Experiment: Nitrate reduction, replicate C t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5034 and Pf1N1B4_5035 are separated by 306 nucleotides Pf1N1B4_5035 and Pf1N1B4_5036 are separated by 233 nucleotides Pf1N1B4_5036 and Pf1N1B4_5236 are separated by 165 nucleotides Pf1N1B4_5236 and Pf1N1B4_5237 are separated by 198 nucleotides Pf1N1B4_5237 and Pf1N1B4_5037 are separated by 282 nucleotides
Pf1N1B4_5034 - hypothetical protein, at 5,579,569 to 5,579,799
_5034
Pf1N1B4_5035 - hypothetical protein, at 5,580,106 to 5,580,390
_5035
Pf1N1B4_5036 - hypothetical protein, at 5,580,624 to 5,580,863
_5036
Pf1N1B4_5236 - tRNA-Leu-TAA, at 5,581,029 to 5,581,112
_5236
Pf1N1B4_5237 - tRNA-Cys-GCA, at 5,581,311 to 5,581,381
_5237
Pf1N1B4_5037 - Transcriptional regulator of various polyols utilization, AraC family, at 5,581,664 to 5,582,572
_5037
Position (kb)
5580
5581 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 5579.772 kb on - strand, within Pf1N1B4_5034 at 5579.875 kb on + strand at 5579.876 kb on - strand at 5579.876 kb on - strand at 5580.044 kb on + strand at 5580.044 kb on + strand at 5580.044 kb on + strand at 5580.045 kb on - strand at 5580.045 kb on - strand at 5580.179 kb on + strand, within Pf1N1B4_5035 at 5580.180 kb on - strand, within Pf1N1B4_5035 at 5580.180 kb on - strand at 5580.388 kb on + strand at 5580.434 kb on - strand at 5580.434 kb on - strand at 5580.562 kb on - strand at 5580.590 kb on + strand at 5580.590 kb on + strand at 5580.590 kb on + strand at 5580.786 kb on + strand at 5580.786 kb on + strand, within Pf1N1B4_5036 at 5580.787 kb on - strand, within Pf1N1B4_5036 at 5580.787 kb on - strand, within Pf1N1B4_5036 at 5580.787 kb on - strand at 5580.787 kb on - strand, within Pf1N1B4_5036 at 5580.787 kb on - strand, within Pf1N1B4_5036 at 5580.789 kb on + strand, within Pf1N1B4_5036 at 5580.789 kb on + strand, within Pf1N1B4_5036 at 5580.789 kb on + strand, within Pf1N1B4_5036 at 5580.789 kb on + strand, within Pf1N1B4_5036 at 5580.790 kb on - strand, within Pf1N1B4_5036 at 5580.790 kb on - strand, within Pf1N1B4_5036 at 5580.793 kb on - strand, within Pf1N1B4_5036 at 5580.861 kb on + strand at 5580.861 kb on + strand at 5580.861 kb on + strand at 5580.897 kb on - strand at 5581.191 kb on - strand at 5581.191 kb on - strand at 5581.191 kb on - strand at 5581.195 kb on - strand at 5581.278 kb on - strand at 5581.786 kb on + strand, within Pf1N1B4_5037
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t1 remove 5,579,772 - Pf1N1B4_5034 0.88 -2.3 5,579,875 + -2.2 5,579,876 - -2.1 5,579,876 - +0.1 5,580,044 + +0.1 5,580,044 + +0.1 5,580,044 + +0.1 5,580,045 - -3.1 5,580,045 - -3.8 5,580,179 + Pf1N1B4_5035 0.26 +0.1 5,580,180 - Pf1N1B4_5035 0.26 +0.1 5,580,180 - -1.9 5,580,388 + -2.6 5,580,434 - -3.8 5,580,434 - +0.1 5,580,562 - +0.1 5,580,590 + -2.1 5,580,590 + -0.2 5,580,590 + +0.1 5,580,786 + +0.1 5,580,786 + Pf1N1B4_5036 0.68 -4.3 5,580,787 - Pf1N1B4_5036 0.68 +0.1 5,580,787 - Pf1N1B4_5036 0.68 -3.3 5,580,787 - -1.9 5,580,787 - Pf1N1B4_5036 0.68 +0.1 5,580,787 - Pf1N1B4_5036 0.68 +0.1 5,580,789 + Pf1N1B4_5036 0.69 -3.7 5,580,789 + Pf1N1B4_5036 0.69 -2.3 5,580,789 + Pf1N1B4_5036 0.69 -2.7 5,580,789 + Pf1N1B4_5036 0.69 +0.1 5,580,790 - Pf1N1B4_5036 0.69 +0.1 5,580,790 - Pf1N1B4_5036 0.69 -2.1 5,580,793 - Pf1N1B4_5036 0.70 +0.1 5,580,861 + +0.1 5,580,861 + -2.0 5,580,861 + -2.3 5,580,897 - -0.9 5,581,191 - -2.7 5,581,191 - +0.1 5,581,191 - +0.1 5,581,195 - -2.3 5,581,278 - +0.1 5,581,786 + Pf1N1B4_5037 0.13 +0.1
Or see this region's nucleotide sequence