Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4094

Experiment: Nitrate reduction, replicate C t1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4093 and Pf1N1B4_4094 are separated by 225 nucleotidesPf1N1B4_4094 and Pf1N1B4_4095 are separated by 10 nucleotides Pf1N1B4_4093 - Alkyl hydroperoxide reductase subunit C-like protein, at 4,542,334 to 4,542,987 _4093 Pf1N1B4_4094 - Uncharacterized conserved protein, at 4,543,213 to 4,543,710 _4094 Pf1N1B4_4095 - Transcriptional regulator, LacI family, at 4,543,721 to 4,544,722 _4095 Position (kb) 4543 4544Strain fitness (log2 ratio) -3 -2 -1 0 1at 4542.240 kb on + strandat 4542.240 kb on + strandat 4542.243 kb on - strandat 4542.307 kb on + strandat 4542.320 kb on + strandat 4542.320 kb on + strandat 4542.484 kb on + strand, within Pf1N1B4_4093at 4542.656 kb on - strand, within Pf1N1B4_4093at 4542.712 kb on - strand, within Pf1N1B4_4093at 4542.770 kb on - strand, within Pf1N1B4_4093at 4542.770 kb on - strand, within Pf1N1B4_4093at 4542.929 kb on - strandat 4542.958 kb on + strandat 4542.959 kb on - strandat 4543.021 kb on + strandat 4543.167 kb on + strandat 4543.167 kb on + strandat 4543.176 kb on - strandat 4543.176 kb on - strandat 4543.380 kb on - strand, within Pf1N1B4_4094at 4543.445 kb on - strand, within Pf1N1B4_4094at 4543.536 kb on - strand, within Pf1N1B4_4094at 4543.928 kb on - strand, within Pf1N1B4_4095at 4544.388 kb on + strand, within Pf1N1B4_4095at 4544.639 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t1
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4,542,240 + +0.0
4,542,240 + -1.4
4,542,243 - +0.0
4,542,307 + -1.3
4,542,320 + +0.0
4,542,320 + -3.6
4,542,484 + Pf1N1B4_4093 0.23 +0.0
4,542,656 - Pf1N1B4_4093 0.49 -2.5
4,542,712 - Pf1N1B4_4093 0.58 +0.0
4,542,770 - Pf1N1B4_4093 0.67 +0.0
4,542,770 - Pf1N1B4_4093 0.67 +0.0
4,542,929 - +0.0
4,542,958 + +0.0
4,542,959 - +0.0
4,543,021 + +0.0
4,543,167 + +0.0
4,543,167 + +0.0
4,543,176 - -2.1
4,543,176 - -2.2
4,543,380 - Pf1N1B4_4094 0.34 -2.2
4,543,445 - Pf1N1B4_4094 0.47 +0.0
4,543,536 - Pf1N1B4_4094 0.65 -3.1
4,543,928 - Pf1N1B4_4095 0.21 +0.0
4,544,388 + Pf1N1B4_4095 0.67 -2.2
4,544,639 + +0.0

Or see this region's nucleotide sequence