Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_564

Experiment: Nitrate reduction, replicate C t1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_562 and Pf1N1B4_563 overlap by 4 nucleotidesPf1N1B4_563 and Pf1N1B4_564 are separated by 233 nucleotidesPf1N1B4_564 and Pf1N1B4_565 are separated by 165 nucleotides Pf1N1B4_562 - Histidine transporter, periplasmic histidine-binding protein, at 658,797 to 659,810 _562 Pf1N1B4_563 - RNA polymerase sigma-70 factor, at 659,807 to 660,355 _563 Pf1N1B4_564 - ABC-type phosphate/phosphonate transport system, periplasmic component, at 660,589 to 661,353 _564 Pf1N1B4_565 - Fatty acid desaturase, at 661,519 to 662,460 _565 Position (kb) 660 661 662Strain fitness (log2 ratio) -3 -2 -1 0 1at 659.892 kb on + strand, within Pf1N1B4_563at 659.892 kb on + strand, within Pf1N1B4_563at 659.892 kb on + strand, within Pf1N1B4_563at 659.892 kb on + strand, within Pf1N1B4_563at 659.893 kb on - strand, within Pf1N1B4_563at 659.893 kb on - strand, within Pf1N1B4_563at 659.943 kb on + strand, within Pf1N1B4_563at 659.943 kb on + strand, within Pf1N1B4_563at 660.138 kb on + strand, within Pf1N1B4_563at 660.355 kb on + strandat 660.355 kb on + strandat 660.355 kb on + strandat 660.355 kb on + strandat 660.355 kb on + strandat 660.356 kb on - strandat 660.356 kb on - strandat 660.412 kb on - strandat 660.412 kb on - strandat 660.412 kb on - strandat 660.412 kb on - strandat 660.485 kb on + strandat 660.485 kb on + strandat 660.485 kb on + strandat 660.485 kb on + strandat 660.485 kb on + strandat 660.485 kb on + strandat 660.485 kb on + strandat 660.485 kb on + strandat 660.485 kb on + strandat 660.486 kb on - strandat 660.486 kb on - strandat 660.651 kb on - strandat 660.785 kb on + strand, within Pf1N1B4_564at 660.785 kb on + strand, within Pf1N1B4_564at 660.786 kb on - strand, within Pf1N1B4_564at 660.863 kb on + strand, within Pf1N1B4_564at 661.102 kb on + strand, within Pf1N1B4_564at 661.102 kb on + strand, within Pf1N1B4_564at 661.139 kb on - strand, within Pf1N1B4_564at 661.349 kb on + strandat 661.349 kb on + strandat 661.349 kb on + strandat 661.350 kb on - strandat 661.350 kb on - strandat 661.350 kb on - strandat 661.350 kb on - strandat 661.350 kb on - strandat 661.357 kb on + strandat 661.357 kb on + strandat 661.357 kb on + strandat 661.357 kb on + strandat 661.358 kb on - strandat 661.358 kb on - strandat 661.358 kb on - strandat 661.392 kb on - strandat 661.403 kb on - strandat 661.403 kb on - strandat 661.403 kb on - strandat 661.403 kb on - strandat 661.403 kb on - strandat 661.469 kb on + strandat 661.469 kb on + strandat 661.469 kb on + strandat 661.470 kb on - strandat 661.524 kb on + strandat 661.524 kb on + strandat 661.524 kb on + strandat 661.524 kb on + strandat 661.525 kb on - strandat 661.525 kb on - strandat 661.583 kb on + strandat 661.584 kb on - strandat 661.584 kb on - strandat 661.584 kb on - strandat 661.584 kb on - strandat 661.641 kb on - strand, within Pf1N1B4_565at 661.659 kb on - strand, within Pf1N1B4_565at 661.659 kb on - strand, within Pf1N1B4_565at 661.787 kb on + strand, within Pf1N1B4_565at 661.787 kb on + strand, within Pf1N1B4_565at 661.788 kb on - strand, within Pf1N1B4_565at 661.788 kb on - strandat 661.788 kb on - strand, within Pf1N1B4_565at 661.788 kb on - strand, within Pf1N1B4_565at 661.788 kb on - strand, within Pf1N1B4_565at 661.788 kb on - strand, within Pf1N1B4_565at 661.790 kb on + strand, within Pf1N1B4_565at 661.790 kb on + strand, within Pf1N1B4_565at 661.790 kb on + strand, within Pf1N1B4_565at 661.790 kb on + strand, within Pf1N1B4_565at 661.791 kb on - strand, within Pf1N1B4_565at 661.791 kb on - strand, within Pf1N1B4_565at 661.791 kb on - strand, within Pf1N1B4_565at 661.791 kb on - strand, within Pf1N1B4_565at 661.791 kb on - strand, within Pf1N1B4_565at 661.791 kb on - strand, within Pf1N1B4_565at 661.791 kb on - strand, within Pf1N1B4_565at 661.841 kb on + strand, within Pf1N1B4_565at 661.911 kb on + strand, within Pf1N1B4_565at 662.088 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t1
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659,892 + Pf1N1B4_563 0.15 -0.0
659,892 + Pf1N1B4_563 0.15 -0.0
659,892 + Pf1N1B4_563 0.15 -2.4
659,892 + Pf1N1B4_563 0.15 -3.1
659,893 - Pf1N1B4_563 0.16 -0.0
659,893 - Pf1N1B4_563 0.16 -2.8
659,943 + Pf1N1B4_563 0.25 -0.0
659,943 + Pf1N1B4_563 0.25 -3.5
660,138 + Pf1N1B4_563 0.60 -0.0
660,355 + -2.3
660,355 + -1.9
660,355 + -0.0
660,355 + -2.2
660,355 + -2.8
660,356 - -0.0
660,356 - -0.0
660,412 - -0.0
660,412 - -1.4
660,412 - -0.0
660,412 - -0.0
660,485 + -2.7
660,485 + -0.0
660,485 + -3.0
660,485 + -0.0
660,485 + -0.0
660,485 + -0.0
660,485 + -0.0
660,485 + -1.0
660,485 + -1.6
660,486 - -0.0
660,486 - -0.0
660,651 - -0.0
660,785 + Pf1N1B4_564 0.26 -3.4
660,785 + Pf1N1B4_564 0.26 -0.0
660,786 - Pf1N1B4_564 0.26 -0.0
660,863 + Pf1N1B4_564 0.36 -0.0
661,102 + Pf1N1B4_564 0.67 -3.0
661,102 + Pf1N1B4_564 0.67 -3.2
661,139 - Pf1N1B4_564 0.72 -2.6
661,349 + -2.8
661,349 + -0.0
661,349 + -0.0
661,350 - -0.0
661,350 - -1.3
661,350 - -3.5
661,350 - -1.5
661,350 - -0.0
661,357 + -0.0
661,357 + -0.0
661,357 + -0.0
661,357 + -2.7
661,358 - -2.5
661,358 - -0.0
661,358 - -0.0
661,392 - -0.0
661,403 - -0.0
661,403 - -0.0
661,403 - -2.8
661,403 - -1.5
661,403 - -1.7
661,469 + -0.0
661,469 + -2.3
661,469 + -0.0
661,470 - -1.7
661,524 + -2.0
661,524 + -0.0
661,524 + -0.0
661,524 + -2.9
661,525 - -2.3
661,525 - -0.0
661,583 + -3.1
661,584 - -2.9
661,584 - -2.7
661,584 - -2.7
661,584 - -0.0
661,641 - Pf1N1B4_565 0.13 -1.3
661,659 - Pf1N1B4_565 0.15 -0.0
661,659 - Pf1N1B4_565 0.15 -0.0
661,787 + Pf1N1B4_565 0.28 -0.0
661,787 + Pf1N1B4_565 0.28 -0.0
661,788 - Pf1N1B4_565 0.29 -2.0
661,788 - -3.0
661,788 - Pf1N1B4_565 0.29 -0.0
661,788 - Pf1N1B4_565 0.29 -0.0
661,788 - Pf1N1B4_565 0.29 -2.6
661,788 - Pf1N1B4_565 0.29 -0.0
661,790 + Pf1N1B4_565 0.29 -0.0
661,790 + Pf1N1B4_565 0.29 -0.0
661,790 + Pf1N1B4_565 0.29 -0.0
661,790 + Pf1N1B4_565 0.29 -3.2
661,791 - Pf1N1B4_565 0.29 -0.0
661,791 - Pf1N1B4_565 0.29 -0.0
661,791 - Pf1N1B4_565 0.29 -0.0
661,791 - Pf1N1B4_565 0.29 -2.2
661,791 - Pf1N1B4_565 0.29 -2.6
661,791 - Pf1N1B4_565 0.29 -0.0
661,791 - Pf1N1B4_565 0.29 -2.1
661,841 + Pf1N1B4_565 0.34 -1.0
661,911 + Pf1N1B4_565 0.42 -0.0
662,088 - -0.0

Or see this region's nucleotide sequence