Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5036

Experiment: Nitrate reduction, replicate C t1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5034 and Pf1N1B4_5035 are separated by 306 nucleotidesPf1N1B4_5035 and Pf1N1B4_5036 are separated by 233 nucleotidesPf1N1B4_5036 and Pf1N1B4_5236 are separated by 165 nucleotidesPf1N1B4_5236 and Pf1N1B4_5237 are separated by 198 nucleotidesPf1N1B4_5237 and Pf1N1B4_5037 are separated by 282 nucleotides Pf1N1B4_5034 - hypothetical protein, at 5,579,569 to 5,579,799 _5034 Pf1N1B4_5035 - hypothetical protein, at 5,580,106 to 5,580,390 _5035 Pf1N1B4_5036 - hypothetical protein, at 5,580,624 to 5,580,863 _5036 Pf1N1B4_5236 - tRNA-Leu-TAA, at 5,581,029 to 5,581,112 _5236 Pf1N1B4_5237 - tRNA-Cys-GCA, at 5,581,311 to 5,581,381 _5237 Pf1N1B4_5037 - Transcriptional regulator of various polyols utilization, AraC family, at 5,581,664 to 5,582,572 _5037 Position (kb) 5580 5581Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 5579.772 kb on - strand, within Pf1N1B4_5034at 5579.875 kb on + strandat 5579.876 kb on - strandat 5579.876 kb on - strandat 5580.044 kb on + strandat 5580.044 kb on + strandat 5580.044 kb on + strandat 5580.045 kb on - strandat 5580.045 kb on - strandat 5580.179 kb on + strand, within Pf1N1B4_5035at 5580.180 kb on - strand, within Pf1N1B4_5035at 5580.180 kb on - strandat 5580.388 kb on + strandat 5580.434 kb on - strandat 5580.434 kb on - strandat 5580.562 kb on - strandat 5580.590 kb on + strandat 5580.590 kb on + strandat 5580.590 kb on + strandat 5580.786 kb on + strandat 5580.786 kb on + strand, within Pf1N1B4_5036at 5580.787 kb on - strand, within Pf1N1B4_5036at 5580.787 kb on - strand, within Pf1N1B4_5036at 5580.787 kb on - strandat 5580.787 kb on - strand, within Pf1N1B4_5036at 5580.787 kb on - strand, within Pf1N1B4_5036at 5580.789 kb on + strand, within Pf1N1B4_5036at 5580.789 kb on + strand, within Pf1N1B4_5036at 5580.789 kb on + strand, within Pf1N1B4_5036at 5580.789 kb on + strand, within Pf1N1B4_5036at 5580.790 kb on - strand, within Pf1N1B4_5036at 5580.790 kb on - strand, within Pf1N1B4_5036at 5580.793 kb on - strand, within Pf1N1B4_5036at 5580.861 kb on + strandat 5580.861 kb on + strandat 5580.861 kb on + strandat 5580.897 kb on - strandat 5581.191 kb on - strandat 5581.191 kb on - strandat 5581.191 kb on - strandat 5581.195 kb on - strandat 5581.278 kb on - strandat 5581.786 kb on + strand, within Pf1N1B4_5037

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t1
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5,579,772 - Pf1N1B4_5034 0.88 -2.3
5,579,875 + -2.2
5,579,876 - -2.1
5,579,876 - +0.1
5,580,044 + +0.1
5,580,044 + +0.1
5,580,044 + +0.1
5,580,045 - -3.1
5,580,045 - -3.8
5,580,179 + Pf1N1B4_5035 0.26 +0.1
5,580,180 - Pf1N1B4_5035 0.26 +0.1
5,580,180 - -1.9
5,580,388 + -2.6
5,580,434 - -3.8
5,580,434 - +0.1
5,580,562 - +0.1
5,580,590 + -2.1
5,580,590 + -0.2
5,580,590 + +0.1
5,580,786 + +0.1
5,580,786 + Pf1N1B4_5036 0.68 -4.3
5,580,787 - Pf1N1B4_5036 0.68 +0.1
5,580,787 - Pf1N1B4_5036 0.68 -3.3
5,580,787 - -1.9
5,580,787 - Pf1N1B4_5036 0.68 +0.1
5,580,787 - Pf1N1B4_5036 0.68 +0.1
5,580,789 + Pf1N1B4_5036 0.69 -3.7
5,580,789 + Pf1N1B4_5036 0.69 -2.3
5,580,789 + Pf1N1B4_5036 0.69 -2.7
5,580,789 + Pf1N1B4_5036 0.69 +0.1
5,580,790 - Pf1N1B4_5036 0.69 +0.1
5,580,790 - Pf1N1B4_5036 0.69 -2.1
5,580,793 - Pf1N1B4_5036 0.70 +0.1
5,580,861 + +0.1
5,580,861 + -2.0
5,580,861 + -2.3
5,580,897 - -0.9
5,581,191 - -2.7
5,581,191 - +0.1
5,581,191 - +0.1
5,581,195 - -2.3
5,581,278 - +0.1
5,581,786 + Pf1N1B4_5037 0.13 +0.1

Or see this region's nucleotide sequence