Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3583

Experiment: Nitrate reduction, replicate C t1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3582 and Pf1N1B4_3583 are separated by 16 nucleotidesPf1N1B4_3583 and Pf1N1B4_3584 are separated by 51 nucleotides Pf1N1B4_3582 - C-methyltransferase, at 3,992,603 to 3,993,673 _3582 Pf1N1B4_3583 - Cephalosporin hydroxylase, at 3,993,690 to 3,994,427 _3583 Pf1N1B4_3584 - O-antigen biosynthesis protein, at 3,994,479 to 3,997,415 _3584 Position (kb) 3993 3994 3995Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3992.768 kb on + strand, within Pf1N1B4_3582at 3992.769 kb on - strand, within Pf1N1B4_3582at 3992.780 kb on + strand, within Pf1N1B4_3582at 3993.039 kb on + strand, within Pf1N1B4_3582at 3993.263 kb on + strand, within Pf1N1B4_3582at 3993.599 kb on + strandat 3993.599 kb on + strandat 3993.600 kb on - strandat 3993.635 kb on + strandat 3993.636 kb on - strandat 3993.687 kb on - strandat 3993.817 kb on - strandat 3993.817 kb on - strand, within Pf1N1B4_3583at 3993.817 kb on - strand, within Pf1N1B4_3583at 3993.823 kb on - strandat 3993.957 kb on + strand, within Pf1N1B4_3583at 3994.064 kb on + strand, within Pf1N1B4_3583at 3994.065 kb on - strand, within Pf1N1B4_3583at 3994.065 kb on - strand, within Pf1N1B4_3583at 3994.200 kb on + strand, within Pf1N1B4_3583at 3994.228 kb on - strand, within Pf1N1B4_3583at 3994.228 kb on - strand, within Pf1N1B4_3583at 3994.329 kb on + strand, within Pf1N1B4_3583at 3994.330 kb on - strand, within Pf1N1B4_3583at 3994.588 kb on - strandat 3994.590 kb on + strandat 3994.590 kb on + strandat 3994.591 kb on - strandat 3994.591 kb on - strandat 3994.591 kb on - strandat 3994.599 kb on + strandat 3994.600 kb on - strandat 3994.701 kb on + strandat 3994.702 kb on - strandat 3994.702 kb on - strandat 3994.755 kb on + strandat 3994.755 kb on + strandat 3994.755 kb on + strandat 3994.755 kb on + strandat 3994.839 kb on + strand, within Pf1N1B4_3584at 3994.844 kb on + strand, within Pf1N1B4_3584at 3994.844 kb on + strand, within Pf1N1B4_3584at 3994.844 kb on + strand, within Pf1N1B4_3584at 3994.844 kb on + strand, within Pf1N1B4_3584at 3994.845 kb on - strand, within Pf1N1B4_3584at 3994.845 kb on - strand, within Pf1N1B4_3584at 3994.869 kb on + strand, within Pf1N1B4_3584at 3994.869 kb on + strand, within Pf1N1B4_3584at 3994.870 kb on - strand, within Pf1N1B4_3584at 3994.870 kb on - strand, within Pf1N1B4_3584at 3994.887 kb on + strand, within Pf1N1B4_3584at 3994.887 kb on + strand, within Pf1N1B4_3584at 3994.888 kb on - strand, within Pf1N1B4_3584at 3994.888 kb on - strand, within Pf1N1B4_3584at 3994.888 kb on - strand, within Pf1N1B4_3584at 3994.888 kb on - strand, within Pf1N1B4_3584at 3994.888 kb on - strand, within Pf1N1B4_3584at 3994.896 kb on + strand, within Pf1N1B4_3584at 3994.897 kb on - strandat 3994.899 kb on + strand, within Pf1N1B4_3584at 3994.918 kb on + strand, within Pf1N1B4_3584at 3994.965 kb on + strandat 3994.966 kb on - strand, within Pf1N1B4_3584at 3994.977 kb on + strand, within Pf1N1B4_3584at 3994.977 kb on + strand, within Pf1N1B4_3584at 3994.978 kb on - strand, within Pf1N1B4_3584at 3994.978 kb on - strand, within Pf1N1B4_3584at 3994.978 kb on - strandat 3994.978 kb on - strand, within Pf1N1B4_3584at 3994.992 kb on + strand, within Pf1N1B4_3584at 3994.993 kb on - strand, within Pf1N1B4_3584at 3995.028 kb on + strand, within Pf1N1B4_3584at 3995.082 kb on + strand, within Pf1N1B4_3584at 3995.083 kb on - strand, within Pf1N1B4_3584at 3995.083 kb on - strand, within Pf1N1B4_3584at 3995.083 kb on - strand, within Pf1N1B4_3584at 3995.085 kb on + strand, within Pf1N1B4_3584at 3995.085 kb on + strand, within Pf1N1B4_3584at 3995.085 kb on + strand, within Pf1N1B4_3584at 3995.086 kb on - strand, within Pf1N1B4_3584at 3995.086 kb on - strand, within Pf1N1B4_3584at 3995.086 kb on - strand, within Pf1N1B4_3584at 3995.127 kb on + strand, within Pf1N1B4_3584at 3995.127 kb on + strand, within Pf1N1B4_3584at 3995.127 kb on + strand, within Pf1N1B4_3584at 3995.127 kb on + strand, within Pf1N1B4_3584at 3995.128 kb on - strand, within Pf1N1B4_3584at 3995.427 kb on + strand, within Pf1N1B4_3584at 3995.427 kb on + strand, within Pf1N1B4_3584at 3995.427 kb on + strand, within Pf1N1B4_3584at 3995.427 kb on + strand, within Pf1N1B4_3584at 3995.427 kb on + strand, within Pf1N1B4_3584at 3995.427 kb on + strand, within Pf1N1B4_3584

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t1
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3,992,768 + Pf1N1B4_3582 0.15 -2.2
3,992,769 - Pf1N1B4_3582 0.15 +0.0
3,992,780 + Pf1N1B4_3582 0.17 +0.0
3,993,039 + Pf1N1B4_3582 0.41 -3.5
3,993,263 + Pf1N1B4_3582 0.62 +0.0
3,993,599 + -3.2
3,993,599 + -2.0
3,993,600 - +0.0
3,993,635 + +0.0
3,993,636 - -4.4
3,993,687 - +0.0
3,993,817 - -2.4
3,993,817 - Pf1N1B4_3583 0.17 -3.9
3,993,817 - Pf1N1B4_3583 0.17 +0.0
3,993,823 - +0.0
3,993,957 + Pf1N1B4_3583 0.36 +0.0
3,994,064 + Pf1N1B4_3583 0.51 -3.7
3,994,065 - Pf1N1B4_3583 0.51 -3.7
3,994,065 - Pf1N1B4_3583 0.51 +0.0
3,994,200 + Pf1N1B4_3583 0.69 +0.0
3,994,228 - Pf1N1B4_3583 0.73 +0.0
3,994,228 - Pf1N1B4_3583 0.73 +0.0
3,994,329 + Pf1N1B4_3583 0.87 -3.5
3,994,330 - Pf1N1B4_3583 0.87 +0.0
3,994,588 - -2.6
3,994,590 + +0.0
3,994,590 + -2.5
3,994,591 - -1.9
3,994,591 - -3.2
3,994,591 - -3.4
3,994,599 + +0.0
3,994,600 - -2.9
3,994,701 + +0.0
3,994,702 - +0.0
3,994,702 - +0.0
3,994,755 + +0.0
3,994,755 + +0.0
3,994,755 + +0.0
3,994,755 + -2.7
3,994,839 + Pf1N1B4_3584 0.12 +0.0
3,994,844 + Pf1N1B4_3584 0.12 +0.0
3,994,844 + Pf1N1B4_3584 0.12 -2.4
3,994,844 + Pf1N1B4_3584 0.12 +0.0
3,994,844 + Pf1N1B4_3584 0.12 +0.0
3,994,845 - Pf1N1B4_3584 0.12 +0.0
3,994,845 - Pf1N1B4_3584 0.12 -2.1
3,994,869 + Pf1N1B4_3584 0.13 +0.0
3,994,869 + Pf1N1B4_3584 0.13 +0.0
3,994,870 - Pf1N1B4_3584 0.13 -3.3
3,994,870 - Pf1N1B4_3584 0.13 +0.0
3,994,887 + Pf1N1B4_3584 0.14 -1.6
3,994,887 + Pf1N1B4_3584 0.14 -2.2
3,994,888 - Pf1N1B4_3584 0.14 -1.2
3,994,888 - Pf1N1B4_3584 0.14 +0.0
3,994,888 - Pf1N1B4_3584 0.14 +0.0
3,994,888 - Pf1N1B4_3584 0.14 -1.8
3,994,888 - Pf1N1B4_3584 0.14 -1.9
3,994,896 + Pf1N1B4_3584 0.14 -3.6
3,994,897 - +0.0
3,994,899 + Pf1N1B4_3584 0.14 +0.0
3,994,918 + Pf1N1B4_3584 0.15 +0.0
3,994,965 + +0.0
3,994,966 - Pf1N1B4_3584 0.17 +0.0
3,994,977 + Pf1N1B4_3584 0.17 -1.0
3,994,977 + Pf1N1B4_3584 0.17 +0.0
3,994,978 - Pf1N1B4_3584 0.17 -1.2
3,994,978 - Pf1N1B4_3584 0.17 -1.2
3,994,978 - +0.0
3,994,978 - Pf1N1B4_3584 0.17 +0.0
3,994,992 + Pf1N1B4_3584 0.17 +0.0
3,994,993 - Pf1N1B4_3584 0.18 -2.2
3,995,028 + Pf1N1B4_3584 0.19 -1.6
3,995,082 + Pf1N1B4_3584 0.21 +0.0
3,995,083 - Pf1N1B4_3584 0.21 +0.0
3,995,083 - Pf1N1B4_3584 0.21 +0.0
3,995,083 - Pf1N1B4_3584 0.21 -2.5
3,995,085 + Pf1N1B4_3584 0.21 +0.0
3,995,085 + Pf1N1B4_3584 0.21 -2.8
3,995,085 + Pf1N1B4_3584 0.21 +0.0
3,995,086 - Pf1N1B4_3584 0.21 +0.0
3,995,086 - Pf1N1B4_3584 0.21 +0.0
3,995,086 - Pf1N1B4_3584 0.21 +0.0
3,995,127 + Pf1N1B4_3584 0.22 -1.9
3,995,127 + Pf1N1B4_3584 0.22 -2.1
3,995,127 + Pf1N1B4_3584 0.22 +0.0
3,995,127 + Pf1N1B4_3584 0.22 +0.0
3,995,128 - Pf1N1B4_3584 0.22 +0.0
3,995,427 + Pf1N1B4_3584 0.32 +0.0
3,995,427 + Pf1N1B4_3584 0.32 +0.0
3,995,427 + Pf1N1B4_3584 0.32 +0.0
3,995,427 + Pf1N1B4_3584 0.32 -3.5
3,995,427 + Pf1N1B4_3584 0.32 -2.3
3,995,427 + Pf1N1B4_3584 0.32 -2.3

Or see this region's nucleotide sequence