Experiment: Nitrate reduction, replicate C t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4566 and Pf1N1B4_4567 are separated by 161 nucleotides Pf1N1B4_4567 and Pf1N1B4_4568 are separated by 107 nucleotides Pf1N1B4_4568 and Pf1N1B4_4569 are separated by 195 nucleotides
Pf1N1B4_4566 - Universal stress protein family 7, at 5,039,576 to 5,040,439
_4566
Pf1N1B4_4567 - tRNA-(ms[2]io[6]A)-hydroxylase (EC 1.-.-.-), at 5,040,601 to 5,041,209
_4567
Pf1N1B4_4568 - FIG00793915: hypothetical protein, at 5,041,317 to 5,041,805
_4568
Pf1N1B4_4569 - DNA-3-methyladenine glycosylase II (EC 3.2.2.21), at 5,042,001 to 5,042,618
_4569
Position (kb) 5040
5041
5042 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 5039.674 kb on + strand, within Pf1N1B4_4566 at 5039.677 kb on + strand, within Pf1N1B4_4566 at 5039.677 kb on + strand, within Pf1N1B4_4566 at 5039.678 kb on - strand, within Pf1N1B4_4566 at 5039.678 kb on - strand at 5039.678 kb on - strand at 5039.746 kb on + strand, within Pf1N1B4_4566 at 5039.933 kb on + strand, within Pf1N1B4_4566 at 5039.996 kb on - strand, within Pf1N1B4_4566 at 5039.996 kb on - strand, within Pf1N1B4_4566 at 5040.483 kb on + strand at 5040.483 kb on + strand at 5040.483 kb on + strand at 5040.483 kb on + strand at 5040.483 kb on + strand at 5040.483 kb on + strand at 5040.483 kb on + strand at 5040.484 kb on - strand at 5040.484 kb on - strand at 5040.484 kb on - strand at 5040.485 kb on + strand at 5040.485 kb on + strand at 5040.485 kb on + strand at 5040.485 kb on + strand at 5040.486 kb on - strand at 5040.486 kb on - strand at 5040.486 kb on - strand at 5040.486 kb on - strand at 5040.580 kb on + strand at 5040.751 kb on + strand, within Pf1N1B4_4567 at 5040.752 kb on - strand, within Pf1N1B4_4567 at 5041.027 kb on + strand, within Pf1N1B4_4567 at 5041.028 kb on - strand, within Pf1N1B4_4567 at 5041.090 kb on + strand, within Pf1N1B4_4567 at 5041.091 kb on - strand, within Pf1N1B4_4567 at 5041.215 kb on + strand at 5041.216 kb on - strand at 5041.319 kb on - strand at 5041.530 kb on + strand at 5041.618 kb on + strand at 5041.618 kb on + strand at 5041.619 kb on - strand, within Pf1N1B4_4568 at 5041.619 kb on - strand, within Pf1N1B4_4568 at 5041.741 kb on + strand, within Pf1N1B4_4568 at 5041.960 kb on - strand at 5042.026 kb on + strand at 5042.027 kb on - strand at 5042.084 kb on + strand, within Pf1N1B4_4569 at 5042.084 kb on + strand, within Pf1N1B4_4569 at 5042.179 kb on + strand, within Pf1N1B4_4569 at 5042.180 kb on - strand, within Pf1N1B4_4569
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t1 remove 5,039,674 + Pf1N1B4_4566 0.11 +0.0 5,039,677 + Pf1N1B4_4566 0.12 -0.2 5,039,677 + Pf1N1B4_4566 0.12 +0.0 5,039,678 - Pf1N1B4_4566 0.12 -0.8 5,039,678 - +0.0 5,039,678 - -3.1 5,039,746 + Pf1N1B4_4566 0.20 +0.0 5,039,933 + Pf1N1B4_4566 0.41 +0.0 5,039,996 - Pf1N1B4_4566 0.49 -1.9 5,039,996 - Pf1N1B4_4566 0.49 +1.0 5,040,483 + +0.0 5,040,483 + +0.0 5,040,483 + +0.0 5,040,483 + +0.0 5,040,483 + -1.3 5,040,483 + +0.0 5,040,483 + +0.0 5,040,484 - -1.9 5,040,484 - -1.9 5,040,484 - +0.0 5,040,485 + -1.4 5,040,485 + +0.0 5,040,485 + -1.5 5,040,485 + +0.0 5,040,486 - +0.0 5,040,486 - +0.0 5,040,486 - +0.0 5,040,486 - +0.0 5,040,580 + -1.8 5,040,751 + Pf1N1B4_4567 0.25 -3.0 5,040,752 - Pf1N1B4_4567 0.25 +0.0 5,041,027 + Pf1N1B4_4567 0.70 -2.9 5,041,028 - Pf1N1B4_4567 0.70 +0.0 5,041,090 + Pf1N1B4_4567 0.80 -3.0 5,041,091 - Pf1N1B4_4567 0.80 -3.8 5,041,215 + +0.0 5,041,216 - -1.6 5,041,319 - +0.0 5,041,530 + +0.0 5,041,618 + +0.0 5,041,618 + -4.1 5,041,619 - Pf1N1B4_4568 0.62 -1.3 5,041,619 - Pf1N1B4_4568 0.62 +0.0 5,041,741 + Pf1N1B4_4568 0.87 -1.0 5,041,960 - -0.9 5,042,026 + +0.0 5,042,027 - -1.4 5,042,084 + Pf1N1B4_4569 0.13 -2.2 5,042,084 + Pf1N1B4_4569 0.13 -2.1 5,042,179 + Pf1N1B4_4569 0.29 +0.0 5,042,180 - Pf1N1B4_4569 0.29 -3.8
Or see this region's nucleotide sequence