Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5299

Experiment: Nitrate reduction, replicate C t1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5296 and Pf1N1B4_5297 are separated by 152 nucleotidesPf1N1B4_5297 and Pf1N1B4_5298 are separated by 20 nucleotidesPf1N1B4_5298 and Pf1N1B4_5299 are separated by 38 nucleotidesPf1N1B4_5299 and Pf1N1B4_5300 are separated by 78 nucleotides Pf1N1B4_5296 - hypothetical protein, at 50,875 to 51,123 _5296 Pf1N1B4_5297 - hypothetical protein, at 51,276 to 51,413 _5297 Pf1N1B4_5298 - hypothetical protein, at 51,434 to 51,667 _5298 Pf1N1B4_5299 - hypothetical protein, at 51,706 to 51,963 _5299 Pf1N1B4_5300 - hypothetical protein, at 52,042 to 54,108 _5300 Position (kb) 51 52Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 50.791 kb on + strandat 50.974 kb on + strand, within Pf1N1B4_5296at 50.974 kb on + strand, within Pf1N1B4_5296at 50.974 kb on + strand, within Pf1N1B4_5296at 50.974 kb on + strandat 50.974 kb on + strand, within Pf1N1B4_5296at 50.974 kb on + strandat 50.974 kb on + strand, within Pf1N1B4_5296at 50.974 kb on + strand, within Pf1N1B4_5296at 50.974 kb on + strand, within Pf1N1B4_5296at 50.975 kb on - strand, within Pf1N1B4_5296at 50.975 kb on - strand, within Pf1N1B4_5296at 50.975 kb on - strand, within Pf1N1B4_5296at 51.001 kb on + strand, within Pf1N1B4_5296at 51.001 kb on + strand, within Pf1N1B4_5296at 51.002 kb on - strand, within Pf1N1B4_5296at 51.034 kb on - strand, within Pf1N1B4_5296at 51.166 kb on - strandat 51.166 kb on - strandat 51.373 kb on + strand, within Pf1N1B4_5297at 51.377 kb on + strand, within Pf1N1B4_5297at 51.377 kb on + strand, within Pf1N1B4_5297at 51.452 kb on + strandat 51.452 kb on + strandat 51.452 kb on + strandat 51.530 kb on - strandat 51.537 kb on + strand, within Pf1N1B4_5298at 51.538 kb on - strand, within Pf1N1B4_5298at 51.538 kb on - strand, within Pf1N1B4_5298at 51.539 kb on + strand, within Pf1N1B4_5298at 51.539 kb on + strand, within Pf1N1B4_5298at 51.539 kb on + strand, within Pf1N1B4_5298at 51.539 kb on + strandat 51.539 kb on + strand, within Pf1N1B4_5298at 51.539 kb on + strand, within Pf1N1B4_5298at 51.539 kb on + strand, within Pf1N1B4_5298at 51.539 kb on + strand, within Pf1N1B4_5298at 51.539 kb on + strand, within Pf1N1B4_5298at 51.539 kb on + strand, within Pf1N1B4_5298at 51.540 kb on - strand, within Pf1N1B4_5298at 51.540 kb on - strand, within Pf1N1B4_5298at 51.540 kb on - strand, within Pf1N1B4_5298at 51.540 kb on - strand, within Pf1N1B4_5298at 51.540 kb on - strand, within Pf1N1B4_5298at 51.540 kb on - strand, within Pf1N1B4_5298at 51.540 kb on - strand, within Pf1N1B4_5298at 51.540 kb on - strand, within Pf1N1B4_5298at 51.540 kb on - strand, within Pf1N1B4_5298at 51.540 kb on - strand, within Pf1N1B4_5298at 51.540 kb on - strand, within Pf1N1B4_5298at 51.540 kb on - strand, within Pf1N1B4_5298at 51.613 kb on - strand, within Pf1N1B4_5298at 51.685 kb on + strandat 51.685 kb on + strandat 51.686 kb on - strandat 51.695 kb on + strandat 51.695 kb on + strandat 51.695 kb on + strandat 51.695 kb on + strandat 51.695 kb on + strandat 51.696 kb on - strandat 51.696 kb on - strandat 51.699 kb on + strandat 51.699 kb on + strandat 51.699 kb on + strandat 51.699 kb on + strandat 51.700 kb on - strandat 51.700 kb on - strandat 51.821 kb on + strand, within Pf1N1B4_5299at 51.831 kb on + strand, within Pf1N1B4_5299at 51.832 kb on + strandat 51.832 kb on + strand, within Pf1N1B4_5299at 51.832 kb on + strand, within Pf1N1B4_5299at 51.832 kb on + strand, within Pf1N1B4_5299at 51.885 kb on - strandat 51.885 kb on - strand, within Pf1N1B4_5299at 51.931 kb on + strand, within Pf1N1B4_5299at 51.932 kb on - strand, within Pf1N1B4_5299at 52.040 kb on - strandat 52.075 kb on + strandat 52.253 kb on - strand, within Pf1N1B4_5300at 52.253 kb on - strand, within Pf1N1B4_5300at 52.312 kb on - strand, within Pf1N1B4_5300at 52.520 kb on + strand, within Pf1N1B4_5300at 52.520 kb on + strand, within Pf1N1B4_5300at 52.520 kb on + strand, within Pf1N1B4_5300at 52.520 kb on + strand, within Pf1N1B4_5300at 52.521 kb on - strand, within Pf1N1B4_5300at 52.521 kb on - strand, within Pf1N1B4_5300at 52.521 kb on - strand, within Pf1N1B4_5300at 52.521 kb on - strand, within Pf1N1B4_5300at 52.777 kb on + strand, within Pf1N1B4_5300at 52.778 kb on - strand, within Pf1N1B4_5300at 52.778 kb on - strand, within Pf1N1B4_5300at 52.778 kb on - strand, within Pf1N1B4_5300at 52.867 kb on + strand, within Pf1N1B4_5300at 52.867 kb on + strand, within Pf1N1B4_5300at 52.867 kb on + strand, within Pf1N1B4_5300at 52.868 kb on - strand, within Pf1N1B4_5300at 52.868 kb on - strand, within Pf1N1B4_5300

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t1
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50,791 + -3.7
50,974 + Pf1N1B4_5296 0.40 -2.7
50,974 + Pf1N1B4_5296 0.40 -0.0
50,974 + Pf1N1B4_5296 0.40 -3.2
50,974 + -4.0
50,974 + Pf1N1B4_5296 0.40 -0.0
50,974 + -2.1
50,974 + Pf1N1B4_5296 0.40 -2.1
50,974 + Pf1N1B4_5296 0.40 -0.0
50,974 + Pf1N1B4_5296 0.40 -0.0
50,975 - Pf1N1B4_5296 0.40 -3.1
50,975 - Pf1N1B4_5296 0.40 -2.3
50,975 - Pf1N1B4_5296 0.40 -0.0
51,001 + Pf1N1B4_5296 0.51 -0.0
51,001 + Pf1N1B4_5296 0.51 -1.6
51,002 - Pf1N1B4_5296 0.51 -2.3
51,034 - Pf1N1B4_5296 0.64 -3.2
51,166 - -0.0
51,166 - -0.0
51,373 + Pf1N1B4_5297 0.70 -0.0
51,377 + Pf1N1B4_5297 0.73 -0.0
51,377 + Pf1N1B4_5297 0.73 -0.0
51,452 + -0.0
51,452 + -2.9
51,452 + -2.6
51,530 - -0.0
51,537 + Pf1N1B4_5298 0.44 -0.0
51,538 - Pf1N1B4_5298 0.44 -0.0
51,538 - Pf1N1B4_5298 0.44 -2.9
51,539 + Pf1N1B4_5298 0.45 -0.0
51,539 + Pf1N1B4_5298 0.45 -2.2
51,539 + Pf1N1B4_5298 0.45 -0.0
51,539 + -4.4
51,539 + Pf1N1B4_5298 0.45 -1.5
51,539 + Pf1N1B4_5298 0.45 -3.0
51,539 + Pf1N1B4_5298 0.45 -0.0
51,539 + Pf1N1B4_5298 0.45 -0.0
51,539 + Pf1N1B4_5298 0.45 -3.0
51,539 + Pf1N1B4_5298 0.45 -0.0
51,540 - Pf1N1B4_5298 0.45 -0.0
51,540 - Pf1N1B4_5298 0.45 -0.0
51,540 - Pf1N1B4_5298 0.45 -0.0
51,540 - Pf1N1B4_5298 0.45 -0.0
51,540 - Pf1N1B4_5298 0.45 -0.0
51,540 - Pf1N1B4_5298 0.45 -1.5
51,540 - Pf1N1B4_5298 0.45 -3.1
51,540 - Pf1N1B4_5298 0.45 -0.0
51,540 - Pf1N1B4_5298 0.45 -2.3
51,540 - Pf1N1B4_5298 0.45 -0.0
51,540 - Pf1N1B4_5298 0.45 -0.0
51,540 - Pf1N1B4_5298 0.45 -2.1
51,613 - Pf1N1B4_5298 0.76 +0.2
51,685 + -0.0
51,685 + -0.0
51,686 - -1.2
51,695 + -3.0
51,695 + -0.0
51,695 + -1.9
51,695 + -0.6
51,695 + -0.0
51,696 - -0.0
51,696 - -0.0
51,699 + -2.6
51,699 + -0.0
51,699 + -2.0
51,699 + -0.0
51,700 - -0.0
51,700 - -0.0
51,821 + Pf1N1B4_5299 0.45 -3.4
51,831 + Pf1N1B4_5299 0.48 -0.0
51,832 + -3.8
51,832 + Pf1N1B4_5299 0.49 -0.0
51,832 + Pf1N1B4_5299 0.49 -0.0
51,832 + Pf1N1B4_5299 0.49 -2.4
51,885 - -1.2
51,885 - Pf1N1B4_5299 0.69 -2.6
51,931 + Pf1N1B4_5299 0.87 -3.5
51,932 - Pf1N1B4_5299 0.88 -0.0
52,040 - -0.0
52,075 + -0.0
52,253 - Pf1N1B4_5300 0.10 -0.0
52,253 - Pf1N1B4_5300 0.10 -2.0
52,312 - Pf1N1B4_5300 0.13 -1.3
52,520 + Pf1N1B4_5300 0.23 -2.7
52,520 + Pf1N1B4_5300 0.23 -0.0
52,520 + Pf1N1B4_5300 0.23 -0.0
52,520 + Pf1N1B4_5300 0.23 -0.7
52,521 - Pf1N1B4_5300 0.23 -2.1
52,521 - Pf1N1B4_5300 0.23 -0.0
52,521 - Pf1N1B4_5300 0.23 -2.7
52,521 - Pf1N1B4_5300 0.23 -0.0
52,777 + Pf1N1B4_5300 0.36 -1.4
52,778 - Pf1N1B4_5300 0.36 -0.0
52,778 - Pf1N1B4_5300 0.36 -3.6
52,778 - Pf1N1B4_5300 0.36 -2.0
52,867 + Pf1N1B4_5300 0.40 -1.8
52,867 + Pf1N1B4_5300 0.40 -2.3
52,867 + Pf1N1B4_5300 0.40 -0.0
52,868 - Pf1N1B4_5300 0.40 -2.0
52,868 - Pf1N1B4_5300 0.40 -0.0

Or see this region's nucleotide sequence