Experiment: Nitrate reduction, replicate C t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5738 and Pf1N1B4_5739 overlap by 24 nucleotides Pf1N1B4_5739 and Pf1N1B4_5740 are separated by 135 nucleotides Pf1N1B4_5740 and Pf1N1B4_5741 overlap by 4 nucleotides Pf1N1B4_5741 and Pf1N1B4_5742 are separated by 86 nucleotides
Pf1N1B4_5738 - Nitrate/nitrite transporter, at 180,523 to 181,347
_5738
Pf1N1B4_5739 - hypothetical protein, at 181,324 to 181,515
_5739
Pf1N1B4_5740 - Nitrate/nitrite sensor protein (EC 2.7.3.-), at 181,651 to 183,453
_5740
Pf1N1B4_5741 - Nitrate/nitrite response regulator protein, at 183,450 to 184,097
_5741
Pf1N1B4_5742 - Nitric oxide -responding transcriptional regulator Dnr (Crp/Fnr family), at 184,184 to 184,858
_5742
Position (kb) 181
182
183
184 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 180.651 kb on - strand, within Pf1N1B4_5738 at 180.651 kb on - strand at 180.651 kb on - strand, within Pf1N1B4_5738 at 180.704 kb on + strand, within Pf1N1B4_5738 at 180.705 kb on - strand, within Pf1N1B4_5738 at 180.986 kb on + strand, within Pf1N1B4_5738 at 180.986 kb on + strand, within Pf1N1B4_5738 at 180.987 kb on - strand, within Pf1N1B4_5738 at 180.987 kb on - strand, within Pf1N1B4_5738 at 180.987 kb on - strand, within Pf1N1B4_5738 at 181.331 kb on - strand at 181.331 kb on - strand at 181.331 kb on - strand at 181.382 kb on + strand, within Pf1N1B4_5739 at 181.444 kb on - strand, within Pf1N1B4_5739 at 181.564 kb on - strand at 181.564 kb on - strand at 181.958 kb on - strand, within Pf1N1B4_5740 at 181.958 kb on - strand, within Pf1N1B4_5740 at 181.958 kb on - strand, within Pf1N1B4_5740 at 181.992 kb on - strand, within Pf1N1B4_5740 at 182.181 kb on - strand, within Pf1N1B4_5740 at 182.185 kb on + strand, within Pf1N1B4_5740 at 182.810 kb on + strand, within Pf1N1B4_5740 at 182.876 kb on - strand at 182.899 kb on - strand, within Pf1N1B4_5740 at 182.984 kb on - strand, within Pf1N1B4_5740 at 183.222 kb on - strand, within Pf1N1B4_5740 at 183.805 kb on - strand, within Pf1N1B4_5741 at 184.134 kb on + strand at 184.134 kb on + strand at 184.134 kb on + strand at 184.160 kb on + strand at 184.160 kb on + strand at 184.161 kb on - strand at 184.179 kb on - strand at 184.210 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t1 remove 180,651 - Pf1N1B4_5738 0.16 -3.8 180,651 - -2.2 180,651 - Pf1N1B4_5738 0.16 +0.0 180,704 + Pf1N1B4_5738 0.22 -4.9 180,705 - Pf1N1B4_5738 0.22 +0.0 180,986 + Pf1N1B4_5738 0.56 -4.7 180,986 + Pf1N1B4_5738 0.56 +0.0 180,987 - Pf1N1B4_5738 0.56 -3.3 180,987 - Pf1N1B4_5738 0.56 +0.0 180,987 - Pf1N1B4_5738 0.56 +0.0 181,331 - +0.0 181,331 - -2.6 181,331 - -3.9 181,382 + Pf1N1B4_5739 0.30 +0.0 181,444 - Pf1N1B4_5739 0.62 -4.2 181,564 - -2.9 181,564 - +0.0 181,958 - Pf1N1B4_5740 0.17 +0.0 181,958 - Pf1N1B4_5740 0.17 +0.0 181,958 - Pf1N1B4_5740 0.17 -4.0 181,992 - Pf1N1B4_5740 0.19 -3.8 182,181 - Pf1N1B4_5740 0.29 +0.0 182,185 + Pf1N1B4_5740 0.30 +0.0 182,810 + Pf1N1B4_5740 0.64 -1.8 182,876 - +0.0 182,899 - Pf1N1B4_5740 0.69 +0.0 182,984 - Pf1N1B4_5740 0.74 +0.0 183,222 - Pf1N1B4_5740 0.87 -3.1 183,805 - Pf1N1B4_5741 0.55 -3.6 184,134 + +0.0 184,134 + +0.0 184,134 + +0.0 184,160 + +0.0 184,160 + +0.0 184,161 - -2.2 184,179 - +0.0 184,210 - +0.0
Or see this region's nucleotide sequence