_5767at 205.725 kb on + strand, within Pf1N1B4_5767at 205.725 kb on + strandat 205.726 kb on - strand, within Pf1N1B4_5767at 205.726 kb on - strand, within Pf1N1B4_5767at 205.726 kb on - strand, within Pf1N1B4_5767at 205.726 kb on - strand, within Pf1N1B4_5767at 205.726 kb on - strand, within Pf1N1B4_5767at 205.726 kb on - strand, within Pf1N1B4_5767at 205.796 kb on - strand, within Pf1N1B4_5767at 205.860 kb on + strand, within Pf1N1B4_5767at 205.861 kb on - strand, within Pf1N1B4_5767at 205.861 kb on - strand, within Pf1N1B4_5767at 205.861 kb on - strand, within Pf1N1B4_5767at 205.861 kb on - strandat 205.861 kb on - strand, within Pf1N1B4_5767at 206.094 kb on + strand, within Pf1N1B4_5767at 206.095 kb on - strand, within Pf1N1B4_5767at 206.127 kb on + strand, within Pf1N1B4_5767at 206.127 kb on + strand, within Pf1N1B4_5767at 206.127 kb on + strand, within Pf1N1B4_5767at 206.127 kb on + strand, within Pf1N1B4_5767at 206.128 kb on - strand, within Pf1N1B4_5767at 206.756 kb on - strand, within Pf1N1B4_5768at 206.756 kb on - strand, within Pf1N1B4_5768at 206.964 kb on + strand, within Pf1N1B4_5768at 206.965 kb on - strand, within Pf1N1B4_5768at 206.968 kb on + strandat 206.968 kb on + strand, within Pf1N1B4_5768at 206.969 kb on - strand, within Pf1N1B4_5768at 206.987 kb on - strand, within Pf1N1B4_5768at 207.344 kb on + strand, within Pf1N1B4_5769at 207.344 kb on + strand, within Pf1N1B4_5769at 207.439 kb on - strand, within Pf1N1B4_5769at 207.472 kb on - strand, within Pf1N1B4_5769

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t1
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204,648 + Pf1N1B4_5764 0.90 +0.0
204,648 + Pf1N1B4_5764 0.90 +0.0
204,648 + Pf1N1B4_5764 0.90 +0.3
204,754 + +0.0
204,755 - +0.1
204,819 + +0.0
204,819 + +0.0
204,819 + -0.9
204,819 + +0.0
204,820 - +0.0
204,820 - +0.0
204,820 - +1.6
204,820 - +0.0
204,857 + -1.0
205,201 - +0.0
205,526 - +0.0
205,725 + Pf1N1B4_5767 0.19 +0.6
205,725 + Pf1N1B4_5767 0.19 +0.0
205,725 + Pf1N1B4_5767 0.19 +0.0
205,725 + +0.0
205,726 - Pf1N1B4_5767 0.19 +1.6
205,726 - Pf1N1B4_5767 0.19 +0.0
205,726 - Pf1N1B4_5767 0.19 +0.0
205,726 - Pf1N1B4_5767 0.19 +0.0
205,726 - Pf1N1B4_5767 0.19 +0.0
205,726 - Pf1N1B4_5767 0.19 +0.5
205,796 - Pf1N1B4_5767 0.25 +0.0
205,860 + Pf1N1B4_5767 0.30 +0.4
205,861 - Pf1N1B4_5767 0.30 +0.0
205,861 - Pf1N1B4_5767 0.30 +0.0
205,861 - Pf1N1B4_5767 0.30 +0.0
205,861 - +0.0
205,861 - Pf1N1B4_5767 0.30 +0.9
206,094 + Pf1N1B4_5767 0.50 +0.0
206,095 - Pf1N1B4_5767 0.50 +1.4
206,127 + Pf1N1B4_5767 0.53 +0.0
206,127 + Pf1N1B4_5767 0.53 +0.0
206,127 + Pf1N1B4_5767 0.53 +0.3
206,127 + Pf1N1B4_5767 0.53 +0.5
206,128 - Pf1N1B4_5767 0.53 -2.0
206,756 - Pf1N1B4_5768 0.16 +0.0
206,756 - Pf1N1B4_5768 0.16 +0.0
206,964 + Pf1N1B4_5768 0.61 +0.0
206,965 - Pf1N1B4_5768 0.61 +0.0
206,968 + +0.0
206,968 + Pf1N1B4_5768 0.62 +0.0
206,969 - Pf1N1B4_5768 0.62 +0.8
206,987 - Pf1N1B4_5768 0.66 +0.4
207,344 + Pf1N1B4_5769 0.40 +0.0
207,344 + Pf1N1B4_5769 0.40 +0.0
207,439 - Pf1N1B4_5769 0.58 +0.0
207,472 - Pf1N1B4_5769 0.65 +0.0

Or see this region's nucleotide sequence