Experiment: Nitrate reduction, replicate C t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4492 and Pf1N1B4_4493 overlap by 4 nucleotides Pf1N1B4_4493 and Pf1N1B4_4494 are separated by 36 nucleotides Pf1N1B4_4494 and Pf1N1B4_4495 are separated by 159 nucleotides Pf1N1B4_4495 and Pf1N1B4_4496 are separated by 103 nucleotides Pf1N1B4_4496 and Pf1N1B4_4497 are separated by 3 nucleotides
Pf1N1B4_4492 - ABC transporter, ATP-binding protein, at 4,955,010 to 4,955,741
_4492
Pf1N1B4_4493 - FIG00443700: hypothetical protein, at 4,955,738 to 4,956,697
_4493
Pf1N1B4_4494 - hypothetical protein, at 4,956,734 to 4,957,918
_4494
Pf1N1B4_4495 - hypothetical protein, at 4,958,078 to 4,958,206
_4495
Pf1N1B4_4496 - Cytochrome C550 (Soluble cytochrome C), at 4,958,310 to 4,958,771
_4496
Pf1N1B4_4497 - amino acid ABC transporter, periplasmic substrate-binding protein, at 4,958,775 to 4,959,650
_4497
Position (kb)
4956
4957
4958 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4955.873 kb on - strand, within Pf1N1B4_4493 at 4955.982 kb on + strand, within Pf1N1B4_4493 at 4956.128 kb on + strand, within Pf1N1B4_4493 at 4956.321 kb on - strand, within Pf1N1B4_4493 at 4956.378 kb on + strand, within Pf1N1B4_4493 at 4956.378 kb on + strand at 4956.378 kb on + strand, within Pf1N1B4_4493 at 4956.379 kb on - strand at 4956.379 kb on - strand, within Pf1N1B4_4493 at 4956.379 kb on - strand, within Pf1N1B4_4493 at 4956.379 kb on - strand, within Pf1N1B4_4493 at 4956.379 kb on - strand at 4956.504 kb on + strand, within Pf1N1B4_4493 at 4956.505 kb on - strand, within Pf1N1B4_4493 at 4956.505 kb on - strand, within Pf1N1B4_4493 at 4956.947 kb on - strand, within Pf1N1B4_4494 at 4957.176 kb on + strand, within Pf1N1B4_4494 at 4957.674 kb on + strand at 4957.887 kb on + strand at 4957.900 kb on - strand at 4957.949 kb on + strand at 4957.974 kb on + strand at 4957.986 kb on - strand at 4958.007 kb on + strand at 4958.008 kb on - strand at 4958.010 kb on + strand at 4958.045 kb on + strand at 4958.045 kb on + strand at 4958.045 kb on + strand at 4958.190 kb on + strand, within Pf1N1B4_4495 at 4958.193 kb on + strand, within Pf1N1B4_4495 at 4958.194 kb on - strand at 4958.194 kb on - strand at 4958.251 kb on + strand at 4958.251 kb on + strand at 4958.252 kb on - strand at 4958.653 kb on + strand at 4958.654 kb on - strand, within Pf1N1B4_4496 at 4958.685 kb on + strand at 4958.685 kb on + strand, within Pf1N1B4_4496 at 4958.686 kb on - strand at 4958.742 kb on + strand at 4958.743 kb on - strand at 4958.848 kb on - strand at 4958.889 kb on + strand at 4958.889 kb on + strand, within Pf1N1B4_4497
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t1 remove 4,955,873 - Pf1N1B4_4493 0.14 +0.0 4,955,982 + Pf1N1B4_4493 0.25 +0.0 4,956,128 + Pf1N1B4_4493 0.41 -1.1 4,956,321 - Pf1N1B4_4493 0.61 +0.0 4,956,378 + Pf1N1B4_4493 0.67 -1.8 4,956,378 + +0.0 4,956,378 + Pf1N1B4_4493 0.67 -3.2 4,956,379 - -2.6 4,956,379 - Pf1N1B4_4493 0.67 -1.0 4,956,379 - Pf1N1B4_4493 0.67 -1.4 4,956,379 - Pf1N1B4_4493 0.67 -1.8 4,956,379 - +0.0 4,956,504 + Pf1N1B4_4493 0.80 +0.0 4,956,505 - Pf1N1B4_4493 0.80 +0.0 4,956,505 - Pf1N1B4_4493 0.80 +0.0 4,956,947 - Pf1N1B4_4494 0.18 -3.2 4,957,176 + Pf1N1B4_4494 0.37 +0.0 4,957,674 + +0.0 4,957,887 + -2.1 4,957,900 - +0.0 4,957,949 + -2.5 4,957,974 + +0.0 4,957,986 - -2.6 4,958,007 + +0.0 4,958,008 - -2.2 4,958,010 + -4.7 4,958,045 + +0.0 4,958,045 + -2.1 4,958,045 + -3.3 4,958,190 + Pf1N1B4_4495 0.87 +0.0 4,958,193 + Pf1N1B4_4495 0.89 +0.0 4,958,194 - -3.2 4,958,194 - -1.8 4,958,251 + +0.0 4,958,251 + -2.4 4,958,252 - +0.0 4,958,653 + +0.0 4,958,654 - Pf1N1B4_4496 0.74 +0.0 4,958,685 + -1.9 4,958,685 + Pf1N1B4_4496 0.81 +0.0 4,958,686 - +0.0 4,958,742 + +0.0 4,958,743 - +0.0 4,958,848 - +0.0 4,958,889 + -3.7 4,958,889 + Pf1N1B4_4497 0.13 -3.7
Or see this region's nucleotide sequence