Experiment: Nitrate reduction, replicate C t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4952 and Pf1N1B4_4953 overlap by 4 nucleotides Pf1N1B4_4953 and Pf1N1B4_4954 are separated by 891 nucleotides
Pf1N1B4_4952 - L-seryl-tRNA(Sec) selenium transferase (EC 2.9.1.1), at 5,488,640 to 5,490,070
_4952
Pf1N1B4_4953 - Selenocysteine-specific translation elongation factor, at 5,490,067 to 5,491,986
_4953
Pf1N1B4_4954 - Enoyl-[acyl-carrier-protein] reductase [NADPH] (EC 1.3.1.10), at 5,492,878 to 5,493,624
_4954
Position (kb)
5490
5491
5492 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5489.560 kb on - strand, within Pf1N1B4_4952 at 5489.779 kb on - strand, within Pf1N1B4_4952 at 5489.779 kb on - strand, within Pf1N1B4_4952 at 5490.191 kb on - strand at 5490.192 kb on + strand at 5490.192 kb on + strand at 5490.193 kb on - strand at 5490.196 kb on + strand at 5490.393 kb on + strand, within Pf1N1B4_4953 at 5491.182 kb on - strand, within Pf1N1B4_4953 at 5491.517 kb on + strand, within Pf1N1B4_4953 at 5491.518 kb on - strand, within Pf1N1B4_4953 at 5491.617 kb on - strand, within Pf1N1B4_4953 at 5491.898 kb on - strand at 5492.113 kb on - strand at 5492.116 kb on + strand at 5492.131 kb on + strand at 5492.354 kb on + strand at 5492.355 kb on - strand at 5492.416 kb on - strand at 5492.417 kb on - strand at 5492.451 kb on - strand at 5492.472 kb on + strand at 5492.473 kb on - strand at 5492.565 kb on - strand at 5492.593 kb on + strand at 5492.594 kb on - strand at 5492.627 kb on - strand at 5492.729 kb on - strand at 5492.729 kb on - strand at 5492.813 kb on - strand at 5492.853 kb on + strand at 5492.854 kb on - strand at 5492.854 kb on - strand at 5492.854 kb on - strand at 5492.854 kb on - strand at 5492.854 kb on - strand at 5492.880 kb on - strand at 5492.904 kb on + strand at 5492.905 kb on - strand at 5492.905 kb on - strand at 5492.961 kb on + strand, within Pf1N1B4_4954 at 5492.965 kb on + strand, within Pf1N1B4_4954
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t1 remove 5,489,560 - Pf1N1B4_4952 0.64 -3.7 5,489,779 - Pf1N1B4_4952 0.80 +0.1 5,489,779 - Pf1N1B4_4952 0.80 +0.1 5,490,191 - +0.1 5,490,192 + +0.1 5,490,192 + -1.8 5,490,193 - -2.4 5,490,196 + +0.1 5,490,393 + Pf1N1B4_4953 0.17 +0.1 5,491,182 - Pf1N1B4_4953 0.58 +0.1 5,491,517 + Pf1N1B4_4953 0.76 -3.3 5,491,518 - Pf1N1B4_4953 0.76 -2.8 5,491,617 - Pf1N1B4_4953 0.81 +0.1 5,491,898 - -2.5 5,492,113 - +0.1 5,492,116 + +0.1 5,492,131 + +0.1 5,492,354 + +0.1 5,492,355 - -2.0 5,492,416 - +0.1 5,492,417 - -2.9 5,492,451 - -2.9 5,492,472 + -2.0 5,492,473 - -2.7 5,492,565 - -2.4 5,492,593 + +0.1 5,492,594 - -1.4 5,492,627 - -2.3 5,492,729 - +0.1 5,492,729 - -3.2 5,492,813 - -2.0 5,492,853 + +0.1 5,492,854 - +0.1 5,492,854 - +0.1 5,492,854 - -1.8 5,492,854 - -2.4 5,492,854 - +0.1 5,492,880 - -2.1 5,492,904 + +0.1 5,492,905 - -2.0 5,492,905 - -1.4 5,492,961 + Pf1N1B4_4954 0.11 +0.1 5,492,965 + Pf1N1B4_4954 0.12 +0.1
Or see this region's nucleotide sequence