Experiment: Nitrate reduction, replicate C t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5756 and Pf1N1B4_5757 overlap by 4 nucleotides Pf1N1B4_5757 and Pf1N1B4_5758 are separated by 195 nucleotides Pf1N1B4_5758 and Pf1N1B4_5759 are separated by 78 nucleotides
Pf1N1B4_5756 - Nitric oxide -responding transcriptional regulator Dnr (Crp/Fnr family), at 196,193 to 196,876
_5756
Pf1N1B4_5757 - hypothetical protein, at 196,873 to 197,067
_5757
Pf1N1B4_5758 - Nitric oxide reductase activation protein NorD, at 197,263 to 199,104
_5758
Pf1N1B4_5759 - Nitric-oxide reductase subunit B (EC 1.7.99.7), at 199,183 to 200,610
_5759
Position (kb) 197
198
199
200 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 196.352 kb on + strand, within Pf1N1B4_5756 at 196.353 kb on - strand, within Pf1N1B4_5756 at 196.479 kb on - strand, within Pf1N1B4_5756 at 196.538 kb on + strand, within Pf1N1B4_5756 at 196.539 kb on - strand, within Pf1N1B4_5756 at 196.689 kb on + strand, within Pf1N1B4_5756 at 196.897 kb on + strand, within Pf1N1B4_5757 at 196.897 kb on + strand, within Pf1N1B4_5757 at 197.078 kb on + strand at 197.078 kb on + strand at 197.079 kb on - strand at 197.079 kb on - strand at 197.237 kb on + strand at 197.308 kb on + strand at 197.309 kb on - strand at 197.357 kb on + strand at 197.357 kb on + strand at 197.358 kb on - strand at 197.366 kb on + strand at 197.394 kb on + strand at 197.471 kb on + strand, within Pf1N1B4_5758 at 197.471 kb on + strand, within Pf1N1B4_5758 at 197.472 kb on - strand, within Pf1N1B4_5758 at 197.546 kb on - strand, within Pf1N1B4_5758 at 197.546 kb on - strand, within Pf1N1B4_5758 at 197.585 kb on + strand, within Pf1N1B4_5758 at 197.586 kb on - strand, within Pf1N1B4_5758 at 197.586 kb on - strand, within Pf1N1B4_5758 at 197.586 kb on - strand, within Pf1N1B4_5758 at 197.589 kb on - strand, within Pf1N1B4_5758 at 197.764 kb on + strand, within Pf1N1B4_5758 at 197.764 kb on + strand, within Pf1N1B4_5758 at 197.764 kb on + strand, within Pf1N1B4_5758 at 197.765 kb on - strand, within Pf1N1B4_5758 at 198.369 kb on + strand at 198.837 kb on + strand, within Pf1N1B4_5758 at 199.167 kb on + strand at 199.193 kb on - strand at 199.243 kb on - strand at 199.251 kb on - strand at 199.515 kb on - strand, within Pf1N1B4_5759 at 199.553 kb on + strand at 199.554 kb on - strand at 199.554 kb on - strand at 199.669 kb on - strand, within Pf1N1B4_5759 at 199.887 kb on - strand, within Pf1N1B4_5759 at 199.887 kb on - strand, within Pf1N1B4_5759 at 199.887 kb on - strand, within Pf1N1B4_5759 at 200.010 kb on - strand, within Pf1N1B4_5759 at 200.043 kb on - strand, within Pf1N1B4_5759
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t1 remove 196,352 + Pf1N1B4_5756 0.23 -3.2 196,353 - Pf1N1B4_5756 0.23 -4.6 196,479 - Pf1N1B4_5756 0.42 -3.4 196,538 + Pf1N1B4_5756 0.50 +0.0 196,539 - Pf1N1B4_5756 0.51 +0.0 196,689 + Pf1N1B4_5756 0.73 +0.0 196,897 + Pf1N1B4_5757 0.12 -2.8 196,897 + Pf1N1B4_5757 0.12 -3.4 197,078 + -2.9 197,078 + -2.4 197,079 - +0.0 197,079 - +0.0 197,237 + +0.0 197,308 + +0.0 197,309 - -3.6 197,357 + +0.0 197,357 + -3.8 197,358 - -4.0 197,366 + -3.2 197,394 + -3.7 197,471 + Pf1N1B4_5758 0.11 -3.8 197,471 + Pf1N1B4_5758 0.11 -3.3 197,472 - Pf1N1B4_5758 0.11 +0.0 197,546 - Pf1N1B4_5758 0.15 +0.0 197,546 - Pf1N1B4_5758 0.15 -5.3 197,585 + Pf1N1B4_5758 0.17 -3.6 197,586 - Pf1N1B4_5758 0.18 +0.0 197,586 - Pf1N1B4_5758 0.18 -2.2 197,586 - Pf1N1B4_5758 0.18 -4.2 197,589 - Pf1N1B4_5758 0.18 -3.9 197,764 + Pf1N1B4_5758 0.27 -1.6 197,764 + Pf1N1B4_5758 0.27 +0.0 197,764 + Pf1N1B4_5758 0.27 -3.1 197,765 - Pf1N1B4_5758 0.27 -3.7 198,369 + -3.6 198,837 + Pf1N1B4_5758 0.85 -3.3 199,167 + +0.0 199,193 - -2.7 199,243 - -2.9 199,251 - +0.0 199,515 - Pf1N1B4_5759 0.23 +0.0 199,553 + +0.0 199,554 - +0.0 199,554 - +0.0 199,669 - Pf1N1B4_5759 0.34 -1.7 199,887 - Pf1N1B4_5759 0.49 +0.0 199,887 - Pf1N1B4_5759 0.49 -4.3 199,887 - Pf1N1B4_5759 0.49 -3.9 200,010 - Pf1N1B4_5759 0.58 +0.0 200,043 - Pf1N1B4_5759 0.60 +0.0
Or see this region's nucleotide sequence