Experiment: Nitrate reduction, replicate C t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3975 and Pf1N1B4_3976 are separated by 38 nucleotides Pf1N1B4_3976 and Pf1N1B4_3977 overlap by 4 nucleotides Pf1N1B4_3977 and Pf1N1B4_3978 are separated by 155 nucleotides
Pf1N1B4_3975 - Transcriptional regulator, IclR family, at 4,416,299 to 4,417,135
_3975
Pf1N1B4_3976 - Transmembrane regulator protein PrtR, at 4,417,174 to 4,417,926
_3976
Pf1N1B4_3977 - RNA polymerase sigma-54 factor RpoN, at 4,417,923 to 4,418,483
_3977
Pf1N1B4_3978 - Catalase (EC 1.11.1.6), at 4,418,639 to 4,419,688
_3978
Position (kb) 4417
4418 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 4416.214 kb on + strand at 4416.233 kb on + strand at 4416.254 kb on - strand at 4416.812 kb on + strand at 4416.812 kb on + strand, within Pf1N1B4_3975 at 4416.845 kb on + strand, within Pf1N1B4_3975 at 4416.918 kb on + strand, within Pf1N1B4_3975 at 4416.919 kb on - strand, within Pf1N1B4_3975 at 4416.919 kb on - strand, within Pf1N1B4_3975 at 4417.238 kb on + strand at 4417.238 kb on + strand at 4417.238 kb on + strand at 4417.239 kb on - strand at 4417.239 kb on - strand at 4417.262 kb on + strand, within Pf1N1B4_3976 at 4417.318 kb on - strand, within Pf1N1B4_3976 at 4417.337 kb on + strand, within Pf1N1B4_3976 at 4417.337 kb on + strand, within Pf1N1B4_3976 at 4417.337 kb on + strand, within Pf1N1B4_3976 at 4417.337 kb on + strand, within Pf1N1B4_3976 at 4417.337 kb on + strand at 4417.338 kb on - strand at 4417.370 kb on + strand, within Pf1N1B4_3976 at 4417.370 kb on + strand, within Pf1N1B4_3976 at 4417.370 kb on + strand at 4417.370 kb on + strand, within Pf1N1B4_3976 at 4417.371 kb on - strand, within Pf1N1B4_3976 at 4417.371 kb on - strand at 4417.371 kb on - strand, within Pf1N1B4_3976 at 4417.371 kb on - strand, within Pf1N1B4_3976 at 4417.371 kb on - strand, within Pf1N1B4_3976 at 4417.371 kb on - strand, within Pf1N1B4_3976 at 4417.371 kb on - strand, within Pf1N1B4_3976 at 4417.371 kb on - strand, within Pf1N1B4_3976 at 4417.371 kb on - strand, within Pf1N1B4_3976 at 4417.409 kb on - strand, within Pf1N1B4_3976 at 4417.498 kb on - strand, within Pf1N1B4_3976 at 4417.521 kb on - strand, within Pf1N1B4_3976 at 4417.521 kb on - strand, within Pf1N1B4_3976 at 4417.521 kb on - strand, within Pf1N1B4_3976 at 4417.838 kb on + strand, within Pf1N1B4_3976 at 4417.838 kb on + strand, within Pf1N1B4_3976 at 4417.839 kb on - strand, within Pf1N1B4_3976 at 4417.839 kb on - strand, within Pf1N1B4_3976 at 4417.839 kb on - strand, within Pf1N1B4_3976 at 4417.839 kb on - strand, within Pf1N1B4_3976 at 4417.839 kb on - strand, within Pf1N1B4_3976 at 4417.887 kb on - strand at 4418.051 kb on + strand, within Pf1N1B4_3977 at 4418.051 kb on + strand, within Pf1N1B4_3977 at 4418.051 kb on + strand, within Pf1N1B4_3977 at 4418.051 kb on + strand, within Pf1N1B4_3977 at 4418.051 kb on + strand, within Pf1N1B4_3977 at 4418.052 kb on - strand, within Pf1N1B4_3977 at 4418.053 kb on + strand, within Pf1N1B4_3977 at 4418.053 kb on + strand at 4418.053 kb on + strand, within Pf1N1B4_3977 at 4418.053 kb on + strand, within Pf1N1B4_3977 at 4418.054 kb on - strand, within Pf1N1B4_3977 at 4418.441 kb on + strand at 4418.449 kb on - strand at 4418.529 kb on + strand at 4418.598 kb on + strand at 4418.598 kb on + strand at 4418.598 kb on + strand at 4418.598 kb on + strand at 4418.599 kb on - strand at 4418.714 kb on + strand at 4418.791 kb on - strand, within Pf1N1B4_3978 at 4418.809 kb on + strand, within Pf1N1B4_3978
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t1 remove 4,416,214 + -2.6 4,416,233 + +0.0 4,416,254 - +0.0 4,416,812 + +0.0 4,416,812 + Pf1N1B4_3975 0.61 -2.9 4,416,845 + Pf1N1B4_3975 0.65 +0.0 4,416,918 + Pf1N1B4_3975 0.74 +0.0 4,416,919 - Pf1N1B4_3975 0.74 +0.0 4,416,919 - Pf1N1B4_3975 0.74 -3.0 4,417,238 + -3.1 4,417,238 + -2.8 4,417,238 + +0.0 4,417,239 - -1.8 4,417,239 - +0.0 4,417,262 + Pf1N1B4_3976 0.12 +0.0 4,417,318 - Pf1N1B4_3976 0.19 +0.0 4,417,337 + Pf1N1B4_3976 0.22 +0.0 4,417,337 + Pf1N1B4_3976 0.22 -4.7 4,417,337 + Pf1N1B4_3976 0.22 +0.0 4,417,337 + Pf1N1B4_3976 0.22 +0.0 4,417,337 + -2.7 4,417,338 - +0.0 4,417,370 + Pf1N1B4_3976 0.26 +0.0 4,417,370 + Pf1N1B4_3976 0.26 +0.0 4,417,370 + +0.0 4,417,370 + Pf1N1B4_3976 0.26 -3.8 4,417,371 - Pf1N1B4_3976 0.26 +0.0 4,417,371 - +0.0 4,417,371 - Pf1N1B4_3976 0.26 -4.6 4,417,371 - Pf1N1B4_3976 0.26 -4.6 4,417,371 - Pf1N1B4_3976 0.26 -4.6 4,417,371 - Pf1N1B4_3976 0.26 +0.0 4,417,371 - Pf1N1B4_3976 0.26 -4.2 4,417,371 - Pf1N1B4_3976 0.26 -3.3 4,417,371 - Pf1N1B4_3976 0.26 -5.3 4,417,409 - Pf1N1B4_3976 0.31 -2.2 4,417,498 - Pf1N1B4_3976 0.43 +0.0 4,417,521 - Pf1N1B4_3976 0.46 -3.1 4,417,521 - Pf1N1B4_3976 0.46 +0.0 4,417,521 - Pf1N1B4_3976 0.46 +0.0 4,417,838 + Pf1N1B4_3976 0.88 -3.3 4,417,838 + Pf1N1B4_3976 0.88 -1.0 4,417,839 - Pf1N1B4_3976 0.88 -3.9 4,417,839 - Pf1N1B4_3976 0.88 -3.2 4,417,839 - Pf1N1B4_3976 0.88 -3.9 4,417,839 - Pf1N1B4_3976 0.88 +0.0 4,417,839 - Pf1N1B4_3976 0.88 -1.2 4,417,887 - +0.0 4,418,051 + Pf1N1B4_3977 0.23 +0.0 4,418,051 + Pf1N1B4_3977 0.23 -2.0 4,418,051 + Pf1N1B4_3977 0.23 +0.0 4,418,051 + Pf1N1B4_3977 0.23 +0.0 4,418,051 + Pf1N1B4_3977 0.23 +0.0 4,418,052 - Pf1N1B4_3977 0.23 +0.0 4,418,053 + Pf1N1B4_3977 0.23 +0.0 4,418,053 + +0.0 4,418,053 + Pf1N1B4_3977 0.23 -3.0 4,418,053 + Pf1N1B4_3977 0.23 +0.0 4,418,054 - Pf1N1B4_3977 0.23 -1.3 4,418,441 + +0.0 4,418,449 - +0.0 4,418,529 + +0.0 4,418,598 + +0.0 4,418,598 + +0.0 4,418,598 + -1.7 4,418,598 + +0.0 4,418,599 - +0.0 4,418,714 + +0.0 4,418,791 - Pf1N1B4_3978 0.14 -2.3 4,418,809 + Pf1N1B4_3978 0.16 +0.0
Or see this region's nucleotide sequence