Experiment: Nitrate reduction, replicate C t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5521 and Pf1N1B4_5522 are separated by 9 nucleotides Pf1N1B4_5522 and Pf1N1B4_5523 are separated by 96 nucleotides
Pf1N1B4_5521 - General secretion pathway protein D / Type II secretion outermembrane pore forming protein (PulD), at 278,116 to 279,762
_5521
Pf1N1B4_5522 - hypothetical protein, at 279,772 to 280,713
_5522
Pf1N1B4_5523 - hypothetical protein, at 280,810 to 282,381
_5523
Position (kb)
279
280
281 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 278.794 kb on + strand, within Pf1N1B4_5521 at 278.794 kb on + strand, within Pf1N1B4_5521 at 278.849 kb on + strand, within Pf1N1B4_5521 at 278.850 kb on - strand, within Pf1N1B4_5521 at 278.850 kb on - strand, within Pf1N1B4_5521 at 278.862 kb on + strand, within Pf1N1B4_5521 at 278.862 kb on + strand, within Pf1N1B4_5521 at 278.907 kb on - strand, within Pf1N1B4_5521 at 278.917 kb on + strand, within Pf1N1B4_5521 at 278.981 kb on + strand at 278.981 kb on + strand, within Pf1N1B4_5521 at 278.981 kb on + strand, within Pf1N1B4_5521 at 278.981 kb on + strand, within Pf1N1B4_5521 at 278.981 kb on + strand, within Pf1N1B4_5521 at 278.982 kb on - strand, within Pf1N1B4_5521 at 278.982 kb on - strand, within Pf1N1B4_5521 at 278.982 kb on - strand, within Pf1N1B4_5521 at 278.982 kb on - strand, within Pf1N1B4_5521 at 278.982 kb on - strand, within Pf1N1B4_5521 at 279.048 kb on + strand, within Pf1N1B4_5521 at 279.049 kb on - strand, within Pf1N1B4_5521 at 279.049 kb on - strand, within Pf1N1B4_5521 at 279.264 kb on + strand, within Pf1N1B4_5521 at 279.282 kb on + strand, within Pf1N1B4_5521 at 279.282 kb on + strand, within Pf1N1B4_5521 at 279.282 kb on + strand, within Pf1N1B4_5521 at 279.282 kb on + strand, within Pf1N1B4_5521 at 279.283 kb on - strand, within Pf1N1B4_5521 at 279.371 kb on + strand, within Pf1N1B4_5521 at 279.371 kb on + strand, within Pf1N1B4_5521 at 279.371 kb on + strand, within Pf1N1B4_5521 at 279.372 kb on - strand at 279.372 kb on - strand, within Pf1N1B4_5521 at 279.423 kb on - strand, within Pf1N1B4_5521 at 279.523 kb on - strand, within Pf1N1B4_5521 at 279.532 kb on + strand, within Pf1N1B4_5521 at 279.532 kb on + strand, within Pf1N1B4_5521 at 279.532 kb on + strand, within Pf1N1B4_5521 at 279.533 kb on - strand, within Pf1N1B4_5521 at 279.533 kb on - strand, within Pf1N1B4_5521 at 279.566 kb on + strand, within Pf1N1B4_5521 at 279.567 kb on - strand, within Pf1N1B4_5521 at 279.570 kb on + strand, within Pf1N1B4_5521 at 279.570 kb on + strand, within Pf1N1B4_5521 at 279.571 kb on - strand, within Pf1N1B4_5521 at 279.571 kb on - strand, within Pf1N1B4_5521 at 279.571 kb on - strand, within Pf1N1B4_5521 at 279.601 kb on + strand at 279.602 kb on - strand at 279.676 kb on - strand at 279.676 kb on - strand at 279.699 kb on + strand at 279.700 kb on - strand at 279.700 kb on - strand at 279.700 kb on - strand at 279.763 kb on + strand at 279.773 kb on + strand at 279.774 kb on - strand at 279.774 kb on - strand at 279.774 kb on - strand at 279.845 kb on + strand at 279.846 kb on - strand at 279.846 kb on - strand at 279.884 kb on - strand, within Pf1N1B4_5522 at 280.065 kb on + strand, within Pf1N1B4_5522 at 280.144 kb on + strand, within Pf1N1B4_5522 at 280.144 kb on + strand, within Pf1N1B4_5522 at 280.144 kb on + strand, within Pf1N1B4_5522 at 280.144 kb on + strand, within Pf1N1B4_5522 at 280.145 kb on - strand, within Pf1N1B4_5522 at 280.145 kb on - strand, within Pf1N1B4_5522 at 280.145 kb on - strand, within Pf1N1B4_5522 at 280.145 kb on - strand, within Pf1N1B4_5522 at 280.145 kb on - strand, within Pf1N1B4_5522 at 280.522 kb on + strand, within Pf1N1B4_5522 at 280.523 kb on - strand, within Pf1N1B4_5522 at 280.523 kb on - strand, within Pf1N1B4_5522 at 280.523 kb on - strand, within Pf1N1B4_5522 at 280.598 kb on + strand, within Pf1N1B4_5522 at 280.599 kb on - strand, within Pf1N1B4_5522 at 280.599 kb on - strand at 280.599 kb on - strand, within Pf1N1B4_5522 at 280.652 kb on + strand at 280.653 kb on - strand at 280.653 kb on - strand at 280.697 kb on - strand at 280.748 kb on - strand at 280.748 kb on - strand at 280.748 kb on - strand at 280.748 kb on - strand at 280.748 kb on - strand at 280.748 kb on - strand at 280.748 kb on - strand at 280.748 kb on - strand at 280.869 kb on + strand at 280.870 kb on - strand at 280.870 kb on - strand at 280.936 kb on + strand at 280.936 kb on + strand at 280.936 kb on + strand at 280.937 kb on - strand at 280.966 kb on + strand at 280.998 kb on + strand, within Pf1N1B4_5523 at 280.998 kb on + strand, within Pf1N1B4_5523 at 281.045 kb on - strand, within Pf1N1B4_5523 at 281.057 kb on + strand, within Pf1N1B4_5523 at 281.058 kb on - strand, within Pf1N1B4_5523 at 281.058 kb on - strand, within Pf1N1B4_5523 at 281.058 kb on - strand, within Pf1N1B4_5523 at 281.122 kb on + strand, within Pf1N1B4_5523 at 281.122 kb on + strand, within Pf1N1B4_5523 at 281.123 kb on - strand, within Pf1N1B4_5523 at 281.123 kb on - strand, within Pf1N1B4_5523 at 281.123 kb on - strand, within Pf1N1B4_5523 at 281.150 kb on + strand, within Pf1N1B4_5523 at 281.151 kb on - strand, within Pf1N1B4_5523 at 281.170 kb on - strand at 281.207 kb on - strand, within Pf1N1B4_5523 at 281.207 kb on - strand, within Pf1N1B4_5523 at 281.350 kb on - strand, within Pf1N1B4_5523 at 281.366 kb on + strand, within Pf1N1B4_5523 at 281.366 kb on + strand, within Pf1N1B4_5523 at 281.366 kb on + strand, within Pf1N1B4_5523 at 281.367 kb on - strand, within Pf1N1B4_5523 at 281.374 kb on + strand, within Pf1N1B4_5523 at 281.386 kb on + strand, within Pf1N1B4_5523 at 281.386 kb on + strand, within Pf1N1B4_5523 at 281.386 kb on + strand, within Pf1N1B4_5523 at 281.387 kb on - strand, within Pf1N1B4_5523 at 281.387 kb on - strand, within Pf1N1B4_5523 at 281.387 kb on - strand, within Pf1N1B4_5523 at 281.394 kb on + strand, within Pf1N1B4_5523 at 281.394 kb on + strand, within Pf1N1B4_5523 at 281.507 kb on - strand, within Pf1N1B4_5523 at 281.528 kb on + strand at 281.547 kb on + strand, within Pf1N1B4_5523 at 281.548 kb on - strand, within Pf1N1B4_5523 at 281.571 kb on + strand, within Pf1N1B4_5523 at 281.571 kb on + strand, within Pf1N1B4_5523 at 281.585 kb on - strand, within Pf1N1B4_5523 at 281.642 kb on - strand, within Pf1N1B4_5523 at 281.642 kb on - strand, within Pf1N1B4_5523 at 281.650 kb on + strand, within Pf1N1B4_5523 at 281.651 kb on - strand, within Pf1N1B4_5523 at 281.651 kb on - strand, within Pf1N1B4_5523 at 281.651 kb on - strand, within Pf1N1B4_5523 at 281.651 kb on - strand, within Pf1N1B4_5523 at 281.651 kb on - strand, within Pf1N1B4_5523 at 281.651 kb on - strand, within Pf1N1B4_5523 at 281.651 kb on - strand, within Pf1N1B4_5523
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t1 remove 278,794 + Pf1N1B4_5521 0.41 -0.0 278,794 + Pf1N1B4_5521 0.41 -0.0 278,849 + Pf1N1B4_5521 0.45 -0.0 278,850 - Pf1N1B4_5521 0.45 -1.6 278,850 - Pf1N1B4_5521 0.45 -0.0 278,862 + Pf1N1B4_5521 0.45 -0.0 278,862 + Pf1N1B4_5521 0.45 -0.0 278,907 - Pf1N1B4_5521 0.48 -1.2 278,917 + Pf1N1B4_5521 0.49 -0.0 278,981 + -0.0 278,981 + Pf1N1B4_5521 0.53 -0.0 278,981 + Pf1N1B4_5521 0.53 -1.8 278,981 + Pf1N1B4_5521 0.53 -0.0 278,981 + Pf1N1B4_5521 0.53 -0.0 278,982 - Pf1N1B4_5521 0.53 -2.3 278,982 - Pf1N1B4_5521 0.53 -0.0 278,982 - Pf1N1B4_5521 0.53 -0.0 278,982 - Pf1N1B4_5521 0.53 -3.1 278,982 - Pf1N1B4_5521 0.53 -2.5 279,048 + Pf1N1B4_5521 0.57 -2.1 279,049 - Pf1N1B4_5521 0.57 -0.0 279,049 - Pf1N1B4_5521 0.57 -0.0 279,264 + Pf1N1B4_5521 0.70 -0.0 279,282 + Pf1N1B4_5521 0.71 -3.9 279,282 + Pf1N1B4_5521 0.71 -0.0 279,282 + Pf1N1B4_5521 0.71 -0.0 279,282 + Pf1N1B4_5521 0.71 -3.6 279,283 - Pf1N1B4_5521 0.71 -0.0 279,371 + Pf1N1B4_5521 0.76 -0.0 279,371 + Pf1N1B4_5521 0.76 -0.0 279,371 + Pf1N1B4_5521 0.76 -0.0 279,372 - -0.0 279,372 - Pf1N1B4_5521 0.76 -0.0 279,423 - Pf1N1B4_5521 0.79 -0.0 279,523 - Pf1N1B4_5521 0.85 -0.0 279,532 + Pf1N1B4_5521 0.86 -0.0 279,532 + Pf1N1B4_5521 0.86 -1.1 279,532 + Pf1N1B4_5521 0.86 -5.4 279,533 - Pf1N1B4_5521 0.86 -1.6 279,533 - Pf1N1B4_5521 0.86 -0.0 279,566 + Pf1N1B4_5521 0.88 -0.0 279,567 - Pf1N1B4_5521 0.88 -0.0 279,570 + Pf1N1B4_5521 0.88 -0.0 279,570 + Pf1N1B4_5521 0.88 -0.0 279,571 - Pf1N1B4_5521 0.88 -0.0 279,571 - Pf1N1B4_5521 0.88 -0.0 279,571 - Pf1N1B4_5521 0.88 -0.0 279,601 + -0.0 279,602 - -1.5 279,676 - -1.9 279,676 - -0.0 279,699 + -0.0 279,700 - -0.0 279,700 - -0.0 279,700 - -2.6 279,763 + -0.0 279,773 + -0.0 279,774 - -0.0 279,774 - -3.0 279,774 - -0.0 279,845 + -3.5 279,846 - -0.0 279,846 - -1.7 279,884 - Pf1N1B4_5522 0.12 -0.0 280,065 + Pf1N1B4_5522 0.31 -3.1 280,144 + Pf1N1B4_5522 0.39 -0.0 280,144 + Pf1N1B4_5522 0.39 -3.2 280,144 + Pf1N1B4_5522 0.39 -3.0 280,144 + Pf1N1B4_5522 0.39 -0.0 280,145 - Pf1N1B4_5522 0.40 -1.5 280,145 - Pf1N1B4_5522 0.40 -0.0 280,145 - Pf1N1B4_5522 0.40 -0.0 280,145 - Pf1N1B4_5522 0.40 -0.0 280,145 - Pf1N1B4_5522 0.40 -0.0 280,522 + Pf1N1B4_5522 0.80 -1.6 280,523 - Pf1N1B4_5522 0.80 -2.4 280,523 - Pf1N1B4_5522 0.80 -0.0 280,523 - Pf1N1B4_5522 0.80 -0.0 280,598 + Pf1N1B4_5522 0.88 -3.5 280,599 - Pf1N1B4_5522 0.88 -0.0 280,599 - -1.2 280,599 - Pf1N1B4_5522 0.88 -2.4 280,652 + -5.0 280,653 - -0.0 280,653 - -0.0 280,697 - -0.0 280,748 - -0.0 280,748 - -0.0 280,748 - -0.0 280,748 - -2.3 280,748 - -0.0 280,748 - -0.0 280,748 - -0.0 280,748 - -0.8 280,869 + -0.0 280,870 - -0.0 280,870 - -0.0 280,936 + -0.0 280,936 + -1.6 280,936 + -2.7 280,937 - -2.2 280,966 + -0.0 280,998 + Pf1N1B4_5523 0.12 -0.0 280,998 + Pf1N1B4_5523 0.12 -2.0 281,045 - Pf1N1B4_5523 0.15 -0.0 281,057 + Pf1N1B4_5523 0.16 -2.8 281,058 - Pf1N1B4_5523 0.16 -3.3 281,058 - Pf1N1B4_5523 0.16 -0.0 281,058 - Pf1N1B4_5523 0.16 -3.0 281,122 + Pf1N1B4_5523 0.20 -0.0 281,122 + Pf1N1B4_5523 0.20 -0.0 281,123 - Pf1N1B4_5523 0.20 -2.1 281,123 - Pf1N1B4_5523 0.20 -0.0 281,123 - Pf1N1B4_5523 0.20 -0.0 281,150 + Pf1N1B4_5523 0.22 -0.0 281,151 - Pf1N1B4_5523 0.22 -3.4 281,170 - -0.0 281,207 - Pf1N1B4_5523 0.25 -3.4 281,207 - Pf1N1B4_5523 0.25 -0.0 281,350 - Pf1N1B4_5523 0.34 -2.2 281,366 + Pf1N1B4_5523 0.35 -0.0 281,366 + Pf1N1B4_5523 0.35 -0.0 281,366 + Pf1N1B4_5523 0.35 -0.0 281,367 - Pf1N1B4_5523 0.35 -0.0 281,374 + Pf1N1B4_5523 0.36 -3.6 281,386 + Pf1N1B4_5523 0.37 -3.2 281,386 + Pf1N1B4_5523 0.37 -0.0 281,386 + Pf1N1B4_5523 0.37 -0.0 281,387 - Pf1N1B4_5523 0.37 -0.0 281,387 - Pf1N1B4_5523 0.37 -0.0 281,387 - Pf1N1B4_5523 0.37 -1.4 281,394 + Pf1N1B4_5523 0.37 -0.0 281,394 + Pf1N1B4_5523 0.37 -0.0 281,507 - Pf1N1B4_5523 0.44 -0.0 281,528 + -0.0 281,547 + Pf1N1B4_5523 0.47 -0.0 281,548 - Pf1N1B4_5523 0.47 -0.0 281,571 + Pf1N1B4_5523 0.48 -2.7 281,571 + Pf1N1B4_5523 0.48 -0.0 281,585 - Pf1N1B4_5523 0.49 -0.0 281,642 - Pf1N1B4_5523 0.53 -1.3 281,642 - Pf1N1B4_5523 0.53 -0.0 281,650 + Pf1N1B4_5523 0.53 -0.0 281,651 - Pf1N1B4_5523 0.53 -1.4 281,651 - Pf1N1B4_5523 0.53 -0.0 281,651 - Pf1N1B4_5523 0.53 -0.0 281,651 - Pf1N1B4_5523 0.53 -2.7 281,651 - Pf1N1B4_5523 0.53 -0.0 281,651 - Pf1N1B4_5523 0.53 -1.8 281,651 - Pf1N1B4_5523 0.53 -0.0
Or see this region's nucleotide sequence